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Inhibition of Dendrobium bicaudatum Reinw. ex Lindl growth using Paclobutrazol for in vitro conservation Ida Ayu Putri Darmawati Ida Ayu; Yuyun Fitriani; Gede Wijana
Jurnal Hortikultura Indonesia (JHI) Vol 13 No 1 (2022): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.13.1.29-34

Abstract

D. bicaudatum orchid has become threatened with extinction. Environmental damage due to human activities and overcollection of orchids has triggered the extinction of orchids in their natural habitat. In situ and ex situ conservation measures need to be taken to reduce the threat of extinction for orchids. This research aims to investigate the effect of paclobutrazol (PBZ) on culture mediums as a plant growth inhibitor, one of which is in vitro conservation measures, and was conducted experimentally using a completely randomized design. The independent variables were the concentrations of PBZ of 0, 1, 3, and 5 mg L-1, respectively, which were added to the growing medium in the form of solid MS medium. The medium-term conservation of D.bicaudatum orchids with the addition of paclobutrazol exhibited a very significant result. Inhibition of vegetative growth such as the number of leaves and root length was exhibited by 1-5 mg L-1 PBZ, while the stem diameter, as one of the dependent variables, increased more in all treatment groups than in the control group. Reduced number of roots occurred in the groups of treatments of 1 mg L-1 PBZ and 3 mg L-1 PBZ. Keywords: extinction, in Vitro culture, reduction
Growth Analysis of Differences in Shallot (Allium ascalonicum L.) Seedling Size I Gusti Ngurah Wahyu Arsadiarta; Gede Wijana; I Gusti Alit Gunadi
Agro Bali : Agricultural Journal Vol 7, No 3 (2024)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v7i3.1863

Abstract

Choosing the right seed size is essential in shallot cultivation. Using good-quality seeds will increase the growth of shallots. Through growth analysis of various sizes of shallot seedlings, information will be obtained about the growth potential of shallots. This research aims to compare the growth patterns of shallot seedlings of varying sizes. The research was carried out from August to September 2023 at Br. Aseman, Sedang Village, Abiansemal District, Badung Regency, Bali and at the Plant Breeding and Seed Technology Laboratory, Faculty of Agriculture, Udayana University, Denpasar. This research used a one-factor randomized block design (RBD) with different treatment sizes of shallot seedlings, namely 3 g, 5 g, 7 g, 9 g, and 11 g. Each treatment was repeated 5 times, yielding 25 experimental units. The results showed that differences in the size of shallot seedlings very significantly influenced the initial growth of the seedlings (3-15 DAP). The heavier the weight of the shallot seeds, the higher the growth of the shallots. The plant growth analysis metrics, relative growth rate and net assimilation rate, reveal that the results do not differ significantly across observation time intervals. The treatment using seed size 11 g showed the highest increase in each parameter compared to other treatments at each observation time interval.
Indigenous arbuscular mycorrhizal fungi at tangerine cv. Tejakula (Citrus reticulata cv. Tejakula) plantations, their colonization of the roots, and their effect on soil fertility Wijana, Gede; Rai, I Nyoman; Mayadewi, Ni Nyoman Ari; Astiari, Ni Komang Alit
Journal of Degraded and Mining Lands Management Vol. 12 No. 2 (2025)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2025.122.7173

Abstract

Tangerines (Citrus reticulata var. Tejakula) were destroyed in 1984 by CVPD, allegedly because the use of chemicals was too intensive, resulting in land degradation. Since ten years ago, these oranges have been successfully cultivated again using healthy organic cultivation, but farmers have difficulty getting organic fertilizer. This research aimed to identify and isolate the Arbuscular Mycorrhizal Fungi (AMF) on tangerines cv. Tejakula, their colonization of the roots and effect on soil fertility. Exploration was conducted by collecting root and soil samples in tangerine cv. Tejakula production centers are in the Tejakula District, Buleleng Regency. Spore isolation was conducted using wet filtration and centrifugation techniques, while root colonization by AMF was carried out using the staining method with trypan blue. The isolated spores are composited into AMF biofertilizer using volcanic sand as a carrier medium. The biofertilizer was then tested for its effect on soil fertility with three treatment levels of AMF spore doses (0, 20, and 40 spores/50 g of carrier media per plant). The results of exploration, isolation, and identification showed that three genera and six species of AMF were found. The spore density ranged from 17 to 32 per 100 g of soil (mean 24.17 ± 2.02 spores). The average root colonization by AMF was very high, ranging from 90 to 100%, and the application of AMF biofertilizer at a dose of 20 and 40 spores per plant provided soil fertility was much better than the control (0 AMF spores per plant).
Effect of Fertilization Package on Vegetative Growth of Tejakula Tangerine (Citrus reticulata cv. Tejakula) After Transplanting to the Field I Nyoman Rai; Gede Wijana; Jaya Duarsa Mintarajasa
Agro Bali : Agricultural Journal Vol 8, No 1 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i1.1827

Abstract

Farmers have applied fertilization but have not achieved optimal vegetative growth of Tejakula tangerine plants. This study aims to find a combination of fertilization packages that can provide the best response to vegetative growth and improve the soil quality of Tejakula tangerine plants after moving to the field. The research was conducted in Tembok Village, Tejakula District, Buleleng Regency, Bali from  July to December 2023. The experiment used a one-factor Randomized Block Design (RBD) with fertilization package treatment consisting of 3 levels, namely: 5 kg cow manure per tree, 5 kg cow manure + mycorrhizal biofertilizer 20 spores/50 g volcanic sand carrier + probiotic liquid organic fertilizer 20% concentration per tree and 5 kg cow manure + urea 50 g + SP-36 25 g + KCl 20 g per tree. Each treatment was repeated 8 times, resulting in 24 experimental units. Observational data were analyzed using the Analysis of Variance (ANOVA) test, and further tests were carried out using the Least Significant Difference (LSD) test. The results showed that the fertilizer package treatment had a very significant effect on the variable number of primary branches and leaf P nutrient content, significantly affected the variable number of plant stem diameter and leaf chlorophyll content, but had no significant effect on the variable number of plant height, relative water content (RWC) of leaves and leaf color. The package of cow manure + inorganic fertilizer influenced the vegetative growth of Tejakula tangerine after transplanting to the field very significantly on the increase in primary branches, significantly influenced the increase in plant stem diameter and leaf chlorophyll content. The organic fertilization package + mycorrhizal biofertilizer + probiotic liquid organic fertilizer can improve the soil quality of Tejakula tangerine plants.
Effectiveness of Various Scarification Methods on Breaking Seed Dormancy in Some Types of Palm (Arecaceae) Ketut Weda Setora; Gede Wijana; Ida Ayu Putri Darmawati
Agro Bali : Agricultural Journal Vol 8, No 1 (2025)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37637/ab.v8i1.1995

Abstract

The palm breeding and cultivation challenges are mainly related to the seed germination process. Various factors, including seed dormancy, often influence this process. Various scarification methods and several types of palms that can play a role in breaking dormancy need to be tried. This research aims to determine the best dormancy-breaking scarification method for various palm seeds. This research was carried out in Bakung Village, Sukasada District, Buleleng Regency, from January to March 2024, using a Nested Design comprising two factors. The first factor is that the type of palm seed consists of four levels: Putri palm, Pinang palm, Squirrel Tail palm, and Green palm. The second factor consists of six levels, namely control method (without peeling), peel scarification method, peel scarification method+warm water, peel scarification method+H2SO4, peel scarification method+KNO3, peel scarification method+GA3. The results of the research showed that the scarification method had a significant effect (p<0,05) on the percentage of sprouts and shoot length. Very significant effect (p<0,01) on the variables of germination, maximum growth potential, seed growth speed, and root length, had an not significant effect on the synchronization of seed growth. This research concludes that the scarification method affects breaking seed dormancy in several types of palm, except for the areca palm.
Respons tanaman kelapa sawit (Elaeis guineensis Jacq) terhadap cekaman kekeringan Respons of oil palm (Elaeis guineensis Jacq) to water stress Nurita TORUAN-MATHIUS; Gede WIJANA; Edi GUHARJA; Hajrial ASWIDINNOOR; Sudirman YAHYA; . SUBRONTO
Menara Perkebunan Vol. 69 No. 2: 69 (2), 2001
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v69i2.166

Abstract

SummaryWater stress affect many physiological andbiochemical processes of oil palm. A series ofexperiments were conducted to characterize thewater stress-induced changes in physiologicalrespons of oil palm to water stress, in glass housecondition. The experiment consisted of (1)permanent leaf wilting point measured based onsoil water content, leaf water content, specificleaf area and leaf water potential . Plants wereconducted by termination of watering to theplants, and control plants were maintained wellwatered during 0,3,6,9,12,15,18 and 21 days ofMK356 and MK365 clones. Experiment (2)effect of water stress on changes of leaf waterpotential, protein bands pattern, proline,glycine-betaine, osmotical sugar, and abcisicacid (ABA) of MK356 and MK365 clones.Water stress was induced by termination ofwatering to the plants and maintained wellwatered during 0, 7,14, and 18 days.Experiment (3) changes of protein bands patternby total protein and electrophoresis SDS-PAGEand SDS-PAGE 2D protein. of H2(D10DxD8D)x(L9TxL2T); H12 (D8D Self) x(L9T x L2T). H3 and H9 (BJ028D x BJ2117P)hybrids. H2 and H12, H3 and H9 potentiallytolerant and untolerant to water stress,respectively. The results showed that permanentwilting point reached in 18 days of water stress.Water stress caused the decreased soil watercontent, leaf water potential, leaf water content,relative leaf water content , and relative leafarea of two clones. Water potential, leaf watecontent dan relative leaf water content ofMK365 decrease faster compare with MK356.Soil water content sharply decrease after 6 hoursand in 18 days of water stress leaf waterpotential value < - 2.55 Mpa. Proline, glycine-betaine and glucose content were affect by waterstress. Interaction among water stress and cloneswere significantly appear in stachiose content.Leaf water potential values decrease, whereasproline, ABA and glycine-betaine contentsincrease during water stress especially inMK356. Generally showed that ABA content inMK356 higher than MK 365. The differencesresponses of MK356 with MK 365 obtained fromprolin,xylose and ABA content. Induction of newprotein pI 4.7-36 kDa, pI5.3-34 kDa, pI 4.6-32kDa and pI 5.3-36 kDa obtained from hybridspotentially tolerant to water strees, none inuntolerant hybrids.RingkasanCekaman kekeringan mempengaruhiproses fisiologis dan biokimia tanaman kelapasawit. Serangkaian percobaan bertujuan untukmengkarakterisasi perubahan fisiologis tanamankelapa sawit terhadap cekaman kekeringan,dalam kondisi rumah kaca telah dilakukan.Percobaan terdiri atas (1) penetapan titik layupermanen, berdasarkan perubahan potensial airdaun, kadar air daun, kadar air daun relatif, danluas daun relatif dengan perlakuan tanpa dandengan penyiraman selama 0, 3, 6, 9, 12, 15, 18dan 21 hari. Percobaan (2) penetapan perubahankadar prolin, glisin-betain, gula-gula osmotikaldan asam absisik (ABA), terhadap cekamankekeringan. Perlakuan adalah tanpa dan denganpenyiraman selama 0, 7, 14, dan 18 hari.Percobaan (3) analisis perubahan pola pita proteindaun hibrida H2 (D10DxD8D)x(L9TxL2T); H12(D8D Self) x (L9T x L2T). H3 dan H9 (BJ028Dx BJ2117P) terhadap cekaman kekeringan dengantotal protein, dan pola pita protein dengan SDSPAGE dan SDS-PAGE 2D. H2 dan H12 serta H3dan H9 masing-masing berpotensi toleran danpeka terhadap cekaman kekeringan. Hasil yangdiperoleh menunjukkan bahwa titik layupermanen dicapai pada hari ke 18 setelah dibericekaman kekeringan. Cekaman kekeringanmenurunkan kadar air tanah media tumbuh,potensial air daun, kadar air daun, kadar air daunrelatif, dan luas daun relatif untuk kedua klon.Potensial air daun, kadar air daun dan kadar airdaun relatif klon MK365 menurun lebih cepatdibandingkan dengan klon MK356. Kadar airtanah menurun tajam setelah 6 hari dibericekaman air dan potensial air daun mencapai<-2.55 MPa pada 18 hari setelah diberi cekaman.Cekaman kekeringan nyata berpengaruh terhadapkadar prolin, glisin betain dan glukosa. Interaksiantar lama cekaman kekeringan dan perbedaanklon diperoleh pada perubahan gula stahiosa.Tampak bahwa semakin menurun nilai potensialair daun menyebabkan kadar prolin semakinmeningkat. Hal yang sebaliknya terjadi terhadapkadar glisin-betain yang mengalami penurunanterutama untuk klon MK356. Kadar ABAMK356 dan MK365 meningkat sejalan dengansemakin lama diberi cekaman. Secara umumtampak bahwa kadar ABA pada MK356 lebihtinggi dibandingkan dengan MK 365. Perbedaanrespons klon MK356 dengan MK 365 terjadipada kadar prolin, gula silosa dan ABA.Hibridaberpotensi toleran memberikan respon terhadapcekaman kekeringan dengan menginduksi proteinbaru pI 4,7-36 kDa, pI5,3-34 kDa, pI 4,6-32 kDadan pI 5,3- 36 kDa, sedangkan pada hibridayang berpotensi peka protein tersebut tidakditemukan
Co-Authors . SUBRONTO Abd. Rasyid Syamsuri ADINDA RIZKI NURANA ANAK AGUNG MADE ASTININGSIH Anandamurti, I Wayan Krisnayogi ANTONIUS ALI Butar, Adril Persada Butar Cokorda Gede Alit Semarajaya DARJO DARJO DAVID HARYSUSANTO DECIO ARISTA ESTANISLAU DA COSTA RIBEIRO Dewi, Ni Putu Elvina Kusuma Edi GUHARJA EUSEBIO GOMES Frangki Rionardo Lay GEDE MENAKA ADNYANA Gusti Ngurah Alit Susanta Wirya HAJRIAL ASWIDINNOOR Henrietto Innosensius Prasetyo HENRIETTO INNOSENSIUS PRASETYO I GEDE KRISNA PRATAMA PUTRA I GUSTI AGUNG BAGUS SURADARMA I Gusti Alit Gunadi I Gusti Ayu Mas Sri Agung I Gusti Ngurah Bagus I GUSTI NGURAH RAKA I GUSTI NGURAH SANTOSA I Gusti Ngurah Wahyu Arsadiarta I GUSTI RAKAWISNU I KADEK EKADANA I KADEK WAHYU WIDIATMIKA I Ketut Suada I KOMANG AGUS SUPRIANA I MADE AGUS KRISNA I MADE SUDANA I MADE SUDARMA I MADE SUDARMA I Made Sukewijaya I Made Surya Adi Putra Putra I MADE WIDIADA I N. RAI I NENGAH ARTHA I NYOMAN GEDE ASTAWA I Nyoman Rai I NYOMAN RAI I NYOMAN SUTEDJA I NYOMAN WIJAYA I NYOMAN YOGI SUPARTHA I PUTU GEDE ARDHANA I PUTU PANCA DIANTARA PUTRA I PUTU SUDANA I Wayan Diara I WAYAN GEDE WIRYANTARA I WAYAN SUAMBA I Wayan Suarna I Wayan Suarna I WAYAN WIRAATMAJA IDA AYU MAYUN Ida Ayu Putri Darmawati Ida Ayu Putri Darmawati Ida Ayu Putri Darmawati Ida Ayu Putri Darmawati Ida Ayu IDA PEREIRA DOS SANTOS IN RAI ING ASTAWA IVAN MATHEUS NAINGGOLAN Jaya Duarsa Mintarajasa KETUT KARTHA DINATA Ketut Weda Setora Khamdan Khalimi KOMANG INTAN CAHYANI Melvin Kala opung Ngurah Gede Astawa NI KADEK EMA SUSTIA DEWI Ni Komang Alit Astiari Ni Luh Kartini NI LUH MADE PRADNYAWATHI NI MADE TRIGUNASIH Ni Nyoman Ari Maya Dewi NI NYOMAN ARI MAYADEWI Ni Nyoman Suryantini NI NYOMAN SURYANTINI NI PUTU EKA APRIASTUTI NI WAYAN SUNITI NURITA TORUAN-MATHIUS PAULA TIBURSIANA LOLI TENTI PUTU SUGITA Rindang Dwiyani ROYAN PRACAHYO S.A.N. ARYAWATI SALBERD FERDINAN RONDO Sudirman Yahya UTAMA, I WAYAN EKA KARYA UTAMI UTAMI WIDHIANTHINI WIDHIANTHINI Wiraatmaja, Wayan YOSEPH SITIO YUDHA, I KADEK WISMA YUDHANI WIDHYA HARTIWI Yuyun Fitriani Yuyun Fitriani