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All Journal Jurnal Penelitian Saintek Inersia : Jurnal Teknik Sipil dan Arsitektur ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi Prosiding Seminar Biologi Jurnal Akademika Biologi Teknosains: Media Informasi Sains dan Teknologi Journal of Biomedicine and Translational Research Prosiding KPSDA Tesa Arsitektur Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA BIOMA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi NICHE Journal of Tropical Biology Jurnal PROTEKSI (Proyeksi Teknik Sipil) Jurnal Teknika : Jurnal Teoritis dan Terapan Bidang Keteknikan Proceeding Biology Education Conference Berkala Penelitian Hayati Greensphere: Journal of Environmental Chemistry Makara Journal of Science JURNAL BIOLOGI PAPUA AGRIC
Wijanarka Wijanarka
Departement Of Biology, Faculty Of Science And Mathematics, The University Of Diponegoro, Semarang Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences

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Journal : Makara Journal of Science

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Intergenus Protoplast Fusion between Pichia manshurica and Rhodosporidium paludigenum to Increase the Production of Inulinase Wijanarka, Wijanarka; Soetarto, Endang Sutariningsih; Dewi, Kumala; Indrianto, Ari
Makara Journal of Science Vol. 18, No. 4
Publisher : UI Scholars Hub

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Abstract

The purposes of this study was to identify the optimum concentration of the lytic enzyme Glucanex for protoplast isolation and to conduct fusion for the purpose of increasing inulinase production. The study performs the protoplast fusion technique using Pichia manshurica and Rhodosporidium paludigenum. Protoplast fusion consists of a series of stages: protoplast isolation, protoplast fusion, protoplast regeneration, and analysis of hybrid fusion results. Protoplast isolation and fusion success rate are determined by various factors, including age of the culture, media type, and type of lytic enzymes used. Hybrid results were analyzed using a fungicide as a marker and measuring specific growth rate (µ) of the hybrid compared with parental growth rates. Results demonstrated that a concentration of 4 mg/mL of Glucanex produces the greatest number of protoplasts, 7.2 x 1010 (cell/mL) for P. manshurica and 8.8 x 1010 (cell/mL) for Rh. paludigenum. The results of analysis of hybrid fusions indicate that the study has identified a new fusant, called fusant F4. Fusant F4 is capable of producing the highest inulinase, 0.6892 IU, compared with parentals P. manshurica, 0557 IU, and Rh. paludigenum, 0.3263 IU. Fusant F4 has specific growth rate (µ) of 0.3360/h and generation time (g) of 2.0629 h.
Co-Authors Agung Suprihadi Aini, Riris Nur Anggoro, Naufal Sebastian Anto Budiharjo Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Arina Tri Lunggani Asy'ari, Mukhammad Ayuningtyas, Annisa Nur Azzahra, Meilidya Falkhiya Chatarina Umbul Wahyuni Dewi Ayu Maryati Doktorasaintifika, Heradita Kaniaazzahra Dyah Wulandari Endang Kusdiyantini Endang Kusdiyantini Endang Kusdiyantini Endang Sutariningsih Endang Sutariningsih ENDANG SUTARININGSIH SOETARTO Endang Sutariningsih Soetarto Endang Sutariningsih Soetarto Fathika Fitrania Fauzaan, Muhammad Faishal Fitri Ulfana Risky Fransenda, Auxensius Rexer Fransisco, Theo Ginting, Ledy HARTAJANIE, LAKSMI Herida, Azalia Puspa Hermin Hermin Hermin Pancasakti Kusumaningrum Ihdina Isfara Suteja Ika Anggraini Indra Bakti Sangalang Inggrit Amedia, Inggrit K Endang Kumala Dewi Kumala Dewi Kumala Dewi Kumala Dewi Lailatul Mubarokhah Laily Kurniawati Lindayani, Lindayani Lynda - Sutrisna Maria Sarah Fadillah Maulana, Anand Reyna Maulana, Muhammad Iqbal Maulida Aglinia Mauritz Nicolaas Wattimena Mawarni, Risa Arum MG Isworo Rukmi Moch Ali Utomo Mochamad Hadi Muhammad Nur Muhammad Zainuri NANIK RAHMANI Nomeritae Nomeritae Noor Hamidah Nurhayati Nurhayati Pragati Wira Anggini Pratama Jujur Wibawa Putri S. Noor Rahmasari, Dianti Rahmasari, Dianti Rahmawati Dewi Rejeki Siti Ferniah Romario Dion Rudi Waluyo salamah salamah Sarjana Parman Sarsa A.N Shabrina, Jauhara Siti Maisaroh Siti Nur Jannah Sri Pujiyanto Sulis Setyowati, Sulis Susiana Purwantisari T. A. Lestari Tia Erfianti Tony Hadibarata, Tony Wahyuningsih, Candra YOPI YOPI Yusuf, Hadistiyani