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All Journal Jurnal Penelitian Saintek Inersia : Jurnal Teknik Sipil dan Arsitektur ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi Prosiding Seminar Biologi Jurnal Akademika Biologi Teknosains: Media Informasi Sains dan Teknologi Journal of Biomedicine and Translational Research Prosiding KPSDA Tesa Arsitektur Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA BIOMA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi NICHE Journal of Tropical Biology Jurnal PROTEKSI (Proyeksi Teknik Sipil) Jurnal Teknika : Jurnal Teoritis dan Terapan Bidang Keteknikan Proceeding Biology Education Conference Berkala Penelitian Hayati Greensphere: Journal of Environmental Chemistry Makara Journal of Science JURNAL BIOLOGI PAPUA AGRIC
Wijanarka Wijanarka
Departement Of Biology, Faculty Of Science And Mathematics, The University Of Diponegoro, Semarang Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences

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Journal : Prosiding KPSDA

 1 
Biolitik Enzim Bekicot (Achatina fulica) Sebagai Agen Fusi Protoplas Pichia manshurica Intraspecifik Wijanarka, Wijanarka
Prosiding KPSDA Vol 1, No 1 (2015): Prosiding KPSDA 2015
Publisher : Prosiding KPSDA

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Abstract

Enzymes were an important role both in the food or beverage industry, but there is also an enzyme that acts as a breaker of the organism or cell wall of microorganisms. The enzyme known as biolytic enzyme. Extraction enzyme taken from the snail (Achatina fulica), especially in the abdomen, as in this section contains compounds glukorononidase β, and beta-glucanase and endo arylsulphatase (Ezeronye and Okerentugba, 2001). This enzyme was an important role before the fusion process takes place, namely for protoplast isolation. Furthermore, the enzyme used to break down the cell wall of the yeast Pichia manshurica. The yeasts are indigenus located around dahlia tubers and has the ability to produce inulinase (EC 3.2.1.7). Protoplast fusion is one way to improve the strain, so hopefully we will get a new strain and superior compared to its parent. The purpose of this research was using enzymes from snail to break the cell walls of yeast (protoplast isolation) and to obtain new fusan. Protoplast isolation performed enzymatically by using enzyme biolytic  of snail. Fusion process was done by mixing the two parental pellets in solution osmotic stabilizer sorbitol solution of 1.0 mol / L containing 30% PEG 6000 (polyethylene glycol) and 10 mM CaCl2 for 20 minutes. The results showed that the concentration of biolytic 100% can be utilized as an agent biolytic enzyme and capable of producing protoplasts of 8.1 x 109 and it has been found that 3 fusan  namely were Fusan D F1, F4 and F7 D.
Co-Authors Agung Suprihadi Aini, Riris Nur Anggoro, Naufal Sebastian Anto Budiharjo Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Arina Tri Lunggani Asy'ari, Mukhammad Ayuningtyas, Annisa Nur Azzahra, Meilidya Falkhiya Chatarina Umbul Wahyuni Dewi Ayu Maryati Doktorasaintifika, Heradita Kaniaazzahra Dyah Wulandari Endang Kusdiyantini Endang Kusdiyantini Endang Kusdiyantini Endang Sutariningsih Endang Sutariningsih ENDANG SUTARININGSIH SOETARTO Endang Sutariningsih Soetarto Endang Sutariningsih Soetarto Fathika Fitrania Fauzaan, Muhammad Faishal Fitri Ulfana Risky Fransenda, Auxensius Rexer Fransisco, Theo Ginting, Ledy HARTAJANIE, LAKSMI Herida, Azalia Puspa Hermin Hermin Hermin Pancasakti Kusumaningrum Ihdina Isfara Suteja Ika Anggraini Indra Bakti Sangalang Inggrit Amedia, Inggrit K Endang Kumala Dewi Kumala Dewi Kumala Dewi Kumala Dewi Lailatul Mubarokhah Laily Kurniawati Lindayani, Lindayani Lynda - Sutrisna Maria Sarah Fadillah Maulana, Anand Reyna Maulana, Muhammad Iqbal Maulida Aglinia Mauritz Nicolaas Wattimena Mawarni, Risa Arum MG Isworo Rukmi Moch Ali Utomo Mochamad Hadi Muhammad Nur Muhammad Zainuri NANIK RAHMANI Nomeritae Nomeritae Noor Hamidah Nurhayati Nurhayati Pragati Wira Anggini Pratama Jujur Wibawa Putri S. Noor Rahmasari, Dianti Rahmasari, Dianti Rahmawati Dewi Rejeki Siti Ferniah Romario Dion Rudi Waluyo salamah salamah Sarjana Parman Sarsa A.N Shabrina, Jauhara Siti Maisaroh Siti Nur Jannah Sri Pujiyanto Sulis Setyowati, Sulis Susiana Purwantisari T. A. Lestari Tia Erfianti Tony Hadibarata, Tony Wahyuningsih, Candra YOPI YOPI Yusuf, Hadistiyani