Article Per Year (5 Year)
p-Index From 2021 - 2026
2.036
P-Index
This Author published in this journals
All Journal Jurnal Penelitian Saintek Inersia : Jurnal Teknik Sipil dan Arsitektur ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi Prosiding Seminar Biologi Jurnal Akademika Biologi Teknosains: Media Informasi Sains dan Teknologi Journal of Biomedicine and Translational Research Prosiding KPSDA Tesa Arsitektur Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA BIOMA Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Berkala Bioteknologi NICHE Journal of Tropical Biology Jurnal PROTEKSI (Proyeksi Teknik Sipil) Jurnal Teknika : Jurnal Teoritis dan Terapan Bidang Keteknikan Proceeding Biology Education Conference Berkala Penelitian Hayati Greensphere: Journal of Environmental Chemistry Makara Journal of Science JURNAL BIOLOGI PAPUA AGRIC
Wijanarka Wijanarka
Departement Of Biology, Faculty Of Science And Mathematics, The University Of Diponegoro, Semarang Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences

Published : 44 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : JURNAL BIOLOGI INDONESIA

 1 
Pemurnian Parsial dan Karakterisasi Amilase dari Bakteri Laut Arthrobacter arilaitensis LBF-003 Rahmasari, Dianti; Wijanarka, Wijanarka; Pujiyanto, Sri; Rahmani, Nanik; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2323

Abstract

Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC 3.2.1.1), which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and it’s purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.Keywords: alfa amylase, marine bacterium, Arthrobacter arilaitensis, purification, charaterization
PEMURNIAN PARSIAL DAN KARAKTERISASI AMILASE DARI BAKTERI LAUT ARTHROBACTER ARILAITENSIS LBF-003 Rahmasari, Dianti; Wijanarka, Wijanarka; Pujiyanto, Sri; Rahmani, Nanik; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 1 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i1.2323

Abstract

Starch is an abundant carbon source in nature, and ?-amylase (1, 4-?-D-glucanohydrolase; EC 3.2.1.1), which hydrolyzes ?-1, 4-glucosidic linkage in starch-related molecules. Microbe ?-amylase production is a hydrolytic enzyme and one ofinterest in its microbial production has increased dramatically due to its wide spread use in food, textile, baking anddetergent industries in recent years. Here we report ?-amylase from marine bacterium which was purified andcharacterized, as well as analyzed its hydrolysis product on starch. The enzyme of Arthrobacter arilaitensis partiallypurified by acetone precipitation with 90% and ion exchange chromatography produced specific activity 0.25 U/mg and0.38 U/mg, and it?s purity rate increased until 1.14 fold compared with former crude extract. Purifed extracelluler amilasehad an optimum activity at temperature 50°C and pH 9.0. An apparent molecular mass was between 50-75 kDa, asestimated by zimogram electrophoresis. Hydrolysis products of this enzyme on starch were maltose, maltotriose andmaltoheptaose.Keywords: alfa amylase, marine bacterium, Arthrobacter arilaitensis, purification, charaterization
Co-Authors Agung Suprihadi Aini, Riris Nur Anggoro, Naufal Sebastian Anto Budiharjo Ari Indrianto Ari Indrianto Ari Indrianto Ari Indrianto Arina Tri Lunggani Asy'ari, Mukhammad Ayuningtyas, Annisa Nur Azzahra, Meilidya Falkhiya Chatarina Umbul Wahyuni Dewi Ayu Maryati Doktorasaintifika, Heradita Kaniaazzahra Dyah Wulandari Endang Kusdiyantini Endang Kusdiyantini Endang Kusdiyantini Endang Sutariningsih Endang Sutariningsih ENDANG SUTARININGSIH SOETARTO Endang Sutariningsih Soetarto Endang Sutariningsih Soetarto Fathika Fitrania Fauzaan, Muhammad Faishal Fitri Ulfana Risky Fransenda, Auxensius Rexer Fransisco, Theo Ginting, Ledy HARTAJANIE, LAKSMI Herida, Azalia Puspa Hermin Hermin Hermin Pancasakti Kusumaningrum Ihdina Isfara Suteja Ika Anggraini Indra Bakti Sangalang Inggrit Amedia, Inggrit K Endang Kumala Dewi Kumala Dewi Kumala Dewi Kumala Dewi Lailatul Mubarokhah Laily Kurniawati Lindayani, Lindayani Lynda - Sutrisna Maria Sarah Fadillah Maulana, Anand Reyna Maulana, Muhammad Iqbal Maulida Aglinia Mauritz Nicolaas Wattimena Mawarni, Risa Arum MG Isworo Rukmi Moch Ali Utomo Mochamad Hadi Muhammad Nur Muhammad Zainuri NANIK RAHMANI Nomeritae Nomeritae Noor Hamidah Nurhayati Nurhayati Pragati Wira Anggini Pratama Jujur Wibawa Putri S. Noor Rahmasari, Dianti Rahmasari, Dianti Rahmawati Dewi Rejeki Siti Ferniah Romario Dion Rudi Waluyo salamah salamah Sarjana Parman Sarsa A.N Shabrina, Jauhara Siti Maisaroh Siti Nur Jannah Sri Pujiyanto Sulis Setyowati, Sulis Susiana Purwantisari T. A. Lestari Tia Erfianti Tony Hadibarata, Tony Wahyuningsih, Candra YOPI YOPI Yusuf, Hadistiyani