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Growth of typha grass (Typha angustifolia) on gold-mine tailings with application of arbuscular mycorrhiza fungi LULUK SETYANINGSIH; ARUM SEKAR WULANDARI; HAMIM HAMIM
Biodiversitas Journal of Biological Diversity Vol. 19 No. 2 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190218

Abstract

Setyaningsih L, Wulandari AS, Hamim H. 2018. Growth of typha grass (Typha angustifolia) on gold-mine tailings with application of arbuscular mycorrhiza fungi. Biodiversitas 19: 504-509. Gold mine tailings contain extreme physical and chemical properties, which inhibit plant growth due to lower nutrition and higher heavy metal contaminants. Typha (Typha angustifolia) is type of grass growing well on waterlogged area including tailing dam. The objective of this study was to investigate the effect of arbuscular mycorrhizal fungi (AMF) in combination with compost and soil on the typha growth in gold mine tailings. The study was conducted in greenhouse by inoculating two AMF isolates (Glomus etunicatum and G. manihotis) to typha seedlings grown in pure tailing media, mixed tailing-compost media, and mixed tailing-compost-soil media. The compatibility and growth of typha grass were analysed after 1 month. Results showed that G. etunicatum and G. manihotis application significantly increased AMF colonization of typha roots up to 16.6% and 21.8% respectively. The length, number of leaves and biomass of typha also increased up to 90%, 50% and 97% respectively compared to those without AMF inoculation. G. etunicatum contributed the best growth of typha grown in mixed compost-soil-tailings, resulting in double increase of its length and biomass. The application of G. manihotis did not significantly increase the growth of typha in mixed media; however, under pure tailing, this mycorrhiza had the best induction for typha biomass and leaf number. In general, AMF application increased growth of typha grass in tailings media.
Morphology and anatomy of the fruit and seed of Cananga odorata (Lam.) Hook.f. & Thomson Ferawati Oktia Nurhayani; ARUM SEKAR WULANDARI; TATIEK KARTIKA SUHARSI
Biodiversitas Journal of Biological Diversity Vol. 20 No. 11 (2019)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d201112

Abstract

Abstract. Nurhayani FO, Wulandari AS, Suharsi TK. 2019. Morphology and anatomy of the fruit and seed of Cananga odorata (Lam.) Hook.f. & Thomson. Biodiversitas 20: 3199-3206. Cananga odorata (Lam.) Hook.f. & Thomson is one of the Annonaceae family which has high benefits for humans such as constructions, furniture, cosmetics, perfumes, and traditional medicines. The planting efforts were needed to support the existence of these plants. These plants have low regeneration and germination. The morphology and anatomy of fruit and seed could enhance the understanding of the reproductive biology in the species. There is little information about the morphology and anatomy of fruit and seed of C. odorata var. odorata forma genuina. The aim of this study is to understand the morphology and anatomy of fruit and seed of C. odorata. The morphologic description was made from the analysis of the fresh fruits and seeds. The anatomical description was made from the analysis of permanent slides with longitudinal sections of the fruits and seeds. The samples were fixed, dehydrated, alcoholized, embedding in paraffin, and stained with safranin-fast green. Data analysis consists of the morphological observation (shape, color, size and the number of seeds per fruits) and the anatomical observation (the pericarp layers of fruit, the seed coat layers, and the structure in the seed). The fruit of C. odorata var. odorata forma genuina was fleshy, ovoid or obovoid, 0.98-2.75 cm long, 0.42-1.63 cm in diameter, and contain 1-12 seeds. The seeds were pale brown, flattened, ovoid, and hard. The fruit anatomy showed that the pericarp layers derived from parenchymatous cells and differentiated into three zones, i.e. exocarp, mesocarp, and endocarp. The seed has ruminated endosperm with irregular folds. The seed coat was divided into three integuments, i.e. outer, middle, and inner integument. The information about the fruit and seed anatomy of C. odorata var. odorata forma genuina might enhance the understanding of future studies regarding natural reproduction and conservation programs with generative propagation.
Arbuscular mycorrhizal fungi and Rhizobium enhance the growth of Samanea saman (trembesi) planted on gold-mine tailings in Pongkor, West Java, Indonesia Luluk Setyaningsih; FIRLI AZHAR DIKDAYATAMA; ARUM SEKAR WULANDARI
Biodiversitas Journal of Biological Diversity Vol. 21 No. 2 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210224

Abstract

Abstract. Setyaningsih L, Dikdayatama FA, Wulandari AS. 2020. Arbuscular mycorrhizal fungi and Rhizobium enhance the growth of Samanea saman (trembesi) planted on gold-mine tailings in Pongkor, West Java, Indonesia. Biodiversitas 21: 611-616. Revegetation of severely degraded lands, such as gold-mine tailings, requires comprehensive approach including the selection of appropriate tree species and the improvement of soil fertility with the application of microorganisms. This study aimed to analyze the growth of trembesi (Samanea saman (Jacq.) Merr) seedlings inoculated with Arbuscular Mycorrhizal Fungi (AMF) and rhizobial bacteria (RB) on gold-mine tailings in Pongkor, Bogor, West Java, Indonesia. The AMF, RB, and mixture of AMF and RB were inoculated into S. saman seedlings and then the seedlings were grown on gold mine tailings land. Seedling growth, AMF infection and RB nodulation were observed after 12 weeks after planting. The results showed that the treatments facilitated AMF colonization by up to 20.7% and RB nodulation up to 22 nodules per plant. The inoculation of S. saman seedlings with AMF and RB significantly increased the growth on diameter, biomass, and root length, with values of root-to-shoot ratio of 3.1-4.3. The roots of S. saman seedlings were also able to penetrate the depth of the tailings solum. These results showed that AMF and RB application effectively increased the growth of S. saman seedlings in the gold tailings field.
The growth of sengon (Parasarienthes falcataria) and citronella (Cymbopogon nardus) productivity performance in agroforestry system HANIFA RAHMAH; NURHENI WIJAYANTO; ARUM SEKAR WULANDARI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 6 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240603

Abstract

Abstract. Rahmah H, Wijayanto N, Wulandari AS. 2023. The growth of sengon (Parasarienthes falcataria) and citronella (Cymbopogon nardus) productivity performance in agroforestry system. Biodiversitas 24: 3114-3119. Sengon is a fast-growing plant species widely planted in community forests. Sengon is widely cultivated in agroforestry systems with seasonal crops. The main obstacle to cultivation in agroforestry systems is low growth due to competition for nutrients and low absorption of sunlight. This study aims to analyze the growth of sengon varieties and citronella varieties. The research was conducted for six months in the Cikabayan field, Faculty of Forestry, IPB University, Bogor, West Java. This study consisted of two activities: (i) analyzing the effect of the two-year-old sengon variety on the growth of tree height, diameter, crown, and volume, (ii) analyzing the effect of the citronella variety on the growth and yield of citronella oil. The experiment was designed with three factors, a completely randomized design with three replications. The results showed that Sengon Solomon had higher growth than Sengon Kendal. Sengon Solomon F2 had better growth than Sengon Solomon F1 and Kendal locale. Citronella plants grown under the local stands of Sengon Kendal had the best productivity. Lemongrass variety G1 had better growth than the Sitrona 2 Agribun variety in the shaded condition of sengon stands. However, the Sitrona 2 Agribun variety's yield has a higher value than the G1 citronella variety.
Effect of Azadirachta excelsa and Melia azedarach extracts on soybean germination RUMMI AZAHRA GUMILAR; NURHENI WIJAYANTO; ARUM SEKAR WULANDARI
Nusantara Bioscience Vol. 9 No. 4 (2017)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n090402

Abstract

Gumilar RA, Wijayanto N, Wulandari AS. 2017. Effect of Azadirachta excelsa and Melia azedarach extracts on soybean germination. Nusantara Bioscience 9: 346-351. Azadirachta excelsa and Melia azedarach are potential tree species developed in agroforestry system. Both plants are fast growing species and good for timber use. However, there is an inadequacy on both species due to their allelopathic compound contents. Allelopathic compounds in the plants are distributed from root, stem, leaf, and fruit; which at certain concentrations could inhibit germination, growth, and development of other plants. The objective of this study was to analyze the effects of root, leaf, and twig extracts of M. azedarach and A. excelsa on the germination of soybean. Result of this study showed that the root, leaf, and twig extracts of M. azedarach and A. excelsa were able to inhibit the development of soybean. 5 % twig extract of M. azedarach significantly inhibited the germination by 77.75%. Leaf and twig extracts of A. excelsa at 1.25% concentration has shown to provide stimulant effect on the germination of soybean.
Effects of Gamma Ray Irradiation to Induce Genetic Variability of Teak Planlets (Tectona grandis Linn. F.) Ahmad Parlaongan; Supriyanto; Arum Sekar Wulandari
Journal of Sylva Indonesiana Vol. 5 No. 01 (2022): Journal of Sylva Indonesiana
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (923.103 KB) | DOI: 10.32734/jsi.v5i01.6166

Abstract

Teak planlets (Tectona grandis) of Salomon clones were irradiated by gamma rays to induce genetic variability for growth improvement. The objectives of this research were to analyze the radiosensitivity of T. grandis Salomon clones by gamma ray irradiation and to analyze the genetic variations using Random Amplified Polymorphic Deoxyribonucleic Acid (RAPD). For those purposes, teak planlets were irradiated using gamma rays at 5 different dosages, those were 0 Gy, 10 Gy, 20 Gy, 30 Gy, and 40 Gy. Lethal doses50 (LD50) and reduced doses50 (RD50) were obtained using Curve-fit Analysis. Furthermore, the irradiated planlets were subcultured on medium MS + 0.1 kinetin (M1V0) the surviving M1V0 plantlets were multiplied to MS + 0.1 kinetin, so it is obtained the M1V1 generation. Genetic variation of the mutant was molecularly analyzed using RAPD methods and the variability was calculated using Analysis of Molecular Variance (AMOVA). Results of this research showed that lethal doses50 (LD50) and reduced doses50 (RD50) values were obtained at 24.5 Gy and 7.85 Gy, respectively. It includes genomes of small size and a small number of genomes. The genetic variation of mutant individuals in among and within the treatment of gamma irradiation was 16% and 84% according to AMOVA. It means that the dominant effect of genetic material caused genetic variation in mutant M1V1 generations. This dominance of genetic material could be caused by the response combination of tissue culture treatment and genetic factors that had the potency to be used as materials to select desired clones in the next stage.
Co-Authors . Supriyanto Abdonia W. Finmeta Aditya Wardani Afrida Rizka Farzana Ahmad Parlaongan Al Muzahiq, Hizbullah Alwysihah, Hafid Anindita Julian ARIFA MULYESTHI RAHMAWATHI Atok Subiakto Basuki Wasis Benny Subandy Benny Subandy Cahyo Wibowo Chaerul H, Rachmad Dadan Mulyana Dela Meisuda Mar’Atussholihah Derie Kusuma Budi Ningrum Dewa Made Juli Santika Dian Kirani, Annisa Edhi Sandra Erina Sulistiani Esthi Liani Agustiani Fadhlurrahman, Muhammad Miftah Farikhah, Anisatul Fatimah Nur Istiqomah Fauziah, Nadira Aribah Febrian , Febrian Ari Nugroho Febrian Ari Nugroho Febrina Keumala, Cut Ferawati Oktia Nurhayani Ferawati Oktia Nurhayani FIRLI AZHAR DIKDAYATAMA Ganis Citra Purmadewi H, Rachmad Chaerul Hamim Hamim Handayani, Triaty HANIFA RAHMAH Hannum Wulan Febrianingrum Hariadi Propantoko Helga Sugiarti Ida Rosita Ika Roostika Irdika Mansur Islami, Naluri Siyasah Istikorini, Yunik LULUK SETYANINGSIH Melya Riniarti Muhammad Ridwan Neo Endra Lelana, Neo Endra Nurhayani, Ferawati Oktia NURHENI WIJAYANTO Nurheni Wijayanto NURHENI WIJAYANTO NURHENI WIJAYANTO Pertiwi, Hutami Indah Puspitasari, Sri Rakhman , Rizali Ratna Damayanti Sianturi Ratna Uli Damayanti Sianturi Revi Novan Rhomi Ardiansyah Rifqie Mardiansyah Purmadi Rizki Adisetia RUMMI AZAHRA GUMILAR Rummi Azahra Gumilar Sabar Sampulan Nasution Siregar , Elisa Weber Siska Noer Avifah Siti Jaenab Sri Susanti Sri Wilarso B. Sri Wilarso Budi Suci Ratna Puri Supriyanto Supriyanto . Supriyanto Supriyanto Suwondo Suwondo Suyogia Nur Azis TATIEK KARTIKA SUHARSI Tofan Randy Wijaya Wahyu Krisna Yuli Fitriani Yus Septiawati Yusuf Sigit Ahmad Fauzan