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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Program Kreativitas Mahasiswa - Penelitian Journal of Islamic Pharmacy Science and Technology Indonesia Majalah Kesehatan FKUB Jurnal Penelitian dan Pengabdian Kepada Masyarakat UNSIQ Darmabakti : Junal Pengabdian dan Pemberdayaan Masyarakat Caring : Jurnal Pengabdian Masyarakat Tri Dharma Mandiri: Diseminasi dan Hilirisasi Riset Kepada Masyarakat (Jurnal Pengabdian Kepada Masyarakat) Jurnal Bioteknologi & Biosains Indonesia
VALENTINA YURINA
Program Studi Farmasi, Fakultas Kedokteran Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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OPTIMIZATION OF INTRANASAL COVID-19 VACCINE FORMULATION WITH Lactococcus lactis pNZ HCR BACTERIA AS VECTOR IN LIQUID AEROSOL PREPARATION Yurina, Valentina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.1742

Abstract

Vaccination is an effective method to suppress COVID-19 transmission, but injection-based vaccination is less effective due to its inability to induce mucosal immunity. This study aimed to determine the effects of vaccine formulations on bacteria viability and antigen expression to find the optimal formulation. Three intranasal preparation formulations (F1, F2, and F3) were created with different ingredient compositions, along with a control. Physicochemical tests were conducted on day 0 and day 14 to assess bacterial viability, and antigen expression was evaluated using the western blot method. Formula 2, containing sodium alginate (0.615%), trehalose (4.125%), polyvinyl alcohol (0.1%), and calcium chloride (5%), exhibited the best viability test results, although no significant differences were observed among the groups. The study concluded that variations in composition concentrations could affect bacterial stability, with Formula 2 showing the best results in terms of bacteria viability and antigen expression up to 14 days after formulation.
Oral Immunization with Recombinant Lactococcus lactis and Retinoic Acid Boost Immune Response in Mice Nugroho, Inggil Cahyo; Amalia, Maulida Nisa; Juniardi, Muhamad Rizki; Marhendra, Eunike Berliana; Yanma, Nadifa Almira; Adianingsih, Oktavia Rahayu; Winarsih, Sri; Widodo, Nashi; Yurina, Valentina
Science and Technology Indonesia Vol. 11 No. 1 (2026): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2026.11.1.55-64

Abstract

The COVID-19 vaccine is one of the most important approaches for preventing SARS-CoV-2 virus transmission and spread. Oral vaccines are a promising option for preventing SARS-CoV-2 infection because it can activate both the mucosal and cellular immune systems. Previous research has shown that administering oral and intranasal vaccines can induce an immune response in mice. The effectiveness of the SARS-CoV-2 oral vaccine was evaluated in this study by combining spike protein with a carrier of food-grade recombinant Lactococcus lactis bacteria and a retinoic acid adjuvant. Mice were divided into three groups: a negative control (no treatment), a positive control (L. lactis recombinant), and a third group (L. lactis recombinant plus 300 μg retinoic acid). The vaccines were given three times, with a three-week interval between each. The serum levels of IgG, IgA, and IgE were determined using the ELISA method at the end of the study. CD4 and CD8 cellswere detected using immunofluorescence. While not statistically significant, the results showed that the retinoic acid groups had the highest anti-spike antibody levels of the three groups. In comparison to the control group, CD4 and CD8 cells increased in the spleens of mice given retinoic acid. There was no difference in temperature or IgE levels between vaccinated and non-vaccinated mice, indicating that the vaccine caused no allergic reaction. This study’s findings suggest that retinoic acid adjuvant can stimulate the cellular and humoral immune system.
Anti-Photoaging Effects of Moisturizer and Serum Containing Andrographis paniculata, Syzygium aromaticum, and Pogostemon cablin: A Network Pharmacology, Molecular Docking, and in vivo Study Adianingsih, Oktavia Rahayu; Johan, Christopher Kuncoro; Widarson; Ravsanjani, Eurica Andriani Najwa; Sutiyono, Bershiella Amandari; Qurrotuain, Iffah; Yurina, Valentina; Puspita, Oktavia Eka; Widasmara, Dhelya; Saitama, Akbar; Hariyono, Didik
Science and Technology Indonesia Vol. 11 No. 1 (2026): January
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2026.11.1.19-34

Abstract

Ultraviolet-B (UVB)-induced photoaging accelerates collagen degradation, disrupts dermal structure, and compromises epidermal integrity. Natural extracts, such as Andrographis paniculata, Syzygium aromaticum, and Pogostemon cablin demonstrated antioxidant and anti-aging properties to mitigate these damages. This study investigated the protective effects of serum and moisturizer containing A. paniculata extract, S. aromaticum oil, and P. cablin oil on UVB-induced photoaging rats, by integrating network pharmacology and in vivo experiment. Compounds and photoaging-associated targets were retrieved through online databases. Enrichment analyses were performed using Metascape. Protein-protein interaction (PPI) construction and herbs-compounds-targets-pathways disease network were performed using STRING and Cytoscape 3.10.0, respectively, followed by molecular docking. An in vivo study was conducted to evaluate epidermal thickness, collagen density, and dermo-epidermal junction (DEJ) structure. Eleven key phytoconstituents and twelve core targets were identified in anti-photoaging molecular mechanisms using network pharmacology. Molecular docking analysis revealed that 7-O-methylwogonin demonstrated favorable binding against ten photoaging-associated targets. KEGG enrichment analysis showed potential inflammation processes inhibition, including photocarcinogenesis, AGE-RAGE signaling pathway, and TNF-α expression. Furthermore, treatment with serum, moisturizer, or combination of both significantly reduced epidermal thickening and enhanced collagen density compared to the UVB-exposed only group. Although no significant difference was observed in DEJ interdigitation index across groups, treated groups exhibited a trend towards better preservation of DEJ structure relative to the UVB group. In summary, serum and moisturizer containing A. paniculata, S. aromaticum, and P. cablin extracts demonstrated potential anti-photoaging effects by reducing epidermal thickness and preserving collagen density in UVB-exposed rats. These findings support these botanical-based formulations potential as effective anti-aging skincare.
PENDEKATAN BIOINFORMATIKA DALAM STUDI EPITOP PADA S2 PROTEIN SPIKE SARS-CoV-2 DENGAN VARIASI ADJUVAN Fikria Amalia Madani, Jadida; Rahayu Adianingsih, Oktavia; Yurina, Valentina
Majalah Kesehatan Vol. 0 No. 00 (2026): Article in Press
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/

Abstract

Pendekatan bioinformatika saat ini banyak digunakan dalam pengembangan vaksin, terutama terhadap patogen yang mudah bermutasi seperti SARS-CoV-2. Salah satu strategi yang dikembangkan adalah vaksin berbasis epitop dengan tambahan adjuvan untuk meningkatkan respons imun. Penelitian ini bertujuan untuk membandingkan efektivitas tiga adjuvan, yaitu 50S ribosomal protein L7/L12 (rpLL), β-defensin, dan cholera toxin subunit B (CTB) dalam meningkatkan imunogenisitas vaksin berbasis epitop S2 protein spike SARS-CoV-2. Sekuens protein enam varian SARS-CoV-2 (Wuhan, Alpha, Beta, Gamma, Delta, dan Omicron) dan tiga adjuvan diperoleh dari database NCBI. Epitop sel T dan B diprediksi menggunakan IEDB, kemudian diseleksi berdasarkan sifat imunologis (antigenik, nontoksik, nonalergen), nonhomolog, serta cakupan populasi global (83,57%) dengan wilayah tertinggi di Asia Timur (93,62%). Wilayah konservatif subunit S2 berdasarkan epitop terpilih dikonstruksi menjadi tiga desain vaksin, masing-masing dengan satu adjuvan. Validasi struktur menunjukkan semua model berada dalam rentang kualitas yang baik (ERRAT >90%, Ramachandran >90%). Skor docking HADDOCK terbaik diperoleh pada vaksin dengan rpLL terhadap TLR4 (-95,3), vaksin dengan β-defensin terhadap TLR3 (-38,2) dan MHC-II (-133,6), serta vaksin dengan CTB terhadap MHC-I (-128,0). Profil interaksi tiga vaksin dengan masing-masing reseptor menunjukkan bahwa vaksin dengan CTB memiliki ikatan yang lebih kuat dan konsisten pada sebagian besar reseptor. Simulasi sistem imun melalui C-IMMSIM menunjukkan desain ini menghasilkan kadar IgG dan IgM tertinggi serta aktivasi limfosit T CD4+, CD8+, dan limfosit B yang lebih baik. Hasil ini menunjukkan bahwa CTB merupakan adjuvan paling potensial dalam meningkatkan imunogenisitas vaksin. Penelitian ini mendukung pendekatan in silico dalam pengembangan vaksin yang efisien dan pemilihan adjuvan yang lebih optimal.
Co-Authors Achmad Rudijanto Alvan Febrian Shalas Amalia, Maulida Nisa Anggita Rosiana Putri Anggita Rosiana Putri Angi Nurkhairina, Angi Anita Puspa Widiyana Ardina Pramesti Putri, Ardina Pramesti Bachtiar Rifai Pratita Ihsan Bachtiar Rifai Pratita Ihsan Dhelya Widasmara Diah Royani Meisani Didik Hariyono Ema Pristi Yunita, Ema Pristi Eva Putri Arfiani Fikria Amalia Madani, Jadida Fredo Tamara, Fredo Ihsan, Bachtiar Rifai Pratita Johan, Christopher Kuncoro Juniardi, Muhamad Rizki Kesuma, Suryanata Kusworini Handono Luthfi Ahmad Muchlashi Marhendra, Eunike Berliana Megasari, Suci Muchlashi, Luthfi Ahmad Nabila Ayu Safitri, Nabila Ayu Nashi Widodo Nugroho, Inggil Cahyo Oktavia Eka Puspita Oktavia Eka Puspita, Oktavia Eka Oktavia Rahayu A, Oktavia Rahayu Oktavia Rahayu Adianingsih Poejiani, Soeyati Qurrotuain, Iffah Rahayu Adianingsih, Oktavia Ravsanjani, Eurica Andriani Najwa Saitama, Akbar Sri Winarsih Sutiyono, Bershiella Amandari Tamara Gusti Ebtavanny Thoha Muhajir Albaar, Thoha Muhajir Tri Yudani Mardining Raras Widarson Yanma, Nadifa Almira