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All Journal PROSIDING SEMINAR NASIONAL Jurnal Kesehatan Masyarakat Indonesia JURNAL LITBANG JURNAL KESEHATAN FIKkeS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Biomedika Prosiding Seminar Nasional Unimus
Sri Darmawati
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Journal : PROSIDING SEMINAR NASIONAL

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PROFIL PROTEIN TIGA JENIS DAGING YANG DILUMURI SERBUK BUAH MENGKUDU BERBASIS SDS-PAGE Wa Ode Jariah M; Sri Darmawati
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Noni contains protease enzymes that can hydrolyze proteins by breaking the peptide bond, so it can be used to soften the meat. The purpose of this study was toanalyze protein profile on 3 types of meat (goats, buffalo and cow) before and aftergreased with non-fertilizing powder with 30 minutes immersion. Protein profile of three meat types was analyzed using the SDS-PAGE method. The design of this research is descriptive research with the object of research are goat meat, buffalo and cow greased with powder of noni. The results showed that in control meat (goats, buffalo and cow) that were not greased with noni powder there were many major protein bands compared with minor protein bands. While on goat meat, buffalo and cow greased with powder of noni with concentration 10% b / b, 15% b / b, 20% b / b and 25% b / b showed different results at each concentration that there were many minor protein bands than the major protein bands. The higher concentration powder of Noni then the amount of protein in the meat is more denatured. Based on these results indicate that the protease enzyme contained in the powder of noni is able to break peptide bonds in meat protein to proteinshaped minor bands (micromolecul).Keyword : meat, noni powder, protein profile, SDS-PAGE
PERBANDINGAN EFEK EKSTRAK BUAH ALPUKAT (Persea americana Mill) TERHADAP PERTUMBUHAN BAKTERI Pseudomonas aeruginosa DENGAN METODE DISK DAN SUMURAN Sri Dewi Haryati; Sri Darmawati; Wildiani Wilson
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

This study aimed to analyze the concentration of avocado fruit extracts method disc and wells 10% b/v, 20% b/v, 30% b/v, 40% b/v and 50% b/v in inhibited P.aeruginosasa. The avocado fruit is extracted using the maceration method. Then the activity of avocado fruit to the growth of P.aeruginosa using the method of disc and wells.The results showed the extract of avocado method of disk and wells at concentration10% b/v, 20% b/v, 30% b/v, 40% b/v and 50% b/v can inhibit P.aeruginosa bacteria the mean inhibitory zone of the disc method was 16.6 mm, 21.6 mm, 26.0 mm, 28.4 mm and 29.6 mm, as well as in the method of wells obtained mean inhibition zone respectively that is 25.4 mm, 27.4 mm, 28.8 mm, 30.2 mm and 31.2 mm.The positive control used was ciprofloxacin concentration of 25 μg forming a 33 mm inhibitory zone diameter. One Way ANOVA test was obtained p=0,000  which shows that there is an average difference between the inhibitory zone diameter between concentrations 10% b/v, 20% b/v, 30% b/v, 40% b/v and 50% b/v, as well as there is a marked difference between the disk method and the extraction of avocado extract on the growth of P.aeruginosa.The result shows that the average of the bacterial inhibition zone with the disk method is lower than the well method, the higher the concentration of avocado extract, the higher the inhibitory effect on the growth of P.aeruginosa bacteria. Keywords: Avocado Fruit Extracts, P.aeruginosa, Method Disc, Methode Wells
KARAKTERISASI MOLEKULER PROTEIN FLAGELLIN Salmonella typhi ISOLAT JAWA TENGAH DAN YOGYAKARTA Sri Darmawati; Budi Santosa; Ragil Saptaningtyas
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Bidang MIPA dan Kesehatan The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

The purpose of this study is to molecularly characterize 7 strains of flagellin proteins of Salmonella typhi isolates taken from Central Java (5 strains from Semarang, 1 strain from Salatiga, 1 strain from Magelang) and 2 isolate strains from Yogyakarta (1 strain from Doctor Sarjito Hospital, and 1 strain from Bethesda Hospital). Flagellin gene (fliC) amplification is conducted using PCR (primer LPW 1856 and LPW 1857). Flagellin proteins resulted from Alexan method (2009) is then modified while flagellin protein profiles are obtained with SDS-PAGE method. The results show that the size of flagellin gene (fliC) of S. typhi Isolates from Salatiga and the 8 other strains is respectively 1262bp and 1500bp. Flagellin proteins which are composed of 2-6 protein sub-units consist of 1-2 major proteins and 1-4 minor proteins with the sizes of 16-116 kDa.Keywords: Salmonella typhi, flagellin, Molecular Characterizations
ISOLASI BAKTERI PENGHASIL ENZIM PROTEASE STAPHYLOCOCCUS HOMINIS PADA ONCOM MERAH PASCA FERMENTASI 120 JAM Aulia Harun; Sakti Imam Muchlissin; Ana Hidayati Mukaromah; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Enzymes are complex protein moleculer produced by living cells playing role as catalysts in various chemical processes in the body. Among enzymes playing an important role in human life is protease. The purpose of this study was to determine the presence of protease – producing bacteria found on 120-h post - fermented oncom and to identify the bacteria based on its 16S   rRNA gene analysis. Bacterial isolation and purification was carried out using Nutrient Agar media with spread technique. Of the six bacterial isolates isolated from the oncom sample after 120 hours of fermentation, there was one isolate that had protease activity, namely IROD 5. The protease enzyme income test was carried out using Skim Milk Agar media. Molecular identification process was carried out through sequential analysis of 16S rRNA using PCR method using primers forward F: 5'-AGAGTTGATCCTGGCTCAG-3 'and reverse R: 5'- GGTTACCTTGTTAC. GACTT-3 primers' followed by sequencing process. The protease enzyme production test to bacterial isolate was conducted using Skim Milk Agar. Molecular identification was performed through analysis of 16S rRNA gene sequence using PCR method followed by sequencing process. A single bacterial isolate having proteolytic activity was obtained based on observation of the clear zone of protease surrounding the bacterial colony with a diameter of 72 mm. The 16S rRNA gene sequence of the obtained proteolytic bacterial strain IROD5 has been obtained and analysis on the gene sequence resulted 99% similarity levels with sequence of similar gene s of Staphylococcus hominis. As conclusion, the obtained bacterial isolate in this studyis apotential protease  enzyme  producer  and  molecularly  identified  as  Staphylococcus hominis strains IROD5. Keyword : Protease Enzyme, Gen 16S rRNA, Red Oncom
ANALISIS MOLEKULER PROFIL PROTEIN PILLI UNTUK MENGUNGKAP HUBUNGAN SIMILARITAS 26 STRAIN Salmonella typhi ISOLAT JAWA Sri Darmawati; - Anawar S; W T Artama
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2010: PROSIDING SEMINAR NASIONAL HASIL-HASIL PENELITIAN
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Variasi dan hubungan similaritas 26 strain Salmonella typhi Isolat Jawa merupakan awal untuk melacak protein sub unit pilli spesifik yang memiliki aktivitas hemaglutinasi. Tujuan penelitian analisis molekulerprofil protein pilli untuk mengungkap hubungan similaritas 26 Strain S. typhi Isolat Jawa. Analisis dilakukan terhadap 26 strain yang berasal dari Surabaya, Madiun, Malang, Salatiga, Magelang, Bandung, Bogor, Jakarta, Yogyakarta dengan elektroforesis SDS-PAGE. Analisis hubungan similaritasdigunakan program MVSP, untuk mengkonstruksi dendogram yang mencerminkan klasifikasi dari 26 strain S. typhi Isolat Jawa berdasarkan nilai indeks similaritas (S SM ) dengan algoritma UPGMA. Hasilanalisis profil protein pili menunjukkan (1) Jumlah pita protein sub unit pilli bervariasi : 8-17 pita, BM tertinggi 200 kD, terendah 10 kD, dengan 20 karakter. (2) Protein 100 kD, 50 kD, 45 kD dan 40 kD adalah protein sub unit pilli yang dimiliki oleh 26 strain S. typhi Isolat Jawa. (3) Dari 26 strain S. typhi Isolat Jawa terdiri dari dua kelompok besar yang mempunyai indeks similaritas 61,2%. Kelompok pertama adalah strain S. typhi Isolat Jawa Tengah dan Jawa Timur, dan kelompok ke dua adalah strain S. typhi Isolat Jawa Barat dan DKI. Pita 14 (12,5 kD) dan 15 (78kD) dari protein sub unit pilli hanya dimiliki strain S. typhi dari Surabaya. Pita 18(35kD) dan 20 (72kD) dari protein sub unit pilli hanya dimiliki oleh Strain S. typhi dari DKI Jakarta.Kata kunci: Protein Pilli, Hubungan similaritas, Salmonella typhi
ISOLASI BAKTERI PENGHASIL ENZIM PROTEASE BACILLUS THURINGIENSIS IRODI PADA ONCOM MERAH PASCA FERMENTASI 24 JAM Radna Safitri; Sakti Imam Muchlissin; Ana Hidayati Mukaromah; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

The need for protease enzymes in Indonesia and the world continues to increase, requiring  new  protease  sources.  Bacteria  are  beneficial  sources  of  protease because they are easy to obtain and rapidly multiply. Bacterial identification could be done molecularly through analysis of the 16S rRNA gene. This study aimed to obtain an isolate of protease-producing bacterium from 24-h post-fermented red oncom and to identify the obtained bacterial strain molecularly by 16S rRNA gene sequence. The protease production test on bacteria found in red oncom sample was done  using  a  selective  medium,  Skim  Milk  Agar  (SMA).  DNA  genomes  of proteolytic bacterial cells were extracted by Promega KIT. The amplifying process of 16S rRNA gene using the Polymerase Chain Reaction (PCR) method. The amplified  DNA  were  analyzed  using  the  BLAST  program.  The  results  of  the research found 8 isolate of bacterias. The most unique isolate was IROD1.3. It has significant  proteolytic  activity  based  on  the  ability  to  produce  clear  zone  of protease on SMA medium with a diameter of 85,00 mm. Isolate IROD1.3 was identified  molecularly  as  Bacillus  thuringiensis  with  similarity  of  96% to  the sequence of 16S rRNA gene Bacillus thuringiensis strain TERI SID4 (Genbank access code: KX822158.1). Keywords: Protease, 16S ribosomal RNA gene, red oncom, proteolytic bacterium
HAEMAGGLUTINATION ACTIVITY OF Salmonella typhi FLAGELLIN PROTEIN BASED ON ABO BLOOD GROUP Ragil Saptaningtyas; Sri Darmawati; Sri Sinto Dewi
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Student Paper Presentation The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Salmonella typhi is a negative basil Gram bacterium that causes typhoid fever. Flagel of S. typhi contain proteins, as locomotor and it can help bacteria to attach on host cells. Haemagglutinin protein is protein that can agglutinate erythrocytes. The goal of this research is to analize S. typhi flagellin proteins haemagglutination activity Based on ABO blood group. Flagellin proteins isolation method of modified Alexan method (2009) and haemagglutination test method of Finkeltein and Hanne method (1982). Haemagglutination result of ABO blood type show that SLT-1 S. typhi flagellin proteins can’t agglutinate human erythrocytes A, B, and O, but it can agglutinate human erythrocytes until 8 times dilution of AB blood type from 50 μg/μl concentration in 50 μl. BA 07.4 S. typhi flagellin proteins can agglutinate human erythrocytes of A blood type until 16 times dilution, 8 times dilution of AB blood type, 4 times dilution of O blood type, and it can’t agglutinate human erythrocytes B blood type.Keywords: Salmonella typhi, Flagellin Proteins, Haemagglutination, Erythrocytes of ABO System.
Molecular Characterization And Hemagglutination Activities of Flagellin Protein of Salmonella typhi Sri Darmawati; Budi Santosa; Muhammad Evy Prastiyanto; Ragil Saptaningtyas
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2016: Proceeding of International Seminar on Education Technology (ISET) 2016
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Abstract. The purposes of this research are for molecular characterization and hemagglutination activity test of flagellinprotein of Salmonella typhi. The research samples consist of 7 strains of S. typhi isolates from Central Java (5 strains from Semarang city, 1 strain from Salatiga and 1 strain from Magelang) and 2 strains of S. typhi from Yogyakarta (Doctor Sardjito Hospital and Bethesda Hospital). The undertaking procedures are: 1) PCR and sequencing of fliC genes using primer LPW 1856 and LPW 1857.2) Isolation and separation of flagellin protein using SDS-PAGE. 3) Hemagglutination Activity Test upon human erythrocytes of blood group A, B, AB and O.The results show that 8 strains of S. typhi have a fliC gene size of 1452 to 1488 bp including serovar H1-d, and 1 strain with the size of 1267 bp including serovar H1-J. Flagella protein resulted from SDS-PAGE protein consists of 1-2 major proteins and 1-3 minor proteins with a molecular weight of 16-116 kDa. The results of hemagglutination activity test of flagellin protein show that there are 3 strains of S. typhi (MG-1, SA02.2 and BET) which are able to agglutinate human erythrocytes of bloodgroup A, B, AB and O (2-64HA), 6 other strains show various hemagglutination activities varied
DAYA HAMBAT DAUN ASAM JAWA (Tamarindus indica) TERHADAP PERTUMBUHAN Salmonella typhi PENYEBAB DEMAM TIFOID Dini Puspodewi; Sri Darmawati; Endang Triwahyuni Maharani
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2015: Prosiding Student Paper Presentation The 2nd University Research Colloquium
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tifoid fever is endemic disease who caused by Salmonella typhi bacteria and still to be seriously health problem in Indonesia. The treatment still use antibiotic who can cause resistant if uncontrol using. Tamarind (Tamarindus indica) is plant who can use as medicine. The leaves contain flavonoid, tannin, and saponin who can function as antibacteria. The purpose of this research to know inhibition of tamarind leaves to Salmonella typhi who caused tifoid fever in 25% consentration. This research using old tamrind leaves from Rembang. The leaves was drying and powdering, then soxhletation with eter, extraction with maceration and infused method using distiled water with 25% concentration, then freeze dying and make 1, 2, 3, 4, and 5 mg/25 μL concentration per disc in a row. antibacteri tested with Kirby bauer method. The result of this experiment showing all of variance concentrations at disc can inhibit Salmonella typhi BA07.4 in maceration method as well as in infused method in 25% concentration of tamarind leaves. 5 mg/25 μL concentration has large antibacterial essence showed with existence large zone of inhibition 11,5 mm in maceration method and 14 mm in infused method. infused method of 25% tamarind leaves is more antibacterial essence than maceration method.Keyword : Tamarind leaves, Salmonella typhi, Tifoid fever
ISOLASI BAKTERI PENGHASIL ENZIM PROTEASE BACILLUS MEGATERIUM IROD3 DARI ONCOM MERAH PASCA FERMENTASI 72 JAM Dhea Ayu Lestari; Sakti Imam Muchlissin; Ana Hidayanti Mukaromah; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Protease  enzyme  has  function  to  hydrolyze  peptide  bonds  in  proteins  into simpler molecules to digest by the body which important to food industry. One of effort to increase the production of protease enzymes is looking for new sources of protease particularly from bacterial groups. The purpose of this study was to obtain an isolate of protease-producing bacteria found on post- fermentation oncom 72 hours, and to identify the protease-producing bacteria based on the analysis of 16S rRNA gene. Isolation and purification process of bacterial colony was carried out on Nutrient Agar medium with spread technique, production test of protease enzyme was performed using Selective Skim Milk Agar. The process of Molecular identification process was carried out  through  analysis  of  16S  rRNA  gene  fragment  sequences  which  were amplified using Polymerase Chain Reaction (PCR) method, and continued by sequencing. The result of bacteria isolation was found one isolate which has proteolytic activity in Skim Milk Agar medium which has clear zone diameter of 78.00 mm. A similarity analysis based on the 16S rRNA gene sequence showed that IROD3 (Indonesian Red Oncom Day-3) has 99% similarity level with the 16S rRNA gene fragment of Bacillus megaterium strain CS17 (access code Genbank: MG430224.1). Keywords:  Molecular  identification,  proteolytic  bacteria,  16S  rRNA  gene, Bacillus megaterium
Co-Authors - Anawar S Ana Hidayanti Mukaromah Ana Hidayati Mukaromah Aprilia Indra Kartika Asri Rahmiati Aulia Harun Ayu Rahmawati Sulistyaningtyas Budi Santosa Deastika Widianingratri Defi Nurul Hayati Dhea Ayu Lestari Dini Puspodewi Dwi Pamaya Endang Tri Wahyuni Maharani Endang Triwahyuni Maharani Fandhi Adi Wardoyo Fatikhin - Haris Susena Idayanti . . Iin Inayatul Karomah Insannul Kurniasari Juni Nauli Laksono Trisnantoro Langkah Sembiring Masashi Kawaichi Meutia Srikandi Fitria Misno Sudarmadi Mudyawati Kamaruddin Muh. Amin Muhammad Evy Prastiyanto Nevi Kustia Nurasia Nurasia Nurrahman Nurrahman Nursaima Siregar Radna Safitri Ragil Saptaningtyas Rahma Asriani Panjaitan Ratih Haribi Rieke Fadhila Rinda Aulia Utami Rizka Ayu Wahyuni Rizka Yolanda Febiaocti Sakti Imam Muchlissin Sayono Sayono Sindi Setiawati Ali Utami Sri Dewi Haryati Sri Sinto Dewi Sri Suhartati Stalis Norma Ethica TRI HARTITI Ulfa Nurullita Vivi Yosafianti Pohan W T Artama Wa Ode Inayatul Wa Ode Jariah M Wayan T. Artama Widya Asmara Wildiani Wilson