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All Journal PROSIDING SEMINAR NASIONAL Jurnal Kesehatan Masyarakat Indonesia JURNAL LITBANG JURNAL KESEHATAN FIKkeS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Biomedika Prosiding Seminar Nasional Unimus
Sri Darmawati
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Journal : PROSIDING SEMINAR NASIONAL

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ANALISIS IMUNOGENISITAS PROTEIN 58 kDa Salmonella typhi Sri Darmawati; Syaiful Anwar
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2014: PROSIDING SEMINAR NASIONAL HASIL - HASIL PENELITIAN & PENGABDIAN
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tujuan penelitian untuk mengetahui  imunogenisitas protein 58 kDa dari Strain Salmonellatyphi BA 07.4 dan spesifisitas antibodi yang ditimbulkannya  terhadap protein sel bakteri bentukbatang Gram negatif anggota familia Enterobacteriaceae dari kultur darah pasien Widal positif(Escherichia coli, Enterobacter cloacae, Serratia marcescens, Klebsiella pneumoniae). Metode yang digunakan adalah isolasi total protein bakteri, separasi total protein dengan metode SDS-PAGE, isolasi protein 58kDa untuk imunisasi ayam, imunisasi ayam Loghman secara subkutan dilakukan 3 kali, isolasi antibodi poliklonal dari kuning telur ayam, dan imunoblotting.Hasilnya menunjukkan bahwa protein 58kDa dari Salmonella typhi BA 07.4 bersifat immunogenik, karena dapat menimbulkan terbentuknya antibodi,   tetapi tidak spesifik  karena selain mengenali protein 58kDa yang dimiliki oleh Salmonella typhi BA07.4, juga   bereaksidengan protein yang dimiliki oleh bakteri anggota familia Enterobacteriaceae yang lain(Escherichia coli, Enterobacter cloacae, Serratia marcescens, Klebsiella pneumoniae)Kata Kunci: Salmonella typhi, protein 58 kDa.
ISOLASI DAN IDENTIFIKASI MOLEKULER BAKTERI PENGHASIL ENZIM PROTEASE PSEUDOMONAS STUTZERI ISTD4 DARI TEMPE GEMBUS PASCA FERMENTASI 1 HARI Wa Ode Inayatul; Sakti Imam Muchlissin; Ana Hidayati Mukaromah; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Proteaseis  a group  of enzymes that  play  an important  role in  biochemical reactions, whichc a use protein break down. Protease is among main enzymes used in industry, which commercial value reach 60% of total enzymes world wide. This study a imed toisolat protease-producing bacterium found on tempe gembus in after 1-day post-fermentation and to identify the bacterial isolat obtained based on the analysis of its 16S rRNA gene. Isolati on and purification process wasd one using Nutrient Agar media with spread technique. The protease production test was carried out on skim milk agar medium. The molecular identification process was performed by analyzing sequence of 16S rRNA gene fragment of bacteria amplified using both forward primer F (F:5'- AGAGTTGATCCTGGCTCAG-3'), and reverse primer R (R:5'- GGTTACCTTGTTACGACTT-3') by Polymerase Chain Reaction (PCR) method. The amplified DNA from PCR was then sequenced. From the isolation process a bacterial strain that has a proteolytic activity based on observation of clear zone area with a diameter of 85 mm was obtained. From sequence alignment result using BLAST (Basic Local Alignment Search Tool) the fragment of 16S rRNA gene of strain ISTD1.4 obtained has similarity level of 98% with fragment of 16S ribosomal RNA gene of bacterium Pseudomonas stutzeri strain E141. In conclusion, strain ISTD1.4 is a potential protease-producing bacteria and is identified as Pseudomonas stutzeri ISTD4. Keywords: Bacterial isolation, molecular identification, proteolytic bacteria, 16S rRNA gene
ISOLASI BAKTERI PENGHASIL ENZIM PROTEASE BACILLUS AMYLOLIQUEFACIENS IROD2 PADA ONCOM MERAH PASCA FERMENTASI 48 JAM Dwi Pamaya; Sakti Imam Muchlissin; Endang Tri Wahyuni Maharani; Sri Darmawati; Stalis Norma Ethica
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Proteolytic  bacteria  are  the  bacteria  capable  of  producing  extracellular protease enzymes, namely protein-breaking enzymes that are widely used in many industrial fields. This study aimed to isolate a proteolytic bacterium found on 48-h post-fermented oncom and molecular identification method. The initial isolation and purification process of the colony was carried out using Nutrient Agar medium. Selection of protease enzyme obtained by bacterial isolate was done on Milk Skim Agar medium. Identification process of the isolate was done through amplification of 16S rRNA gene using PCR, sequencing and analysis of gene sequences using BLAST program. From the isolation process  a bacterial isolate that has proteolytic by the ability to produce a clear zone of 82.00 on plate. The result of the 16S rRNA gene sequence analysis showed that the proteolytic bacterial isolate obtained in this study had a 98% homology level with 16S ribosomal RNA isolate of Bacillus amyloliquefaciens strain A1142 (Genbank access code: KTT722836.1). Based on the results of the molecular identification, the isolate was identified as Bacillus amyloliquefaciens strain IROD2  (IROD2 = Indonesia Red Oncom Day2). As conclusion, from 48-h post fermented red oncom, a protease producing bacterial strain molecularly identified as Bacillus amyloliquefaciens strain IROD2. Keywords: Moleculary was identified, Proteolitic bacteria, 16S rRNA gene
PROFIL PROTEIN DAGING KAMBING, KERBAU DAN SAPI YANG DIRENDAM LARUTAN JAHE BERBASIS SDS-PAGE Rieke Fadhila; Sri Darmawati
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Ginger contains protease enzyme and that is Zingibain which can hydrolyze proteins so it can soften meat. The aim of this study is to analyze protein profiles in goat, buffalo and cow meats before and after those meats are soaked with ginger solution in 4% v/v, 6% v/v, 8% v/v and 10% v/v concentrations for 30 minutes. Protein profile of meats was analyzed with SDS-PAGE method. The result of this study shows that control meats which are not soaked with ginger solution have many major protein bands while sample meats which have soaked with ginger solution have many minor protein bands. The result of this study shows thatsoaking meats with ginger solution can soften meats because zingibain enzyme in ginger solution can break peptide bonds in protein of meat so it forms micromolecules (minor bands). Ginger solution which is the best to soak goat, buffalo and cow meats is on 4% concentration because there are so many proteins in those meats. The higher ginger solution concentration is the more and more zingibain enzyme content are so the ability to hydrolize protein is higher marked with slackening major protein bands and increasing minor protein bands. Keywords: Meat, Ginger, Protein Profiles, SDS-PAGE
AKTIVITAS ANTIBAKTERI MADU TERHADAP BAKTERI MULTI DRUG RESISTANT Salmonella typhi DAN METHICILLIN-RESISTANT Staphylococcus aureus Rahma Asriani Panjaitan; Sri Darmawati; Muhammad Evy Prastiyanto
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2018: SEMINAR NASIONAL PENDIDIKAN SAINS DAN TEKNOLOGI
Publisher : Universitas Muhammadiyah Semarang

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Abstract

This study aimed to analyze the antibacterial activity of Palm oil Honey against Multi  Drug  Resistant  Salmonella  typhi  and  Methicillin-Resistant Staphylococcus aureus by concentration 50%, 60%, 70%, 80%, 90% and 100%. This  research  was  an  experimental  test  with  Posttest  Only  Control  Group Design in an in vitro manner using well diffusion method techniques. The well diffusion method used MHA which made by the well of 5 mm diameter and inserted 200 µ L sample then incubated 35 ± 20C for 24 hours. The results of the study showed the antibacterial activity of Palm oil Honey with zone inhibition against MDR S.typhi 11.4 mm (concentration 90%), 13.4 mm (concentration 100%) and against MRSA 11.7 mm (concentration 100%). The results of zone inhibition of Palm oil honey with concentration 100% against MDR S.typhi was larger than the  zone inhibition  against MRSA. The palm oil honey inhibition zone  against  S.typhi  MDR  bacteria  compared  with  sulfamethoxazole  (SXT) antibiotics with 25 mm inhibition zones was included in the intermediate category and the palm oil honey inhibition zone against MRSA bacteria compared with Tetracycline (TE) antibiotics with 23 mm inhibition zone was included in resistant category. Keywords: Antibacterial Activity, MDR S. typhi, MRSA, Honey, Inhibition Zone
DAYA HAMBAT EKTRAK ETANOL BUAH BELIMBING WULUH (Averrhoa bilimbi L) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Staphylococcus epidermidis SECARA IN VITRO Asri Rahmiati; Sri Darmawati; Ana Hidayati Mukaromah
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Acne infection is caused inflammation of pilosebasea accompanied by accumulation of keratin material, caused by S. aureus and S. epidermidis bacteria. The community uses of wuluhstarfruit (Averrhoabilimbi L) as a traditional medicine to treat acne infection. Wuluh starfruit contains flavonoids, alkaloids, tannins, and saponins that act as anti microbial. The aim of this study was to analyze the inhibitory power of wuluh starfruit ethanol extract on the growth of S. aureus and S. epidermidis bacteria. The method used in this research is the diffusion of wells. This research used two types of bacteria. S.aureus and S.epidrmidis, each bacteria of the four treatment groups that is 10%w/v; 20%w/v; 30%w/v; 40%w/v; positive control of Ciprofloxacin, and negative control of sterile aquades. The research results of inhibitory power of ethanol extract of wuluh starfruit with variation of concentration 10 %w/v; 20 %w/v; 30 %w/v; and 40 %w/v successively in S.aureus was 21.6 mm; 27.0 mm; 31.3 mm; And 34.0 mm, whereas in S.epidermidis is 28.6 mm; 31.6 mm; 36.3 mm; And 39.0 mm. Then the positive control of Ciprofloxacin has an inhibit zone of 30.0 mm and 35.0 mm. While the negative control of sterile aquades is not formed inhibit zone. The result ofOne Way Anova statistic test on S. aureus is p=0.000 and S. epidermidis is p=0.000, because (p<0.05) hence result there is significant difference, so it can be concluded that the extract of wuluh starfruit ethanol can inhibit the growth of S. aureus and S. epidermidis and there is a significant difference between the variantconcentration ofethanol extract wuluhstarfruit. Keywords: Staphylococcus aureus, Staphylococcus epidermidis, Ethanol extract of wuluhstarfruit.
Analisis Molekuler Profil Protein Pilli untuk Mengungkap Hubungan Similaritas 26 Strain Salmonella typhi Isolat Jawa Sri Darmawati; Ratih Haribi; Syaiful Anwar
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2012: SEMINAR NASIONAL HASIL PENELITIAN 2012
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Variasi dan hubungan similaritas 26 strain Salmonella typhi Isolat Jawa merupakanawal untuk melacak protein sub unit pilli spesifik yang memiliki aktivitas hemaglutinasi.Tujuan penelitian analisis molekuler profil protein pilli untuk mengungkap hubungansimilaritas 26 Strain S. typhi Isolat Jawa.Analisis dilakukan terhadap 26 strain yang berasal dari Surabaya, Madiun,Malang, Salatiga, Magelang, Bandung, Bogor, Jakarta, Yogyakarta dengan elektroforesisSDS-PAGE. Analisis hubungan similaritas digunakan program MVSP, untukmengkonstruksi dendogram yang mencerminkan klasifikasi dari 26 strain S. typhi IsolatJawa berdasarkan nilai indeks similaritas (S SM ) dengan algoritma UPGMA.Hasil analisis profil protein pili menunjukkan (1) Jumlah pita protein sub unitpilli bervariasi : 8-17 pita, BM tertinggi 200 kD, terendah 10 kD, dengan 20 karakter. (2)Protein 100 kD, 50 kD, 45 kD dan 40 kD adalah protein sub unit pilli yang dimiliki oleh26 strain S. typhi Isolat Jawa. (3) Dari 26 strain S. typhi Isolat Jawa terdiri dari duakelompok besar yang mempunyai indeks similaritas 61,2%. Kelompok pertama adalahstrain S. typhi Isolat Jawa Tengah dan Jawa Timur, dan kelompok ke dua adalah strain S.typhi Isolat Jawa Barat dan DKI. Pita 14 (12,5 kD) dan 15 (78kD) dari protein sub unitpilli hanya dimiliki strain S. typhi dari Surabaya. Pita 18(35kD) dan 20 (72kD) dariprotein sub unit pilli hanya dimiliki oleh Strain S. typhi dari DKI Jakarta.
DETEKSI GEN Coa PADA Staphylococcus aureus YANG DIISOLASI DARI SUSU SAPI MURNI Rizka Ayu Wahyuni; Sri Darmawati; Muhammad Evy Prastiyanto
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Publikasi Hasil-Hasil Penelitian dan Pengabdian Masyarakat
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Tujuan penelitian ini untuk mendeteksi gen Coa pada S. aureus yang diisolasi dari susu sapi murni di Kelurahan Gedawang. Jenis penelitian ini adalah penelitian deskriptif. Bakteri S. aureus di isolasi dari 15 sampel susu sapi murni. Deteksi gen Coa S. aureus menggunakan primer spesifik  Forward (5´-ATAGAGATGCTGGTACAGG-´3) dan primer Coa Reverse (5´GCTTCCGATTGTTCGATGC-3´).Jumlah isolat S. aureus yang didapatkan dari Isolasi Bakteri sebanyak 3 sampel positif yang memiliki gen Coa dengan hasil produk 756 bp. Hasil pada penelitian ini menunjukkan hasil positif gen Coa sebesar 100%.   Keywords: Staphylococcus aureus, Susu, gen Coa
PROFIL PROTEIN TIGA JENIS DAGING YANG DILUMURI SERBUK DAUN PEPAYA BERBASIS SDS-PAGE Nevi Kustia; Sri Darmawati; Fandhi Adi Wardoyo
PROSIDING SEMINAR NASIONAL & INTERNASIONAL 2017: Prosiding Seminar Nasional Pendidikan, Sains dan Teknologi
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Papaya leaves contain protease enzymes (protein decomposers), namely papain and kimopapain. Both of these enzymes have the ability to break bonds in protein molecules so that proteins break down into polypeptides and dipeptides. If working on the meat can be decomposed so that the meat becomes tender. The purpose of this study was to determine protein profiles in three types of meat before and after smeared with papaya leaf powder with 60 minutes of immersion. The protein profile of three meat types was analyzed using the SDS-PAGE method. The design of this research is descriptive research with the object of research are goat meat, buffalo and cow covered with papaya leaf powder. The result of this research shows that there are meat of buffalo, goat and cow there are many major and minor ribbon, while in buffalo meat, goat and cow which has been smeared with papaya powder there are many minor protein bands. These results indicate that papain enzyme contained in papaya leaves are powdered to break peptide bonds in meat proteins to proteins in the form of minor bands (micromolecules) and show that the higher concentration of papaya leaf powder the more denatured the protein that is on 20% concentration has only 3 protein bands.Keywords: Meat, Papaya Leaf, Protein Profile, SDS-PAGE
Co-Authors - Anawar S Ana Hidayanti Mukaromah Ana Hidayati Mukaromah Aprilia Indra Kartika Asri Rahmiati Aulia Harun Ayu Rahmawati Sulistyaningtyas Budi Santosa Deastika Widianingratri Defi Nurul Hayati Dhea Ayu Lestari Dini Puspodewi Dwi Pamaya Endang Tri Wahyuni Maharani Endang Triwahyuni Maharani Fandhi Adi Wardoyo Fatikhin - Haris Susena Idayanti . . Iin Inayatul Karomah Insannul Kurniasari Juni Nauli Laksono Trisnantoro Langkah Sembiring Masashi Kawaichi Meutia Srikandi Fitria Misno Sudarmadi Mudyawati Kamaruddin Muh. Amin Muhammad Evy Prastiyanto Nevi Kustia Nurasia Nurasia Nurrahman Nurrahman Nursaima Siregar Radna Safitri Ragil Saptaningtyas Rahma Asriani Panjaitan Ratih Haribi Rieke Fadhila Rinda Aulia Utami Rizka Ayu Wahyuni Rizka Yolanda Febiaocti Sakti Imam Muchlissin Sayono Sayono Sindi Setiawati Ali Utami Sri Dewi Haryati Sri Sinto Dewi Sri Suhartati Stalis Norma Ethica TRI HARTITI Ulfa Nurullita Vivi Yosafianti Pohan W T Artama Wa Ode Inayatul Wa Ode Jariah M Wayan T. Artama Widya Asmara Wildiani Wilson