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All Journal PROSIDING SEMINAR NASIONAL Jurnal Kesehatan Masyarakat Indonesia JURNAL LITBANG JURNAL KESEHATAN FIKkeS Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Biotechnology Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Biomedika Prosiding Seminar Nasional Unimus
Sri Darmawati
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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Journal : JURNAL LITBANG

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ANALISIS PROTEIN PILLI Salmonella typhi lsolat RS. Kariadi Semarang DENGAN ELEKTROFORESIS SDS-PAGE Sri Darmawati; Ratih Haribi
JURNAL LITBANG Vol 2, No 3 (2005): Penelitian, Pengembangan, dan Pengabdian
Publisher : JURNAL LITBANG

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Abstract

Background : Salmonella typhi is bacteria which causes typhoid fever. Typhoid fever is severally serious, infectious decease and is endemic decease in Indonesia with relatively high frequency rate around 358-810/100.000 people each year This decease is usually spreading along with many kinds of decease which also have relatively high mortality rate, that is 1-5% of the sufferers (Punjabi, NH. , 2 004) . The infection process of bacteria into human body is signified with bacteria Cell adhering in mucosa intestinal surface. Pilli is structured by pilli protein consisting of Several sub units of pilli protein Objective: conducting pilli protein Salmonella typhi isolate Kariadi Hospital Semarang Analysis through electrophoreses SDS-PAGE, so that take amount of sub unit of pilli protein with each its molecule weight can be found out. Method : This research is conducted through three stages; firstly, bacteria cultivation with Biphasic media (BHI Agar and broth BHI ); secondly, pilli isolation with Ehara method (/956); Thirdly, sub unit of pilli protein separation with I 2% SDS-PAGE based on Lemmli method (1970). Result: There are four major sub units of protein in pilli, that is, 36kDa; 26,5 kDa; 22,2kDa; 18,6lrDq and there are also four minor sub units of protein pilli, that is, 116 kDa; 62,3kDa;45kDa;20,9kDa,andseveral other minor sub units of protein with every thin band. Keywords : Salmonella and Electrophoresis
Co-Authors - Anawar S Ana Hidayanti Mukaromah Ana Hidayati Mukaromah Aprilia Indra Kartika Asri Rahmiati Aulia Harun Ayu Rahmawati Sulistyaningtyas Budi Santosa Deastika Widianingratri Defi Nurul Hayati Dhea Ayu Lestari Dini Puspodewi Dwi Pamaya Endang Tri Wahyuni Maharani Endang Triwahyuni Maharani Fandhi Adi Wardoyo Fatikhin - Haris Susena Idayanti . . Iin Inayatul Karomah Insannul Kurniasari Juni Nauli Laksono Trisnantoro Langkah Sembiring Masashi Kawaichi Meutia Srikandi Fitria Misno Sudarmadi Mudyawati Kamaruddin Muh. Amin Muhammad Evy Prastiyanto Nevi Kustia Nurasia Nurasia Nurrahman Nurrahman Nursaima Siregar Radna Safitri Ragil Saptaningtyas Rahma Asriani Panjaitan Ratih Haribi Rieke Fadhila Rinda Aulia Utami Rizka Ayu Wahyuni Rizka Yolanda Febiaocti Sakti Imam Muchlissin Sayono Sayono Sindi Setiawati Ali Utami Sri Dewi Haryati Sri Sinto Dewi Sri Suhartati Stalis Norma Ethica TRI HARTITI Ulfa Nurullita Vivi Yosafianti Pohan W T Artama Wa Ode Inayatul Wa Ode Jariah M Wayan T. Artama Widya Asmara Wildiani Wilson