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All Journal BALI MEDICAL JOURNAL (BMJ) Jurnal Veteriner Media Penelitian dan Pengembangan Kesehatan Berkala Ilmu Kesehatan Kulit dan Kelamin Jurnal NERS Majalah Obat Tradisional Journal of Islamic Pharmacy Cakrawala: Jurnal Litbang Kebijakan Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia SURABAYA PHYSICAL MEDICINE AND REHABILITATION JOURNAL Indian Journal of Forensic Medicine & Toxicology Indonesia Journal of Physical Medicine and Rehabilitation Indonesia Journal Physical Medicine and Rehabilitation Farmasis: Jurnal Sains Farmasi Borneo Journal of Pharmacy Journal of Tropical Pharmacy and Chemistry Jurnal Sains dan Kesehatan Folia Medica Indonesiana
Hening Laswati Putra
Departemen Kedokteran Fisik Dan Rehabilitasi, Fakultas Kedokteran, Universitas Airlangga, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary

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Journal : Jurnal NERS

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HUBUNGAN EKSPRESI HSP 70 DAN EKSPRESI RESEPTOR OPIOID MU PADA PENURUNAN NYERI TERAPI BEKAM BASAH Subadi, Imam; Laswati, Hening; JM, Harjanto
Jurnal NERS Vol 11, No 1 (2016): Jurnal Ners
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.452 KB) | DOI: 10.20473/%oj.Ners111%y34-39

Abstract

Latar belakang. Terapi bekam basah banyak digunakan untuk mengurangi nyeri,  namun mekanisme penurunan nyeri terapi bekam basah belum jelas. Peregangan dan tusukan pada terapi bekam basah menimbulkan stres pada sel. Sel yang mengalami stres mengekspresikan HSP 70 dan reseptor opioid mu.Tujuan penelitian. Penelitian ini bertujuan membuktikan korelasi  peningkatan ekspresi HSP 70 dan peningkatan reseptor opioid mu pasca terapi bekam basah.Bahan dan Metode. Jenis penelitian ini adalah penelitian eksperimental dengan rancangan randomized control group post test only design. Dua puluh empat tikus jenis Wistar (Rattus norvegicus)  secara random dibagi menjadi tiga kelompok yang masing-masing terdiri dari 8 ekor tikus yaitu kelompok kontrol negatif (tikus normal), kelompok induksi nyeri dengan Complete Freund’s Adjuvant (CFA) saja (kontrol positif) dan kelompok  yang diberi induksi nyeri dan terapi bekam basah (10 tusukan dan tekanan negatif: - 200 mmHg, 5 menit).  Sampel diambil dari kulit dan dilakukan pemeriksaan imunohistokimia dengan antibodi monoklonal anti reseptor opioid mu. Waktu reaksi ambang nyeri diukur memakai hot-plate. Data dianalisa dengan Anova oneway dan Pearson Correlation dengan menggunakan SPSS versi 17.Hasil. Penelitian ini menunjukkan bahwa terdapat peningkatan ekspresi HSP 70 (20,25 ± 3,53; p< 0.05) dibandingkan kelompok kontrol positif (10,50 ± 2,44; p< 0,05) dan peningkatan ekspresi reseptor opioid mu  (21,00 ± 6,34; p< 0.05) dibandingkan kelompok kontrol positif ( 4,25 ± 3,58; p< 0.05). Terdapat peningkatan waktu reaksi ambang nyeri (22,81 ± 6,34; p< 0,05) dibandingkan kontrol positif (11,78 ± 3,56). Terdapat korelasi antara peningkatan ekspresi HSP 70 dan peningkatan reseptor opioid mu  (β= 0.893; p= 0.000) dan ada korelasi antara peningkatan reseptor opioid mu dengan waktu reaksi ambang nyeri (β= 0.713; p= 0.002).Kesimpulan. Terjadi peningkatan ekspresi HSP 70 dan reseptor opioid mu pada terapi bekam basah dan terdapat korelasi antara peningkatan ekspresi HSP 70 terhadap ekspresi reseptor opioid mu.Kata kunci : HSP 70, reseptor opioid mu dan bekam basah 
Ekspresi HSP 70 dan Ekspresi Reseptor Opioid Mu pada Penurunan Nyeri Terapi Bekam Basah Imam Subadi; Hening Laswati; Harjanto JM
Jurnal Ners Vol. 11 No. 1 (2016): April 2016
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.452 KB) | DOI: 10.20473/jn.v11i1.1901

Abstract

Introduction: Wet cupping therapy widely used to reduce the pain, but the mechanisms of pain reduction on wet cuppig therapy is unclear. Stretching and punctures on wet cupping therapy cause stress on the cell. Cells that are stressed express HSP 70 and mu opioid receptors. This study aims to prove the correlation increased expression of HSP 70 and an increase in post-mu opioid receptor wet cupping therapy.Methods: The study is an experimental research design with randomized control group post-test only design. Twenty-four rats type Wistar (Rattus norvegicus) were randomly divided into three groups, each consisting of eight mice are negative control group (normal mice), group induction of pain with Complete Freund's Adjuvant (CFA) alone (positive control) and group by induction of pain and bekam basah therapy (10 puncture and negative pressure: - 200 mmHg, 5 minutes). Samples were taken from the skin and immunohistochemical examination with the monoclonal antibody anti mu opioid receptors. The reaction time pain threshold measured using a hot-plate. Data were analyzed by oneway ANOVA and Pearson Correlation using SPSS version 17.Results: This study shows that there is an increased expression of HSP 70 (P <0.05) compared to the positive control group (p <0.05) and increased expression of mu opioid receptors ( p <0.05) compared to the positive control group (p <0.05). There is an increased pain threshold reaction time (p <0.05) compared to the positive controls (11.78 ± 3.56). There is a correlation between an increased expression of HSP 70 and an increase in mu opioid receptor (β = 0893; p = 0.000) and there was a correlation between the increase in mu opioid receptor with a reaction time of pain threshold (β = 0713; p = 0.002).Discussion: Wet cupping therapy increase HSP 70 expression, opioid mu receptor, and decrease pain level.
Co-Authors Agnis Pondineka Ria Aditama Agustina Konginan Amalia, Dilla Ananda, Naoval Diza Andriana, RA Meisy Anisah Mahardiani Anita Ratnawati Arief Suryadinata Artabah Burhan Ma&#039;arif Burhan Ma'arif Burhan Ma'arif Burhan Ma’arif Burhan Ma’arif Burhan Ma’arif Damayanti - Denis Dian Irawati Dian Marta Sari Dilla Amalia Eden Leonita Faisal Akhmal Muslikh Fazia Firdausy, Alif Firman Guhir, Abdul Malik Harjanto JM Harjanto JM Hasni Hasni Hendy Hendarto Ida Yuanita Ikhwan Muhammad Imam Subadi J. A. Pangkahila Jatmiko, Ricky Arie L. Maha putra L. Maha Putra Laba Mahaputra Luthfi Achmad Muchlasi M. Artabah Muchlisin Ma'arif, Burhan Mangestuti Agil Marta Ika Riyana Martha Kurnia Kusumawardani Maximus M Taek Mei Wulan, Sri Mardjiati Meilina Ratna Dianti Muslim, Menara Nabila Rosa Nabilah Ishanan Ramadhan Nasronudin Nasronudin Nuniek Nugraheni Nurwijayanti Nury Nusdwinuringtyas Patricia Maria Kurniawati Paulus, Anitta Florence Stans Poerwandari, Dewi Pramudita Riwanti Prawitri, Yudith Dian Primitasari, Yulia Purbosari, Ira Rahmi Annisa Rejeki, Purwo Sri Retno Widyowati Ria Ramadhani Dwi Atmaja Riyanti, Anisa Eka Rozalina Loebis, Rozalina Saidah, Nisfatul Lailatus Sari, Dewi Perwito Setiawati, Rosy Suyoko, Andri Tedjasukmana, Deddy Trisniartami Setyaningrum Usman Hadi Wardhani, Indrayuni Lukitra Wicaksono Alim Budihartanto Widjanantie, Siti Chandra Wigati, Kristanti Wanito Wirda Anggraini Yuanita, Ida