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EFFECT OF MODERATE INTENSITY EXERCISE ON BODY WEIGHT AND BLOOD ESTROGEN LEVEL OVARIECTOMIZED MICE Prasetya, Rizka Eka; Umijati, Sri; Rejeki, Purwo Sri
Majalah Kedokteran Bandung Vol 50, No 3 (2018)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v50n3.1368

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Estrogen is a hormone which is related to lipid oxidation, lypogenesis inhibition, and lipolysis rate. The purpose of this study was to determine the effect of moderate intensity exercise on body weight and blood estrogen level in ovarectomized mice (ovx). The study was held in the Laboratory of Biochemistry, Faculty of Medicine Universitas Airlangga during the period of April to May 2017 on old nineteen 3-4 months old female mice, , 20-30 grams of weight, which were divided into two groups: OVX (n=9, ovx without exercise) and OVX+E (n=10, ovx and moderate intensity exercise). Moderate intensity exercise in this study included swimming, three times a week at 3 pm for four weeks using a 6% of body weight load. Experiment data were analyzed by paired t-test and independent t-test using SPSS. The average body weights of the OVX  group were 26.44 ± 1.94 grams and 29.89 ± 2.62 gram at pretest and posttest, respectively., while for the OVX+E group, the weights were 26.50 ± 2.59 grams and 26.40 ± 2.07 grams. There were asignificant intergroup body weight differences (?OVX= 3.44 ± 2.55 gram and ?OVX+E= -1.00 ± 1.37 gram) with p=0.004. Blood estrogen level in OVX+E (12.04 ±10.13 ng/ml) was higher than in control (OVX= 11.24 ± 8.33 ng/ml); however, the difference was not significant (p=0.855). Moderate intensity exercise reduces body weight, without any significant increase in blood estrogen level. Hence, the body weight loss is not caused by estrogen, which may be due to the short period of exercise in this study that the estrogen has not had the time to increase.Pengaruh Olahraga Intensitas Sedang terhadap Berat Badan dan Kadar Estrogen Darah pada Mencit Ovariektomi Estrogen merupakan hormon yang berperan pada oksidasi lipid, kecepatan lipolisis, dan hambatan lipogenesis. Penelitian bertujuan mengetahui pengaruh olahraga intensitas sedang terhadap berat badan dan kadar estrogen darah pada mencit ovariektomi (ovx). Penelitian dilaksanakan di Laboratorium Biokimia Fakultas Kedokteran Universitas Airlangga, April?Mei 2017. Sejumlah 19 mencit betina berusia 3?4 bulan, berat 20?30 gram, dibagi menjadi dua kelompok: OVX (n=9, ovx tanpa perlakuan) dan OVX+E (n=10, ovx dengan olahraga intensitas sedang). Olahraga intensitas sedang dilakukan dengan renang, tiga kali seminggu pukul 15.00 WIB selama empat minggu dengan beban 6% dari berat badan. Data dianalisis dengan paired t-test dan independent t-test SPSS. Berat badan OVX pra-tes 26,44±1,94 gram dan pascates 29,89±2,62 gram. Berat badan OVX+E pra-tes 26,50±2,59 gram dan pascates 26,40±2,07. Terdapat perbedaan bermakna berat badan antarkelompok (?OVX= 3,44±2,55 gram and ?OVX+E= -1,00±1,37 gram), p=0,004. Kadar estrogen darah OVX+E (12,04±10,13 ng/mL) lebih tinggi daripada konrol (OVX= 11,24±8,33 ng/mL), tetapi tidak berbeda bermakna (p=0,855). Olahraga intensitas sedang menurunkan berat badan, tetapi tidak bermakna meningkatkan kadar estrogen darah. Penurunan berat badan tidak disebabkan oleh estrogen. Peningkatan estrogen belum terjadi mungin disebabkan oleh waktu yang kurang lama.  
Effect of Dietary Energy Density on Increasing Blood Glucose Pattern and Hunger-Satiety Sensation Ni Luh Putu Ayu Putri Sariningrat1, Purwo Sri Rejeki1, Irfiansyah Irwadi1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 2 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i2.3384

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Background: There was no data how these food density could effect on glucose and visual analog levels based on hunger-filled scales in Indonesian population. Objective To determine the effect of food energy density on glucose levels and hunger-satiety sensation. Material and Methods: Seventeen women 18-22 years of age with BMI> 25, were given low energy density breakfast (n = 9) and high energy density (n = 7). The subjects were fasted for 10 hours, fasting blood glucose levels was measured as well as hunger-satiety sensation with a visual analog scale (VAS), prior to treatment. Breakfast was started at 08.00 a.m chew up to 32 times for 15 minutes. Blood glucose levels and VAS measurement were taken back in 2 hours and 4 hours afterward. Result: There was a significant differences in glucose levels between 4 hours postpandrial and 2 hours postpandrial (p = 0.031) and also between 4 hours postpandrial and fasting state (p = 0.042) in both groups. Analysis of hunger level VAS (p = 0.02) and satiety level VAS (0.04) at 4 hours postpandrial also differ significantly. Correlation analysis between hunger level VAS and blood glucose was different significantly (p = 0.01) with r = -0.59. Conclusions: The low energy density foods produce a pattern of increasing blood glucose levels were more stable, thus, more able to suppress the sensation of hunger than high energy density.
Efek Suplementasi Omega 3 dan Latihan Fisik Terhadap Respon Inflamasi Ayubi, Novadri; Purwanto, Bambang; Rejeki, Purwo Sri
Journal of Sport Science and Education Vol 5, No 2 (2020)
Publisher : Fakultas Ilmu Olahraga, Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jossae.v5n2.p116-123

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The aims of this article is to find out how the effects of omega 3 supplementation and physical exercise on the inflammatory response. The method applied is literature review. The results showed that omega 3 supplementation and regular exercise can reduce the inflammatory response that is not controlled. Inflammation is a protective response caused by injury or tissue damage. After physical activity the body will experienced an increase in inflammatory markers, namely C-Reactive Protein (CRP), Interleukin 1 (IL-1), Interleukin 6 (IL-6), Interleukin 10 (IL-10) and Tumor Necrosis Factor-a (TNF-a). Physical exercise, especially high-intensity eccentric exercises, will cause damage to muscle fibers and delayed muscle pain (DOMS) which results in disruption to muscle strength. Muscle pain is caused by an inflammatory process due to increased levels (TNF-a) in response to muscle damage. Inflammatory response that is not controlled can be controlled by giving omega 3 supplementation. It can be concluded that omega 3 supplementation and regular exercise are proven to reduce the inflammatory response that is not controlled, soto reduce the intensity of pain which support the performance in carrying out the exercise program. 
Swimming Improves Memory Function and Decreases N-Methyl-D-Aspartate in Ageing Rats Hanik Badriyah Hidayati; Purwo Sri Rejeki; Lilik Herawati; Susi Wahyuning Asih; Suhartati Suhartati; Siti Khaerunnisa
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16883

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A single Memory impairment substantially reduces the quality of life in the elderly. It is associated with thealteration of neurotrophic (NT) factors, such as brain-derived neurotrophic factor (BDNF) and glutamatereceptor N-methyl-D-aspartate (NMDA). Exercise is often used to reduce cognitive impairment. Previousstudies show that the benefits of aerobic exercises on such impairments are correlated with increasing BDNFand preventing the production of NMDA. However, some results remain controversial. Thus, the associationbetween exercise and Memory was addressed by examining increases in BDNF and the reduction of NMDAin ageing rats. The study used a randomized, post-test-only controlled group of 30 male one-year-old ageingRattus norvegicus divided into three groups, namely, K0 (control) and K1 and K2 (aerobic swimmingexercise). K1 and K2 animals differed in the frequency of exercise, which is three and four sessions perweek, respectively. Memory was assessed using Y-maze performance. BDNF and NMDA were analyzedusing enzyme-linked immunosorbent assays. A significant improvement in memory function and reductionin the NMDA level were observed in K1 and K2 group rats (p = 0.001; p = 0.041). No significant impact onthe BDNF levels was observed (p = 0.387). Swimming may boost Memory by reducing the NMDA levelbut not by increasing BDNF. Swimming is a promising method for preventing or delaying memory loss indegenerative brain diseases. Further investigation is needed to fully understand underlying mechanisms.
Ketogenic Diet Slows Down Weight Gain in Juvenile Mus musculus with Benzopyrene as Cancer Inducer Dita Mega Utami; Lilik Herawati; Reny I’tishom; Mohammad Anam Al-Ari; Muhammad Miftahussurur; Purwo Sri Rejeki
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14709

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Objective: This study aims to analyze the ketogenic diet that slows down weight gain in juvenile Musmusculus induced with benzopyrene (BZP). Method: Thirty-six female mice (Mus musculus), 6-8 weeks old,weighted 15-25 grams were divided into six groups randomly. K1 (negative control, standard diet) inducedwith oleum olivarum, while K2 (positive control, standard diet), P1 (ketogenic diet 1), P2 (ketogenic diet 2),P3 (ketogenic diet 3) and P4 (ketogenic diet 4) induced with BZP subcutaneously in the right breast at a doseof 0.3mg/20gBB/day for 14 days. The ketogenic diet was given for the next 28 days. Data were analyzedusing the ANOVA and post hoc LSD test. Results: After 42 days, the ketogenic diet mice experiencedslower weight gain (p=0.000) because BZP induced neurotoxicity and the administration of the ketogenicdiet in juvenile mice induced neurohormonal changes. The ketogenic diet increased ketone plasma level(p=0.000) and reduced blood glucose levels (p=0.002) due to nutritional ketosis state. Conclusions: Theketogenic diet with fat:protein ratio 60:30 slows down weight gain, increased ketone levels and decreasedblood glucose levels in juvenile mice with BZP as cancer inducer.
Comparison of High-Intensity Interval Training and Moderate-Intensity Continuous Training on VO2max and Response Reaction Time in Basketball Referees Breklen Risal Ary Wibowo; Deandra Maharani Widiatmaja; Sakina; Abdurachman; Purwo Sri Rejeki
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15715

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Effect of Moderate-Intensity Acute Physical Activity on Decreasing Cortisol Levels in Obese Female Cornelius Coli; RizkaEka Prasetya; GadisMeinar Sari; Purwo Sri Rejeki
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15719

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Moderate Intensity Exercise is Most Influential in The Expression of UCP-1 in Mice Male ------ (RETRACTED) Chabib Fachry Albab; Purwo Sri Rejeki; Muhammad Miftahussurur; Soebagijo Adi Soelistijo
Health Notions Vol 3, No 12 (2019): December
Publisher : Humanistic Network for Science and Technology (HNST)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hn31207

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This article has been retracted at the request of the authors
Altered Ghrelin Secretion Pattern and Sense of Hunger by Providing Low and High Density Breakfast in Obese Female Adolescents Ni Luh Putu Ayu Putrisari Putri; Elyana Asnar; Purwo Sri Rejeki
Biomolecular and Health Science Journal Vol. 1 No. 1 (2018): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (979.135 KB) | DOI: 10.20473/bhsj.v1i1.8223

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Introduction: Ghrelin has been recognized to have a major influence on energy balance. Hence, the discovery of various regulatory factors that control ghrelin secretion may have major implications for the development of drugs and diet control. This study aims to analyze differences in ghrelin secretion pattern and prevention of hunger in the provision of breakfast in low-calorie breakfast, low energy density and high energy density in obese female adolescents.Methods: Subjects were female students aged 18-22 years with a BMI=25 kg/m², receiving low-calorie breakfast with low (n=8) and high energy density (n=8). Before the breakfast, subjects fasted for 12 hours and were subjected to plasma ghrelin level and hunger measurement using visual analog scale (VAS). Breakfast was done at 08.00, divided into three parts and each was taken for 5 minutes. Measurement of plasma ghrelin was carried out again post prandial (PP) at 2 hours and 4 hours. VAS filling was done 1 hour PP, 2 hours PP, 3 hours PP, and 4 hours PP.Result: There were differences in plasma ghrelin level, but not significant. VAS 1 analysis of hunger (p=0.040) at 4 hours PP and VAS 3 of satiety (p=0.025) was significantly different at 3 hours PP. Conclusion: Food density did not affect the plasma ghrelin levels. Low density foods are more effective to prevent hunger in 4 hours PP and increases satiety in 3 hours PP.
Effect of Physical Training Towards Body Balance in Overweight Condition Purwo Sri Rejeki; Anita Faradilla Rahim; Rizki Eka Prasetya
Biomolecular and Health Science Journal Vol. 1 No. 2 (2018): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (153.255 KB) | DOI: 10.20473/bhsj.v1i2.9966

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An overweight individual has morphological changes in the muscle resulting functional impairment and can cause reduction in body balance system. Balance is an ability to maintain both static and dynamic equilibrium on various positions. An overweight person will experience a decrease in abdominal muscle strength and center of gravity shift. Physical training has positive effects to increase body balance. Stretching exercise maintains and increases the elasticity of muscle, tendon, fascia, ligament and joint motion and improve balance. Resistance exercise increases muscle performance which has benefit effect on balance system dynamically. The proprioceptive, an important part of balance maintenance to feel joint position or body when moving, can be improved by training that performed using balance board. Mechanoreceptor that is stimulated and the adaptation of the body's balance control that is influenced by balance board training results a good proprioceptive.
Co-Authors , Sakina Abdulloh, Ido Nur Abdurachman Adi Pranoto, Adi Al arif, Mohammad Anam Alanna Sari Pratikto Alanna Sari Pratikto Amaliyah, Miftahul Nur Ananda, Naoval Diza Andrianto Andrianto Anita Faradilla Rahim Arifa Mustika Atika Atika Ayubi, Novadri Bambang Purwanto Bambang Purwanto Breklen Risal Ary Wibowo Cantika Putri Melyana Chabib Fachry Albab Chandra, Cecilia Felicia Cornelius Coli Cornelius Coli Deandra Maharani Widiatmaja Dedi Kuswandi Desi Rahmah Sari Desiana Merawati Dewangga Satria Sakti Kinasih Dewi Ratna Sari Dita Mega Utami Eka Arum Cahyaning Putri Eko Purwanto Elyana Asnar Fathil, Nur Ezza Fazleen Mohd Fathimatuzzahroh, Fathimatuzzahroh Fikri Sasongko Widyatama Filza Yulina Ade Fitry Nachamory Oemar Hakim, Faiq Amirul Hamidah Kurniasari Hamidah Kurniasari Hanik Badriyah Hidayati,* Mohammad Hasan Machfoed,* Kuntoro,** Soetojo,*** Budi Santoso,**** Suroto,***** Budi Utomo****** Hanna Goeanawan HARJANTO HARJANTO Hartono Kahar, Hartono Hasan, Helmia Hening Laswati Putra HERAWATI, LILIK Hermanto Tri Joewono Hermina Novida, Hermina Heryana, Achmad Yuniari I'tishom, Reny Ido Nur Abdulloh Ido Nur Abdulloh Imam Subadi Imam Susilo Iqbal Laksana Irmantara Subagio Irmantara Subagio Irwadi, Irfiansyah Ismi Dian Meiliana Izzatunnisa, Nabilah Karimah, Azimatul Khairina Khalfani, Muhammad Adrian Kurniasari, Hamidah Laksana, Iqbal Leonardo Lubis MEGANTARA, SANDRA Melyana, Cantika Putri Miftahul Nur Amaliyah Misbah Abdul Haris Nasution Alhanif Misbakhul Munir Mochammad Abdul Aziz, Mochammad Abdul Mohammad Anam Al-Ari Mubasyiroh, Nabila Ainaya Mufarrihul Ihsan Muhammad Adrian Khalfani Muhammad Al-Farouq Yufiro Akbar Muhammad Miftahussurur Ni Luh Putu Ayu Putrisari Putri Nova Sylviana Nur Ezza Fazleen Mohd Fathil Oemar, Fitry Nachamory Pambudi, A. Agam Haris Paulus Liben, Paulus Permana, Noah Jefferson Prasetya, Rizka Eka Qurnianingsih, Ema Raden Argarini Ray, Hamidie Ronald Daniel RizkaEka Prasetya Rizki Eka Prasetya Ronny Lesmana Rosdianto, Aziiz Mardanarian Rurus Suryawan S. Sugiharto Sakina Sakina Sakina Samsriyaningsih Handayani Saputro, Zanuar Bagus Saputro, Zanuar Bagus Sari, Gadis Meinar Savitri, Camilia Metadea Aji Septyaningrum Putri Purwoto Setiawan Setiawan Setiawan, Hayuris Kinandita Siti Khaerunnisa Soelistijo, Soebagijo Adi Soffil Yudha Mulyadi Sony Wibisono Sri Sunarti Sri Sunarti Sri Umijati Sugiharto Sugiharto Sugiharto Sugiharto Suhartati Suhartati Sukarno, Devitya Angielevi Sundari Indah Wiyasihati Suryawan, I Gde Rurus Susi Wahyuning Asih Susianti Susianti Suyoko, Andri Syahraya, Indira Texaga, Derryl Rasad Tinduh, Darmayanti Ummah, Fithriyah Cholifatul Unang Supratman Vania Islamey Kusuma Vita Murniati Tarawan Wardani, Tjitra Wardani, Tjitra Wardhani, Indrayuni Lukitra Yoshio Yamaoka, Yoshio Yudith Annisa Ayu Rezkitha, Yudith Annisa Ayu Yuliawati, Tri Hartini Yuni Susanti Pratiwi