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PENGOLAHAN SATE GURITA (Octopus sp.) di UMKM. MINA JAYA MANDIRI, PANTAI KONDANG MERAK, DESA SUMBERBENING, KECAMATAN BANTUR, KABUPATEN MALANG Yunita Eka Puspitasari; Umi Rohmatuzzahro; Hardoko Hardoko; Eddy Suprayitno; Heder Djamaluddin; Jeny Ernawati Tambunan; Mickhael A.P. Panjaitan; Anies Chamidah
Jurnal Abdi Insani Vol 11 No 3 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i3.1784

Abstract

Satay or satay is a food that is very popular with everyone and is quite popular in Indonesia, Malaysia and Singapore. Satay can be made from beef, chicken, lamb, and seafood such as tuna and shellfish. So far, octopus in the South Malang coastal area is also found in abundance during the season and is only sold in the form of fresh octopus. UMKM Mina Jaya Mandiri intends to serve processed octopus in the form of satay, so it is collaborating with the Malang Regency Fisheries and Maritime Service and Brawijaya University universities to get training on the diversification of processed octopus, in this case octopus satay. This community service program aims to (1) provide a general overview of the processing of octopus satay (2) train fish processors at Kondang Merak Beach MSMEs to make octopus satay products using a semi-mechanical grill. This community service activity was carried out by simulating the processing of octopus satay using a semi-mechanical grill. The results of community service activities at UMKM Mina Jaya Mandiri, Kondang Merak Beach show that MSME business actors are enthusiastic about making octopus satay in their respective places of business. The application of a semi-mechanical grill in making octopus satay provides a taste that is more popular with the public and is more efficient in terms of grilling time. This can encourage MSMEs to process fresh octopus into octopus satay using mechanical grilling technology and increase business actors' income from the increasing number of tourists visiting and enjoying various seafood culinary preparations at Kondang Merak Beach.
KARAKTERISTIK FISIKOKIMIA SNACK BAR TEPUNG BUAH MANGROVE API-API (Avicennia marina) Muhammad Fairuz Zenadine; Titik Dwi Sulistiyati; Hardoko Hardoko; Yunita Eka Puspitasari
Jurnal Perikanan Unram Vol 14 No 2 (2024): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v14i2.793

Abstract

Konsumsi snack bar dengan kadar gula tinggi dan rendah serat dapat menyebabkan penyakit degeneratif. Buah mangrove api-api Avicennia marina dikonsumsi sebagai makanan tradisional oleh masyarakat mangrove, sebab mengandung beberapa senyawa bioaktif yang bermanfaat untuk mengatasi penyakit degeneratif. Untuk itu perlu diformulasikan snack bar dengan penambahan tepung buah mangrove api-api (Avicennia marina) untuk meningkatkan nutrisi dan menjadi produk pangan fungsional. Tujuan dari penelitian ini adalah untuk mengetahui karakteristik fisikokimia dari snack bar yang dibuat dari tepung buah mangrove api-api. Penelitian ini dilakukan dalam dua tahap yaitu proporsi bahan kering dan bahan basah dari snack bar kemudian dilanjutkan dengan formulasi snack bar dengan penambahan tepung buah mangrove api-api Avicennia marina. Proporsi bahan kering dan bahan basah yang diteliti di tahap awal adalah sebesar 55:45 dan 65:35. Proporsi terbaik ditunjukkan pada bahan kering:bahan basah 55:45. Penambahan tepung buah mangrove api-api Avicennia marina adalah 0%, 5%, 10% dan 15%. Hasil analisis menunjukkan bahwa konsentrasi penambahan tepung buah mangrove api-api Avicennia marina sebesar 5% berpengaruh nyata pada produk snack bar dengan uji fisika yaitu tekstur 191,34; lightness 45,066; redness 9,27; dan yellowness 43,99; kadar karbohidrat sebesar 54,052. Nilai analisa proksimat yaitu kadar air 24,05%, kadar abu 1,50%, kadar protein 9,37%, kadar lemak 9,05%, dan kadar karbohidrat 54,20%. Uji organoleptik skoring warna 3,564, aroma 3,564, rasa 3,592, dan tekstur 3,456.
IN SILICO STUDY OF TRITERPENOID IDENTIFIED FROM Ceriops decandra LEAVES AS INHIBITORS OF α-AMYLASE Puspitasari, Yunita Eka; Tambunan, Jeny Ernawati; Hardoko, Hardoko
Asian Journal of Aquatic Sciences Vol. 5 No. 3 (2022): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

α-amylase has a pivotal role in catalyzing the cleavage of α-1,4-glycosidic bonds of polysaccharides to produce oligosaccharides. The inhibition of α-amylase delays the breakdown of carbohydrates, causing a reduction of blood glucose levels absorption in diabetes patients. The exploration of α-amylase inhibitors has attracted because society assumed that utilizing herbal medicine reduced the side effect of prescribed drugs. Mangrove from genus Ceriops have been used as antidiabetic, but the mechanism as α-amylase inhibitors has not been reported. Consumption of leaves extract of C.decandra reduced blood glucose level in diabetic rats, and triterpenoids have been identified from the leaves. With this in mind, this study aims to predict the molecular interactions between α-amylase (PDB ID: 4GQR) and the inhibitors, triterpenoid identified in C.decandra leaves, and to evaluate the potency of triterpenoid as α-amylase inhibitor. There are five triterpenoids identified in C.decandra leaves used as ligand tests, including lupenone, betulin, betulonic acid, betulinic acid, and lupeol. The descriptive method was applied in this investigation. This study was carried out from June to September 2022. Based on the molecular interactions, the binding affinity of triterpenoids was lower than the native ligand and control ligand. Lupenone, lupeol, betalonic acid, and betulinic acid inhibited α-amylase activity by non-competitive inhibition. It was predicted that betulin inhibited α-amylase activity through competitive inhibition.
Phytochemical Compound Identification of Mangrove Leaves Sonneratia alba and in Silico Analysis as Antidiabetic Puspitasari, Yunita Eka; Hardoko, Hardoko; Sulistiyati, Titik Dwi
Jurnal Perikanan dan Kelautan Vol. 27 No. 2 (2022): June
Publisher : Faculty of Fisheries and Marine, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.2.241-248

Abstract

Mangrove plant, Sonneratia alba, can be found abundantly in Indonesia. Some previous studies reported phytochemical screening and bioactivity test of Sonneratia sp. as anti-diabetic (in vitro and in vivo analysis). However, phytochemical identification of S. alba leaves and inhibitory activity of α-glucosidase as anti-diabetic have not been reported. The purpose of this study are to identify phytochemical compounds of methanolic extracts S. alba leaves, and to predict inhibitory mechanisms of S. alba leaves against α-glucosidase through in silico analysis. In this study, the research method consists of two steps namely identification phytochemical compounds of methanolic extracts S. alba leaves, and prediction inhibitory activities of phytochemical compounds of methanolic extracts S. alba leaves against α-glucosidase by molecular docking (in silico) analysis. Methanolic extracts S. alba leaves contained orientin, vitexin, luteolin, oleanolic acid and reserpine. Reserpine was identified for the first time in S. alba leaves. Based on in silico analysis, binding energy of orientin, vitexin, luteolin, oleanolic acid, reserpine and acarbose (positive control) against α-glucosidase were -9,7; -9,7; -9,2; -8,6; -10,0 dan -8,3 kcal/mol. This result indicated that compounds inhibited α-glucosidase activities and can be considered as antidiabetic agent.
PELATIHAN ECO-PRINT DENGAN MEMANFAATKAN RANTING MANGROVE Rhizophora mucronata SEBAGAI PEWARNA ALAMI DI POKLAHSAR SUMBER REJEKI, NGULING, PASURUAN puspitasari, yunita eka; Puspitasari, Diah Agustina; Iyati, Wasiska; Sari, Mariyana; Putra, Aditya Pratama; Kamila, Virda; Sumarto, Tria Are; Gaol, Nanda Lumban; Nugroho, Pradipta Widyo; Rahmadhana, Anitsa
Prosiding Konferensi Nasional Pengabdian Kepada Masyarakat dan Corporate Social Responsibility (PKM-CSR) Vol 7 (2024): PKMCSR2024: Kolaborasi Hexahelix dalam Optimalisasi Potensi Pariwisata di Indonesia: A
Publisher : Asosiasi Sinergi Pengabdi dan Pemberdaya Indonesia (ASPPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37695/pkmcsr.v7i0.2339

Abstract

Ranting mangrove Rhizophora mucronata yang jatuh di area mangrove Desa Penunggul, Nguling Pasuruan hanya dibiarkan saja dan tidak dimanfaatkan lebih lanjut, meskipun diketahui ranting tersebut mengandung tannin sebesar 30% yang berpotensi digunakan sebagai bahan pewarna alami tekstil. Dalam rangka meningkatkan nilai ekonomi dari mangrove maka ranting mangrove di manfaatkan sebagai zat pewarna alami untuk kain eco print. Eco-print merupakan teknik pewarnaan dengan memanfaatkan bahan-bahan dari bagian tumbuhan yang mengandung tanin tinggi. Kegiatan pelatihan ini ditujukan untuk melatih wanita istri nelayan yang tergabung di Poklahsar Sumber Rejeki Nguling untuk membuat eco-print dengan memanfaatkan ranting daun bakau R. mucronata sebagai pewarna alami, serta menggunakan tanaman seperti daun mangrove, ranting cemara, daun jarak yang dapat ditemui dengan mudah disekitar area mangrove sebagai motif eco-print. Pelatihan ini juga bertujuan untuk mendukung Desa Penunggul sebagai desa wisata bahari Penunggul, sehingga eco-print ini menjadi salah satu produk buah tangan yang khas dari desa tersebut. Metode kegiatan pengabdian masyarakat adalah kegiatan focus group discussion (FGD), participant learning center (PCL) melalui pelatihan dan pendampingan setelah diadakan pelatihan. Berdasarkan kuisioner yang dikumpulkan selama pelatihan dan pendampingan menunjukkan bahwa wanita istri nelayan memiliki minat yang cukup baik untuk melanjutkan produksi eco-print dengan memanfaatkan ranting tanaman bakau R. mucronata sebagai pewarna alami. Hal ini disebabkan oleh mudahnya bahan yang mudah ditemukan disekitar sehingga menekan biaya produksi, selain itu teknik eco-print lebih mudah diaplikasikan dibandingkan teknik pewarnaan tekstil yang lain. Meskipun kelompok wanita ini memproduksi kain eco print dengan modal yang masih terbatas, tetapi mereka sudah sangat terampil dalam mengkombinasikan warna serta mengembangkan produksi kain eco print tidak hanya sebagai pashmina eco-print namun juga sebagai pakaian serta menjual produk tersebut pada toko online.
PELATIHAN PEMBUATAN ECO-PRINT DARI PEWARNA ALAMI LIMBAH TEPUNG BUAH MANGROVE Rhizophora mucronata DI POKLAHSAR SUMBER REJEKI, NGULING, PASURUAN PUSPITASARI, YUNITA EKA; Puspitasari, Diah Agustina; Sari, Mariyana; Nugroho, Pradipta Widyo; Rahmadhana, Anitsa; Hamijaya, Prasis Damai Nursyam
Jurnal Sinergitas PKM & CSR Vol. 7 No. 3 (2023): DECEMBER
Publisher : Universitas Pelita Harapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19166/jspc.v7i3.7767

Abstract

Selama ini, buah Rhizophora mucronata telah dimanfaatkan oleh masyarakat sekitar mangrove Desa Penunggul, Nguling, Pasuruan khususnya Poklahsar Sumber Rejeki sebagai bahan baku pembuatan tepung buah mangrove. Hasil samping dari proses penepungan buah mangrove adalah air berwarna coklat dari perendaman buah mangrove. Hasil samping ini yang digunakan sebagai bahan pewarna dalam pembuatan mangrove eco-print. Teknik eco-print ini diperkenalkan kepada Poklahsar Sumber Rejeki sebab teknik ini lebih mudah untuk dilakukan oleh orang yang masih awam serta lebih ramah lingkungan. Tujuan kegiatan pengabdian masyarakat ini yaitu untuk meningkatkan nilai tambah hasil samping pengolahan tepung buah R. mucronata serta ketrampilan kelompok wanita Poklahsar Sumber Rejeki. Metode kegiatan pengabdian masyarakat adalah kegiatan focus group discussion (FGD), participant learning center (PCL) melalui pelatihan dan participatory action (PA) pendampingan pasca pelatihan. Hasil kegiatan ini menunjukkan bahwa kelompok Poklahsar Sumber Rejeki memiliki minat yang cukup tinggi dalam kegiatan pelatihan tersebut dan memiliki keinginan untuk bisa memproduksi mangrove eco-print serta memanfaatkan air rendaman buah mangrove sebagai bahan pewarna alami, sebab lebih mudah dilakukan dan tidak membutuhkan biaya yang mahal. Dengan harapan, mangrove eco-print mangrove dapat digunakan sebagai buah tangan dari mangrove Desa Penunggul, Pasuruan.
PENGHAMBATAN α-GLUCOSIDASE OLEH FLAVONOID DARI EKSTRAK ETIL ASETAT DAUN MANGROVE Sonneratia alba SEBAGAI ANTIDIABETES MELALUI PENAMBATAN MOLEKULER Puspitasari, Yunita Eka; Tampubolon, Hezkiel Oktorully; Fajrin, Alifiah Nur; Sulistiyati, Titik Dwi; Hardoko, Hardoko
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 19, No 1 (2023): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.19.1.15-22

Abstract

α-glucosidase memiliki peran penting dalam metabolisme karbohidrat pada tubuh manusia dengan bekerja memutus rantai oligosakarida menjadi gula sederhana seperti monosakarida.Penghambatan aktivitas α-glucosidase menjadi salah satu mekanisme untuk mengendalikan kadar glukosa dalam darah penderita diabetes. Obat herbal sebagai inhibitor α-glucosidase bersumber dari bahan alami laut khususnya mangrove menarik perhatian. Buah pedada Sonneratia alba telah diketahui mengandung senyawa fitokimia yang berpotensi sebagai antidiabetes, akan tetapi belum pernah dilaporkan kandungan senyawa bioaktif yang berperan sebagai inhibitor α-glucosidase. Kelimpahan daun pada tanaman mangrove lebih tinggi daripada buah sehingga pada penelitian ini menggunakan daun sebagai penghambat aktivitas α-glucosidase. Tujuan dari penelitian ini adalah untuk menganalisa profil senyawa fitokimia flavonoid dari ekstrak etil asetat daun pedada S. alba, dan untuk menentukan senyawa fitokimia tersebut yang berperan sebagai inhibitor α-glucosidase secara in silico melalui penambatan molekuler. Hasil penelitian menunjukkan tiga senyawa fitokimia flavonoid dari ekstrak etil asetat daun pedada S. alba seperti luteolin, apigenin, dan diosmetin telah diidentifikasi. Menurut kaidah Lipinski, analisa druglikeness dan toksisitas, maka semua senyawa tersebut aman untuk dikonsumsi, Hasil penambatan molekuler menunjukkan energi ikatan yang dihasilkan dari interaksi reseptor dan ligan uji lebih rendah daripada energi ikatan dari reseptor dan ligan kontrol maupun alami. Sehingga dapat disimpulkan bahwa luteolin, apigenin dan diosmetin dari ekstrak etil asetat daun pedada berpotensi sebagai inhibitor α-glucosidase. α-glucosidase plays an important role in carbohydrate metabolism in the human body by breakdown oligosaccharides into monosaccharides. The inhibition of α-glucosidase activity is one of the mechanisms for controlling blood glucose levels in diabetes patients. Herbal drug as an α-glucosidase inhibitor from marine resources, especially mangroves is attracting interest. The fruit of Sonneratia alba contains phytochemical compounds potent as antidiabetic but no report related to their bioactive compound as inhibitor α-glucosidase. The abundance of leaves is higher than the fruit; therefore, in this study, the leaves will be used as α-glucosidase inhibitors. This study aimed to profile bioactive compounds of ethyl acetate extract of pedada S. alba leaves and to evaluate phytochemical compounds of S. alba leaves as α-glucosidase inhibitors using molecular docking in silico. The results showed that three phytochemical compounds of ethyl acetate extract of S. alba leaves, such as luteolin, apigenin, and diosmetin have been identified. According to the Lipinski’s rule, drug likeness, and toxicity test, all compounds are safe to consume. Docking studies showed that binding energy between receptor and phytochemical compounds is lower than binding energy between receptor and ligand control. In conclusion, luteolin, apigenin and diosmetin of ethyl acetate extract of pedada S. alba leaves have potent as α-glucosidase inhibitors.
IN SILICO STUDY OF TRITERPENOID IDENTIFIED FROM Ceriops decandra LEAVES AS INHIBITORS OF α-AMYLASE Puspitasari, Yunita Eka; Tambunan, Jeny Ernawati; Hardoko, Hardoko
Asian Journal of Aquatic Sciences Vol. 5 No. 3 (2022): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

α-amylase has a pivotal role in catalyzing the cleavage of α-1,4-glycosidic bonds of polysaccharides to produce oligosaccharides. The inhibition of α-amylase delays the breakdown of carbohydrates, causing a reduction of blood glucose levels absorption in diabetes patients. The exploration of α-amylase inhibitors has attracted because society assumed that utilizing herbal medicine reduced the side effect of prescribed drugs. Mangrove from genus Ceriops have been used as antidiabetic, but the mechanism as α-amylase inhibitors has not been reported. Consumption of leaves extract of C.decandra reduced blood glucose level in diabetic rats, and triterpenoids have been identified from the leaves. With this in mind, this study aims to predict the molecular interactions between α-amylase (PDB ID: 4GQR) and the inhibitors, triterpenoid identified in C.decandra leaves, and to evaluate the potency of triterpenoid as α-amylase inhibitor. There are five triterpenoids identified in C.decandra leaves used as ligand tests, including lupenone, betulin, betulonic acid, betulinic acid, and lupeol. The descriptive method was applied in this investigation. This study was carried out from June to September 2022. Based on the molecular interactions, the binding affinity of triterpenoids was lower than the native ligand and control ligand. Lupenone, lupeol, betalonic acid, and betulinic acid inhibited α-amylase activity by non-competitive inhibition. It was predicted that betulin inhibited α-amylase activity through competitive inhibition.
Phytochemical Compound Identification of Mangrove Leaves Sonneratia alba and in Silico Analysis as Antidiabetic Puspitasari, Yunita Eka; Hardoko, Hardoko; Sulistiyati, Titik Dwi; Fajrin, Alifah Nur; Tampubolon, Hezkiel Oktorully
Jurnal Perikanan dan Kelautan Vol. 27 No. 2 (2022): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Mangrove plant, Sonneratia alba, can be found abundantly in Indonesia. Some previous studies reported phytochemical screening and bioactivity test of Sonneratia sp. as anti-diabetic (in vitro and in vivo analysis). However, phytochemical identification of S. alba leaves and inhibitory activity of α-glucosidase as anti-diabetic have not been reported. The purpose of this study are to identify phytochemical compounds of methanolic extracts S. alba leaves, and to predict inhibitory mechanisms of S. alba leaves against α-glucosidase through in silico analysis. In this study, the research method consists of two steps namely identification phytochemical compounds of methanolic extracts S. alba leaves, and prediction inhibitory activities of phytochemical compounds of methanolic extracts S. alba leaves against α-glucosidase by molecular docking (in silico) analysis. Methanolic extracts S. alba leaves contained orientin, vitexin, luteolin, oleanolic acid and reserpine. Reserpine was identified for the first time in S. alba leaves. Based on in silico analysis, binding energy of orientin, vitexin, luteolin, oleanolic acid, reserpine and acarbose (positive control) against α-glucosidase were -9,7; -9,7; -9,2; -8,6; -10,0 dan -8,3 kcal/mol. This result indicated that compounds inhibited α-glucosidase activities and can be considered as antidiabetic agent.
Phytochemical Compound Identification of Mangrove Leaves Sonneratia alba and in Silico Analysis as Antidiabetic Puspitasari, Yunita Eka; Hardoko, Hardoko; Sulistiyati, Titik Dwi; Fajrin, Alifah Nur; Tampubolon, Hezkiel Oktorully
Jurnal Perikanan dan Kelautan Vol. 27 No. 2 (2022): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Mangrove plant, Sonneratia alba, can be found abundantly in Indonesia. Some previous studies reported phytochemical screening and bioactivity test of Sonneratia sp. as anti-diabetic (in vitro and in vivo analysis). However, phytochemical identification of S. alba leaves and inhibitory activity of α-glucosidase as anti-diabetic have not been reported. The purpose of this study are to identify phytochemical compounds of methanolic extracts S. alba leaves, and to predict inhibitory mechanisms of S. alba leaves against α-glucosidase through in silico analysis. In this study, the research method consists of two steps namely identification phytochemical compounds of methanolic extracts S. alba leaves, and prediction inhibitory activities of phytochemical compounds of methanolic extracts S. alba leaves against α-glucosidase by molecular docking (in silico) analysis. Methanolic extracts S. alba leaves contained orientin, vitexin, luteolin, oleanolic acid and reserpine. Reserpine was identified for the first time in S. alba leaves. Based on in silico analysis, binding energy of orientin, vitexin, luteolin, oleanolic acid, reserpine and acarbose (positive control) against α-glucosidase were -9,7; -9,7; -9,2; -8,6; -10,0 dan -8,3 kcal/mol. This result indicated that compounds inhibited α-glucosidase activities and can be considered as antidiabetic agent.