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All Journal Jurnal MIPA Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Pharmaceutical Science and Technology Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Pendidikan Biologi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioedukatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bioedukasi: Jurnal Pendidikan Biologi Jurnal Sains dan Edukasi Sains Indigenous Biologi Bioed : Jurnal Pendidikan Biologi BIOCHEPHY: Journal of Science Education Al-Khidmah Jurnal Pengabdian Masyarakat Jurnal Pengabdian Papua AGRIC Engagement: Jurnal Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia Berita Biologi Biodiversitas Journal of Biological Diversity
Sri Kasmiyati
Universitas Kristen Satya Wacana
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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EXPLORATION OF THE POTENTIAL OF ASTERACEAE FAMILI PLANTS ETHANOL EXTRACT AS BIOLARVICIDES AGAINST THIRD-INSTAR Aedes aegypti LARVAE Stefanus Agung Dwianto; Sri Kasmiyati; Elizabeth Betty Elok Kristiani
Berita Biologi Vol 25 No 1 (2026): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2026.14933

Abstract

The improper use of synthetic larvicides results in detrimental environmental impacts. To mitigate these effects, biolarvicides serve as a viable alternative. Plants within the Asteraceae family contain bioactive compounds with potential biolarvicidal properties. This research aimed to explore the potential of Asteraceae species as biolarvicides. This research utilizes Artemisia vulgaris, Cosmos caudatus, Eclipta prostrata, and Tagetes erecta. Extraction was conducted by the maceration method using 96% ethanol. The resulting extracts were analyzed for secondary metabolite content. Data were analyzed using SAS 9.0 software Two-way ANOVA, followed by Tukey’s post-hoc test. Larvicidal bioassays were performed on third-instar Aedes aegypti larvae at concentrations of 1,000-10,000 ppm. The parameters measured included mortality percentage, LC50, and LC90. Morphological changes in dead larvae were observed and compared against a control group. The results indicated that secondary metabolite in the leaf ethanolic extracts were higher than those in the stems and roots. The leaf ethanolic extract of C. caudatus exhibited the highest content of flavonoids (928.3±368.7 mg/g), phenolics (310.2±84.9 mg/g), tannins (187.1± 55.5 mg/g), and saponins (314±95.4 mg/g extract). Conversely, the highest alkaloid content was recorded in the leaf extract of T. erecta (27.8±7.1 mg/g). The highest mortality rate was observed in larvae exposed to A. vulgaris leaf extract, reaching 100% mortality across all concentrations within 24 hours. Consequently, LC50 and LC90 values for A. vulgaris could not be determined. For C. caudatus, the LC50 values were 7204.7 ppm (24 h) and 6839.7 ppm (48 h), while LC90 values were 9077.3 ppm (24 h) 8226.4 ppm (48 h), respectively. E. prostrata yielded an LC50 of 10255.6 ppm and an LC90 of 18048.9 ppm at 24 hours; however, these values were not detectable at 48 hours. T. erecta showed an LC50 of 6569.6 ppm (24 h) and 6397.1 ppm (48 h), with LC50 values of 9682.1 ppm and 9343.3 ppm, respectively. Morphological observations of dead larvae exposed the extracts alterations, including pigmentation, elongation, and shrinkage. While the leaf ethanolic extract of C. caudatus contained the highest concentration of secondary metabolites. The leaf ethanolic extract of A. vulgaris demonstrated the greatest potential as a biolarvicide.
Peran Teknologi Nanopartikel Pada Budidaya, Produksi, dan Pemanfaatan Senyawa Tanaman Vanili (Vanilla planifolia) Audri Septina Putri Rimanti; Dani Karel Marthin Djurubasa; Sri Kasmiyati; Elizabeth Betty Elok Kristiani
Buletin Anatomi dan Fisiologi Volume 10, Nomor 2, Tahun 2025
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.10.2.2025.102-111

Abstract

Aplikasi nanoteknologi, di dunia pertanian maupun kesehatan, kini semakin marak untuk dikembangkan. Vanilla planifolia atau tanaman vanili, yang merupakan penghasil senyawa vanilin, memiliki potensi pengaplikasian nanoteknologi. Peningkatan pertumbuhan tanaman dengan menggunakan teknologi nanofertilizer dan peningkatan senyawa metabolit sekunder vanilin dengan menggunakan teknologi nanoelisitor dapat memberikan hasil maksimal bagi panen tanaman vanili. Disisi lain, vanilin yang merupakan senyawa aktif pada tanaman vanili juga memiliki khasiat sebagai obat dan bahkan dapat dimanfaatkan dalam bidang kecantikan. Penelitian ini bertujuan untuk menggali peran nanoteknologi yang memiliki potensi pengaplikasiannya pada tanaman vanili, baik pada budidaya tanaman maupun pada pemanfaatan senyawa vanilin dalam bidang kesehatan dan kecantikan. Dari hasil yang didapatkan, nanoteknologi dalam bentuk nanofertilizer memiliki potensi besar dalam meningkatkan pertumbuhan tanaman vanili serta dapat membantu tanaman dalam menghadapi cekaman biotik dan abiotik yang mengganggu pertumbuhannya. Nanoteknologi juga dapat diaplikasikan pada senyawa vanilin, dimana vanilin dalam bentuk nanopartikel berperan sebagai anti-inflamasi, anti-kanker, anti-oksidan, serta berperan sebagai senyawa pengikat alami yang mendukung penyembuhan luka. Kemampuan tersebut dapat dimanfaatkan sebagai nanoteknologi dalam bidang kesehatan serta bidang kecantikan  Applications of nanotechnology, in the agriculture and health sector, are now increasingly being developed. Vanilla planifolia or vanilla plants, which are producers of vanillin compounds, have the potential for nanotechnology applications. Increasing plant growth using nanofertilizer technology and increasing vanillin secondary metabolite compounds using nanoelicitor technology can provide maximum yield for vanilla plant. On the other hand, vanillin, which is the active compound in the vanilla plant, also has medicinal properties and can even be used in the field of beauty. This research aims to explore the role of nanotechnology that has the potential for application in vanilla plants, both in plant cultivation and in the use of vanillin compounds in the field of health and beauty. From the results obtained, nanotechnology in the form of nanofertilizer has great potential in increasing the growth of vanilla plants and can help plants in dealing with biotic and abiotic stress that interferes with their growth. Nanotechnology can also be applied to vanillin compounds, where vanillin in the form of nanoparticles acts as an anti-inflammatory, anti-cancer, anti-oxidant, and also acts as a natural binding compound that supports wound healing. This ability can be used as nanotechnology in the health and beauty fields 
Exploring anticancer activity of wild and polyploid mutant of Artemisia cina Sri Kasmiyati; Elizabeth Betty Elok Kristiani; Maria Marina Herawati; Ferdy S Rondonuwu
Biodiversitas Journal of Biological Diversity Vol. 22 No. 3 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220319

Abstract

Abstract. Kasmiyati S, Kristiani EBE, Herawati MM, Rondonuwu FS. 2021. Exploring anticancer activity of wild and polyploid mutant of Artemisia cina.  Biodiversitas 22: 1227-1234. The research aims were to explore the anticancer compounds in wild type (W) and polyploid mutant (P) of Artemisia cina Berg ex Poljakov using NIR and compare their cytotoxicity and selectivity on WiDR colon cancer and HTB183 lung cancer cell lines and Vero normal cell line. The W was obtained from B2P2TOOT Tawangmangu, Indonesia while P was by inducing A. cina shoot culture with 100 mg/l colchicine for 48 hours. They were extracted with hexane using the soxhletation method for 6 hours. The anticancer compounds were detected using NIR. The cytotoxic activity was determined using MTT assay, with Doxorubicin (D) as a control. The calculation of IC50 value used SPSS16 with a Probit analysis. The P contained the determined four compounds while the W contained no rutin. The IC50 values of W, P, and D were 295.5, 84.1, and 0.5 µg/mL on WiDr, 322.4, 128.6, and 39.9 µg/mL on HTB 183, whereas on Vero were 104, 315.6, and 295.5 µg/mL respectively. The selectivity indexes of W, P, and D were 34, 4, and 91 on WiDr, while HTB 183 was 31, 3, and 7, respectively. The P contained artemisinin, quercetin, kaemferol, and rutin, while the W contained no rutin. The cytotoxicity of both plants was less than doxorubicin. Both plants were selective on WiDR and HTB 183.
The cytotoxic and apoptotic effects of wild and polyploidy genotype of Artemisia cina extracts on the WiDr colon and HTB-183 lung cancer cell lines Elizabeth Kristiani; Sri Kasmiyati; Maria Herawati
Biodiversitas Journal of Biological Diversity Vol. 22 No. 7 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220735

Abstract

Abstract. Kristiani EBE, Kasmiyati S, Herawati MM. 2021. The cytotoxic and apoptotic effects of wild and polyploidy genotype of Artemisia cina extracts on the WiDr colon and HTB-183 lung cancer cell lines. Biodiversitas 22: 2844-2852. Artemisia belongs to the Asteraceae family, usually used for traditional treatments of various diseases in China. Artemisia cina Berg ex Poljakov, which lots found in Indonesia, has not been studied much like a cancer drug. The research aims were (i) to compare the bioactive content of hexane and ethyl acetate extract of wild (TWN and KJT) and polyploidy type (J and M) of A. cina and its cytotoxicity on WiDr and HTB-183 cancer cells, (ii) to evaluate the cytotoxicity mechanism of the most substantial extract of it. All of the extracts were prepared by maceration methods using hexane and ethyl acetate separately. The research method used was quantitative with experimental design. The determination of quercetin, kaempferol, and artemisinin used HPLC. The cytotoxicity of the extract determined using the MTT method. The assay of the specific protein related to apoptosis using ICC assay. The content of three bioactive compounds on ethyl acetate extracts higher than hexane extract, in which the extract of J and KJT genotypes had higher levels than M and TWN genotypes. The IC50 value (µg/mL) of extracts against cancer cell lines tested was about 400-700 hexane extracts and 200-500 ethyl acetate extracts. The BCl-2 expressions of both cancer cell lines decreased by treating TWN-EA and M-EA, while the P53 expressions increased. The TWN-EA induced apoptosis of HTB-183 cell lines through caspase-8 and caspase-9 pathways, but M-EA induced caspase-9 pathway only. The TWN and M genotypes were potential to use as anticancer agents on colon and lung cancer.
Isolation and identification of Cr-resistant bacteria from the rhizosphere of Tagetes sp. and their ability to reduce Cr(VI) VINCENTIA IRENE MEITINIARTI; ELSA KRISTIANI PUTRI; ATHALIA EUGENIA RUNTU; RULLY ADI NUGROHO; SRI KASMIYATI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 8 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230832

Abstract

Abstract. Meitiniarti VI, Putri EK, Runtu AE, Nugroho RA, Kasmiyati S. 2022. Isolation and identification of Cr-resistant bacteria from the rhizosphere of Tagetes sp. and their ability to reduce Cr(VI). Biodiversitas 23: 4118-4124. Bioremediation involving the interaction of plants with microorganisms is a technique for improving the environment polluted with chromium hexavalent [Cr(VI)]. Marigolds (Tagetes sp.) are known to be a hyperaccumulator plant for Cr(VI) and there are microbes in its rhizosphere that enable the plant tolerant to heavy metals. The aims of this study were to isolate and identify indigenous rhizobacteria resistant to Cr(VI) from Tagetes sp. and to test the ability of these bacterial isolates to reduce Cr(VI). Three isolates of Cr(VI) resistant bacteria were obtained in this study, namely Micrococcus luteus RT-9, Stenotrophomonas maltophilia RT-12, and Brevundimonas sp. RT-23. M. luteus RT-9 and S. maltophilia RT-12 which were resistant to Cr(VI) 100 mg L-1 and their mixture were tested for their ability to reduce Cr(VI) in a liquid medium. Microbacterium sp. SpR3 obtained by the previous study was used as a reference. The results showed that the isolate of S. maltophilia RT-12 had a higher ability (2.2 mg L-1 h-1) than M. luteus RT-9 (0.9 mg L-1 h-1). S. maltophilia RT-12 had the ability to reduce Cr(VI) similar to Microbacterium sp. SpR3. The mixed inoculation of M. luteus RT-9, S. maltophilia RT-12, and Microbacterium sp. SpR3 did not show a difference in the reduction ability of Cr (VI) compared to pure cultures.
Resistance selection of local sorghum varieties in East Nusa Tenggara, Indonesia against Rhizoctonia solani EWINDA ISENSI FENI; SRI KASMIYATI; V. IRENE MEITINIARTI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 10 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241012

Abstract

Abstract. Feni EI, Kasmiyati S, Meitiniarti VI. 2023. Resistance selection of local sorghum varieties in East Nusa Tenggara, Indonesia against Rhizoctonia solani. Biodiversitas 24: 5309-5318. Sorghum is a multifunctional crop that can be used as an alternative food for the people of East Nusa Tenggara. However, one of the challenges in cultivating sorghum in this region is the presence of the Rhizoctonia solani pathogen. This fungus causes a devastating root rot disease in sorghum crops, resulting in loss of crop yields and potentially disrupting food security in the region. Using resistant varieties is one of the most effective ways to prevent the growth of R. solani biologically. Therefore, it is important to study the resistance of local sorghum varieties. The purpose of this study was to determine different sorghum varieties' resistance responses to the R. solani fungus that causes root rot disease. This study used a completely randomized design (CRD) with 2 levels of treatment: the treatment of sorghum varieties and the treatment of fungi inoculums, which were inoculated separately and simultaneously. Each treatment was repeated 3 times. Observations and data collection were carried out on the 7th day, and some of the parameters observed included germination percentage, radicle length, coleoptile length, sprout length, GTI value, and disease incidence value. The sorghum variety with the highest resistance value was found in the red variety. This red variety had a disease incidence value of 63.3%, much lower than the other four test varieties. These results provide important insights for developing a more root rot disease-resistant local sorghum plant variety.
Interaction between Eichornia crassipes with Microbacterium sp. SpR3 and Micrococcus luteus RT-9 in Cr6+ reduction in liquid media ANDRIYANI DEA WULANDARI; V. IRENE MEITINIARTI; SRI KASMIYATI
Biodiversitas Journal of Biological Diversity Vol. 25 No. 3 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250307

Abstract

Abstract. Wulandari AD, Meitiniarti VI, Kasmiyati S. 2024. Interaction between Eichornia crassipes with Microbacterium sp. SpR3 and Micrococcus luteus RT-9 in Cr6+ reduction in liquid media. Biodiversitas 25: 957-963. Water Pollution caused by Cr6+ contamination is difficult to remove from the environment. One of the efforts to reduce Cr6+ contamination in water is by bioremediation. Bioremediation using the interaction of rhizosphere bacteria with aquatic plants can be employed to decrease the content of Cr6+ in water environment. This study aimed to determine the effect of adding Microbacterium sp. SpR3, Micrococcus luteus RT-9, and a mixture of both bacteria on the ability of Eichornia crassipes to reduce the concentration of Cr6+ in the growth media and the plant's growth potential. This study was conducted in the Microbiology Laboratory of the Faculty of Biology at Satya Wacana Christian University. Eichornia crassipes plans of similar size were grown in 450 mL of Hoaglands solution with Cr6+ 5 mg.L-1 in a container. The treatment in this study involves the addition of 50 mL (10% of the working volume) of Microbacterium sp SpR3 culture, Micrococcus luteus RT- 9, and a combination of both Cr6+-reducing bacteria. The reduction rate of Cr6+ concentration in the mixture of both types of bacteria reached 88.06%, which was higher compared to the control without the addition of the inoculum (87.08%). The inoculation of both Cr6+ reducing bacteria did not hibit the growth rate of the plants. The ability of Microbacterium sp. SpR3 and Micrococcus luteus RT-9 to reduce Cr6+ in liquid media can be employed in the bioremediation of Cr6+ contaminated wastewater.
Identification and production of indole-3-acetic acid by bacteria isolated from eco-enzymes V. IRENE MEITINIARTI; SRI KASMIYATI; EZRA EZRA; RULLY ADI NUGROHO; AGNA S. KRAVE
Biodiversitas Journal of Biological Diversity Vol. 26 No. 1 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260112

Abstract

Abstract. Meitiniarti VI, Kasmiyati S, Nugroho RA, Krave AS. 2025. Identification and production of indole-3-acetic acid by bacteria isolated from eco-enzymes. Biodiversitas 26: 111-117. Phytohormone-producing microorganisms are an essential component of biofertilizers. One example of a phytohormone is Indole Acetic Acid (IAA). IAA-producing microorganisms can be originated from various habitats. In this study, IAA-producing bacteria will be isolated from eco-enzyme, a liquid-fermented organic material rich in benefits and contains numerous microorganisms and IAA. The research involves processes of isolation, detection of cell and IAA production, and molecular identification. Through the processes of isolation and purification, 14 bacterial isolates were obtained. After testing their ability to produce IAA using a medium containing L-tryptophan and Salkowski's reagent, only 11 isolates were found to produce IAA. The DNA of these 11 isolates was isolated, amplified, sequenced, and identified through molecular analysis. The nucleotide sequences of these 11 bacterial isolates have been registered in the gene bank and assigned accession numbers PQ095569 to PQ095579. Based on alignment and phylogenetic tree analysis, the 11 isolates were grouped into three categories: the Bacillus group, consisting of Bacillus altitudinis, Bacillus subtilis, Bacillus licheniformis, Priestia megaterium, and Paenibacillus sp.; lactic acid bacteria, including Lacticaseibacillus paracasei and Lactiplantibacillus plantarum; and vibrio-shaped bacteria, including Vibrio sp. and Vibrio diazotrophicus. The Bacillus group (including Paenibacillus megaterium) could produce high levels of IAA. However, among the members of this group, P. megaterium exhibited the highest cell production capability and IAA production, with values of 2982.208 mg·L?¹ and 35.49 mg·L?¹, respectively. This high growth ability and IAA production make P. megaterium a promising candidate as an inoculum for use as a PGPR (Plant Growth-Promoting Rhizobacterium).
Enhancing Bird’s Eye Chili (Capsicum frutescens) Productivity Through Combined Application of Eco-Enzyme and Trichoderma viride Sheina Revania Putri Doo; Sri Kasmiyati; Samse Pandiangan; Siti Nur Hidayati; V. Irene Meitiniarti
Biosaintifika: Journal of Biology & Biology Education Vol. 18 No. 2 (2026): August 2026
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v18i2.30020

Abstract

Bird’s eye chili (Capsicum frutescens L.) is a high-value horticultural crop, yet yields in Indonesia remain low due to long-term use of chemical fertilizers, which reduce soil fertility. Organic fertilizer alternatives, such as eco-enzyme and Trichoderma viride, offer potential for sustainable agriculture. This research aimed to study the impact of eco-enzyme and T. viride, applied individually and in combination, on the growth and yield of Bird’s eye chili, as well as on soil N, P, K, organic carbon, and IAA levels. The study employed a Completely Randomized Design (CRD) with four treatments: control, eco-enzyme, T. viride, and a combination of eco-enzyme and T. viride, each with eight replications. The research was conducted in a polybag over 20 weeks. Parameters observed were plant height, leaf number, root length, number of flowers and fruits, fruit weight, and soil N, P, K, C-organic, and IAA. Compared with the control treatment, the application of eco-enzyme and T. viride improved several growth and soil quality parameters. The combined treatment increased vegetative growth and enhanced soil N, P, K, and IAA contents, whereas T. viride alone produced the highest fruit yield. These results suggest that eco-enzyme and T. viride can be used as biological inputs to improve chili production while helping maintain soil fertility and reduce dependence on chemical fertilizers. The findings are relevant to SDG 2 (Zero Hunger), SDG 12 (Responsible Consumption and Production), and SDG 15 (Life on Land), particularly regarding sustainable food production, resource recycling, and soil conservation.
PEMBERDAYAAN SISWA SMA SALATIGA UNTUK MELESTARIKAN KEARIFAN LOKAL MELALUI INOVASI MINUMAN JAMU DAN HERBAL KEKINIAN Elizabeth Betty Elok Kristiani; Desti Christian Cahyaningrum; Natalia Rosa Keliat; Sri Kasmiyati; Desi Fajar Priyayi; Rully Adi Nugroho; Vincentia Irene Meitiniarti; Agna Sulis Krave
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol. 3 No. 1 (2022): Jurnal Abdikmas
Publisher : LPPM Universitas Kahuripan Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Telah dilakukan kegiatan Pengabdian kepada Masyarakat berupa pendampingan siswa SMA Kota Salatiga. Kegiatan bertujuan untuk peningkatan pengetahuan dan minat generasi muda terhadap jamu melalui inovasi Minuman Jamu Herbal kekinian guna melestarikan kearifan lokal jamu. Siswa SMA di Kota Salatiga sebagai mitra kegiatan mewakili generasi muda. Kegiatan terdiri dari penggalian pengetahuan mitra tentang Minuman Jamu dan Herbal (MJH), sosialisasi tentang pemanfaatan MJH sebagai upaya meningkatkan imunitas tubuh, sosialisasi tentang mengembangkan peluang bisnis MJH; Pendampingan mitra dalam praktik inovasi produk MJH kekinian; dan (5) Evaluasi kegiatan. Sebelum pendampingan Sebagian besar siswa mitratidak terlalu paham dan berminat terhadap produk jamu. Pendampingan berhasil peningkatan pengetahuan dan minat generasi muda terhadap jamu. Siswa mitra sangat kreatif dan inovatif dalam menciptakan produk Minuman Jamu Herbal kekinian yang menarik generasi muda antara lain minuman segar masa kini, minuman krim, pudding, jelly, permen, minuman dalam bentuk serbuk, dan es krim
Co-Authors ,, Djohan Adi Nugroho AGNA S. KRAVE Agna S. Krave Agna Sulis Krave Agustien Sri Noerwahju Ajeng Setya Magdalena Alberthnico, Ferdian Andreas A. Sukmana Andreas B.A. Sukmana Andreas Binar Aji Sukmana Andreas Binar Aji Sukmana ANDRIYANI DEA WULANDARI Anggara Mahardika Anna Helena Sabandar Ariyanto, Prasetyo Eko ATHALIA EUGENIA RUNTU Audri Septina Putri Rimanti Betty E. Kristiani Bety E.K, Elizabeth Budhi Kristianto Cantika Fanny Nurwahidah Cantika Glodia Ciputra S. Mangesa Cocok A.M. Berutu Dani Karel Marthin Djurubasa Debora Kristiani Rahardjo Desi Fajar Priyayi Desti C. Cahyaningrum Desti Christian Cahyaningrum Desti Christian Cahyaningrum Dewi Prabawaningrum Djohan Djohan Djohan Djohan Easter G. Tuandali Egarani, Grahita Rezhi Elizabeth B.E Kristiani Elizabeth B.E. Kristiani Elizabeth Betty Elok Elizabeth Betty Elok Kristiani Elizabeth Betty Elok Kristiani Elizabeth Kristiani ELSA KRISTIANI PUTRI Emma Sharon Admatha Kaya Erwien Christianto Esty Vicariat Siahaya Evangs Mailoa EWINDA ISENSI FENI EZRA EZRA Fathiya Ulfa Nurrasyida Ferdy Semuel Rondonuwu Gabriella L. Mataradja Hanita Yulia Hindriyanto Dwi Purnomo Ie, Johanes Juan Yutama Putra Imel T.Y. Zebua Irfan Dwidja Priyambada Irfan Dwidja Priyambada, Irfan Dwidja Jessica Ayu Trisianna Trisianna Juliana Sarioa Kapitarauw, Agnesya Giovani Putri Cendana Krismiyati Kumala Dewi Kumala Dewi Lani, Rischa Karmila Laurentius Hartanto Nugroho Marchella G. Putricia Margareta Prameswati Maria Herawati Maria Marina Herawati Marisa Christina Tapilouw Nabila, Nabila Natalia R. Keliat Natalia Rosa Keliat Novellasari, Firdiana Olive Hapsari Sekar Widiantari Otilda Fatima Araujo Otilda Fátima Araújo Otilda Fatima Araujo Penina Intansari Prasgi, Henokh Christian Pratama, Dimas Seno Bagus Purwoko, Agus Putra Ie, Johanes Juan Yutama Putri Indrianasari Putri, A C. Radius Tanone Rahardja, P M. Ramagita, Marsahanda Astri Rimanti, Audri Septina Putri Rintawati Sandradewi Rintawati Sandradewi, Rintawati Risky H. Poluan Risya P. Situmorang Risya Pramana Situmorang Rully A. Nugroho Rully Adi Nugroho Sabandar, Anna Helena Samse Pandiangan Santosa S, Santosa Santosa Santosa Satria Dharmawan Kustia Dewa Seleiman, Mahmoud F. Shabrina R.A. Fernanda Sheina Revania Putri Doo Shinta, Damaris Clara Siahaya, Esty Vicariat Siti Nur Hidayati Stefanus Agung Dwianto Sucahyo Sucahyo Sucahyo Susanti P. Hastuti Susanti Pudji Hastuti Susanti Puji Hastuti Syuhuud Arumbinang Wajdi Tambunan, Roslinda Kristin Teguh I. Bayu Vicharisti Monica Mangudis Vincentia Irene Meitiniarti Vini Kurnia Ramadhani Vivian Yolanda Halim Wahyu Hari Kristiyanto Wartanto, Jokosulistyo Widyaningrum, Indy Yohanes Martono Yosephine Dita Apriliana