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All Journal METAMORFOSA Journal of Biological Sciences Jurnal MIPA Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Pendidikan Biologi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioedukatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bioedukasi: Jurnal Pendidikan Biologi Jurnal Sains dan Edukasi Sains Indigenous Biologi Proceeding Biology Education Conference Bioed : Jurnal Pendidikan Biologi BIOCHEPHY: Journal of Science Education Al-Khidmah Jurnal Pengabdian Masyarakat AGRIC Engagement: Jurnal Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia
Sri Kasmiyati
Universitas Kristen Satya Wacana
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Perbandingan Kekuatan Antibakteri Minyak Atsiri dari Tiga Varietas Jahe (Zingiber officinale Rosc) Terhadap Baccillus subtilis dan Pseudomonas aeruginosa Kristiani, Elizabeth Betty Elok; Kaya, Emma Sharon Admatha; Kasmiyati, Sri
Buletin Anatomi dan Fisiologi Volume 11, Nomor 1, Tahun 2026
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.1.1.2026.9-15

Abstract

Jahe (Zingiber officinale) merupakan tanaman rempah yang banyak tumbuh di Indonesia dan diketahui memiliki potensi sebagai agen antibakteri. Penelitian ini bertujuan untuk membandingkan aktivitas antibakteri minyak atsiri dari tiga varietas jahe yaitu jahe gajah (Z. officinale var. Roscoe), jahe merah (Z. officinale var Rubrum), dan jahe emprit (Z. officinale var Amarum)  terhadap bakteri Bacillus subtilis dan Pseudomonas aeruginosa. Ekstraksi minyak atsiri menggunakan teknik sohkletasi dengan pelarut etanol. Uji aktivitas antibakteri menggunakan metode difusi cakram. Rendemen minyak atsiri tertinggi Z. officinale var Rubrum 23,45 ± 3,32 %, diikuti Z. officinale var Amarum 19,90 ± 2,17 % dan terendah Z. officinale var. Roscoe 15,70 ± 2,42 %. Pada semua konsentrasi uji, kemampuan minyak atsiri jahe dalam menghambat pertumbuhan Baccillus subtilis lebih kecil secara signifikan dibandingkan tetrasiklin dalam tetapi lebih besar pada P. aeruginosa. Hasil uji antibakteri minyak atsiri semua tiga varietas jahe pada konsentrasi 62,6 – 1000 µg/mL terbukti menghambat pertumbuhan kedua bakteru uji dalam kategori kuat yaitu diamter daya hambat > 20 mm. Kekuatan aktivitas antibakteri minyak atsiti terhadap B. Subtilis lebih rendah dari antibiotika standar tetrasiklin, sedangkan terhadap P. aeruginosa lebih kuat. Minyak atsiri ketiga variets berpotensi untuk dijadikan sebagai agen antibakteri terhadap strain gram negatif dan gram positif.  Ginger (Zingiber officinale) is a spice plant that grows widely in Indonesia and is known to have potential as an antibacterial agent. This study aims to compare the antibacterial activity of essential oils from three varieties of ginger, namely elephant ginger (Z. officinale var. Roscoe), red ginger (Z. officinale var Rubrum), and emprit ginger (Z. officinale var Amarum) against Bacillus subtilis and Pseudomonas aeruginosa bacteria. Essential oil extraction was performed using the Soxhlet extraction technique with ethanol as the solvent. Antibacterial activity was tested using the disk diffusion method. The highest essential oil yield was obtained from Z. officinale var. Rubrum at 23.45 ± 3.32%, followed by Z. officinale var. Amarum at 19.90 ± 2.17% and the lowest from Z. officinale var. Roscoe at 15.70 ± 2.42%. At all test concentrations, the ability of ginger essential oil to inhibit the growth of Bacillus subtilis was significantly lower than that of tetracycline, but higher than that of P. aeruginosa. The antibacterial test results of essential oils from all three ginger varieties at concentrations of 62.6–1000 µg/mL proved to inhibit the growth of both test bacteria in the strong category, namely with an inhibition diameter > 20 mm. The strength of the antibacterial activity of essential oils against B. subtilis was lower than the standard antibiotic tetracycline, while against P. aeruginosa it was stronger. The essential oils of the three varieties have the potential to be used as antibacterial agents against gram-negative and gram-positive strains.
Co-Authors ,, Djohan Agna Sulis Krave Agustien Sri Noerwahju Alberthnico, Ferdian Andreas Binar Aji Sukmana Anggara Mahardika Anna Helena Sabandar Araujo, Otilda Fatima Ariyanto, Prasetyo Eko Betty Elok, Elizabeth Cahyaningrum, Desti Christian Cantika Glodia Debora Kristiani Rahardjo Desti Christian Cahyaningrum Dewi Prabawaningrum Djohan Djohan Djohan Djohan Djurubasa, Dani Karel Marthin Egarani, Grahita Rezhi Elizabeth B.E Kristiani Elizabeth Betty Elok Kristiani Elizabeth Bety E.K Fanny Nurwahidah, Cantika Fathiya Ulfa Nurrasyida Fatima Araujo, Otilda Gabriella L. Mataradja Hapsari Sekar Widiantari, Olive Ie, Johanes Juan Yutama Putra Indrianasari, Putri Irfan Dwidja Priyambada, Irfan Dwidja Jokosulistyo Wartanto Juliana Sarioa Kachingwe, Baxter Hepburn Kapitarauw, Agnesya Giovani Putri Cendana Kaya, Emma Sharon Admatha Keliat, Natalia Rosa Kumala Dewi L. Hartanto Nugroho Lani, Rischa Karmila Margareta Prameswati Maria Marina Herawati Monica Mangudis, Vicharisti Nabila, Nabila Natalia Rosa Keliat Novellasari, Firdiana Penina Intansari Prasgi, Henokh Christian Pratama, Dimas Seno Bagus Priyayi, Desi Fajar Purwoko, Agus Putra Ie, Johanes Juan Yutama Putri, A C. Rahardja, P M. Ramagita, Marsahanda Astri Rimanti, Audri Septina Putri Rintawati Sandradewi, Rintawati Risya Pramana Situmorang Rully Adi Nugroho Sabandar, Anna Helena Santosa S, Santosa Satria Dharmawan Kustia Dewa Seleiman, Mahmoud F. Setya Magdalena, Ajeng Shinta, Damaris Clara Siahaya, Esty Vicariat Sucahyo Sucahyo Sucahyo Susanti Pudji Hastuti Susanti Puji Hastuti Syuhuud Arumbinang Wajdi Tambunan, Roslinda Kristin Trisianna, Jessica Ayu Trisianna Vincentia Irene Meitiniarti Vini Kurnia Ramadhani Wahyu Hari Kristiyanto Widyaningrum, Indy Yohanes Martono Yolanda Halim, Vivian