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Effect of Induced Polyploidy on Plant Growth, Chlorophyll and Flavonoid Content of Artemisia cina Sri Kasmiyati; Elizabeth Betty Elok Kristiani; Maria Marina Herawati
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22548

Abstract

Artemisia cina is one of a member of genus Artemisia that has potential as a medicinal plant. However, the levels of Artemisia medicinal bioactive compounds are very low. Polyploidization is an alternative method that can enhance of growth and secondary metabolite productions of plants. The aims of this research were to determine the effect of polyploid induction using colchicine and plant growth regulator toward plant growth, the chlorophyll, kaemferol and quercetin contents of A. cina. Four different A. cina used in this research consisted of two diploid genotypes (TWN and KJT) and two polyploid genotypes (J and M). Induction of mutant polyploid was conducted using colchicine and combination of plant growth regulator benziladenyl (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D). The measured plant growth parameters were dry weight, leaf area, and plant height. The chlorophyll content of leaves was determined spectrophotometrically, and flavonoid content determined using HPLC. The result showed that the polyploid genotype (M) significantly decreased leaf and root growth compared to the KJT and TWN. In A. cina plants, polyploidization using colchicine is more effective in increasing the biomass than using combination plant growth regulator BA and 2,4-D. The flavonoid content of KJT was the lowest, and significantly different compared to the other plants. This study provides new information about the effect of polyploid on growth and flavonoid content in A. cina. This can be  useful information to develop A. cina to become a medicinal plant.
DETEKSI CEKAMAN OKSIDATIF AKIBAT TOKSISITAS KROM PADASonchus oleraceus L. MELALUI PENENTUAN SPESIES OKSIGEN REAKTIF SECARA SPEKTROFOTOMETRI DAN HISTOKIMIA Sri Kasmiyati; - Sucahyo
Agric Vol. 26 No. 1 (2014)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2014.v26.i1.p85-98

Abstract

Increased production of reactive oxygen species or ROS is one of the common responses to a wide range of biotic and abiotic stresses. Increased production of ROS is outstripping endogenous antioxidant defense systems has been referred to as oxidative stress. Heavy metals are known to initiate ROS generation which is implicated as a oxidative stress. Cr is a toxic heavy metal that can generate ROS like H2O2 and O2 - which cause oxidative stress. In this study, chromium toxicity was studied to detect the oxidative stress on Sonchus oleraceus weed plants by the detection of superoxide anion and H2O2. Superoxide anion was detected by staining techniques with nitroblue tetrazolium (NBT) and hydrogen peroxide by Diaminobenzidine tetrahydrochloride (DAB) staining. Results indicated that the plants were grown in sand media generate the highest (0.89 A/g FW and 3.23 mol/g FW) than in soil media (0.23 A/g FW and 2.11 mol/g FW) superoxide anion (*O- 2) and H2O2 and soil containing textile sludge (0.18 A/g FW and 2.66 mol/g FW), respectively. At application of 10 mg Cr6+/L and 250 mg Cr3+/L, the production of *O- 2 and H2O2 in leaves of sonchus plants were significantly increased compared with the control plants. The highest production of H2O2 and *O- 2 were showed in the leaves of sonchus plants grown in sand media with Cr6+ application. In this study, either Cr3+ or Cr6+ caused oxidative stress in Sonchus oleraceus weed plants. The result also showed that sonchus plants esposed to toxic Cr can suffer from oxidative stress leading to reduction of its fresh and dry plants biomass. NBT and DAB in an appropriate probe and significant value for monitoring the formation of *O- 2 and H2O2 in plants.
SKRINING FITOKIMIA DAN AKTIVITAS ANTIBAKTERI in vitro EKSTRAK HEKSANA-PETROLEUM ETER Artemisia cina Berg. ex Poljakov Elizabeth B.E Kristiani; Sri Kasmiyati; Maria Marina Herawati
Agric Vol. 27 No. 1 (2015)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2015.v27.i1.p30-37

Abstract

The objectives of this reserch were to determine the antibacterial activity of hexane-petroleum ether (1 : 1 v/v) extract of Artemisia cinaBerg. ex Poljakov on Escherichia coli and Staphylococcus aureus and its compounds. The design of the research was using completely Randomized Design in five concentration of extract that were 0, 50, 75, 100, 150, dan 200 mg/ml with four repliceted. Ethanol 60% was used as negative control and tetracycline as positive control. There were three steps of research. First step was extracted the plant using soxhlet method with hexane-petroleum ether (1:1 v/v). Second step was determined the antibacterial activity of hexane-petroleum ether (1:1 v/v) extract in various concentration of that extracts on E. Coli and S. aureus using agar diffusion method. Analysis of Variance (ANOVA) and was used to determined the significan different of diameter of inhibition between the treatments. Thirdsteps was phytochemical analysis of extract. The highest antibactrial activity on E. Coli was at 100 mg/ml whereas on S. Aureus at 150 mg/ml. That extract was contained flavonoid, alkaloid, essential oils, saponin, sterol, tritepene, hydrolized tannin, and coumarin.
Efek Interaksi Sulfate (SO42-) dan Kromium (CrO42-) terhadap Pertumbuhan dan Akumulasi Cr6+ pada Tagetes erecta L. Sri Kasmiyati; Gabriella L. Mataradja
Agric Vol. 35 No. 1 (2023)
Publisher : Fakultas Pertanian dan Bisnis, Universitas Kristen Satya Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/agric.2023.v35.i1.p45-60

Abstract

Chromium is a heavy metal that is toxic, bioaccumulative, persistent, and cannot be decomposed in the environment. The Cr6+ ion is a chromium ion that has the highest toxicity among other chromium ions, so its presence in the environment is hazardous for plants, animals, and humans. Sulfur is an essential element for plants, whose presence can reduce Cr6+ concentrations in the terrestrial environment by inhibiting the uptake of Cr6+ by plants. Tagetes erecta is a bioremediation agent plant that can remediate land polluted with Cr6+ waste. In this research, we studied the growth of T. erecta in media polluted with Cr6+ and sulfate and its ability to accumulate of Cr6+. Several parameters observe in this experiment, i.e. growth parameters, namely plant height, number of leaves, number of flowers measured at the end of the study, dry weight, root length, and Cr6+ concentration estimated using the diphenylcarbasid method. The results showed that the plant height of T. erecta. was not significantly different, and sulfate addition did not affect plant height. The addition of sulfate affected the number of leaves and flowers, dry weight of root shoots, root length, and Cr6+ content in T. erecta. polluted with Cr6+. so that in these parameters, there was a significant difference in the addition of Cr6+ and sulfate on growth of T. erecta and its ability to Cr accumulation.
Inventarisasi Keanekaragaman Vegetasi Pohon yang Dapat Mengkonservasi Air di Kawasan Sumber Mata Air Senjoyo Desti Christian Cahyaningrum; Sri Kasmiyati; Cantika Glodia
Jurnal Sains dan Edukasi Sains Vol. 6 No. 2 (2023): Jurnal Sains dan Edukasi Sains
Publisher : Faculty of Science and Mathematics, Universitas Kristen Satya Wacana, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/juses.v6i2p75-84

Abstract

Air yang menenuhi kriteria kualitas air yang bersih menjadi salah satu standar kualitas hidup masyarakat banyak. Akan tetapi seiring dengan berkembangnya zaman, ketersediaan air yang memenuhi kualitas sangat sulit ditemukan. Masyarakat Kecamatan Sukun yang mengakses air yang sudah terjamin bersih masih sangat minim. Penyakit diare menjadi penyakit berbasis lingkungan yang disebabkan oleh konsumsi air yang yang paling banyak terjadi di Kecamatan Sukun. Penelitian ini bertujuan untuk mengetahui keterkaitan antara kualitas air tanah dangkal dengan tingkat risiko terkena penyakit pada masyarakat di Kecamatan Sukun Kota Malang. Kualitas air tanah dianalisis dengan menggunakan metode Water Quality Index. Tingkat risiko terkena penyakit dianalisis dengan metode Quantitative Microbiological Risk Assessment. Sedangkan untuk mengetahui keterkaitannya menggunakan uji korelasi Pearson Product Moment. Hasil dari penelitian menunjukkan parameter E. coli tergolong cukup tinggi, sedangkan untuk parameter pH, TDS dan suhu tergolong dalam kategori aman. Nilai indeks kualitas air berada pada kategori cukup, baik, dan sangat baik. Tingkat risiko terkena penyakit diare pada masyarakat tergolong tinggi. Keterkaitan antara kualitas air tanah dengan tingkat risiko terkena penyakit di lokasi penelitian tergolong dalam hubungan kuat negatif. Tingkat risiko terkena penyakit diare pada masyarakat tergolong tinggi, akan tetapi fakta di lapangan menujukkan bahwa masyarakat rata-rata tidak mengalami gangguan kesehatan khususnya diare. Hal ini disebabkan karena masyarakat menerapkan perilaku hidup bersih dan sehat dengan baik.
Using a Participatory Learning and Action Approach to Improve Young Papuans and Moluccans’ Knowledge of Herbal Drinks to Increase Immunity During the COVID-19 Pandemic Rully Adi Nugroho; Sri Kasmiyati; Elizabeth Betty Elok Kristiani; Vincentia Irene Meitiniarti; Agna Sulis Krave
Engagement: Jurnal Pengabdian Kepada Masyarakat Vol 5 No 2 (2021): November 2021
Publisher : Asosiasi Dosen Pengembang Masyarajat (ADPEMAS) Forum Komunikasi Dosen Peneliti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/engagement.v5i2.788

Abstract

The introduction of coronavirus disease (COVID-19) into the human population represents a tremendous medical and economical crisis. The immune system plays a central role in protecting against this novel virus. This activity aimed to empower young generation using participatory learning and action approach to improve their knowledge about medicinal plants that grow in Indonesia and whose anti-inflammatory, antioxidant, immunomodulatory, and antiviral properties. Organoleptic test results showed that Kunyit Asam (consisted of turmeric and ginger) and Tealang (consisted of butterfly pea and lemongrass) were highly favored by panelists. Our results provide valuable information on the medicinal plants used as an immunity booster within the studied community.
Combination of polyherbal Phyllanthus reticulatus with Zingiber officinale and Cymbopogon citratus to optimize the antioxidant capacity Elizabeth Betty Elok Kristiani; Sri Kasmiyati; Yohanes Martono
Pharmaciana Vol 14, No 1 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i1.28002

Abstract

Currently, the public is interested in polyherbal-based foods and beverages as a source of natural antioxidants. The aim of the study is to evaluate the antioxidant properties and the phenolic and flavonoid compounds of formulations containing Z. officinale, C. citratus, and P. reticulatus (ZCP). Each sample was extracted using the maceration process in an ethanol solvent at room temperature for three 72-hour periods. There were fourteenth formulation of Z. officinale rhizome, C. citratus leaves, and P. reticulatus fruit which used Design of Expert (DoE). The DPPH method was used to determine the power of antioxidants. The flavonoid content of the extract was measured using the colorimetric method and AlCl3 reagent, while phenolics content using Folin-Ciocalteu. The formulations ZCP 1:0:0, 0:0:1, and 1:1:1 showed the antioxidant capacity in a strong categorization, with an IC50 value less than 50 µg/ml, while ZCP 0:1:0 was in a weak categorization (IC50 > 250 µg/ml). Another ZCP formulation was in a medium category. The ZCP 1:1:1 formulation was suggested as the best one for this investigation, which contains three plant samples. This formulation is interesting for further toxicity studies and in vivo testing so that it can be applied as an antioxidant-rich supplement product.
The determination of antioxidant and lead content of hemiparasite Dendrophthoe vitellina (F. Muell) Tiegh on Nutmeg Anna Helena Sabandar; Sri Kasmiyati; Djohan Djohan
Pharmaciana Vol 13, No 3 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i3.26780

Abstract

 Mistletoe, a parasitic plant growing on nutmeg trees, is conventionally perceived as a parasitic entity with limited regard for its potential medicinal value. On the other hand, mistletoe has a remarkable potential source of valuable medicinal compounds, particularly in traditional healthcare, due to its secondary metabolites such as flavonoid, phenolic, ascorbic acid, and antioxidant activity. Nevertheless, both the host tree and the environment, like Pb, influence the adaptive responses of secondary metabolites. This study aimed to determine the secondary metabolites, such as flavonoids, phenolics, ascorbic acid, antioxidant activity, and Pb content in the leaves of D. vitellina and nutmeg (M. fragrans). The samples were obtained via maceration using ethanol. The spectrophotometric analysis method was used to measure several parameters, using particular reagents for phenolic compounds using Folin-Ciocalteu, flavonoids using AlCl3, and ascorbic acid using sulfosalicylic acid), antioxidant activity using DPPH, and Pb using the AAS method. The results indicated that D. vitellina possesses a higher concentration of flavonoid and phenolic compounds, followed by 3.36 ± 1.92 % (w/w) and 18.45 ± 2.35 % (w/w) respectively. Conversely, nutmeg had a significant ascorbic acid concentration of 3.99 ± 4.38 % (w/w). The significant presence of phenolics and flavonoids had a crucial role in the antioxidant activity seen in D. vitellina, which exhibited exceptionally potent antioxidant properties. All samples contain Pb, ranging from 0.00001–0.00021 % (w/w), within the maximum allowable limits. Therefore, mistletoe is notable for being a reliable and encouraging plant species that can provide a natural supply of antioxidants and be safely used in traditional medicine.
Efektivitas Tumbuhan Mata Lele (Lemna minor L.) dengan Kombinasi Probiotik dalam Menurunkan Kadar Amonia dan Fosfat pada Air Kolam Budidaya Ikan Lele Fathiya Ulfa Nurrasyida; Sri Kasmiyati; Sucahyo Sucahyo
Jurnal Ilmu Lingkungan Vol 22, No 5 (2024): September 2024
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jil.22.5.1108-1113

Abstract

Kegiatan budidaya ikan lele dalam pemeliharaannya menghasilkan amonia dan fosfat yang tinggi. Kadar amonia dan fosfat yang berasal dari feses ikan dan sisa pakan mempengaruhi kualitas perairan dan kelangsungan hidup ikan lele. Tumbuhan air Lemna minor diketahui mampu memanfaatkan unsur hara terutama N dan P sebagai nutrient untuk  pertumbuhannya. Penelitian ini bertujuan untuk menentukan efektivitas Lemna minor dengan kombinasi probiotik sebagai agen bioremediasi dalam menurunkan amonia dan fosfat pada air kolam budidaya ikan lele. Parameter kualitas air yang diukur berupa amonia, fosfat, BOD, total padatan terlarut (TDS), oksigen terlarut (DO), suhu, dan pH. Penelitian dilakukan secara eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) dengan dua faktor. Data yang diperoleh dianalisis menggunakan two-way ANOVA dilanjutkan dengan uji Duncan Multirange Test (DMRT). Hasil penelitian menunjukkan tumbuhan Lemna minor mampu menurunkan kadar amonia, fosfat dan BOD pada media perlakuan dengan nilai efisiensi penurunan berkisar antara 70,06%-91,81% (amonia), 73,5%-92,6% (fosfat), 28,8%-42,8% (BOD). Kombinasi antara tumbuhan Lemna minor dan probiotik meningkatkan efektivitas dalam menurunkan amonia dan fosfat pada air kolam budidaya ikan lele.
Efek Interaktif Selenium dan Kromium Terhadap Pertumbuhan Vegetatif dan Generatif Tagetes erecta L. Tambunan, Roslinda Kristin; Kasmiyati, Sri
Buletin Anatomi dan Fisiologi Volume 8, Nomor 2, Tahun 2023
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.8.2.2023.96-105

Abstract

 Ffek toksik kromium (Cr) pada tanaman menyebabkan terjadinya perubahan pertumbuhan. Penggunaan unsur mikronutrien selenium merupakan salah satu strategi yang efektif untuk menurunkan efek toksik logam berat termasuk Cr dan mengatur pertumbuhan serta perkembangan tanaman. Tujuan penelitian ini menganalisis efek interaktif selenium dan kromium terhadap pertumbuhan vegetatif dan generatif Tagetes erecta.  Efek interaktif selenium dan kromium dikaji secara eksperimental menggunakan rancangan acak lengkap (RAL) dengan 2 faktor yaitu konsentrasi ion kromat (CrO42-) dalam bentuk K2CrO4 dengan 3 level konsentrasi (0, 50 dan 100 mg//L), dan konsentrasi selenium dalam bentuk selenat (SeO42-) dengan 3 level konsentrasi (0 μM/L, 40 μM/L, dan 80 μM/L). Interaksi selenium dan kromium mempengaruhi secara nyata pertumbuhan vegetatif dan generatif T. erecta. Penambahan selenium 80 μM/L mampu meningkatkan tinggi tanaman, jumlah daun, panjang akar dan jumlah bunga berturut-turut 8,5%, 11,5%, 4,5%, dan 6,0% dibandingkan tanaman kontrol (tanpa Se) pada tanaman dengan perlakuan Cr 50 mg/L. Pada penambahan Se sebesar 40 dan 80 μM/L, T. erecta dengan perlakuan Cr 100 mg/L terjadi penurunan tinggi tanaman dan panjang akar sebesar 5,4-5,9% dan 2,6-3,3% dibanding tanaman kontrol. Penambahan Se 40 dan 80 μM/L pada tanaman dengan perlakuan Cr 100 mg/L meningkatkan jumlah daun dan jumlah bunga secara nyata dibanding tanaman kontrol. Penelitian yang mengkaji tentang dosis dan peran Se dalam mekanisme toleransi terhadap logam Cr masih perlu dilakukan.   The toxic effects of chromium (Cr) on plants is to cause growth changes. The use of the micronutrient selenium is an effective strategy to reduce the toxic effects of heavy metals including Cr and regulate plant growth and development. The aim of this study was to analyze the interactive effects of selenium and chromium on the vegetative and generative growth of Tagetes erecta. The interactive effect of selenium and chromium was studied experimentally using a completely randomized design (CRD) with 2 factors, namely the concentration of chromate ion (CrO42-) in the form of K2CrO4 with 3 concentration levels (0, 50 and 100 mg//L), and the concentration of selenium in the form of selenat (SeO42-) with 3 concentration levels (0 μM/L, 40 μM/L, and 80 μM/L). The interaction of selenium and chromium significantly affects the vegetative and generative growth of T. erecta. The addition of 80 µM/L selenium was able to increase plant height, number of leaves, root length and number of flowers 8.5%, 11.5%, 4.5% and 6.0% compared to control plants (without Se), on plants treated with Cr 50 mg/L. The addition of Se of 40 and 80 μM/L, T. erecta with Cr 100 mg/L treatment reduced plant height and root length by 5.4-5.9% and 2.6-3.3% compared to control plants. In contrast, the addition of Se 40 and 80 μM/L to plants treated with Cr 100 mg/L significantly increased the number of leaves and the number of flowers compared to the control plants. Research that examines the dose and role of Se in the mechanism of tolerance to Cr metal still needs to be done.
Co-Authors ,, Djohan Agna Sulis Krave Agustien Sri Noerwahju Alberthnico, Ferdian Andreas Binar Aji Sukmana Anggara Mahardika Anna Helena Sabandar Araujo, Otilda Fatima Ariyanto, Prasetyo Eko Betty Elok, Elizabeth Cahyaningrum, Desti Christian Cantika Glodia Debora Kristiani Rahardjo Desti Christian Cahyaningrum Dewi Prabawaningrum Djohan Djohan Djohan Djohan Djurubasa, Dani Karel Marthin Egarani, Grahita Rezhi Elizabeth B.E Kristiani Elizabeth Betty Elok Kristiani Elizabeth Bety E.K Fanny Nurwahidah, Cantika Fathiya Ulfa Nurrasyida Fatima Araujo, Otilda Gabriella L. Mataradja Hapsari Sekar Widiantari, Olive Ie, Johanes Juan Yutama Putra Indrianasari, Putri Irfan Dwidja Priyambada, Irfan Dwidja Jokosulistyo Wartanto Juliana Sarioa Kachingwe, Baxter Hepburn Kapitarauw, Agnesya Giovani Putri Cendana Keliat, Natalia Rosa Kumala Dewi L. Hartanto Nugroho Lani, Rischa Karmila Margareta Prameswati Maria Marina Herawati Monica Mangudis, Vicharisti Nabila, Nabila Natalia Rosa Keliat Novellasari, Firdiana Penina Intansari Prasgi, Henokh Christian Pratama, Dimas Seno Bagus Priyayi, Desi Fajar Purwoko, Agus Putra Ie, Johanes Juan Yutama Putri, A C. Rahardja, P M. Ramagita, Marsahanda Astri Rimanti, Audri Septina Putri Rintawati Sandradewi, Rintawati Risya Pramana Situmorang Rully Adi Nugroho Sabandar, Anna Helena Santosa S, Santosa Satria Dharmawan Kustia Dewa Seleiman, Mahmoud F. Setya Magdalena, Ajeng Shinta, Damaris Clara Siahaya, Esty Vicariat Sucahyo Sucahyo Sucahyo Susanti Pudji Hastuti Susanti Puji Hastuti Syuhuud Arumbinang Wajdi Tambunan, Roslinda Kristin Trisianna, Jessica Ayu Trisianna Vincentia Irene Meitiniarti Vini Kurnia Ramadhani Wahyu Hari Kristiyanto Widyaningrum, Indy Yohanes Martono Yolanda Halim, Vivian