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Eksplorasi Keterampilan Kolaboratif Peserta didik pada Materi Fotosintesis dan Pembelahan Mitosis Keliat, Natalia Rosa; Situmorang, Risya Pramana; Cahyaningrum, Desti Christian; Kasmiyati, Sri; Betty Elok, Elizabeth; Binar Aji Sukmana, Andreas; Yolanda Halim, Vivian; Setya Magdalena, Ajeng; Monica Mangudis, Vicharisti; Hapsari Sekar Widiantari, Olive; Fanny Nurwahidah, Cantika; Fatima Araujo, Otilda
Al-Khidmah Jurnal Pengabdian Masyarakat Vol. 5 No. 2 (2025): MEI-AGUSTUS
Publisher : Institute for Research and Community Service (LPPM) of the Islamic University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56013/jak.v5i2.4404

Abstract

This activity aims to explore and improve the collaborative skills of students at SMA Kristen 1, Salatiga with class XII IPA students as participants. The data collection technique used a collaborative skills observation sheet with indicators 1) working productively; 2) being responsible; 3) respecting other students and; 4) compromising. The data analysis technique used the percentage of collaborative skills. In photosynthesis learning, students with collaborative skills in the exemplary category reached 14 people (19.4%), proficient 47 people (65.3%), basic 9 people (12.5%), and novice only 2 people (2.8%). In mitosis learning, students who had collaborative skills in the exemplary category were only 9 people (12.5%), proficient 52 people (72.2%), basic 8 people (11.1%), and novice only 3 people (4.2%). In the aspect of working productively in photosynthesis learning, the novice category reached 8 people (11.1%), while in mitosis learning the novice category was only 2 people (2.8%). In the aspect of being responsible for photosynthesis, the novice category reached 9 people (12.5%), while in mitosis learning, the novice category was only 4 people (5.6%). Furthermore, in photosynthesis learning in the aspect of flexibility and compromise, the novice category reached 8 people (11.1%), while in mitosis learning the novice category was only 7 people (9.7%). Based on these findings, it can be concluded that there has been an increase in the number of students who are increasingly skilled in collaborating with students.
The determination of antioxidant and lead content of hemiparasite Dendrophthoe vitellina (F. Muell) Tiegh on Nutmeg Sabandar, Anna Helena; Kasmiyati, Sri; Djohan, Djohan
Pharmaciana Vol. 13 No. 3 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i3.26780

Abstract

 Mistletoe, a parasitic plant growing on nutmeg trees, is conventionally perceived as a parasitic entity with limited regard for its potential medicinal value. On the other hand, mistletoe has a remarkable potential source of valuable medicinal compounds, particularly in traditional healthcare, due to its secondary metabolites such as flavonoid, phenolic, ascorbic acid, and antioxidant activity. Nevertheless, both the host tree and the environment, like Pb, influence the adaptive responses of secondary metabolites. This study aimed to determine the secondary metabolites, such as flavonoids, phenolics, ascorbic acid, antioxidant activity, and Pb content in the leaves of D. vitellina and nutmeg (M. fragrans). The samples were obtained via maceration using ethanol. The spectrophotometric analysis method was used to measure several parameters, using particular reagents for phenolic compounds using Folin-Ciocalteu, flavonoids using AlCl3, and ascorbic acid using sulfosalicylic acid), antioxidant activity using DPPH, and Pb using the AAS method. The results indicated that D. vitellina possesses a higher concentration of flavonoid and phenolic compounds, followed by 3.36 ± 1.92 % (w/w) and 18.45 ± 2.35 % (w/w) respectively. Conversely, nutmeg had a significant ascorbic acid concentration of 3.99 ± 4.38 % (w/w). The significant presence of phenolics and flavonoids had a crucial role in the antioxidant activity seen in D. vitellina, which exhibited exceptionally potent antioxidant properties. All samples contain Pb, ranging from 0.00001–0.00021 % (w/w), within the maximum allowable limits. Therefore, mistletoe is notable for being a reliable and encouraging plant species that can provide a natural supply of antioxidants and be safely used in traditional medicine.
Combination of polyherbal Phyllanthus reticulatus with Zingiber officinale and Cymbopogon citratus to optimize the antioxidant capacity Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Martono, Yohanes
Pharmaciana Vol. 14 No. 1 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i1.28002

Abstract

Currently, the public is interested in polyherbal-based foods and beverages as a source of natural antioxidants. The aim of the study is to evaluate the antioxidant properties and the phenolic and flavonoid compounds of formulations containing Z. officinale, C. citratus, and P. reticulatus (ZCP). Each sample was extracted using the maceration process in an ethanol solvent at room temperature for three 72-hour periods. There were fourteenth formulation of Z. officinale rhizome, C. citratus leaves, and P. reticulatus fruit which used Design of Expert (DoE). The DPPH method was used to determine the power of antioxidants. The flavonoid content of the extract was measured using the colorimetric method and AlCl3 reagent, while phenolics content using Folin-Ciocalteu. The formulations ZCP 1:0:0, 0:0:1, and 1:1:1 showed the antioxidant capacity in a strong categorization, with an IC50 value less than 50 µg/ml, while ZCP 0:1:0 was in a weak categorization (IC50 > 250 µg/ml). Another ZCP formulation was in a medium category. The ZCP 1:1:1 formulation was suggested as the best one for this investigation, which contains three plant samples. This formulation is interesting for further toxicity studies and in vivo testing so that it can be applied as an antioxidant-rich supplement product.
The Phytochemical and Biological Activities of Two Phyllanthus Species: Insights into Metabolit, Antioxidant and Antibacterial Activity Araújo, Otilda Fátima; Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Nugroho, Laurentius Hartanto
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.13944

Abstract

Phylanthus species, including P. niruri and P. urinaria have pharmacological potential due to their rich phytochemical composition. People usually used this plant for medicinal treatments. This study aimed to compare the phytochemical and antioxidant and antibacterial activities of two Phyllanthus species. The whole of plants was extracted using maceration method with ethanol as solvent. Phytochemical content analyzed using spectrophotometer.  The reagent used for each compounds that were Folin-ciocalteu for phenolics, AlCl3 for flavonoid, dimethyl sulfoxide for chlorophyll and carotenoid. Bioactivity analysis using 1,1-diphenyl-2-picrylhydrazyl method for antioxidant activity and Kirby-Bauer method for antibacterial activity. The highest flavonoid content (12.22 mg QE/gram extract) and total chlorophyll (43.2 µg/ml extract) in P. niruri while phenolic content (80.8 mg GAE/gram extract) in P. urinaria. The carotene of both Phyllanthus were similar (11.9 µg/ml extract. The IC50 values of P. urinaria (6.16 ± 0.42 μg/ml) and P. niruri (17.72 ± 0.80 μg/ml), which indicated very strong antioxidant activity. P. urinaria leaf extract had stronger inhibition against Escherichia coli than Staphylococcus aureus (>20 mm) and P. niruri leaf extract could inhibit E. coli and S. aureus bacteria at all concentrations (11-20 mm). This study found that phenolic compounds strongly influenced the antioxidant and antibacterial abilities of Phyllanthus, while chlorophyll and carotenoids had only a slight influence. These findings open up opportunities to utilize P. niruri and P. urinaria as antioxidant and antibacterial agents.
Extract Formulation of Zingiber officinale and Phylanthus reticulatus Induces Apoptosis of WiDr Colon Cancer Cells Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Martono, Yohanes; Kachingwe, Baxter Hepburn
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.25776

Abstract

People use ginger (Zingiber officinale) extensively for health purposes. Our previous studies have shown that Phyllanthus reticulatus has strong antioxidant capabilities. This study investigates the anticancer potential of ethanolic extract of Z. officinale and P. reticulatus formulations (ZP), focusing on their cytotoxicity and mechanisms against cancer cells. Samples were extracted using the maceration method. The method for determining the cytotoxicity of cells is MTT, the cell cycle is flow cytometry, apoptotic cells are flow cytometry and double staining, and caspase-8 and caspase-9 expression using the immunocytochemistry method. The analysis of caspase-8 and caspase-9 expression using the immunocytochemistry method. Among the tested formulations, the ethanolic extract of ZP_1:0 showed the most promise against WiDr colon cancer cells (IC50 value of 102.1 µg/ml). The ZP_1:0 demonstrated dose-dependent apoptosis induction in WiDr cells, showing early and late apoptosis, with minimal necrosis. The extract activates extrinsic and intrinsic apoptotic pathways in inducing cancer cell death. Compared to doxorubicin, the ZP 1:0 formulation selectively promotes apoptosis with reduced necrotic damage, suggesting the potential for reduced systemic toxicity. The study supports the potential of Z. officinale as a source of safer, plant-based anticancer agents, particularly for colon cancer, while recommending further research to identify and enhance active compounds.
Dualisme Pemanfaatan Gulma Genjer (Limnocharis flava): Peluang Sebagai Agen Fitoremediasi dan Pangan Nutraseutika Ramagita, Marsahanda Astri; Ie, Johanes Juan Yutama Putra; Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.10270

Abstract

Tanaman Genjer (Limnocharis flava) adalah salah satu jenis gulma yang memiliki fungsi ganda sebagai bahan pangan nutraseutikan dan agen fitoremediasi. Pemanfaatan Genjer sebagai bahan pangan didasarkan pada kandungan metabolit sekunder yang terdapat didalamnya, seperti flavonoid, fenol, serta antioksidan yang berpotensi untuk dikembangkan sebagai bahan pangan fungsional serta obat tradisional. Selain itu, kemampuan tanaman Genjer sebagai agen fitoremediasi dalam menyerap senyawa pencemar seeperti logam berat, menjadikannya efektif dalam pengelolaan limbah cair. Tujuan review artikel ini dibuat untuk mengeksplorasi keunggulan dan tantangan dalam pengaplikasiannya. Metode penelitian ini yaitu literature review dengan menentukan topik yang dikaji, mengumpulkan artikel – artike, evaluasi, dan interpretasi hasil. Artikel yang digunakan merupakan artikel penelitian dari tahun 2015-2024 dan berasal dari google scholar. Hasil review menunjukkan bahwa tanaman Genjer tidak hanya berperan dalam kesehatan manusia melalui kandungan nutrisinya. Tapi juga mendukung keberlanjutan lingkungan dengan kemampuan sebagai agen fitoremediasi. Dengan pemahaman yang lebih lanjut, tanaman Genjer dapat dioptimalkan sebagai solusi yang inovatif dalam bidang pangan serta lingkungan.
THE APPLICATION OF Fe AND Cr(III) IN GROWING MEDIA AND ITS EFFECT ON PLANT GROWTH AND Cr(III) OXIDATION ON Tagetes erecta Kasmiyati, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1784

Abstract

The oxidation of Cr(III) to Cr(VI) in the environment has a detrimental impact because it can change the form of non-toxic Cr(III) to Cr(VI), which is toxic to organisms. The study aimed to examine the effect of the application of iron (Fe) and trivalent chromium (Cr(III)) compounds in Tagetes erecta growing media on growth and Cr(III) oxidation. Concentrations of Cr(III) 0, 100, and 500 mg/L and Fe 0, 3, 15, and 30 mg/L were applied to the growing media of T. erecta as the model plant. The growth and accumulation of Cr(VI) in plants were measured to determine the effect of Fe and Cr(III) treatment on growth and Cr(III) oxidation. The accumulation of Cr(VI) in the roots and shoots of T. erecta increased significantly due to the addition of Fe in the growing media treated with Cr(III). The highest accumulation of Cr(VI) in the roots and shoots of T. erecta found in the treatment of Cr(III) 500 mg/L and Fe 30 mg/L, were respectively 0.092 g/L and 0.070 g/L. The addition of Fe in growing media containing Cr(III) increased plant height, root length, and shoot dry weight but decreased leaf number and root dry weight. T. erecta root biomass was more affected by the toxic impact of Fe than Cr(III). On the other hand, the inhibition of leaf formation was caused by the toxic effect of Cr(III) rather than Fe.
Perbandingan Kekuatan Antibakteri Minyak Atsiri dari Tiga Varietas Jahe (Zingiber officinale Rosc) Terhadap Baccillus subtilis dan Pseudomonas aeruginosa Kristiani, Elizabeth Betty Elok; Kaya, Emma Sharon Admatha; Kasmiyati, Sri
Buletin Anatomi dan Fisiologi Volume 11, Nomor 1, Tahun 2026
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.1.1.2026.9-15

Abstract

Jahe (Zingiber officinale) merupakan tanaman rempah yang banyak tumbuh di Indonesia dan diketahui memiliki potensi sebagai agen antibakteri. Penelitian ini bertujuan untuk membandingkan aktivitas antibakteri minyak atsiri dari tiga varietas jahe yaitu jahe gajah (Z. officinale var. Roscoe), jahe merah (Z. officinale var Rubrum), dan jahe emprit (Z. officinale var Amarum)  terhadap bakteri Bacillus subtilis dan Pseudomonas aeruginosa. Ekstraksi minyak atsiri menggunakan teknik sohkletasi dengan pelarut etanol. Uji aktivitas antibakteri menggunakan metode difusi cakram. Rendemen minyak atsiri tertinggi Z. officinale var Rubrum 23,45 ± 3,32 %, diikuti Z. officinale var Amarum 19,90 ± 2,17 % dan terendah Z. officinale var. Roscoe 15,70 ± 2,42 %. Pada semua konsentrasi uji, kemampuan minyak atsiri jahe dalam menghambat pertumbuhan Baccillus subtilis lebih kecil secara signifikan dibandingkan tetrasiklin dalam tetapi lebih besar pada P. aeruginosa. Hasil uji antibakteri minyak atsiri semua tiga varietas jahe pada konsentrasi 62,6 – 1000 µg/mL terbukti menghambat pertumbuhan kedua bakteru uji dalam kategori kuat yaitu diamter daya hambat > 20 mm. Kekuatan aktivitas antibakteri minyak atsiti terhadap B. Subtilis lebih rendah dari antibiotika standar tetrasiklin, sedangkan terhadap P. aeruginosa lebih kuat. Minyak atsiri ketiga variets berpotensi untuk dijadikan sebagai agen antibakteri terhadap strain gram negatif dan gram positif.  Ginger (Zingiber officinale) is a spice plant that grows widely in Indonesia and is known to have potential as an antibacterial agent. This study aims to compare the antibacterial activity of essential oils from three varieties of ginger, namely elephant ginger (Z. officinale var. Roscoe), red ginger (Z. officinale var Rubrum), and emprit ginger (Z. officinale var Amarum) against Bacillus subtilis and Pseudomonas aeruginosa bacteria. Essential oil extraction was performed using the Soxhlet extraction technique with ethanol as the solvent. Antibacterial activity was tested using the disk diffusion method. The highest essential oil yield was obtained from Z. officinale var. Rubrum at 23.45 ± 3.32%, followed by Z. officinale var. Amarum at 19.90 ± 2.17% and the lowest from Z. officinale var. Roscoe at 15.70 ± 2.42%. At all test concentrations, the ability of ginger essential oil to inhibit the growth of Bacillus subtilis was significantly lower than that of tetracycline, but higher than that of P. aeruginosa. The antibacterial test results of essential oils from all three ginger varieties at concentrations of 62.6–1000 µg/mL proved to inhibit the growth of both test bacteria in the strong category, namely with an inhibition diameter > 20 mm. The strength of the antibacterial activity of essential oils against B. subtilis was lower than the standard antibiotic tetracycline, while against P. aeruginosa it was stronger. The essential oils of the three varieties have the potential to be used as antibacterial agents against gram-negative and gram-positive strains.
EXPLORATION OF THE POTENTIAL OF ASTERACEAE FAMILI PLANTS ETHANOL EXTRACT AS BIOLARVICIDES AGAINST THIRD-INSTAR Aedes aegypti LARVAE Dwianto, Stefanus Agung; Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok
Berita Biologi Vol 25 No 1 (2026): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2026.14933

Abstract

The improper use of synthetic larvicides results in detrimental environmental impacts. To mitigate these effects, biolarvicides serve as a viable alternative. Plants within the Asteraceae family contain bioactive compounds with potential biolarvicidal properties. This research aimed to explore the potential of Asteraceae species as biolarvicides. This research utilizes Artemisia vulgaris, Cosmos caudatus, Eclipta prostrata, and Tagetes erecta. Extraction was conducted by the maceration method using 96% ethanol. The resulting extracts were analyzed for secondary metabolite content. Data were analyzed using SAS 9.0 software Two-way ANOVA, followed by Tukey’s post-hoc test. Larvicidal bioassays were performed on third-instar Aedes aegypti larvae at concentrations of 1,000-10,000 ppm. The parameters measured included mortality percentage, LC50, and LC90. Morphological changes in dead larvae were observed and compared against a control group. The results indicated that secondary metabolite in the leaf ethanolic extracts were higher than those in the stems and roots. The leaf ethanolic extract of C. caudatus exhibited the highest content of flavonoids (928.3±368.7 mg/g), phenolics (310.2±84.9 mg/g), tannins (187.1± 55.5 mg/g), and saponins (314±95.4 mg/g extract). Conversely, the highest alkaloid content was recorded in the leaf extract of T. erecta (27.8±7.1 mg/g). The highest mortality rate was observed in larvae exposed to A. vulgaris leaf extract, reaching 100% mortality across all concentrations within 24 hours. Consequently, LC50 and LC90 values for A. vulgaris could not be determined. For C. caudatus, the LC50 values were 7204.7 ppm (24 h) and 6839.7 ppm (48 h), while LC90 values were 9077.3 ppm (24 h) 8226.4 ppm (48 h), respectively. E. prostrata yielded an LC50 of 10255.6 ppm and an LC90 of 18048.9 ppm at 24 hours; however, these values were not detectable at 48 hours. T. erecta showed an LC50 of 6569.6 ppm (24 h) and 6397.1 ppm (48 h), with LC50 values of 9682.1 ppm and 9343.3 ppm, respectively. Morphological observations of dead larvae exposed the extracts alterations, including pigmentation, elongation, and shrinkage. While the leaf ethanolic extract of C. caudatus contained the highest concentration of secondary metabolites. The leaf ethanolic extract of A. vulgaris demonstrated the greatest potential as a biolarvicide.
Peningkatan Kapasitas Penelitian Guru dan Siswa SMA Melalui Pelatihan Metodologi Penelitian dan Pendampingan Olimpiade Penelitian Siswa Indonesia Sukmana, Andreas A.; Kasmiyati, Sri; Kristiani, Betty E.; Purnomo, Hindriyanto D.; Kristianto, Budhi; Krismiyati, Krismiyati; Bayu, Teguh I.; Tanone, Radius; Nugroho, Adi; Yulia, Hanita; Mailoa, Evangs; Christianto, Erwien
JURNAL PENGABDIAN PAPUA Vol 10 No 1 (2026)
Publisher : LPPM Uncen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31957/jpp.v10i1.5252

Abstract

Critical and innovative thinking skills are essential requirements for students to meet the global challenges. One strategy to develop these skills is through participation in the Indonesian Student Research Olympiad (OPSI), which requires collaboration between teachers and students in implementing fundamental research concepts. However, the implementation of basic research concepts within the high school curriculum remains limited. Consequently, collaboration with higher education institutions serves as a potential solution, specifically through research training and mentoring. This training initiative aimed to enhance the research competence of teachers and students at SMAN 1 Ambarawa by providing methodology training and mentoring for OPSI research teams, conducted by a faculty team from Satya Wacana Christian University. The program was conducted intensively consisting of research design training and research mentoring for nine student groups. The training and the mentoring were provided by lecturers whose expertise aligned with the specific OPSI proposal topics of each group. This mentoring resulted in an overall improvement in the research capabilities of both teachers and students, with one group successfully won a gold medal at OPSI 2025. In conclusion, this program results in a positive reception, which expressed the hope that similar initiatives can be conducted on a regular basis.
Co-Authors ,, Djohan Adi Nugroho Agna Sulis Krave Agustien Sri Noerwahju Alberthnico, Ferdian Andreas Binar Aji Sukmana Anggara Mahardika Anna Helena Sabandar Araujo, Otilda Fatima Ariyanto, Prasetyo Eko Bayu, Teguh I. Berutu, Cocok A.M. Betty Elok, Elizabeth Bety E.K, Elizabeth Budhi Kristianto Cahyaningrum, Desti C. Cahyaningrum, Desti Christian Cantika Glodia Debora Kristiani Rahardjo Desti Christian Cahyaningrum Dewi Prabawaningrum Djohan Djohan Djohan Djohan Djurubasa, Dani Karel Marthin Dwianto, Stefanus Agung Egarani, Grahita Rezhi Elizabeth B.E Kristiani Elizabeth Betty Elok Kristiani Erwien Christianto Evangs Mailoa Fanny Nurwahidah, Cantika Fathiya Ulfa Nurrasyida Fatima Araujo, Otilda Gabriella L. Mataradja Hanita Yulia Hapsari Sekar Widiantari, Olive Hastuti, Susanti P. Ie, Johanes Juan Yutama Putra Indrianasari, Putri Irfan Dwidja Priyambada, Irfan Dwidja Juliana Sarioa Kachingwe, Baxter Hepburn Kapitarauw, Agnesya Giovani Putri Cendana Kaya, Emma Sharon Admatha Keliat, Natalia R. Keliat, Natalia Rosa Krismiyati Kristiani, Betty E. Kristiani, Elizabeth B.E. Kumala Dewi L. Hartanto Nugroho Lani, Rischa Karmila Margareta Prameswati Maria Marina Herawati Meitiniarti, V. I. Monica Mangudis, Vicharisti Nabila, Nabila Natalia Rosa Keliat Novellasari, Firdiana Nugroho, Rully A. Penina Intansari Poluan, Risky H. Prasgi, Henokh Christian Pratama, Dimas Seno Bagus Priyayi, Desi Fajar Purnomo, Hindriyanto D. Purwoko, Agus Putra Ie, Johanes Juan Yutama Putri, A C. Radius Tanone Rahardja, P M. Ramagita, Marsahanda Astri Rimanti, Audri Septina Putri Rintawati Sandradewi, Rintawati Risya Pramana Situmorang Rully Adi Nugroho Sabandar, Anna Helena Santosa S, Santosa Satria Dharmawan Kustia Dewa Seleiman, Mahmoud F. Setya Magdalena, Ajeng Shinta, Damaris Clara Siahaya, Esty Vicariat Situmorang, Risya P. Sucahyo Sucahyo Sucahyo Sukmana, Andreas A. Sukmana, Andreas B.A. Susanti Pudji Hastuti Susanti Puji Hastuti Syuhuud Arumbinang Wajdi Tambunan, Roslinda Kristin Trisianna, Jessica Ayu Trisianna Vincentia Irene Meitiniarti Vini Kurnia Ramadhani Wahyu Hari Kristiyanto Wartanto, Jokosulistyo Widyaningrum, Indy Yohanes Martono Yolanda Halim, Vivian