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All Journal METAMORFOSA Journal of Biological Sciences Jurnal MIPA Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Pendidikan Biologi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioedukatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Bioedukasi: Jurnal Pendidikan Biologi Jurnal Sains dan Edukasi Sains Indigenous Biologi Proceeding Biology Education Conference Bioed : Jurnal Pendidikan Biologi BIOCHEPHY: Journal of Science Education Al-Khidmah Jurnal Pengabdian Masyarakat AGRIC Engagement: Jurnal Pengabdian Kepada Masyarakat Biosaintifika: Journal of Biology & Biology Education Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Pengabdian Kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia

Sri Kasmiyati

Universitas Kristen Satya Wacana

Author-ID : 516996

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Dualisme Pemanfaatan Gulma Genjer (Limnocharis flava): Peluang Sebagai Agen Fitoremediasi dan Pangan Nutraseutika Ramagita, Marsahanda Astri; Ie, Johanes Juan Yutama Putra; Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.10270

Tanaman Genjer (Limnocharis flava) adalah salah satu jenis gulma yang memiliki fungsi ganda sebagai bahan pangan nutraseutikan dan agen fitoremediasi. Pemanfaatan Genjer sebagai bahan pangan didasarkan pada kandungan metabolit sekunder yang terdapat didalamnya, seperti flavonoid, fenol, serta antioksidan yang berpotensi untuk dikembangkan sebagai bahan pangan fungsional serta obat tradisional. Selain itu, kemampuan tanaman Genjer sebagai agen fitoremediasi dalam menyerap senyawa pencemar seeperti logam berat, menjadikannya efektif dalam pengelolaan limbah cair. Tujuan review artikel ini dibuat untuk mengeksplorasi keunggulan dan tantangan dalam pengaplikasiannya. Metode penelitian ini yaitu literature review dengan menentukan topik yang dikaji, mengumpulkan artikel – artike, evaluasi, dan interpretasi hasil. Artikel yang digunakan merupakan artikel penelitian dari tahun 2015-2024 dan berasal dari google scholar. Hasil review menunjukkan bahwa tanaman Genjer tidak hanya berperan dalam kesehatan manusia melalui kandungan nutrisinya. Tapi juga mendukung keberlanjutan lingkungan dengan kemampuan sebagai agen fitoremediasi. Dengan pemahaman yang lebih lanjut, tanaman Genjer dapat dioptimalkan sebagai solusi yang inovatif dalam bidang pangan serta lingkungan.

Perkecambahan dan Pertumbuhan Kecambah Tagetes erecta pada Cekaman Kadmium Ariyanto, Prasetyo Eko; Kasmiyati, Sri; Sucahyo, Sucahyo
Buletin Anatomi dan Fisiologi Volume 10, Nomor 1, Tahun 2025
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.10.1.2025.63-70

Kadmium merupakan salah satu logam berat yang bersifat toksik dan dapat diserap oleh tumbuhan. Tagetes erecta merupakan tanaman hias yang memiliki kemampuan toleransi yang tinggi terhadap cekaman logam berat, sehingga tanaman ini dapat digunakan sebagai agen fitoremediasi. Tujuan dari penelitian ini adalah untuk mengetahui efek dari cekaman logam berat pada proses perkecambahan dan pertumbuhan benih T. erecta pada media pupuk cair yang mangandung Cd. Penelitian dilakukan secara eksperimental menggunakan desain rancangan acak lengkap. Konsentrasi CdCl2 yang digunakan sebagai perlakuan antara lain 0 (kontrol), 25, 50, 100, dan 200ppm. Biji T. erecta dikecambahkan di dalam cawan petri yang dialasi dengan kertas merang dan ditutup dengan plastik berwarna gelap. Perkecambahan dilakukan di laboratorium. Parameter jumlah biji yang berkecambah diamati setiap hari selama 14 hari digunakan sebagai penentuan persentase perkecambahan dan indeks vigor kecambah. Pertumbuhan kecambah diamati pada parameter panjang radikula dan panjang hipokotil pada akhir penelitian. Pemberian perlakuan kadmium mempengaruhi secara nyata perkecambahan dan pertumbuhan kecambah T. erecta. Kadmium pada konsentrasi 100-200 ppm menghambat secara nyata perkecambahan dan pertumbuhan kecambah T. erecta. Pertumbuhan radikula T. erecta pada perlakuan Cd sebesar 25ppm menunjukkan ukuran yang lebih panjang dibanding perlakuan kontrol. Hal ini dikarenakan penambahan kadmium pada konsentrasi rendah sebesar 25ppm masih mampu ditoleransi dan merangsang pertumbuhan kecambah T. erecta. Cadmium is one of the heavy metals that is toxic and can be absorbed by plants. Tagetes erecta is an ornamental plant that has a high tolerance to heavy metal stress, so this plant can be used as a phytoremediation agent. The purpose of this study was to determine the effects of heavy metal stress on the process of germination and growth of T. erecta seeds in liquid fertilizer media containing Cd. The research was conducted experimentally using a completely randomized design. The concentration of CdCl2 used as treatment included 0 (control), 25, 50, 100, and 200ppm. T. erecta seeds were germinated in a petri dish covered with straw paper and covered with dark colored plastic. Germination was carried out in the laboratory. The number of germinated seeds observed daily for 14 days was used to determine the germination percentage and vigor index of the sprouts. Sprout growth was observed in the parameters of radicle length and hypocotyl length at the end of the study. Cadmium treatment significantly affected the germination and growth of T. erecta sprouts. Cadmium at concentrations of 100-200 ppm significantly inhibited the germination and growth of T. erecta sprouts. Radicle growth of T. erecta in the Cd treatment of 25 ppm showed a longer size than the control treatment. This is because the addition of cadmium at a low concentration of 25 ppm can still be tolerated and stimulate the growth of T. erecta sprouts.

Peran Teknologi Nanopartikel Pada Budidaya, Produksi, dan Pemanfaatan Senyawa Tanaman Vanili (Vanilla planifolia) Rimanti, Audri Septina Putri; Djurubasa, Dani Karel Marthin; Kasmiyati, Sri; Kristiani, Elizabeth Betty Elok
Buletin Anatomi dan Fisiologi Volume 10, Nomor 2, Tahun 2025
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.10.2.2025.102-111

Aplikasi nanoteknologi, di dunia pertanian maupun kesehatan, kini semakin marak untuk dikembangkan. Vanilla planifolia atau tanaman vanili, yang merupakan penghasil senyawa vanilin, memiliki potensi pengaplikasian nanoteknologi. Peningkatan pertumbuhan tanaman dengan menggunakan teknologi nanofertilizer dan peningkatan senyawa metabolit sekunder vanilin dengan menggunakan teknologi nanoelisitor dapat memberikan hasil maksimal bagi panen tanaman vanili. Disisi lain, vanilin yang merupakan senyawa aktif pada tanaman vanili juga memiliki khasiat sebagai obat dan bahkan dapat dimanfaatkan dalam bidang kecantikan. Penelitian ini bertujuan untuk menggali peran nanoteknologi yang memiliki potensi pengaplikasiannya pada tanaman vanili, baik pada budidaya tanaman maupun pada pemanfaatan senyawa vanilin dalam bidang kesehatan dan kecantikan. Dari hasil yang didapatkan, nanoteknologi dalam bentuk nanofertilizer memiliki potensi besar dalam meningkatkan pertumbuhan tanaman vanili serta dapat membantu tanaman dalam menghadapi cekaman biotik dan abiotik yang mengganggu pertumbuhannya. Nanoteknologi juga dapat diaplikasikan pada senyawa vanilin, dimana vanilin dalam bentuk nanopartikel berperan sebagai anti-inflamasi, anti-kanker, anti-oksidan, serta berperan sebagai senyawa pengikat alami yang mendukung penyembuhan luka. Kemampuan tersebut dapat dimanfaatkan sebagai nanoteknologi dalam bidang kesehatan serta bidang kecantikan Applications of nanotechnology, in the agriculture and health sector, are now increasingly being developed. Vanilla planifolia or vanilla plants, which are producers of vanillin compounds, have the potential for nanotechnology applications. Increasing plant growth using nanofertilizer technology and increasing vanillin secondary metabolite compounds using nanoelicitor technology can provide maximum yield for vanilla plant. On the other hand, vanillin, which is the active compound in the vanilla plant, also has medicinal properties and can even be used in the field of beauty. This research aims to explore the role of nanotechnology that has the potential for application in vanilla plants, both in plant cultivation and in the use of vanillin compounds in the field of health and beauty. From the results obtained, nanotechnology in the form of nanofertilizer has great potential in increasing the growth of vanilla plants and can help plants in dealing with biotic and abiotic stress that interferes with their growth. Nanotechnology can also be applied to vanillin compounds, where vanillin in the form of nanoparticles acts as an anti-inflammatory, anti-cancer, anti-oxidant, and also acts as a natural binding compound that supports wound healing. This ability can be used as nanotechnology in the health and beauty fields

Eksplorasi Keterampilan Kolaboratif Peserta didik pada Materi Fotosintesis dan Pembelahan Mitosis Keliat, Natalia Rosa; Situmorang, Risya Pramana; Cahyaningrum, Desti Christian; Kasmiyati, Sri; Betty Elok, Elizabeth; Binar Aji Sukmana, Andreas; Yolanda Halim, Vivian; Setya Magdalena, Ajeng; Monica Mangudis, Vicharisti; Hapsari Sekar Widiantari, Olive; Fanny Nurwahidah, Cantika; Fatima Araujo, Otilda
Al-Khidmah Jurnal Pengabdian Masyarakat Vol. 5 No. 2 (2025): MEI-AGUSTUS
Publisher : Institute for Research and Community Service (LPPM) of the Islamic University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56013/jak.v5i2.4404

This activity aims to explore and improve the collaborative skills of students at SMA Kristen 1, Salatiga with class XII IPA students as participants. The data collection technique used a collaborative skills observation sheet with indicators 1) working productively; 2) being responsible; 3) respecting other students and; 4) compromising. The data analysis technique used the percentage of collaborative skills. In photosynthesis learning, students with collaborative skills in the exemplary category reached 14 people (19.4%), proficient 47 people (65.3%), basic 9 people (12.5%), and novice only 2 people (2.8%). In mitosis learning, students who had collaborative skills in the exemplary category were only 9 people (12.5%), proficient 52 people (72.2%), basic 8 people (11.1%), and novice only 3 people (4.2%). In the aspect of working productively in photosynthesis learning, the novice category reached 8 people (11.1%), while in mitosis learning the novice category was only 2 people (2.8%). In the aspect of being responsible for photosynthesis, the novice category reached 9 people (12.5%), while in mitosis learning, the novice category was only 4 people (5.6%). Furthermore, in photosynthesis learning in the aspect of flexibility and compromise, the novice category reached 8 people (11.1%), while in mitosis learning the novice category was only 7 people (9.7%). Based on these findings, it can be concluded that there has been an increase in the number of students who are increasingly skilled in collaborating with students.

The determination of antioxidant and lead content of hemiparasite Dendrophthoe vitellina (F. Muell) Tiegh on Nutmeg Sabandar, Anna Helena; Kasmiyati, Sri; Djohan, Djohan
Pharmaciana Vol. 13 No. 3 (2023): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v13i3.26780

Mistletoe, a parasitic plant growing on nutmeg trees, is conventionally perceived as a parasitic entity with limited regard for its potential medicinal value. On the other hand, mistletoe has a remarkable potential source of valuable medicinal compounds, particularly in traditional healthcare, due to its secondary metabolites such as flavonoid, phenolic, ascorbic acid, and antioxidant activity. Nevertheless, both the host tree and the environment, like Pb, influence the adaptive responses of secondary metabolites. This study aimed to determine the secondary metabolites, such as flavonoids, phenolics, ascorbic acid, antioxidant activity, and Pb content in the leaves of D. vitellina and nutmeg (M. fragrans). The samples were obtained via maceration using ethanol. The spectrophotometric analysis method was used to measure several parameters, using particular reagents for phenolic compounds using Folin-Ciocalteu, flavonoids using AlCl3, and ascorbic acid using sulfosalicylic acid), antioxidant activity using DPPH, and Pb using the AAS method. The results indicated that D. vitellina possesses a higher concentration of flavonoid and phenolic compounds, followed by 3.36 ± 1.92 % (w/w) and 18.45 ± 2.35 % (w/w) respectively. Conversely, nutmeg had a significant ascorbic acid concentration of 3.99 ± 4.38 % (w/w). The significant presence of phenolics and flavonoids had a crucial role in the antioxidant activity seen in D. vitellina, which exhibited exceptionally potent antioxidant properties. All samples contain Pb, ranging from 0.00001–0.00021 % (w/w), within the maximum allowable limits. Therefore, mistletoe is notable for being a reliable and encouraging plant species that can provide a natural supply of antioxidants and be safely used in traditional medicine.

Combination of polyherbal Phyllanthus reticulatus with Zingiber officinale and Cymbopogon citratus to optimize the antioxidant capacity Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Martono, Yohanes
Pharmaciana Vol. 14 No. 1 (2024): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/pharmaciana.v14i1.28002

Currently, the public is interested in polyherbal-based foods and beverages as a source of natural antioxidants. The aim of the study is to evaluate the antioxidant properties and the phenolic and flavonoid compounds of formulations containing Z. officinale, C. citratus, and P. reticulatus (ZCP). Each sample was extracted using the maceration process in an ethanol solvent at room temperature for three 72-hour periods. There were fourteenth formulation of Z. officinale rhizome, C. citratus leaves, and P. reticulatus fruit which used Design of Expert (DoE). The DPPH method was used to determine the power of antioxidants. The flavonoid content of the extract was measured using the colorimetric method and AlCl3 reagent, while phenolics content using Folin-Ciocalteu. The formulations ZCP 1:0:0, 0:0:1, and 1:1:1 showed the antioxidant capacity in a strong categorization, with an IC50 value less than 50 µg/ml, while ZCP 0:1:0 was in a weak categorization (IC50 > 250 µg/ml). Another ZCP formulation was in a medium category. The ZCP 1:1:1 formulation was suggested as the best one for this investigation, which contains three plant samples. This formulation is interesting for further toxicity studies and in vivo testing so that it can be applied as an antioxidant-rich supplement product.

The Phytochemical and Biological Activities of Two Phyllanthus Species: Insights into Metabolit, Antioxidant and Antibacterial Activity Araújo, Otilda Fátima; Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Nugroho, Laurentius Hartanto
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 3 (2024): December 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i3.13944

Phylanthus species, including P. niruri and P. urinaria have pharmacological potential due to their rich phytochemical composition. People usually used this plant for medicinal treatments. This study aimed to compare the phytochemical and antioxidant and antibacterial activities of two Phyllanthus species. The whole of plants was extracted using maceration method with ethanol as solvent. Phytochemical content analyzed using spectrophotometer. The reagent used for each compounds that were Folin-ciocalteu for phenolics, AlCl3 for flavonoid, dimethyl sulfoxide for chlorophyll and carotenoid. Bioactivity analysis using 1,1-diphenyl-2-picrylhydrazyl method for antioxidant activity and Kirby-Bauer method for antibacterial activity. The highest flavonoid content (12.22 mg QE/gram extract) and total chlorophyll (43.2 µg/ml extract) in P. niruri while phenolic content (80.8 mg GAE/gram extract) in P. urinaria. The carotene of both Phyllanthus were similar (11.9 µg/ml extract. The IC50 values of P. urinaria (6.16 ± 0.42 μg/ml) and P. niruri (17.72 ± 0.80 μg/ml), which indicated very strong antioxidant activity. P. urinaria leaf extract had stronger inhibition against Escherichia coli than Staphylococcus aureus (>20 mm) and P. niruri leaf extract could inhibit E. coli and S. aureus bacteria at all concentrations (11-20 mm). This study found that phenolic compounds strongly influenced the antioxidant and antibacterial abilities of Phyllanthus, while chlorophyll and carotenoids had only a slight influence. These findings open up opportunities to utilize P. niruri and P. urinaria as antioxidant and antibacterial agents.

Extract Formulation of Zingiber officinale and Phylanthus reticulatus Induces Apoptosis of WiDr Colon Cancer Cells Kristiani, Elizabeth Betty Elok; Kasmiyati, Sri; Martono, Yohanes; Kachingwe, Baxter Hepburn
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.25776

People use ginger (Zingiber officinale) extensively for health purposes. Our previous studies have shown that Phyllanthus reticulatus has strong antioxidant capabilities. This study investigates the anticancer potential of ethanolic extract of Z. officinale and P. reticulatus formulations (ZP), focusing on their cytotoxicity and mechanisms against cancer cells. Samples were extracted using the maceration method. The method for determining the cytotoxicity of cells is MTT, the cell cycle is flow cytometry, apoptotic cells are flow cytometry and double staining, and caspase-8 and caspase-9 expression using the immunocytochemistry method. The analysis of caspase-8 and caspase-9 expression using the immunocytochemistry method. Among the tested formulations, the ethanolic extract of ZP_1:0 showed the most promise against WiDr colon cancer cells (IC50 value of 102.1 µg/ml). The ZP_1:0 demonstrated dose-dependent apoptosis induction in WiDr cells, showing early and late apoptosis, with minimal necrosis. The extract activates extrinsic and intrinsic apoptotic pathways in inducing cancer cell death. Compared to doxorubicin, the ZP 1:0 formulation selectively promotes apoptosis with reduced necrotic damage, suggesting the potential for reduced systemic toxicity. The study supports the potential of Z. officinale as a source of safer, plant-based anticancer agents, particularly for colon cancer, while recommending further research to identify and enhance active compounds.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.10270

THE APPLICATION OF Fe AND Cr(III) IN GROWING MEDIA AND ITS EFFECT ON PLANT GROWTH AND Cr(III) OXIDATION ON Tagetes erecta Kasmiyati, Sri
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1784

The oxidation of Cr(III) to Cr(VI) in the environment has a detrimental impact because it can change the form of non-toxic Cr(III) to Cr(VI), which is toxic to organisms. The study aimed to examine the effect of the application of iron (Fe) and trivalent chromium (Cr(III)) compounds in Tagetes erecta growing media on growth and Cr(III) oxidation. Concentrations of Cr(III) 0, 100, and 500 mg/L and Fe 0, 3, 15, and 30 mg/L were applied to the growing media of T. erecta as the model plant. The growth and accumulation of Cr(VI) in plants were measured to determine the effect of Fe and Cr(III) treatment on growth and Cr(III) oxidation. The accumulation of Cr(VI) in the roots and shoots of T. erecta increased significantly due to the addition of Fe in the growing media treated with Cr(III). The highest accumulation of Cr(VI) in the roots and shoots of T. erecta found in the treatment of Cr(III) 500 mg/L and Fe 30 mg/L, were respectively 0.092 g/L and 0.070 g/L. The addition of Fe in growing media containing Cr(III) increased plant height, root length, and shoot dry weight but decreased leaf number and root dry weight. T. erecta root biomass was more affected by the toxic impact of Fe than Cr(III). On the other hand, the inhibition of leaf formation was caused by the toxic effect of Cr(III) rather than Fe.

Co-Authors ,, Djohan Agna Sulis Krave Agustien Sri Noerwahju Alberthnico, Ferdian Andreas Binar Aji Sukmana Anggara Mahardika Anna Helena Sabandar Araujo, Otilda Fatima Ariyanto, Prasetyo Eko Betty Elok, Elizabeth Cahyaningrum, Desti Christian Cantika Glodia Debora Kristiani Rahardjo Desti Christian Cahyaningrum Dewi Prabawaningrum Djohan Djohan Djohan Djohan Djurubasa, Dani Karel Marthin Egarani, Grahita Rezhi Elizabeth B.E Kristiani Elizabeth Betty Elok Kristiani Elizabeth Bety E.K Fanny Nurwahidah, Cantika Fathiya Ulfa Nurrasyida Fatima Araujo, Otilda Gabriella L. Mataradja Hapsari Sekar Widiantari, Olive Ie, Johanes Juan Yutama Putra Indrianasari, Putri Irfan Dwidja Priyambada, Irfan Dwidja Jokosulistyo Wartanto Juliana Sarioa Kachingwe, Baxter Hepburn Kapitarauw, Agnesya Giovani Putri Cendana Kaya, Emma Sharon Admatha Keliat, Natalia Rosa Kumala Dewi L. Hartanto Nugroho Lani, Rischa Karmila Margareta Prameswati Maria Marina Herawati Monica Mangudis, Vicharisti Nabila, Nabila Natalia Rosa Keliat Novellasari, Firdiana Penina Intansari Prasgi, Henokh Christian Pratama, Dimas Seno Bagus Priyayi, Desi Fajar Purwoko, Agus Putra Ie, Johanes Juan Yutama Putri, A C. Rahardja, P M. Ramagita, Marsahanda Astri Rimanti, Audri Septina Putri Rintawati Sandradewi, Rintawati Risya Pramana Situmorang Rully Adi Nugroho Sabandar, Anna Helena Santosa S, Santosa Satria Dharmawan Kustia Dewa Seleiman, Mahmoud F. Setya Magdalena, Ajeng Shinta, Damaris Clara Siahaya, Esty Vicariat Sucahyo Sucahyo Sucahyo Susanti Pudji Hastuti Susanti Puji Hastuti Syuhuud Arumbinang Wajdi Tambunan, Roslinda Kristin Trisianna, Jessica Ayu Trisianna Vincentia Irene Meitiniarti Vini Kurnia Ramadhani Wahyu Hari Kristiyanto Widyaningrum, Indy Yohanes Martono Yolanda Halim, Vivian

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