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Desain dan Optimasi Primer Gen Pengkode MRPA Trypanosoma evansi dan Penerapan pada Pembelajaran Biologi Molekuler Nuryady, Moh. Mirza; Husamah, H.; Miharja, Fuad Jaya; Hindun, Iin; Patmawati, P.
Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Vol. 4 No. 2: July 2020
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/e-saintika.v4i2.217

Abstract

Penelitian molekuler untuk menemukan gen pengkode resistensi Multidrug Resistance Prtotein A (MRPA) T. evansi dan perbanyakan gen secara Polimeration Chain Reaction (PCR)  masih sedikit dilakukan  dan sangat penting untuk dipahami oleh mahasiswa calon guru biologi.  Kajian ini bertujuan untuk menganalisis proses desain dan optimasi primer untuk gen target MRPA T. evansi yang dapat digunakan sebagai sumber belajar mahasiswa pendidikan biologi. Penelitian ini merupakan penelitian deskriptif mengenai tahapan mendesain primer secara online, optimasi primer secara laboratorium serta kajian mengenai pentingnya penerapan hasil studi ini dalam pembelajaran. Hasil penelitian menunjukkan terdapat tiga desain primer yang memenuhi syarat, selanjutnya dari tiga primer tersebut hasil optimasi di laboratorium menunjukkan hanya terdapat dua primer yang menunjukkan hasil yang baik dan dapat digunakan untuk penelitian amplifikasi gen MRPA T. evansi, yaitu primer pertama (F1’, R1’) dan primer kedua (F2’, R2’). Hasil kajian desain dan optimasi primer ini menunjukkan bahwa mahasiswa pendidikan biologi sangatlah penting untuk memahami konsep terkait dengan pekerjaan molekuler seperti mendesain dan optimasi primer, dikarenakan mereka memiliki tuntutan untuk menjadi seorang calon pendidik atau sebagai calon peneliti dimasa depan.Design and Optimization of Trypanosoma evansi MRPA Primer Coding Genes and Application to Molecular Biology LearningAbstractMolecular research to find Multidrug Resistance Prtotein A (MRPA) resistance coding genes and gene propagation by Polimeration Chain Reaction (PCR) is still little done and is very important to be understood by prospective biology teacher students. This study aims to analyze the design and primary optimization process for the T. evansi MRPA target gene that can be used as a learning resource for biology education students. This research was a descriptive study to described the step of primer design and optimization due to the importance of this steps to be applied as learning source. The results showed that there were 3 primer designs that qualified, then after the optimizing step there were only two primers that showed a good result, the first primer (F1, R1) and second primer (F2', R2). The results of this study showed the importance of biology education students to understand the concepts related to molecular work because in the future they are not only become prospective educators, they also have demands as prospective researchers.
Exploration of Environmental Care Attitudes in Students Ar-Rohmah Islamic Boarding School Malang Based on the New Ecological Paradigm Instrument Hidayati, Dewi Arif; Husamah, H.; Fatmawati, Diani; Miharja, Fuad Jaya; Fauzi, Ahmad
Jurnal Penelitian dan Pengkajian Ilmu Pendidikan: e-Saintika Vol. 4 No. 3: November 2020
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/e-saintika.v4i3.277

Abstract

This study aimed to determine the attitude of concern for the environment among the students at Pondok Pesantren Ar-Rohmah Malang. This research was an ex post facto research where the researcher reveals and describes the facts about the environmental care attitude of the students at Ar-Rohmah Islamic Boarding School Malang. The population in this study were all students of Ar-Rohmah Senior High School Malang. The sample in this study were students of class 10, 11, and 12 SMA Senior High School Ar-Rohmah Malang who were randomly selected with the number of students in each class as many as 35 students and total as many as 210 students. The results showed that in general, the students of Ar-Rohmah Senior High School Malang tend environmental care / pro-environmental attitudes rather than social paradigm attitudes. Class differences indicate a difference in the percentage of caring attitudes towards the environment. If we look at each item, based on the percentage of responses to the NEP instrument, the students of AR-Rohmah Senior High School Malang is not understood holistically on several materials such as the balance of ecosystems, the right of natural resources not to be exploited continuously, and the obligation of humans to protect the environment and cultivate it wisely.
The relationship between environmental ethics literacy and the caracteristics of environmental awareness of biology pre-service teachers Hudha, Atok Miftachul; Aini, Firda; Miharja, Fuad Jaya
Biosfer: Jurnal Pendidikan Biologi Vol. 17 No. 2 (2024): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/biosferjpb.37672

Abstract

Behavior that damages the environment can be changed through knowledge, because knowledge can influence a person's attitude in protecting theenvironment. This research aims to analyze the relationship between environmental ethics literacy and environmental awareness in prospective biology teacher students. The research was conducted on Biology Education students at "X" University, Malang City. The research sample was taken randomly from the population and the sample in this study was 74 prospective biology teacher students in semester V of the 2020/2021 academic year, and semester VII of the 2019/2020 academic year. Sample selection was based on all students who had taken and passed ethics andecology courses. The instruments used to collect data were test questions and attitude observation sheets with Likert scales and Guttman scales. Data were analyzed using the Pearson Product Moment correlation test with data processing using the SPSS 24 for Windows program. Theresearch results show that (1) the environmental ethics literacy of prospective biology teacher students is in the medium category; (2) environmental awareness of prospective biology education teacher students in the good category; (3) there is a relationship betweenenvironmental ethical literacy and environmental awareness character, because the results of correlation analysis with the Pearson ProductMoment correlation show a significance value of 0.000 < 0.05. The results of this research show that environmental ethics literacy has a positiverelationship with environmental awareness, so to foster environmental awareness it is necessary to continuously increase environmental ethics literacy.
Teachers’ assisting in designing innovative learning based on local advantages through STEM hands-on activities Miharja, Fuad Jaya; Nuryady, Moh. Mirza; Putri, Aura Febiola Afi; Agustina, Jihan Aurelia
Jurnal SOLMA Vol. 13 No. 3 (2024)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v13i3.15885

Abstract

Background: Keterlibatan aktif dalam pembelajaran mencakup eksplorasi konsep, dengan memastikan kontekstualisasi melalui isu-isu dan keunggulan lokal. Namun, saat ini isu lokal sering hanya digunakan sebagai apersepsi di awal pembelajaran, dan guru terkendala dalam implementasinya. Oleh karena itu, dilakukan pendampingan inovasi pembelajaran STEM pada guru SMA Muhammadiyah 3 Batu. Metode: FGD dan Workshop, Pengambilan data menggunakan survey. Hasil: Aktivitas pengabdian ini menghasilkan empat model taman vertikal dengan memenuhi tiga kriteria yaitu kemenarikan dan fungsionalitas desain, kemudahan perawatan, dan estetika komposisi tanaman. Kesimpulan: Aktivitas pendampingan ini dapat memberi pengalaman nyata bagi guru dalam menginovasi desain pembelajaran berdasarkan permasalahan kontekstual di sekitar siswa.
IN SILICO AND IN VITRO ANALYSIS OF BLASHV AND BLATEM GENE PRIMERS Khoiro, Aisha Azaria Nisa’ul; Purwanti, Elly; Miharja, Fuad Jaya; Ariesaka, Kiky Martha; Nuryady, Moh. Mirza
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 11 No. 2 (2025): Biolink February
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v11i2.13830

Abstract

Bacterial pollution that occurs in the environment causes an increase in disease and antibiotic use, which leads to resistance. This study analyzes antibiotic-resistant blaSHV and blaTEM genes in silico and in vitro. Antibiotic-resistant bacterial isolates on ESBL will be carried out PCR and electrophoresis. The type of research used is descriptive observational research conducted from April to September 2024. The sampling technique used was random sampling. Primer blaSHV with sequence F' AGGATTGACTGCCTTTTTG and R' ATTTGCTGATTTCGCTCG, while primer blaTEM has sequence F' ATCAGCAATAAACCAGC and R' CCCCGAAGAACGTTTTC. Data analysis was carried out by analyzing in silico results obtained from the NCBI website and observing the visualization results of PCR amplification indicated by the presence of DNA bands.  In silico results showed 25 organisms attached to blaSHV and 29 organisms to blaTEM. In vitro results shown by electrophoresis visualization showed blaSHV amplicons measuring 392 bp with an annealing temperature of 50.5°C, and blaTEM measuring 517 bp with an annealing temperature of 42°C. The success rate of PCR was shown by clear and specific amplification of both genes, indicating that the PCR method performed was effective in detecting blaSHV and blaTEM genes in ESBL antibiotic-resistant bacterial isolate samples.  
Upaya peningkatan kualitas pembelajaran IPA di MTs Muhammadiyah 1 Malang Hindun, Iin; Wahyuni, Sri; Miharja, Fuad Jaya; Mashuri, Mashuri; Nurwidodo, Nurwidodo; Husamah, Husamah
JURNAL WIDYA LAKSANA Vol 9 No 1 (2020)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.307 KB) | DOI: 10.23887/jwl.v9i1.21463

Abstract

This assistance aims to improve the quality of science learning in MTs Muhammadiyah 1 Malang. This assistance is motivated by the limitations of LS assistance services from tertiary institutions to elementary and secondary schools. Whereas learning innovations such as LS really need to be disseminated, maintained, strengthened, and improved in their field implementation so that the achievement of quality in learning is guaranteed. Assistance is carried out with the method of socialization, implementation, monitoring, evaluation and follow-up. Assistance was followed by a group of science teachers in Matsamutu and was conducted for one semester. The results of the mentoring, the socialization phase included the Matsamutu LS team in the MEPS, conducted socialization and assisted teachers in preparing lesson plans for 2 lessons, carried out an open plan with the model teacher from the school, implemented learning reflections and documented the results of the mentoring implementation and compiled collaborative publication articles. . It can be concluded that the assistance has provided benefits in increasing understanding of the procedures, principles of implementation, and scientific work resulting from the implementation of the LS
Analisis kebutuhan ruang terbuka hijau sebagai penyerap emisi gas karbon di kota dan kawasan penyangga Kota Malang Fuad Jaya Miharja; Husamah Husamah; Tatag Muttaqin
Jurnal Pengelolaan Lingkungan Berkelanjutan (Journal of Environmental Sustainability Management) JPLB, Vol 2, No 3 (2018)
Publisher : Badan Kerjasama Pusat Studi Lingkungan (BKPSL) se-Indonesia bekerjasama dengan Pusat Penelitian Lingkungan Hidup IPB (PPLH-IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36813/jplb.2.3.165-174

Abstract

Pembangunan ruang terbuka hijau (RTH) di Malang diperlukan sebagai penyeimbang emisi gas CO2 dari aktivitas penduduk dan konsumsi bahan bakar kendaraan yang semakin tinggi. Penelitian ini bertujuan untuk menganalisis luasan RTH dan kemampuan serap CO2 serta besarnya emisi CO2 yang dilepaskan oleh aktivitas respirasi dan penggunaan bahan bakar minyak. Penelitian ini merupakan jenis deskriptif kuantitatif. Area pengambilan data dalam penelitian ini meliputi Kota Malang, Kota Batu dan Kabupaten Malang. Metode deskriptif digunakan untuk menggambarkan kondisi yang ada di area penelitian. Metode kuantitatif digunakan untuk menentukan luasan dan kemampuan RTH yang ada saat ini untuk menyerap CO2 dan besarnya CO2 yang dilepaskan. Total emisi gas CO2 yang dihasilkan sebesar 535.429 ton/tahun. Kebutuhan luas RTH berdasarkan jumlah emisi sebesar 9.139 ha dengan luasan area RTH yang tersedia saat ini sebesar 13,09 ha sehingga dibutuhkan 9.126 ha lahan tambahan untuk menyeimbangkan kebutuhan atas RTH.
Peningkatan Kapasitas Guru Sekolah Piloting dalam Lesson Study for Learning Community di SMP Muhammadiyah 1 Malang Fuad Jaya Miharja; Husamah Husamah; Masrudi Masrudi
Sasambo: Jurnal Abdimas (Journal of Community Service) Vol. 2 No. 3: October 2020
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/sasambo.v2i3.311

Abstract

Program pendampingan Lesson Study for Learning Community (LSLC) di Sekolah Muhammadiyah telah memasuki tahun ketiga dan terus dilakukan penyempurnaan program. Dalam pelaksanaannya ketersediaan dokumentasi pembelajaran masih menjadi tantangan yang perlu diselesaikan, karena ketersediaan dokumentasi empiric merupakan aspek fundamental dalam melakukan refleksi dan rencana tindaklanjut. Pendampingan dilaksanakan selama bulan Maret – Juli 2020 di SMP Muhammadiyah 1 Malang. Metode pelaksanaan yang mengacu kepada analisis situasi program-program yang disepakati bersama dengan pihak sekolah adalah diskusi penyamaan persepsi, lokakarya, pendampingan, dan monitoring evaluasi. Kegiatan pendampingan telah menginisiasi guru dalam memaknai LSLC sebagai komunitas belajar dan memahami teknik dasar pelaksanaan observasi secara komprehensif
Pelatihan Pengembangan Soal Berbasis HOTS bagi Guru Sekolah Dasar Fuad Jaya Miharja; Ahmad Fauzi; Atok Miftachul Hudha
Sasambo: Jurnal Abdimas (Journal of Community Service) Vol. 4 No. 2: May 2022
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/sasambo.v4i2.677

Abstract

Pembelajaran daring selama pandemi Covid-19 diindikasi berdampak pada menurunnya keterampilan berpikir kritis siswa, terlebih kecenderungan guru kesulitan dalam mengembangkan soal berbasis HOTS. Pengabdian ini bertujuan melakukan melatih guru sekolah dasar dalam mengembangkan soal berbasis HOTS dalam rangka meningkatkan keterampilan berpikir kritis siswa selama pembelajaran daring. Metode pelaksanaan pengabdian ini mengacu pada analisis situasi dengan pihak sekolah, meliputi diskusi, sosialisasi, pelatihan, workshop, dan praktek langsung. Pengabdian dilakukan selama bulan Juli – September 2021, melibatkan 24 orang guru. Instrumen pengukuran berpikir kritis yang digunakan dalam pengembangan soal HOTS merujuk kepada penelitian Zubaidah. Hasil pengabdian menunjukkan bahwa pengembangan soal berbasis HOTS perlu dikembangkan secara tersistem dari perencanaan hingga evaluasi. Lebih lanjut, hasil pengabdian juga memberikan perspektif yang lebih mendalam tentang bagaimana membelajarkan HOTS dengan menggunakan model pembelajaran inovatif. HOTS-Based Question Development Training for Elementary School Teachers Online learning during the Covid-19 pandemic is indicated to impact the decline in students' critical thinking skills, especially for teachers to find it challenging to develop HOTS-based questions. This service aims to train elementary school teachers to develop HOTS-based questions to improve students' critical thinking skills during online learning. Implementing this service refers to situation analysis with the school, including discussions, socialization, training, workshops, and hands-on practice. The service is carried out from July to September 2021, involving 24 teachers. The critical thinking measurement instrument used in developing HOTS questions refers to Zubaidah's research. The service results show that the development of HOTS-based questions needs to be developed systematically from planning to evaluation. Furthermore, the service results also provide a deeper perspective on how to teach HOTS using innovative learning models.