Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : Jurnal Biologi Tropis

In Vitro Germination of Dumbaya Seeds (Momordica cochinchinensis (Lour.) Spreng: A Unique Medicinal Plant of Gorontalo Devi Bunga Pagalla; Jusna Ahmad; Miftahul F. Adudu; Adilah Nidaulhasanah; Fitria H.Ys. Adju; Evita Moni Damayanti
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.5798

Abstract

"Dumbaya (Momordica cochinchinensis (Lour.) Spreng) is one of the plant species believed by the Gorontalo community to be a traditional remedy for various ailments such as inflammation, liver disorders, spleen issues, hemorrhoids, bruising, and infected wounds. Currently, the dumbaya plant is difficult to find and is even considered nearly extinct by the Gorontalo community. This is due to the challenging conventional cultivation of dumbaya plants from seeds. Dumbaya seeds have a hard, stone-like texture, making germination in the wild difficult and requiring special treatment. Regeneration of dumbaya can be achieved through tissue culture techniques, replacing the conventional cultivation system.Through tissue culture, dumbaya seeds are grown in specialized planting media and controlled environments. Based on observations, the propagation of dumbaya plants using in vitro culture techniques has proven successful and effective as a solution for propagating dumbaya seedlings. Dumbaya seeds begin to germinate two weeks after planting (2 WAP). The grown dumbaya seedlings can then be used as explants for further dumbaya propagation by inducing callus formation."
Effect of BAP and NAA on Callus Emergence Time of Dumbaya Young Leaf Explants in Vitro Walangadi, Farhana Rahmatia; Ahmad, Jusna; Pagalla, Devi Bunga; Kandowangko, Novri Youla; Febriyanti, Febriyanti
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.9045

Abstract

Momordica cochinchinensis, locally known as Dumbaya in Gorontalo Province, is a traditional medicinal plant with underutilized potential due to the extremely hard morphology of its seed coat. This physical barrier limits the penetration of water, air, and nutrients, thereby reducing the success rate of seed germination and plant propagation. The challenge of propagating plants that are difficult to reproduce sexually, such as dumbaya, can be addressed through various approaches, one of which is asexual reproduction using tissue culture techniques. This study aims to address propagation constraints by applying tissue culture techniques supplemented with Plant Growth Regulators (PGRs), specifically BAP (Benzyl Amino Purine) and NAA (Naphthalene Acetic Acid), which are critical factors in successful in vitro plant regeneration. The combination of Benzyl Amino Purine (BAP) and Naphthalene Acetic Acid (NAA) is a commonly used Plant Growth Regulator (PGR) for inducing callus formation and organogenesis, where BAP is effective in stimulating shoot formation, while NAA plays a role in root induction. The research employed an experimental method using a Completely Randomized Design (CRD) with five treatment levels: H0: MS + 0 ppm NAA + 0 ppm BAP; H1: MS + 1.5 ppm NAA + 0.5 ppm BAP; H2: MS + 3.0 ppm NAA + 0.5 ppm BAP; H3: MS + 1.5 ppm NAA + 1.0 ppm BAP; and H4: MS + 3.0 ppm NAA + 1.0 ppm BAP. Each treatment was replicated three times. The results showed that treatment H1 produced the earliest callus formation, with an average callus initiation time of 7 days after planting.
Comparison of Anatomical Structure of Maize (Zea mays L.) Plant of Gorontalo Local Variety Pulut and Momala Ahaya, Cindi; Kandowangko, Novri Youla; Febriyanti, Febriyanti; Ahmad, Jusna; Pagalla, Devi Bunga
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8483

Abstract

One of the primary agricultural commodities that contributes significantly to global food security is maize (Zea mays L.), a monoecious cereal plant of the Poaceae family with distinct but identical male and female blooms. Local cultivars like Pulut and Momala in Gorontalo have exceptional promise that requires further study, particularly with regard to their anatomical makeup. This study Comparing the two types' stem and leaf anatomical features, including vascular tissue (xylem and phloem), epidermis, and stomata, is the goal of this study. A qualitative descriptive approach was used to make the observations, which were subsequently evaluated and shown as a comparison table. The results showed that at the age of 30 and 40 HST, the network of vessels (xylem and phloem) and epidermis on the stems and leaves of Pulut and Momala corn varieties reflected a development pattern that supported the transportation of water, minerals, and nutrients. The number of stomata in Pulut varieties decreased due to less than optimal growth, while Momala varieties showed an increase in the number of stomata which was influenced by the growth period with environmental conditions suitable for corn plants.