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All Journal MPI (Media Pharmaceutica Indonesiana) Jurnal Kesehatan Tambusai Bioscientist : Jurnal Ilmiah Biologi Jurnal Farmasi Sains dan Praktis Jurnal Ilmiah Sains Jurnal Pharma Bhakta
Nadia Pramasari
Institut Ilmu Kesehatan Bhakti Wiyata
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Penetapan Kadar dan Uji Stabilitas Senyawa Stigmasterol pada Obat Herbal Peluruh Batu Ginjal yang Mengandung Ekstrak Keji Beling (Strobilanthes crispus) menggunakan Metode KCKT Purwani, Asih Imulda Hari; Aryantini, Dyah; Kurniawati, Evi; Wahyuni, Dwi; Pramasari, Nadia
MPI (Media Pharmaceutica Indonesiana) Vol. 8 No. 1 (2026): JUNE
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v8i1.7774

Abstract

Nefrolitiasis atau batu ginjal merupakan salah satu gangguan saluran kemih yang prevalensinya terus meningkat di berbagai negara, termasuk Indonesia. Penggunaan tanaman obat sebagai terapi alternatif telah banyak dilakukan, salah satunya Strobilanthes crispus (keji beling) yang secara empiris memiliki efek peluruh batu ginjal. Efektivitas tanaman ini berkaitan dengan keberadaan senyawa aktif salah satunya adalah stigmasterol. Penetapan senyawa marker menjadi aspek penting dalam proses standardisasi untuk menjamin kualitas dan konsistensi produk herbal. Metode analisis berbasis kromatografi, seperti Kromatografi Cair Kinerja Tinggi (KCKT), banyak digunakan dalam identifikasi, uji stabilitas dan penetapan kadar senyawa marker. Hasil penelitian menunjukkan stabilitas senyawa aktif stigmasterol pada produk kapsul herbal dalam rentang waktu 0–24 jam, terdapat penurunan area pada larutan standar kurang dari 2% dan pergeseran waktu retensi (tR) juga kurang dari 2%. Hasil pengujian diperoleh persen kadar stigmasterol sebesar 16,07% dengan nilai Vxo 3,48%. Nephrolithiasis, or kidney stone disease, is one of the most common urinary tract disorders, with a prevalence that continues to increase worldwide, including in Indonesia. The use of medicinal plants as alternative therapies has been widely explored, one of which is Strobilanthes crispus, which is empirically known for its diuretic and lithotriptic effects. The effectiveness of this plant is associated with the presence of bioactive compounds, particularly stigmasterol. The determination of marker compounds is a crucial aspect of the standardization process to ensure the quality and consistency of herbal products. Chromatography-based analytical methods, such as High-Performance Liquid Chromatography (HPLC), are commonly employed for compound identification, stability testing, and quantification of marker compounds. The results of this study demonstrated that stigmasterol exhibited good stability in herbal capsule formulations within a time range of 0–24 hours, with a decrease in standard solution area of less than 2% and a retention time (tR) shift was below 2%. The stigmasterol content was determined to be 16.07%, with a Vxo value of 3.48%.
Development and Validation of a TLC-Densitometric Method for Quantification of Quercetin in Smallanthus sonchifolius Extract Nurhayati, Rachma; Adhelia, Vanessa; Purwani, Asih Imulda Hari; Pramasari, Nadia; Shoviantari, Fenita
MPI (Media Pharmaceutica Indonesiana) Vol. 8 No. 1 (2026): JUNE
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v8i1.8736

Abstract

This study aimed to develop and validate a thin-layer chromatography (TLC)–densitometric method for the quantitative determination of quercetin in insulin leaves (Smallanthus sonchifolius). Dried insulin leaves were extracted using the maceration method with 70% ethanol as the extraction solvent. Quercetin analysis was carried out on silica gel GF254 plates as the stationary phase, using a mobile phase consisting of n-butanol, acetic acid, and water in a ratio of 4:1:5 (v/v/v). A sample volume of 4 μL was applied onto the plate and scanned at a wavelength of 284 nm using a densitometer. Method validation was performed by evaluating linearity, selectivity, accuracy, and precision parameters. The validation results showed a good linear relationship between concentration and peak area with a correlation coefficient (r) of 0.9844. The method exhibited good selectivity with a resolution value of 2.40 and peak purity of 0.99. Accuracy values ranged from 101.4% to 102.5%, while precision testing produced a relative standard deviation value of 3.0%. The average quercetin content in insulin leaves was determined to be 0.191% (w/w). These findings demonstrate that the validated TLC–densitometric method is reliable and suitable for routine quantitative analysis of quercetin in herbal raw materials for quality control purposes.
Co-Authors Ade Giriayu Anjani Adhelia, Vanessa Anikasari, Erni Anna Wahyuni Widayanti Asih Imulda Hari Purwani Dwi Wahyuni Dyah Aryantini, Dyah Evi Kurniawati, Evi Fenita Shoviantari, Fenita Handaru, Theo Aryaputra Cahya Hari Untarto Swandono Hayati, Rachma Nur Hesturini, Asih Imulda Kusumaratni, Dyah Ayu Marcelina, Ananda Marhenta, Yogi Bhakti Ninis Yuliati, Ninis Putri, Sherlyna Rizkiya Rachma Nurhayati Rosa Hesturini Rosa Juwita Hesturini Rumwarin, Yuliana Sholehuddin, Sultan Satrio Sugiyartono