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All Journal Jurnal Bios Logos BULETIN OSEANOGRAFI MARINA Protobiont: Jurnal Elektronik Biologi SEMIRATA 2015 Majalah Obat Tradisional Jurnal Sains & Teknologi Lingkungan BIOMA Jurnal Bioteknologi & Biosains Indonesia (JBBI) JFMR (Journal of Fisheries and Marine Research) JURNAL PENGABDI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Jurnal Labora Medika Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Entomologi Indonesia Jurnal Riset Biologi dan Aplikasinya Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Jurnal Borneo Akcaya : Jurnal Penelitian dan Pengembangan Pelayanan Publik Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Perikanan Indonesia
Diah Wulandari Rousdy
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Tanjungpura, Pontianak, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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Kerusakan Histologi Insang Ikan Sapu-sapu (Pterygoplichthys pardalis) setelah Paparan Merkuri (HgCl2) Siti Anikha Idzni; Diah Wulandari Rousdy; Junardi Junardi
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 37, No 3 (2020)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2020.37.3.1137

Abstract

Mercury is a heavy metal that can undergo biomagnification in the tissue of aquatic organisms, including accumulating in the gills of fish. The main accumulation of mercury occurs in organisms that live in polluted waters, one of which is the suckermouth cat fish (Pterygoplichtys pardalis). The purpose of this study was to determine the histological damage of Pterygoplichtys pardalis gills against mercury (HgCl2) exposure. This study used a Completely Randomized Design consisting of six treatments and three replications. The treatments consisted of control; 0.01 0.02; 0.04; 0.08; 0.16 ppm HgCl2 concentration. The results of the study obtained forms of gill damage in the form of edema, hyperplasia, lamella fusion and epithelial lifting. The highest level of damage occurred at 0.16 ppm HgCl2 concentration in the form of a reduction in secondary lamella structure. Exposure to mercury causes damage to P. pardalis fish gills
Liver Histopathology of Mice Induced by Paracetamol on Administration of Methanol Extract of Laban Tree Bark (Vitex pubescens Vahl.) Wiwit Anggraini; Diah Wulandari Rousdy; Elvi Rusmiyanto Pancaning Wardoyo
Bioma : Jurnal Ilmiah Biologi Vol 12, No 1: April 2023
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v12i1.11664

Abstract

Paracetamol metabolism takes place in the liver mediated by CYP450 enzymes and generates NAPQI radicals. The NAPQI radical is highly electrophilic to hepatocyte biomolecules and is therefore capable of inducing liver damage. Laban tree bark (V. pubescens) methanol extract is known to contain antioxidant compounds of flavonoids, saponins, and tannins with an IC50 value of 19.83 g/ml. This study aims to determine the optimal dose of extract that can improve the liver microanatomy of mice induced by toxic dose of paracetamol (TDP). This study used 30 male Swiss strain mice with a body weight of 20-30 g. The treatments given consisted of negative control (paracetamol 105 mg kg-1), positive control (vitamin E 46.8 mg kg-1), and laban extract at a dose of 75; 150; 300; 600 mg kg-1 BW. Liver microanatomy parameters measured included hepatocyte diameter (19.50±0.21 µm), sinusoids (4.39±0.23 µm), and central vein (36.84 ± 1.79 µm). Data were analyzed using one-way ANOVA and Duncan's Multiple Range Test further test was then presented in the form of mean ± standard deviation. The results showed that extract at a dose of 600 mg kg-1 had hepatocyte, sinusoid, and central vein diameters that were not significantly different (p>0.05) with vitamin E.
Pertumbuhan dan Densitas Populasi Larva Hermetia illucens (L.) pada Media Tumbuh Limbah Nanas dan Dedak Padi Cesaria Anggun Kinanty; Diah Wulandari Rousdy; Firman Saputra
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.7637

Abstract

The presence of Hermetia illucens larvae in waste management systems is very beneficial because of their ability to degrade waste. The growth of Hermetia illucens larvae depends on the adequacy of nutrients contained in the growth media such as protein and fat. The purpose of this study was to determine the growth, density and nutritional correlation of pineapple and rice bran media on Hermetia illucens larvae. The study used a completely randomized design consisting of 5 treatments, namely: 1) 100% pineapple waste; 2) 75% pineapple waste and 25% rice bran; 3) 50% pineapple waste and 50% rice bran; 4) treatment of pineapple waste 25% rice bran 75%; and 5) 100% rice bran. Each treatment consisted of 5 replications. Hermetia illucens larvae were reared for 21 days in organic waste media. The results showed that Hermetia illucens larvae grew optimally on a combination of 75% pineapple waste and 25% rice bran, namely an average length of ±15.298 mm and an average weight of ±11.644 g (sig <0.05), while the population density of the larvae showed no significant difference between treatments. with a density of 0.023-0.024 fish/cm3. The results of Pearson correlation analysis showed that the nutrient content of the media such as protein, fat, ash, moisture content and crude fiber had an effect on the length and weight of Hermetia illucens larvae, but had no effect on the larval population density.
Immune Responses of Wistar Rat (Rattus novergicus) on Adduction of Humid Acid from Borneo Peat Soil Diah Wulandari Rousdy; Rahmawati Rahmawati; Rikhsan Kurniatuhadi
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7499

Abstract

Peat soil is a type of soil that dominates the island of Borneo. Typical compounds in peat soil is humic acid. Various in vitro studies performed have shown peat subtropical humic compounds can stimulate the immune system. However, in vivo study on animal has not been done. This study aimed to determine the effect of humic acid extracted from peat soil of Borneo against the immune system, both of non-specific and specific immunity Wistar rats (Rattus novergicus). Research using a completely randomized design with five treatments and five replicates, the normal controls, a positive control (isoprinosine), humic acid 125; 250; 500 mg/kg. Humic acid was administered orally for 10 days. The results showed humic acid adduction did not significantly affect levels of hemoglobin, erythrocytes and hematocrit. Humic acid adduction of 125 mg/kg significantly affects the total leukocyte count and differential leukocyte. Humic acid 125 mg/kg also showed increased phagocytic index better than normal controls. All humic acid treatments do not provide a significant effect on the total amount of antibody. The results of this study can be used for the development of Borneo tropical peat resources as natural imunostimulant.How to CiteRousdy, D. W., Rahmawati, R. Kurniatuhadi, R. (2016). Immune Responses of Wistar Rat (Rattus novergicus) on Adduction of Humid Acid from Borneo Peat Soil. Biosaintifika: Journal of Biology Biology Education, 8(3), 401-406. 
Karakter pintu masuk sarang kelulut (Apidae: Meliponinae) di Kawasan Konservasi Resort Belaban Taman Nasional Bukit Baka Bukit Raya Kalimantan Barat: Nest entrances characters of stingless bees (Apidae: Meliponinae) in Conservation Area of Belaban Resort, Bukit Baka Bukit Raya National Park West Borneo Marsianus Marthin Rivaldy; Kustiati Kustiati; Diah Wulandari Rousdy; Hendro Priyandono
Jurnal Entomologi Indonesia Vol 20 No 1 (2023): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.1.67

Abstract

Information on the character of the stingless bee nest entrance (Hymenoptera: Apidae: Meliponinae) as a characteristic for identifying stingless bee species has never been carried out in depth. The purpose of this study was to determine the morphological characteristics of the nest entrance that differentiate between stingless bee species found in the Belaban Resort conservation area, Bukit Baka Bukit Raya National Park. The research was conducted on March, 16–26 2022. The method used in this study was the cruising method by following the direction of the Belaban Resort interpretation route. Data analysis was performed with PAST 4.03 software. Based on sampling and species identification, 32 nest entrances were found from 7 stingless bees species, namely Homotrigona fimbriata (Smith), Pariotrigona pendleburyi (Schwarz), Tetragonilla collina (Smith), Tetragonula iridipennis Smith, T. melanocephala (Gribodo), T. melina (Gribodo), and Tetrigona apicalis (Smith). PCA results show that the most prominent morphometric characters in PC 1 (eigenvalue = 4.89; % variance = 44.51%) which can be used as identifiers for each stingless bee nest entrance width of the nest entrance opening cavity  (0.66), color (0.78), sun exposure (0.85), and shape of nest entrance funnel (0.57). This research shows the variation of meristic and morphometric characters of stingless bee nest entrance. However, identification for stingless bee species based on nest entrance characters can't be used validly so it only as a provisional estimate.
Ekstrak Biji Kesumba Keling (Bixa orellana L.) sebagai Pewarna Alami Sediaan Jaringan Ikan Nila (Oreochromis niloticus) Filda Rahmawati; Kesha Purwaning Sari; Nurul Huda; Diah Wulandari Rousdy
JURNAL BIOS LOGOS Vol. 13 No. 3 (2023): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v13i3.51905

Abstract

Penggunaan pewarna sintetis dalam histologi dapat memberikan dampak buruk bagi kesehatan dan lingkungan. Keberadaan pewarna alami menjadi alternatif permasalahan tersebut, salah satunya ekstrak biji kesumba keeling (Bixa orellana L.). Riset ini dilakukan bertujuan untuk mengetahui konsentrasi ekstrak biji kesumba keling yang memberikan pewarnaan jaringan terbaik pada gambaran histologi organ hepar, insang, dan ginjal ikan nila (Oreochromis niloticus). Riset dilakukan selama empat bulan dan terbagi menjadi 4 tahapan, yaitu tahap aklimatisasi ikan, ekstraksi biji kesumba dengan pelarut etanol 70%, pembuatan preparat dengan metode parafin, dan pengamatan struktur histologis jaringan ikan. Konsentrasi ekstrak biji kesumba yang digunakan adalah 35%, 50% dan 70%. Masing-masing konsentrasi pewarna diulang sebanyak 5 preparat. Hasil pengamatan gambaran histologis dilakukan secara deskriptif. Hasil penelitian menunjukkan ekstrak biji kesumba mampu memberikan warna merah muda pada sitoplasma sel dan dapat menggantikan pewarna eosin sehingga berperan sebagai counterstaining terhadap pewarna hematoksilin. Akan tetapi ekstrak biji kesumba tidak memberikan warna pada inti sel sehingga tidak dapat menggantikan pewarna hematoksilin. Konsentrasi ekstrak biji kesumba yang memberikan warna sediaan jaringan paling baik adalah konsentrasi 50%.
KETERAMPILAN PEMBUATAN KAIN ECOPRINT BAGI GURU PENGGERAK DI KOTA PONTIANAK Diah Wulandari Rousdy; Kustiati; Dwi Gusmalawati; Rahmawati
Jurnal Abdimas Ilmiah Citra Bakti Vol. 5 No. 2 (2024)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v5i2.2349

Abstract

Guru Penggerak merupakan salah satu program unggulan Kementerian Pendidikan yang memerlukan keluasan pengetahuan dan keterampilan para guru. Peningkatan pengetahuan dan keterampilan Guru Penggerak dalam menyampaikan ilmunya, khususnya bidang Ilmu Pengetahuan Alam dapat dilakukan melalui pemberian keterampilan ecoprinting. Ecoprint merupakan teknik pemberian motif kain menggunakan bahan alami tumbuh-tumbuhan. Mitra kegiatan pengabdian adalah Guru Penggerak Angkatan I Kota Pontianak. Kegiatan pengabdian ini bertujuan memberikan alternatif pembelajaran bagi guru dalam menjelaskan materi IPA, terutama pembelajaran mengenai tumbuhan. Metode pengabdian yang digunakan pada pelatihan ini meliputi penyampaian teori dan praktek pembuatan kain ecoprint secara langsung. Pelaksanaan PKM mandiri dilakukan pada bulan Juli 2022. Peserta kegiatan adalah guru penggerak angkatan pertama dari Kota Pontianak yang berjumlah 13 orang dengan asal sekolah yang bervariasi mulai dari TK hingga SMA. Materi kegiatan yang diberikan meliputi pengenalan prinsip ecoprinting, pengenalan alat, bahan kimia, media, jenis dan karakter daun serta kekurangan dn kelebihan ecoprint. Hasil evaluasi kuisioner diperoleh 38,5% peserta menyatakan kegiatan ini dapat menambah ilmu pengetahuan dan 61,5% peserta akan mengaplikasikannya bersama dengan siswa di sekolah masing-masing. Seluruh peserta (100%) menyatakan bahwa kegiatan ini sangat bermanfaat dan memberikan inspirasi dalam memberikan materi kepada siswa sekolah.
STRUKTUR KOMUNITAS MAKROALGA DI PERAIRAN TEMAJUK KECAMATAN PALOH KALIMANTAN BARAT: COMMUNITY STRUCTURE OF MACROALGAE IN TEMAJUK WATERS PALOH DISTRICT WEST KALIMANTAN Safitri, Ikha; Warsidah, Warsidah; Sofiana, Mega Sari Juane; Gusmalawati, Dwi; Rousdy, Diah Wulandari; Aswandi, Aswandi
JFMR (Journal of Fisheries and Marine Research) Vol. 7 No. 1 (2023): JFMR on March
Publisher : Faculty of Fisheries and Marine Science, Brawijaya University, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2023.007.01.7

Abstract

Makroalga merupakan salah satu potensi sumberdaya laut yang secara ekologis berperan dalam meningkatkan produktivitas primer perairan, bioremediasi berbagai jenis polutan, penyedia oksigen, sumber makanan, dan menyediakan habitat berbagai jenis biota akuatik lainnya. Secara ekonomi, makroalga juga potensial untuk dikembangkan karena mengandung berbagai senyawa bioaktif yang dapat dimanfaatkan di berbagai bidang industri. Keanekaragaman jenis dan kelimpahan makroalga sangat dipengaruhi oleh parameter fisika-kimia perairan. Desa Temajuk, Kecamatan Paloh memiliki keanekaragaman sumberdaya alam, termasuk makroalga. Lokasi pengambilan sampel sebanyak tiga stasiun dan ditentukan menggunakan metode purposive sampling, berdasarkan keberadaan makroalga. Sampling makroalga dilakukan menggunakan kuadrat transek dengan ukuran 10x10 m2 dan pengukuran parameter fisika-kimia perairan menggunakan WQC AZ 8603. Hasil penelitian menunjukkan bahwa jenis makroalga yang ditemukan yaitu Padina, Turbinaria, Sargassum, Acanthopora, dan Gracilaria. Dari semua jenis yang teridentifikasi, Phaeophyceae memiliki persen kontribusi lebih besar (60%), serta Padina dan Sargassum memiliki kelimpahan tertinggi, yaitu 21,31 ind/mek 2 dan 19,42 ind/m2 secara berturut-turut. Perairan Desa Temajuk memiliki tingkat keanekaragaman sedang, keseragaman tinggi, dan dominansi rendah. Kondisi perairan mempengaruhi kelimpahan makroalga di perairan Desa Temajuk, Kecamatan Paloh.   Macroalgae is one of the potential marine resources that ecologically plays a crucial role in increasing the water’s primary productivity, bioremediation of various types of pollutants, as food sources, providing oxygen and habitat for various aquatic biota. Economically, macroalgae also potential to be developed because it contains various bioactive compounds that can be used in various industrial fields. Species diversity and abundance of macroalgae are strongly influenced by physio-chemical water parameters. Temajuk Village located in Paloh District has a high diversity of natural resources, including macroalgae. The sampling locations were three stations and determined using purposive sampling method, based on the presence of macroalgae. A sampling of macroalgae was carried out using a quadratic transect with a size of 10x10 m2 and measurement of the physio-chemical parameters was done using WQC AZ 8603 instrument. The results showed that the genera of macroalgae found were Padina, Turbinaria, Sargassum, Acanthopora, and Gracilaria. Among the identified species, Phaeophyceae had the highest percentage contribution (60%), which Padina and Sargassum had the highest abundances, such as 21.31 ind/m2 and 19.42 ind/m2, respectively. The waters of Temajuk have a moderate level of diversity, high uniformity, and low dominance. Water conditions influenced the diversity and abundance of macroalgae in the water of Temajuk, Paloh District.
Pertumbuhan Benih Ikan Patin (Pangasius sp.) Pada Pemberian Pakan dengan Penambahan Asam Humat Tanah Gambut Azhanifa, Nur Atika Salma; Sari, Yuni Astika; Ardita, Weni; Yulianti, Eva; Maghfiroh, Wasiatul; Rousdy, Diah Wulandari
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.56629

Abstract

Patin fish (Pangasius sp.) is a superior commodity that is in demand and widely cultivated by the people of Indonesia. Feed is one of the things that has an impact in fish farming as a growth support. One way to increase feed efficiency is to use additional ingredients, in the form of peat soil humic substances. This study aims to determine the growth response and endurance of catfish seeds (Pangasius sp.) fed with additional humic acid. Feed was given as much as 2% of fish biomass using a completely randomized design (CRD) with five treatments, namely control and humic acid concentrations of 0.25%; 0.5%; 1%; and 2%, for 4 weeks. Each treatment was repeated three times with each replicate containing 10 catfish fry. The results showed that feeding humic acid supplementation with a humic acid concentration of 0.5% had a significant effect on the addition of the mean absolute weight of catfish to 2,394 ± 1,144 with the highest daily growth rate (0.080 ± 0.038 g/day).
The Therapeutic Potential of Humic Acid in Streptozotocin-Induced Hyperglycemic White Rats (Rattus norvegicus L.) Sirait, Evi Marina; Rousdy, Diah Wulandari; Yanti, Ari Hepi
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p110-117

Abstract

Humic acid, an organic compound from peat soil, rich in antioxidants such as quinone and phenol, shows potential as an antidiabetic agent. This study aims to identify the optimal dose of humic acid extract for increasing body weight, lowering blood glucose levels, reducing malondialdehyde (MDA) levels, and improving the microanatomical structure of the pancreas in streptozotocin-induced diabetic rats. The humic acid was extracted from peat soil at Tanjungpura University. The study utilized 25 male Wistar rats (Rattus norvegicus), aged 8-12 weeks, organized in a group randomized design (GRD). Interventions included a single dose of streptozotocin (40 mg/kg b.w.) and varying doses of humic acid extract administered over 14 days post-induction. Results indicated that a dose of 375 mg/kg b.w. humic acid extract resulted in an increased final body weight by 11.86%, a reduction in fasting blood glucose levels by 79.23%, an AUC0-120 value of 19.311, and a final MDA level of 1.09 ±0.27 nmol/ml. These findings indicate the potential of humic acid as a therapeutic agent for diabetes.
Co-Authors Afriza, Hanafi Anggraini, Wiwit Ardita, Weni Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Aswandi Aswandi, Aswandi Azhanifa, Nur Atika Salma Cesaria Anggun Kinanty Dwi Gusmalawati Edi Kurniadi Edi Kurniadi Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Eva Yulianti Filda Rahmawati Firman Saputra Firman Saputra Hanafi Afriza Harfan, Donatus Tia Hendro Priyandono Holyness Nurdin Singadimedja Ikha Safitri Irwan Lovadi Junardi Junardi Junardi Junardi Kesha Purwaning Sari Kustiati Kustiati Kustiati Kustiati Kustiati Maghfiroh, Wasiatul Marsianus Marthin Rivaldy Mega Sari Juane Sofiana Mukarlina -, Mukarlina Mukarlina Mukarlina Mukarlina, Mukarlina Nastiti Wijayanti Nora Idiawati Nurul Huda Prianti Prianti Prianti, Prianti Putri, Winda Eka Rafdinal Rafdinal Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati, Rahmawai Rahmawati, Rahmawati Rikhsan Kurniatuhadi, Rikhsan Riyandi Riyandi Riza Linda Rizki Perdana Putri Rusliandi Rusliandi Sangau, Paulus Sari, Yuni Astika Sirait, Evi Marina Siti Anikha Idzni Siti Ifadatin Turnip, Masnur Warsidah, Warsidah Wiwit Anggraini Yuniar, Helenda Ferni Asri Zulfa Zakiah