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All Journal Jurnal Bios Logos BULETIN OSEANOGRAFI MARINA Protobiont: Jurnal Elektronik Biologi SEMIRATA 2015 Majalah Obat Tradisional Jurnal Sains & Teknologi Lingkungan BIOMA Jurnal Bioteknologi & Biosains Indonesia (JBBI) JFMR (Journal of Fisheries and Marine Research) JURNAL PENGABDI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Jurnal Labora Medika Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Jurnal Entomologi Indonesia Jurnal Riset Biologi dan Aplikasinya Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Jurnal Borneo Akcaya : Jurnal Penelitian dan Pengembangan Pelayanan Publik Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian Perikanan Indonesia
Diah Wulandari Rousdy
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Tanjungpura, Pontianak, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Other

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INVENTARISASI MAKROINVERTEBRATA BENTIK DI SUNGAI MENTUKA KABUPATEN SEKADAU KALIMANTAN BARAT Sangau, Paulus; Junardi, Junardi; Rousdy, Diah Wulandari
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36848

Abstract

The existence of Ephemeroptera, Plecoptera and Trichoptera (EPT) macroinvertebrates in water can be used as the indicators to identify the water quality. This study aims to determine the diversity of Macroinvertebrates and physical and chemical factors in water of the Mentuka River. Macroinvertebrate sampling was carried out in February 2018 using random with surber and dip net at three stations spread overthe upstream, middle and downstream of the Mentuka River. The measurements chemicals and physical factors such as water temperature, brightness, current velocity, depth, TDS, TSS, acidity (pH), dissolved oxygen (DO) and free carbon dioxide (CO2), which is carried out together with macroinvertebrates sampling. There are 378 macroinvertebrates individuals belonging to nine genera, namely Polypedilum, Hydropsyche, Anthopotamus, Baetis, Acroneuria, Libellula, Rhyacophila, Parathelphusa and Macrobrachium in Mentuka River. The Ephemeroptera, Baetis is the most abundance with 5.76 ind/m2. Based on the diversity index, the Mentuka was categorized as moderately polluted river.
Skrining Fitokimia Berbagai Fraksi Ekstrak Buah Lakum (Cayratia trifolia L.) Elvi Rusmiyanto, Catur Wulandari, Diah Wulandari Rousdy,
Jurnal Protobiont Vol 7, No 2 (2018)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v7i2.26641

Abstract

Lakum fruit (Cayratia trifolia L.) is one of the plant which is predicted to have antioxidant activity. The aim of present study is to investigate the presence of phytoconstituents in the different fractions like ethyl acetate, methanol and n-hexane of lakum fruit extract. The fruit was extracted using methanol solvent. The result of fractionations showed that the ethyl acetate fraction have yield of 19,7%, while the methanol fraction have yield of 16,8% and the n-hexane fraction have yield of 2,26%. The ethyl acetate and methanol fractions were showing alkaloids and phenolic compounds, while n-hexane fraction only show alkaloids.
Profil Hematologi Pekerja Operator PT. PLN Sektor Kapuas PLTD Sungai Raya Pontianak Diah Wulandari Rousdy, Nisaa Madyan Fadilah, Ari Hepi Yanti,
Jurnal Protobiont Vol 5, No 2 (2016)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v5i2.15926

Abstract

Diesel Power Plant (PLTD) is an industry that uses diesel machines as electrical energy plant source. The machines which in PLTD create the sound intensity that exceeds the threshold value which can lead the workers to physiology stress. This research aims to determine the intensity of machines sound noise and hematological profile of PLTD operator workers differences that exposed to the sound of Sungai Raya PLTD machine based on their working period. This research was conducted from October to December2015. Samples which taken as many as 25 operator workers and 6 people carriers as control. The results showed that workers who exposed to machines sound while in the operator room of 80.84 dB and in the engine room of 105.24 dB. The intensity of diesel machines sound in engine room exceeds the threshold value of 85 dB that stipulated in the Decree of the Minister of Environment No. 48 of 1996. Operator workers with working period of 0-5 years has the number of leukocytes, LED level, the percentage ofneutrophils and monocytes higher compared to the control group and a longer working period. In contrast, the number of erythrocytes, hemoglobin levels and lymphocyte percentage of the operator workers 0-5 years lower than in the control group and the other working period. The percentages of hematocrit, eosinophils and basophils operator workers are no different to the control group and other working periods.Workers with longer working period have a body function that has been adapted to the working environment.
EFEKTIVITAS ANTIMIKROBA BUAH LAKUM (Cayratia trifolia [L.] DOMIN) TERHADAP PERTUMBUHAN BAKTERI Streptococcus sp. (L.10.3) Yuniar, Helenda Ferni Asri; Rahmawati, Rahmawati; Rousdy, Diah Wulandari
Jurnal Protobiont Vol 9, No 1 (2020)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v9i1.40719

Abstract

Streptococcus sp. (L.10.3) is one of the bacteria that can cause dental plaque. This study was aimed to determine the antimicrobial activity of the fraction of methanol fraction of fruit against Streptococcus sp. (L.10.3). This study used a Completely Randomized Design (CRD), 3 levels of concentrations of fraction methanol fraction 0.3 g/mL, 0.6 g/mL and 0.9 g/mL and 2 positive control treatments of amoxicillin and negative control using distilled water with three times repeated. Testing the antibacterial activity using the Kirby-Bauer method using disc paper. The results of the study showed the average diameter of inhibition zones of Streptococcus sp. (L.10.3) in the 24th hour incubation ranged from 7.70-10.72 mm and in the 48th hour incubation ranged from 8.70-10.37 mm. A concentration of 0.9 g/mL is an effective concentration with a moderate inhibition response category. Giving fraction of methanol fraction of fruit at the lowest concentration of 0.3 g/mL is able to provide a moderate inhibition response with the bacteriocidal antibacterial category.
Aktivitas Hepatoprotektif Ekstrak Metanol Buah Lakum (Cayratia trifolia (L.) Domin) Terhadap Diameter Vena Sentralis, Lebar Sinusoid dan Berat Hepar Tikus Putih (Rattus novergicus L.) yang Diinduksi Parasetamol Ari Hepi Yanti, Rizki Perdana Putri Diah Wulandari Rousdy,
Jurnal Protobiont Vol 7, No 3 (2018)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v7i3.29088

Abstract

Secondary metabolites of lakum (Cayratia trifolia (L.) Domin) fruit have been evaluated in previous researches. The fruit contains flavonoids, terpenoids and phenols which have antioxidant properties. The aim of this research was to determine the hepatoprotective activity of methanolic extract of lakum fruit in Wistar rats’ liver induced by high doses of paracetamol. Ripe lakum fruits were macerated with methanol as the solvent. Thirty-five male Wistar rats (Rattus novergicus L.) were used in this research and devided into 7 groups (normal, negative, positive, solvent, 115 mg kg-1, 230 mg kg-1, and 345 mg kg-1 of lakum fruit methanolic extract). Results showed that the effect of 230 mg kg-1 of lakum fruit methanolic extract (central vein diameter: 40,20 ± 6,97 µm; sinusoid wide: 5,60 ± 0,94 µm; and liver weight: 6,33 ± 0,98 g) were similar to the effect of silymarin (central vein diameter: 42,99 ± 6,60 µm; sinusoid wide: 4,49 ± 0,44 µm; and liver weight: 6,58 ± 0,88 g) as the standard drug.
Studi Analisis Perilaku Mencuci Tangan Terhadap Kepadatan Koloni Bakteri Sebelum dan Setelah Mencuci Tangan Pada Mahasiswa Diah Wulandari Rousdy, Dessy Kartika, Rahmawati,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.19494

Abstract

Handwashing with soap is a healthy behavior that has scientifically been proved to prevent the spread of infectious diseases. Students who are active on campus have risk factors of being contaminated by bacteria. This research aims to determine the correlation between the number of bacterial colonies on the palms and handwashing practices among students, and the difference between the number of bacterial colonies before and after washing hands with soap. This research used a pour plate method in accordance with the Standard Plate Count Method. The samples were 35 students. Based on the t-test, handwashing with soap showed a decrease in the number of bacterial colonies than before washing hands with soap, namely 1751.3 CFU / cm2 becoming 905.5 CFU / cm2 after washing hands. The logistic regression analysis and descriptive test showed that there is a correlation between the number of bacterial colonies on the palms and hand washing practices among students.
PENGARUH TUMBUHAN KABOMBA (Cabomba aquatica Aubl.) TERHADAP KADAR TSS, DO dan COD LIMBAH CAIR LABORATORIUM KIMIA FMIPA UNTAN Afriza, Hanafi; Mukarlina, Mukarlina; Rousdy, Diah Wulandari
Jurnal Protobiont Vol 8, No 3 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i3.36721

Abstract

Laboratory is one of the producers of waste water with a high content of hazardous materials before being discharged into water bodies. One effort to process laboratory waste with phytoremediation is to use Kabomba plants (Cabomba aquatica Aubl). This study aims to determine the ability of Kabomba (Cabomba aquatica Aubl.) To see the influence of plants on the levels of Total Suspended Solid (TSS), Dissolved Oksigen (DO) and Chemical Oxygen Demand (COD) from the Untreated Chemistry Laboratory Liquid Waste of FMIPA. This study used a Completely Randomized Design consisting of five treatments of waste concentration: control, 25, 50, 75, 100%. Based on the results of the study, Cabomba plants (Cabomba aquatica, Aubl.) able to reduce TSS values by 37.67% and increase DO values by a percentage of 77.5% on day 5 of treatment but not yet able to reduce COD values and increase pH values.
Kandungan Pigmen Klorofil, Karotenoid dan Antosianin Daun Caladium Diah Wulandari Rousdy, Hasidah, Mukarlina,
Jurnal Protobiont Vol 6, No 2 (2017)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v6i2.19711

Abstract

Caladium is a family Araceae which has various colors and patterns of leaves. The mechanism of variation of color and pattern on the leaves is caused by the chlorophyll pigment, carotenoid and anthocyanin. Each variation in color and pattern of the leaf has a different anatomical structure. This research aims to find out the differences in the content of the chlorophyll pigment, carotenoid and anthocyanin as well as examine the anatomical structure of the mesophyll in 6 Caladium plants. This research was conducted from January to May 2016. Measurement of chlorophyll, carotenoid and anthocyanin was conducted using a spectrophotometric method while mesophyll tissue anatomy using the method of wet incision preparation. The results showed that the highest chlorophyll content was found in the leaves of C. schomburgkii by 28.508 mg/l, while the highest carotenoid content was found in C. bicolor 1 by 35.780 μmol/g. The highest anthocyanin content was found in C. bicolor cultivar Blaze by 0.057 mg/g. The cross section area of the red, green and white showed a different mesophyll structure. The green area mesophyll tissue differentiates into palisade and spongy tissue containing chlorophyll, while the red and white mostly showed thet mesophyll tissue that did not differentiate into palisade and spongy tissue. The mesophyll cells in the red area contain anthocyanin, and the white area was not pigmented and there was space between the cells among the palisade tissues and between the upper epidermis and mesophyll tissue.
Peningkatan Kepedulian Masyarakat Kecamatan Pontianak Timur Terhadap Kesehatan Melalui Media Leaflet Rousdy, Diah Wulandari; Linda, Riza; Kustiati, Kustiati; Yanti, Ari Hepi; Rahmawati, Rahmawati; Riyandi, Riyandi; Junardi, Junardi; Ifadatin, Siti; Rusmiyanto, Elvi; Setyawati, Tri Rima; Kurniatuhadi, Rikhsan; Rafdinal, Rafdinal; Turnip, Masnur; Zakiah, Zulfa; Mukarlina, Mukarlina; Lovadi, Irwan; Saputra, Firman
Jurnal Pengabdi Vol 4, No 1 (2021): April 2021
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jplp2km.v4i1.44379

Abstract

Kesehatan merupakan faktor kebutuhan utama manusia untuk dapat melangsungkan aktivitas hidup dengan sempurna. Kesehatan masyarakat terganggu bila terdapat kuman yang menginfeksi tubuh. Corona  atau Severe  Acute  Respiratory  Syndrome Coronavirus  2 (SARS- CoV-2) adalah virus yang menyerang  sistem pernapasan. Penyakit ini dapat menyebabkan infeksi paru-paru yang berat, hingga kematian. Data bulan April 2020, Kecamatan Pontianak Timur dilaporkan mempunyai angka kasus positif Covid-19 paling tinggi diantara kecamatan lain di Kota Pontianak.  Kelurahan di Kecamatan Pontianak Timur yang dilaporkan mempunyai angka kasus Covid-19 antara lain Kelurahan Tanjung Hulu, Kelurahan Parit Mayor, Kelurahan Banjar Serasan, Kelurahan Tambelan Sampit, Kelurahan Dalam Bugis dan Kelurahan Tanjung Hilir. Kurangnya kesadaran masyarakat tentang kesehatan dan cara mengatasi permasalahan kesehatan sangat perlu dilakukan  suatu bentuk sosialisasi dengan cara memberikan edukasi tentang permasalahan kesehatan dan cara mengatasinya. Cara yang sangat memungkinkan pada masa pandemi ini adalah dengan tidak langsung bertatap muka namun dapat dilakukan secara tidak langsung yaitu melalui leaflet dan contoh produk.  Tujuan dari kegiatan ini adalah memberikan edukasi kesehatan dalam mencegah Covid-19 kepada masyarakat. Metode yang dilakukan dalam kegiatan ini adalah memberikan edukasi dan penyuluhan melalui leaflet yaitu cara pembuatan hand sanitizer dan masker serta contoh  produk hand sanitizer dan masker yang diberikan kepada masyarakat. Sebelum pelaksanaan kegiatan, sebagian besar masyarakat (90%) di Kecamatan Pontianak Timur belum menerapkan protokol pencegahan Covid-19 yang benar. Setelah pelaksanaan kegiatan diperoleh hasil berupa peningkatan kesadaran dan pengetahuan masyarakat (100%, n=100 orang) mengenai upaya pencegahan Covid-19 serta cara pembuatan masker dan hand sanitizer.
NILAI MALONDIALDEHID HEPAR MENCIT YANG DIINDUKSI PARASETAMOL PADA PEMBERIAN EKSTRAK METANOL KULIT KAYU Vitex pubescens Vahl Anggraini, Wiwit; Rousdy, Diah Wulandari; Wardoyo, Elvi Rusmiyanto Pancaning
Jurnal Protobiont Vol 10, No 1 (2021)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v10i1.50760

Abstract

Long-term consumption or overdose of paracetamol can induce liver damage characterized by increased measure of liver malondialdehyde (MDA). The methanol extract of laban tree bark (Vitex pubescens Vahl) contains antioxidant compounds such as flavonoids, tannins, and saponins that have potential as hepatoprotectors. This study aims to determine the optimal dose of methanol extract of V. pubescens tree bark to reduce liver MDA levels of mice after being induced by toxic dose of paracetamol (TDP). This study uses a completely randomized design (CRD) and 35 male Swiss strain mice aged 2,5-3 months. The treatments given consisted of normal control (aquades); negative control (TDP 105 mg/kg weight); positive control (vitamin E 48.6 mg/kg weight) and methanol extract of V. pubescens tree bark (75 mg/kg weight; 150 mg/Kg weight; 300 mg/kg weight and 600 mg/kg weight). Based on the research results, mice that were given an extract dose of 600 mg/kg weight showed a liver MDA levels of 0.68±0.24 nmol/mL which was close to the liver MDA levels of positive control mice, which was 0.62±0.12 nmol/mL.
Co-Authors Afriza, Hanafi Anggraini, Wiwit Ardita, Weni Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Aswandi Aswandi, Aswandi Azhanifa, Nur Atika Salma Cesaria Anggun Kinanty Dwi Gusmalawati Edi Kurniadi Edi Kurniadi Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Eva Yulianti Filda Rahmawati Firman Saputra Firman Saputra Hanafi Afriza Harfan, Donatus Tia Hendro Priyandono Holyness Nurdin Singadimedja Ikha Safitri Irwan Lovadi Junardi Junardi Junardi Junardi Kesha Purwaning Sari Kustiati Kustiati Kustiati Kustiati Kustiati Maghfiroh, Wasiatul Marsianus Marthin Rivaldy Mega Sari Juane Sofiana Mukarlina -, Mukarlina Mukarlina Mukarlina Mukarlina, Mukarlina Nastiti Wijayanti Nora Idiawati Nurul Huda Prianti Prianti Prianti, Prianti Putri, Winda Eka Rafdinal Rafdinal Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati, Rahmawai Rahmawati, Rahmawati Rikhsan Kurniatuhadi, Rikhsan Riyandi Riyandi Riza Linda Rizki Perdana Putri Rusliandi Rusliandi Sangau, Paulus Sari, Yuni Astika Sirait, Evi Marina Siti Anikha Idzni Siti Ifadatin Turnip, Masnur Warsidah, Warsidah Wiwit Anggraini Yuniar, Helenda Ferni Asri Zulfa Zakiah