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All Journal JBIO: jurnal biosains (the journal of biosciences) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Agricultural Science Indonesian Journal of Cancer Chemoprevention Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Biologi Tropis KACANEGARA Jurnal Pengabdian pada Masyarakat JURNAL PengaMAS Mattawang: Jurnal Pengabdian Masyarakat Seminar Nasional Pengabdian Kepada Masyarakat Anoa: Journal of Animal Husbandry Ininnawa: Jurnal Pengabdian Masyarakat Jurnal Hasil-Hasil Pengabdian dan Pemberdayaan Masyarakat (JHP2M) Jurnal Abdi Negeriku JKM: Jurnal Kemitraan Masyarakat
Kusdianawati
Universitas Negeri Makassar
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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CONSTRUCTION, EXPRESSION AND PURIFICATION OF RECOMBINANT PRE-MATURE PEPTIDE OF PLANTARICIN F FROM Lactobacillus plantarum S34 IN Escherichia coli Kusdianawati Kusdianawati; Apon Zaenal Mustopa; Suharsono Suharsono; Bugi Ratno Budiarto; Fatimah Fatimah; Hasim Danuri
Indonesian Journal of Agricultural Science Vol 16, No 1 (2015): April 2015
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v16n1.2015.p31-38

Abstract

Plantaricin is one of bacteriocins that have the potential to be used as food preservative. Plantaricin is safe for human consumption because it can be easily degraded by proteolytic enzymes. The objective of this study was to express and purify recombinant pre-mature peptide of plantaricin F from Lactobacillus plantarum S34 in Escherichia coli. Plantaricin gene-specific primer was used to obtain pln F structural gene amplicon from L. plantarum S34. This amplicon was cloned in pET32a vector and expressed in E. coli BL21 (DE3) pLysS. Pre-mature plantaricin F peptide was expressed as Histagged-fusion protein and separated by Co2+-chelating affinity chromatography. L. plantarum S34-derived pre-mature plantaricin F peptide fused with thioredoxin-(His)6tag had successfully been expressed in E. coli BL21 (DE3) pLysS using pET32a as an expression vector. The fused recombinant pln F as pre-mature state expressed had a molecular mass of +24 kDa, meanwhile the fused recombinant that contained only the leader peptide of pln F appeared as +20 kDa based on SDS-PAGE separations. The optimal production of fused recombinant pln F as soluble fraction was obtained when culture condition was added with 0.5 mM of IPTG and incubated at 22°C for 5 hours (OD~1). Furthermore, the expression of fused recombinant pln F as its pre-mature peptide pointed out that the pln F’s leader peptide could be proteolytically cleaved by a system in heterologous cells. Overall, heterologous pln F production as pre-mature peptide fused with thioredoxin-(His)6tag had been well established. From this research, we expect plantaricin F can be expressed and purified in E. coli.
Dangke Hasil Olahan dari Susu Kuda Liar Sumbawa, Susu Sapi Sumbawa, dan Susu Kerbau Sumbawa: Dangke from Sumbawa Horse Milk, Sumbawa Cow Milk, and Sumbawa Buffalo Milk Novi Fathiaturrahma; Kusdianawati; Lili Suharli
Anoa: Journal of Animal Husbandry Vol 1 No 2 (2022): Agustus
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/anoa.v1i2.31054

Abstract

Dangke is a local cheese product that is made enzymatically with the addition of papain enzyme through a heating process. Dangke is one of the innovations in processing food products (Sumbawa horse milk, Sumbawa cow's milk, and Sumbawa buffalo milk) which has the potential to increase the selling power of milk. The research method begins with each milk being heated over low heat until the temperature reaches 60 - 70˚C while stirring to ensure all parts of the milk get an even heat. Furthermore, the addition of papain enzyme as much as 0.1% v/v. After that, the milk will form curd and whey. Curd was taken using a spoon and then drained using a sieve and put into a coconut shell mold and allowed to stand for a few minutes until it becomes a dangke product. Then the dangke quality was observed by looking at several indicators. The results showed that the processing of Sumbawa horse milk, Sumbawa cow's milk, and Sumbawa buffalo milk succeeded in becoming dangke. Based on the indicators of dangke quality observation, the three types of milk were able to produce the same color and texture, namely milky white and soft texture. Meanwhile, for the indicators of aroma and length of clotting time, the three types of milk showed different results. Sumbawa wild horse milk requires ±9 minutes of clotting time, Sumbawa cow's milk for ±10 minutes, and Sumbawa buffalo milk for ±8 minutes.
Manufacturing Natural Woven Dyes From Mangrove In Dusun Prajak, Desa Batu Bangka, Sumbawa: Pembuatan Pewarna Alami Tenun Dari Mangrove Di Dusun Prajak, Desa Batu Bangka Sumbawa Lili Suharli; Izzul Islam; Riri Rimbun Anggih Chaidir; Kusdianawati Kusdianawati
Mattawang: Jurnal Pengabdian Masyarakat Vol. 3 No. 4 (2022)
Publisher : Yayasan Ahmar Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (888.637 KB) | DOI: 10.35877/454RI.mattawang978

Abstract

Department of the environment around 10.660 hectares of mangroves with varuous types. One that has the potential for mangrove development is Dusun Prajak, located in the Saleh bay area which is directly adjacent to the Moyo Tambora biosphere zone that has a major influence on the preservation of marine natural resources such as coral and whale sharks. The purpose is to provide skill in making natural dyes made from mangroves to community. The method is observation, interviews, practice and assistance with the Participatory Rural Appraisal (PRA) approach. Furtermore, mentoring and evaluation are carried out. The survey result show that weaving based on natural dyes from mangroves has never been done, so it is important to carry out demonstrations of making mangrove dyes. The training program received high appreciation from the community because it was uniqe and hope that program would continue and community would be assisted. Abstrak Berdasarkan data Dinas Lingkungan Hidup, sekitar 10.660 hektar mangrove dengan jenis yang beragam. Salah satu yang memiliki potensi pengembangan mangrove adalah Dusun Prajak, terletak di wilayah Teluk Saleh yang langsung berbatasan dengan zona biosfer Moyo-Tambora sehingga dusun prajak berpengaruh besar dalam kelestarian SDA laut seperti karang dan seperti Hiu Paus. Tujuan pengabdian yaitu memberikan skill pembuatan pewarna tenun alami berbahan mangrove kepada masyarakat. Metode yang digunakan adalah metode observasi, wawancara, praktik dan pendampingan dengan pendekatan Participatory Rural Appraisal. Selanjutnya dilakukan pendampingan dan evaluasi. Hasil survei menunjukkan bahwa tenun berbasis bahan pewarna alami dari mangrove belum pernah dilakukan, sehingga penting dilaksanakan demonstrasi pembuatan pewarna mangrove. Program pengabdian ini mendapat apresiasi tinggi dan berharap program ini terus dilanjutkan dan masyarakat didampingi.
METABOLITE ACTIVITY OF LACTIC ACID BACTERIA FROM SUMBAWA HORSE MILK, WEST NUSA TENGGARA, INDONESIA Kusdianawati Kusdianawati; Apon Zaenal Mustopa; Fatimah Fatimah; Nurlaili Ekawati
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 1 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i1.37701

Abstract

Sumbawa horse milk is one of the local products in Sumbawa (West Nusa Tenggara). There is no research so far which discusses the potencies of LAB metabolite compounds as antimicrobials and probiotics for treating Diabetes Mellitus type 2. This study aims to obtain the antimicrobial and inhibitory activity of the LAB-extracted metabolite compounds from Sumbawa horse milk collected from Penyaring village and Lennanguar village, Sumbawa, West Nusa Tenggara, Indonesia. LAB metabolites are extracted by using 96% ethanol. It has been found that metabolites extracted from media culture of LAB SKP K.9 (E. thailandicus) have the highest antimicrobial activity against S. aureus (ATCC 6538) (2.5 cm), S. typhimurium (2.5 cm), L. monocytogenes (BTCC B693) (2.53 cm) and EPEC K.1.1 (2.7 cm) when compared with other LAB isolates. Moreover, the inhibition of α-glucosidase enzyme obtained from LAB SKP K.5 isolate (L. garvieae) is 6.25%, 39.28% from LAB SKP K.9 (E. thailandicus), 53.57% from LAB SKP K.3 (W. confusa), 67.85% from LAB SKL K.4 (L. fermentum), and 78.57% from LAB SK 1.5 (E. faecium). The extract of LAB SK 1.5 metabolite compound (E. faecium) has the highest inhibitory activity against the α-glucosidase enzyme (78.57%) compared to other LAB isolates.
Isolasi Bakteri Asam Laktat dari Susu Kuda Liar dan Potensi Antibakteri pada Susu Kuda Liar Sumbawa Baso Manguntung; Abdhi Surya Perkasa; Yulianti Kusdianawati; Heru Piria Hastuti; Arif Muhamad
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 3, No 2 (2018): June 2018
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v3i2.1894

Abstract

Pulau Sumbawa yang terletak tepat di provinsi Nusa Tenggara Barat menjadi salah satu pulau yang menghasilkan Susu Kuda Liar Sumbawa terbesar di Indonesia. Susu kuda asal Sumbawa sangat diminati karena khasiatnya yang sangat baik untuk kesehatan. Tujuan dilakukannya penelitian ini adalah untuk mengisolasi bakteri asam laktat dari susu kuda liar Sumbawa dan mengetahui potensi kemampuan antibakteri dari susu kuda liar Sumbawa. Penelitian ini dilakukan dengan mengisolasi bakteri asam laktat dari susu kuda liar Sumbawa dengan metode spread plate menggunakan media MRSA. Kemudian uji antibakteri susu kuda liar Sumbawa dilakukan dengan metode difusi paper disk terhadap tiga spesies bakteri patogen berbeda yaitu Eschericia coli, Staphylococcus aureus, Vibrio cholerae dan perlakuan kontrol (-) akuades, kontrol (+) kanamisin. Pengujian menggunakan susu kuda liar Sumbawa dengan variasi konsentrasi berbeda yaitu 20, 40, 60, 80, dan 100. Hasil penelitian menunjukkan terdapat bakteri asam laktat pada susu kuda liar Sumbawa dengan tumbuhnya koloni berwarna putih pada media MRSA. Pada pengujian antibakteri terlihat adanya zona hambat pada semua perlakuan konsentrasi dan kontrol (+). Diameter zona hambat terbesar dilihat pada konsentrasi 100% yaitu Staphylococcus aureus (1,996 mm), Eschericia coli (1,982 mm), dan Vibrio cholerae (2,020 mm) sedangkan kontrol (-) tidak menunjukkan adanya zona hambat. Sehingga susu kuda liar Sumbawa ini berpotensi menghambat terhadap bakteri patogen.
Pengembangan Ekonomi Lokal : Pelatihan Pengembangan Tenun Motif Hiu Paus di Desa Poto, Kabupaten Sumbawa, Provinsi Nusa Tenggara Barat Kusdianawati Kusdianawati; Izzul Islam; Lili Suharli; Riri Rimbun Anggih Chaidir
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Publisher : UNIVERSITAS KHAIRUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/pengamas.v6i1.4973

Abstract

Industri kerajinan tenun Sumbawa di Dusun Samri, Desa Poto merupakan salah satu usaha yang diwariskan turun-temurun secara tradisional yang masih berupa kegiatan sampingan dalam pemenuhan kebutuhan sehari-hari. Namun kini industri kerajinan tenun tersebut sedang dalam proses pengembangan untuk menjadi sentra industri kecil untuk hasil tenun Sumbawa dan dapat digunakan untuk memenuhi kebutuhan masyarakat serta sebagai media promosi kearifan budaya lokal. Oleh karena itu, untuk membantu dalam proses pengembangan tenun Sumbawa khususnya yang ada di Dusun Samri, Desa Poto maka diadakannya kegiatan pengabdian masyarakat berupa pengembangan tenun motif hiu paus oleh Fakultas Teknobiologi, Universitas Teknologi Sumbawa. Metode pelaksanaan kegiatan meliputi persiapan dan pelatihan. Pelatihan dilakukan dengan pemaparan materi motif hiu paus yang digunakan pada kain tenun Sumbawa. Motif hiu paus yang digunakan dibuat dulu desainnya di kertas kotak kemudian diterapkan oleh partisipan dalam pelatihan. Setelah itu, dilanjutkan dengan pembuatan motif hiu paus pada kain tenun Sumbawa yang dilakukan oleh peserta pelatihan secara langsung. Kegiatan pengabdian ini menghasilkan kain tenun Sumbawa dengan motif hiu paus dan ada juga yang dipadukan dengan motif lama yang sering digunakan dalam kain tenun Sumbawa. Hasil kegiatan pengabdian berupa tenun Sumbawa dengan motif hiu paus dapat dihasilkan dan diproduksi oleh masyarakat di Dusun Samri, Desa Poto.
Pemanfaatan potensi lokal Desa Mokong Sumbawa NTB melalui pelatihan pembuatan Virgin Coconut Oil (VCO) Chaidir, Riri Rimbun Anggih; Suharli, Lili; Kusdianawati, Kusdianawati; Islam, Izzul
KACANEGARA Jurnal Pengabdian pada Masyarakat Vol 6, No 3 (2023): Agustus
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/kacanegara.v6i3.1594

Abstract

Desa Mokong berada di Kabupaten Sumbawa, Nusa Tenggara Barat dan mata pencarian utama masyarakatnya adalah bertani. Masyarakat Desa Mokong biasa menanam dan memanen kelapa atau membeli dan menjual buahnya begitu saja, tanpa mengetahui pemanfaatannya sebagai minyak nabati khususnya Virgin Coconut Oil (VCO). Secara umum, VCO banyak dikonsumsi untuk tujuan kesehatan dan kecantikan. Produksi VCO dapat membantu meningkatkan nilai kelapa di Desa Mokong dan dapat menjadi produk unggulan Desa Mokong. Kegiatan pengabdian ini adalah pelatihan pembuatan VCO dengan metode kombinasi. Metode ini sederhana, sehingga pembuatan VCO ini dapat dilaksanakan kembali oleh peserta di rumah masing-masing. Pelaksanaan kegiatan pengabdian dilakukan dalam beberapa tahap, yaitu persiapan, pembuatan VCO dan pelaksanaan kegiatan pengabdian. Pelaksanaan kegiatan pengabdian pula terdiri dari sesi pemberian kuisioner sebelum dan sesudah pelatihan, pemberian materi dan diskusi serta praktek. Hasil evaluasi kegiatan melalui kuisioner menunjukkan adanya peningkatan pengetahuan mengenai VCO, ketertarikan untuk mengonsumsi VCO dan untuk mengikuti pelatihan ini dan selanjutnya.
Respon Siswa Madrasah Aliyah Terhadap Pelatihan Pembuatan Kefir Nani Kurnia; Andi Mu'nisa; Kusdianawati; Muhammad Junda
Jurnal Hasil-Hasil Pengabdian dan Pemberdayaan Masyarakat Vol. 3 No. 2 (2024): Volume 03 Nomor 02 (Oktober 2024)
Publisher : Jurusan Matematika FMIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/jhp2m.v3i2.5369

Abstract

Kefir is an average result of fermented milk using kefir grains as a starter that contains lactic acid bacteria and yeast. Kefir drinks are believed to affect the balance of intestinal microbes positively. In developing countries, kefir is produced on a factory scale and is marketed internationally by various brands. This differs in Indonesia; kefir is still produced on a small scale and sold in limited quantities and in restricted areas. Considering the benefits and market potential, the public must know about the benefits and how to make kefir. Therefore, knowledge transfer activities and training in making kefir to the community are very important to be carried out, one of which is to school students. The school students who can be selected are high. Therefore, this activity aims to provide training for MA DDI Mattoangin, Bantaeng Regency students training in making kefir. The implementation method used in this activity consists of 4 stages: presentation, demonstration, practice, and evaluation. This activity has been carried out at MI DDI Mattoangin, Bantaeng Regency, and was attended by 11 participants. The participants enthusiastically followed all the activities, which ended with following and paying attention to the presentation, the kefir-making demonstration, practicing kefir-making, and filling out the questionnaire
Pengembangan Ekonomi Lokal : Pelatihan Pembuatan Gantungan Kunci Imajinatif Hiu Paus di Desa Labuhan Jambu Kusdianawati, Kusdianawati; Islam, Izzul; Suharli, Lili; Chaidir, Riri Rimbun Anggih
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Publisher : LPPM UNIVERSITAS KHAIRUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/pengamas.v7i2.8381

Abstract

Pengembangan ekonomi lokal merupakan langkah strategis untuk meningkatkan kesejahteraan masyarakat melalui pemanfaatan potensi daerah. Desa Labuhan Jambu termasuk salah satu desa yang mengembangkan ekonomi lokal memalui ekowisata hiu paus. Desa ini memiliki potensi dalam pengembangan kerajinan tangan berbasis kreativitas berupa pembuatan gantungan kunci imajinatif berbentuk hiu paus. Pelatihan ini bertujuan untuk memberdayakan masyarakat desa terutama ibu-ibu rumah tangga dan pemuda setempat agar memiliki keterampilan sekaligus memberikan edukasi tentang potensi wisata hiu paus yang berkelanjutan. Pelatihan ini menggunakan metode praktik langsung dimana masyarakat didampingi oleh tim PkM dalam pembuatan gantungan kunci imajinatif hiu paus. Ada dua tahap yang dilakukan pada proses pembuatan gantungan kunci yaitu tahap pengecatan dan tahap pelapisan. Selain itu, pelatihan juga mencakup teknik dasar pencampuran resin dan fiksasi warna sehingga bisa menjadi gantungan kunci yang menarik dan bernilai jual tinggi. Hasil dari pelatihan menunjukkan peningkatan keterampilan dan pengetahuan peserta dalam bidang kerajinan tangan dalam menghasilkan gantungan kunci. Produk gantungan kunci hiu paus diharapkan menjadi daya tarik tersendiri untuk menarik minat pasar lokal maupun wisatawan yang berkunjung ke desa Labuhan Jambu. Harapannya pelatihan ini tidak hanya memberikan manfaat ekonomi tetapi juga membangun rasa percaya diri dan kebanggaan di kalangan peserta untuk bisa terus menjaga wisata hiu paus yang berkelanjutan. Kata Kunci: Gantungan Kunci Hiu Paus, Labuhan Jambu, Ekonomi Lokal, Edukasi
Pelatihan Pembuatan Minuman Probiotik Tepache Siswa MA Muhammadiyah Cambajawaya Kabupaten Gowa Jumadi, Oslan; Kurnia, Nani; Kusdianawati, Kusdianawati; Azis, Andi Asmawati; Junda, Muhammad
JKM: Jurnal Kemitraan Masyarakat Volume 4 Number 1 Juni 2025
Publisher : Program Studi Pendidikan IPA Fakultas MIPA Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/jkm.v4i1.74282

Abstract

A training session on making the probiotic drink tepache was conducted at MA Muhammadiyah Cambajawaya, Gowa Regency, as an educational initiative to promote health and utilize local resources. Tepache is a traditional fermented beverage made from pineapple peel and pulp, rich in probiotics beneficial for digestive health. This activity aimed to enhance students’ understanding of probiotics, train practical skills in producing environmentally friendly fermented drinks, and foster entrepreneurial spirit. The training method included theoretical sessions, live demonstrations, and hands-on practice. Evaluation results showed that the majority of students (87%) expressed interest in making tepache independently and understood its health benefits. The training also introduced the concept of productive use of household organic waste. In conclusion, this activity effectively educated students and opened opportunities for developing local health-based products. Similar training programs are recommended to be expanded with greater institutional and governmental support.
Co-Authors Abd Muis Abdhi Surya Perkasa Andi Asmawati Azis Andi Faridah Arsal Andi Mu'nisa Apon Zaenal Mustopa Apon Zaenal Mustopa Apon Zaenal Mustopa Arif Muhamad Arsal, Andi St. Fahirah Arsal, Andi St. Fatmah Baso Manguntung Besse Khalidatunnisa Bugi Ratno Budiarto Chaidir, Riri Rimbun Anggih Fatimah Fatimah Fatimah Fatimah Hartati Hartati Hartono Hartono Hasim Danuri Heru Piria Hastuti Isma, Auliana Bestray Ismail izzul islam Lestari, Novi Cahya Lodang, Hamka Muhammad Junda Musawira, Musawira Nani Kurnia, Nani Novi Fathiaturrahma Nurlaili Ekawati Oslan Jumadi Patongai, Dian Dwi Putri Ulan Sari Popi Hadi Wisnuwardhani, Popi Hadi Pratiwi, Andi Citra Ramly, Zakia Asrifah RATIH ASMANA NINGRUM Saiful Anwar Shidiq, Fajrin Suharli, Lili Suharsono, Suharsono Susiati, Asih Luklu Syamsiah, Syamsiah Vanggi, Leggina Rezzy