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PERAN SISTEMATIKA MIKROBIA DALAM MENGUNGKAP KEANEKARAGAMAN MIKROORGANISME Idramsa, Idramsa
JURNAL KELUARGA SEHAT SEJAHTERA Vol 11 No 2 (2013): JURNAL KELUARGA SEHAT SEJAHTERA
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jkss.v11i22.3564

Abstract

ABSTRACTDiversity of bacteria and archaea  can not be learned completely   yet not identified perfectly. This is due to the abundant amount of diversity of microorganisms. The existence of molecular technologies allow to identify quickly and accurately the organism. Classification approach is based on the value of character and phylogenetic fenetik make a meaningful classification of microorganisms. Microbes such as bacteria systematic approach should be based on systematic polyfasik. Kata Kunci : Klasifikasi, fenetik, filogenetik, sistematika mikrobia
DESKRIPSI JAMUR ENDOFIT DARI TUMBUHAN RARU (Cotylelobium melanoxylon) GENUS BOTRYTIS Hasanah, Uswatun; Idramsa, Idramsa
JURNAL KELUARGA SEHAT SEJAHTERA Vol 15 No 1 (2017): JURNAL KELUARGA SEHAT SEJAHTERA
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jkss.v15i29.7171

Abstract

ABSTRACK This study aims to identify the endophytic fungus from the bark of the plant raru (Cotylelobium melanoxylon). This study uses identification technique manually by viewing and comparing the macroscopic and microscopic morphological characteristics of each isolate endophytic fungi then seek to obtain dendogram similarity index which divides each isolate into several clusters/genus. Data were analyzed using cluster analysis Simple Matching Coefficient in the Multi Variate Statistical Package (MVSP) where each isolate that had ≥70 % similarity indices are grouped into one cluster/genus. The results of cluster analysis to get 8 cluster/genus endophytic fungi found in the bark of plants raru (Cotylelobium melanoxylon). Eighth genus included the genus Alternaria, Aspergillus, Botrytis, Debaromyces, Fusarium, Mycelia sterile, Nigrospora, and Scopulariopsis. This paper presents a description of the genus Botrytis. Kata Kunci: Identifikasi, Jamur Endofit, Cotylelobium Malanoxylon,  Indeks Similiritas
In vitro plant regeneration of Cattleya sp. from Protocorm-Like Bodies (PLBs) using coconut water and activated charcoal HARAHAP, FAUZIYAH; SIREGAR, ANITA RASUNA SARI; IDRAMSA, IDRAMSA; SURIANI, CICIK; EDI, SYAHMI; PANGGABEAN, NURUL HUDA; DAULAE, ABDUL HAKIM; POHAN, SELVIA DEWI; TANJUNG, AURA ALBIZIA; PERTIWI, SUFINA INTAN; KAIRANI, ANITA
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100100

Abstract

Abstract. Harahap F, Siregar ARS, Idramsa, Suriani C, Edi S, Panggabean NH, Daulae AH, Pohan SD, Tanjung AA, Pertiwi SI, Kairani A. 2026. In vitro plant regeneration of Cattleya sp. from Protocorm-Like Bodies (PLBs) using coconut water and activated charcoal. Asian J Agric 10 (1): g100130. https://doi.org/10.13057/asianjagric/g100130. Cattleya sp. is an ornamental orchid whose seeds lack of endosperm, making natural germination and propagation difficult. In vitro culture using Protocorm-Like Bodies (PLBs) offers an alternative for mass propagation. This study aimed to evaluate the effects of coconut water and activated charcoal on the in vitro regeneration of Cattleya sp. PLBs. A completely randomized design was employed with varying concentrations of coconut water (0, 5, and 10%) and activated charcoal (0, 0.1, 0.2, and 0.3 g/L). Morphological parameters observed included time to shoot emergence, number of leaves, shoots, and roots, as well as shoot color. Data were analyzed using two-way ANOVA. The results showed that coconut water significantly accelerated shoot emergence with 10% of coconut water producing the fastest response (12 days after treatment). Activated charcoal enhanced shoot and leaf formation with 0.3 g/L, producing the highest number of shoot and leaf. Root formation was optimal at lower charcoal concentrations. Overall, the combination of coconut water and activated charcoal significantly influenced PLBS growth and regeneration. These findings indicate that appropriate combinations of organic additives can optimize in vitro regeneration of Cattleya sp. and support efficient orchid propagation.