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All Journal Agrointek REKAYASA PENA SAINS QUANTUM: Jurnal Inovasi Pendidikan Sains Unnes Science Education Journal Kumawula: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Mathematics and Natural Science Education Natural Science Education Research (NSER) Prosiding Seminar Nasional Hasil-hasil Penelitian dan Pengabdian Pada Masyarakat Indonesian Journal of Mathematics and Natural Science Education
Laila Khamsatul Muharrami, Laila Khamsatul
Universitas Trunojoyo Madura
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Mathematics Physics Public Health Social Sciences Other

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Journal : Agrointek

 1 
PENENTUAN KADAR KOLESTEROL DENGAN METODE KROMATOGRAFI GAS Laila Khamsatul Muharrami
AGROINTEK Vol 5, No 1 (2011)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v5i1.1932

Abstract

The methods for quantitative determination of cholesterol in foods by chromatography determination Two types of lipid classes including free cholesterol and cholesterol ester exist in food. Total cholesterol is measured after chemical hydrolysis of cholesterol esters to free cholesterolPrior to instrumental analysis, cholesterol is extracted from the food sample and saponified. The amount of the food sample depends on the cholesterol content; a sample containing ∼1 mg of cholesterol is required for lipid extraction. Saponification hydrolyzes the carboxyl group attached to carbon in cholesterol and yields only free cholesterol in unsaponifiable matter. The unsaponifiable matter can be extracted by n-hexane, diethyl ether, or petroleum ether. In the present protocol, unsaponifiable matter is extracted using n-hexane. This protocol requires prior knowledge of GC  methodologies and adherence to instructions from the instrument’s manufacturer.
Co-Authors Afifah, ST Ahied, Moch Ahmad Ali Aida Fikriyah Aida Fikriyah Aida Fikriyah Aini, Kamilatul Aini, Wardatul Aldiono, M. Amanda, Sutria Anggraeni, Dyan Novi Arova, R Balkis Awaliyah, Nur Irmiyanni A’yun, Ulfi Qurrotu Catur Wasonowati Devi, Silvia Sandrias Dian Sri Utami Famila, Shanti Dwi Fatimatul Munawaroh Fatimatul Munawaroh Fatimatul Munawaroh Irsad Rosidi, Irsad Islamiyah, Mega Isroila, Afida Kameliyah Kameliyah Khasanah, Eka Kristanti Nur krisnawati, dwi rahayu Laili Cahyani Laili, Nuri Alfiyatul Mardi Santoso Maryamah, Siti Mellyani, Ety Mochammad Ahied Mochammad Ahied MOCHAMMAD YASIR Mochammad Yasir Muallafah, Rini Munawarah, Fatimatul Munawwaroh, Fatimatul Murdaningrum, Rika Mursyidah, Rossy Mustamiroh, Rizqiyatul Nafsah, Wilda Zakyatun Nazilah, Nurun Nur Qomaria Nur Qomaria Paraswati, Kolikah Putera, Dwi Bagus Rendy Astid Qomaria, Nur Qomariya, Yuyun Rianita, Tias Rizki Indriana Efendi Rusdianti, Anna Khoyriyah Sari, Dewi Putri Puspita Sholikah, Niswatin Siti Zulaikah Subaidah, Tus Taslim Ersam wilujeng, ayu Wiwin Puspita Hadi Wulandari, Ana Yuniasti Retno Yunin Hidayati yunin hidayati