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All Journal BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Widyadari : Jurnal Pendidikan
Anak Agung Istri Mirah Dharmadewi
Universitas PGRI Mahadewa Indonesia
Biochemistry, Genetics & Molecular Biology Education

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PENGARUH PEMBELAJARAN CONTEXTUAL TEACHING AND LEARNING BERBASIS LINGKUNGAN SEKOLAH TERHADAP KREATIVITAS DAN HASIL BELAJAR BIOLOGI PESERTA DIDIK KELAS XI SMA NEGERI 9 DENPASAR I Made Subrata; A. A. Istri Mirah Dharmadewi; Herlina Lium
Widyadari Vol. 26 No. 2 (2025): Oktober 2025
Publisher : LP3M Universitas PGRI Mahadewa Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59672/widyadari.v26i2.5569

Abstract

This study aims to determine the effect of the school environment-based Contextual Teaching and Learning (CTL) learning model on the creativity and biology learning outcomes of class XI students of SMA Negeri 9 Denpasar in the 2024/2025 academic year. The CTL model emphasizes the connection between subject matter and real life, which in this study is based on the school environment. The population of this study was all 11th grade students of SMA Negeri 9, and two classes were sampled, each with 36 students. One class served as the experimental group and the other as the control group. The experimental group was given environment-based CTL learning, while the control group was given conventional learning with material presentation and questions and answers. Data were collected through learning outcome tests and creativity sheets. The research data were analyzed using t-test and MANOVA statistical analysis. Prior to the parametric statistical analysis, prerequisite tests were conducted in the form of normality, validity, and homogeneity tests. The results of the statistical analysis showed that the average learning creativity of the experimental class was 87.75 and learning outcomes were 84.94. Meanwhile, the control class obtained average scores of 78.36 and 72.97, respectively. The MANOVA test showed a significance value of 0.000 (<0.05) and Wilk's Lambda of 0.158, indicating that there was a simultaneous influence of the school-based Contextual Teaching and Learning (CTL) learning model on students' creativity and biology learning outcomes. These results prove that the school-based CTL model is effective in improving the quality of biology learning at SMA Negeri 9 Denpasar in the 2024/2025 academic year.
LC-MS/MS Based Identification of Antifungal Bioactive Compounds from White Turi (Sesbania grandiflora L.) Stem Bark Extract Mirah, A. A. Istri; Darmadi, A. A. K.; Suriani, N. L.; Suanda, I.W.; Subrata, I.M.
Biotik Vol 14 No 1 (2026): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v14i1.34455

Abstract

White turi (Sesbania grandiflora L.) is a tropical plant belonging to the Fabaceae family that is widely distributed and known for its diverse pharmacological activities. However, the utilization of its stem bark as a source of antifungal agents has been relatively underexplored. This study aimed to identify the phytochemical profile and bioactive compounds of white turi stem bark extract with potential antifungal properties. The sample was extracted using a maceration method with 70% ethanol for 72 hours. Qualitative phytochemical screening was conducted using standard methods, including Mayer, Wagner, Dragendorff, Liebermann–Burchard, and FeCl₃ assays. Further analysis was performed using LC-MS/MS (Agilent 6545 Q-TOF LC/MS System) to identify bioactive compounds. The results revealed the presence of alkaloids, terpenoids, phenolics, tannins, and saponins. LC-MS/MS analysis of the F6 water fraction identified several potential antifungal compounds, including adenine, phenylethyl alcohol, benzenedicarboxylic acid bis (2-ethylhexyl) ester, octadecane, and 2,3-dimethoxybenzaldehyde. These compounds are known to exhibit antifungal mechanisms through disruption of cell membranes, protein denaturation, and inhibition of fungal cellular metabolism. Overall, the findings suggest that white turi stem bark extract has strong potential for development as a natural antifungal agent and as a raw material for plant-based health products. Keyword: Bioactive compounds; natural health products; Sesbania grandiflora L.; LC-MS/MS (Agilent 6545 Q-TOF LC/MS System).
Co-Authors Darmadi, A. A. K. Herlina Lium I Made Subrata N. L. Suriani, N. L. Suanda, I.W. Subrata, I.M.