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Potensi produk komersial berbahan aktif Beauveria bassiana sebagai agensia pengendalian Oryctes rhinoceros Ardika, Made; Fitriana, Yuyun; Suharjo, Radix; Purnomo, Purnomo
Jurnal Proteksi Agrikultura Vol. 1 No. 1 (2024): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1128-33

Abstract

The rhinoceros beetle, Oryctes rhinoceros, is a major pest on coconut and oil palm plants, attacking both in replanting areas and directly in farmers' fields. The O. rhinoceros beetle (Coleoptera: Scarabaeidae) holds the most important position as a pest of coconut plants, both in terms of its damaging ability, distribution, population stability, and year-round population size, and is widely recognized by coconut farmers. The use of microorganisms as biological agents is part of biological control efforts. One biological agent widely used as a bioinsecticide and proven to be quite effective is the entomopathogenic fungi group, such as Beauveria bassiana. The objectives of this research are as follows: 1). To determine the ability of a commercial product containing B. bassiana as an active ingredient to cause mortality in O. rhinoceros larvae; 2) To identify the effective dose that can cause high mortality in O. rhinoceros. This study used a Completely Randomized Design (CRD) with five treatments tested, namely: P1: 37.5 g B. bassiana; P2: 28.13 g B. bassiana; P3: 18.75 g B. bassiana; P4: 9.38 g B. bassiana; P5: control. Each treatment was repeated five times, and each replicate consisted of five samples, resulting in 25 experimental samples or a total of 125 O. rhinoceros larvae. Further analysis was conducted using the LSD test at a 5% significance level. Based on the research results, the commercial product containing B. bassiana as an active ingredient was able to cause mortality in O. rhinoceros. The most effective dose for controlling O. rhinoceros larvae was treatment 3, with a dose of 18.75 g, yielding an average mortality rate of 72%.
Eksplorasi dan identifikasi bakteri simbion wereng batang coklat (Nilaparvata lugens Stal.) sebagai pendegradasi insektisida imidakloprid Sanjaya, Muhamad Sony; Fitriana, Yuyun; Lestari, Puji; Suharjo, Radix
Jurnal Proteksi Agrikultura Vol. 1 No. 1 (2024): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1120-27

Abstract

Rice is an important food crop and a staple food source in Indonesia. The challenges in increasing rice production are not only caused by weather factors (drought, flooding) but also by pest and plant disease attacks. One of the rice pests that causes significant losses is the brown planthopper (BPH). Insecticides with the active ingredient imidacloprid have been known to effectively control brown planthoppers. However, in recent times, resistance to this insecticide has developed in brown planthoppers. This study aims to investigate the possible presence of symbiotic bacteria in brown planthoppers that can degrade imidacloprid insecticide as their carbon source. The steps taken include biochemical tests and testing the growth ability on media containing imidacloprid. The results of the tests showed that the bacterial isolates tested were unable to degrade the imidacloprid insecticide.
Spodoptera frugiperda: Hama utama pada tanaman jagung dan strategi pengendaliannya Anintyas, Dytri; Safitri, Khofifah Nur Indah; Kusumawati, Eka; Masoyogie, Kholfira; Dirmawati, Suskandini Ratih; Aeny, Titik Nur; Suharjo, Radix
Jurnal Proteksi Agrikultura Vol. 1 No. 2 (2024): NOVEMBER, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.2166-80

Abstract

Spodoptera frugiperda (FAW), or fall armyworm, is a major invasive pest that attacks corn crops in various tropical and subtropical regions. In recent decades, the global spread of FAW has been accelerated by international trade and climate change. In Indonesia, the presence of this pest was first reported in 2019 in West Sumatra, with attacks spreading to various other areas. The FAW life cycle includes four stages (eggs, larvae, pupae, and imago) with significant levels of plant damage, especially in the larval stage.Various con- trol strategies have been implemented, including integrated pest management (IPM) approaches, such as the use of biological agents (Beauveria bassiana and Metarhizium anisopliae), push-pull techniques, and crop rotation. In addition, the development of pest-resistant corn varieties is an important alternative. Recent re- search has identified two genetic lines of FAW, namely corn and rice lines, which show differences in host preference and resistance to insecticides.This study highlights the need for further development of ecologi- cally based strategies, including exploration of local parasitoids and effective bioinsecticide formulations. In addition, genetic and microbiota analyses offer new potential in understanding FAW population dynamics and its control. These results are expected to be the basis for sustainable pest management in Indonesia and other regions.
PELATIHAN PEMBUATAN PESTISIDA NABATI BERBASIS PEMBERDAYAAN PETANI DI KELOMPOK TANI MEKAR SARI, LAMPUNG SELATAN Fitriana, Yuyun; Suharjo, Radix; Efri, Efri; Susilo, FX; Lestari, Puji; Helina, Selvi
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 8, No 6 (2025): MARTABE : JURNAL PENGABDIAN KEPADA MASYARAKAT
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v8i6.2436-2443

Abstract

Kelompok Tani Mekar Sari di Desa Hajimena, Kecamatan Natar, Kabupaten Lampung Selatan, menghadapi tantangan berupa serangan hama dan penyakit tanaman yang berdampak pada penurunan hasil panen. Tingginya ketergantungan terhadap pestisida kimia memotivasi diadakannya pelatihan pembuatan pestisida nabati sebagai alternatif pengendalian yang ramah lingkungan. Kegiatan pengabdian ini bertujuan untuk mengenalkan jenis tanaman yang dapat digunakan sebagai pestisida nabati dan melatih petani dalam membuat serta mengaplikasikannya. Metode yang digunakan mencakup penyuluhan dan praktik langsung. Pada sesi pertama, materi yang disampaikan meliputi konsep dasar, manfaat, dan langkah pembuatan pestisida nabati. Pada sesi kedua, dilakukan praktik pembuatan pestisida nabati berbahan dasar daun nimba (Azadirachta indica), yang melibatkan 23 petani sebagai partisipan. Hasil pengabdian menunjukkan peningkatan pengetahuan petani terkait bahaya pestisida kimia, penggunaan pestisida nabati, serta cara pengendalian hama dan penyakit. Berdasarkan evaluasi, persentase pemahaman petani tentang pestisida nabati meningkat sebesar 56,52%. Kegiatan ini tidak hanya memberikan solusi praktis bagi petani, tetapi juga menjadi langkah awal menuju praktik pertanian yang lebih berkelanjutan dan ramah lingkungan.
PENGARUH APLIKASI Trichoderma sp DAN PUPUK MAJEMUK UNTUK MENEKAN PENYAKIT HAWAR DAUN DAN MENINGKATKAN PRODUKSI TANAMAN MELON (Cucumis melo L) Ratih, Suskandini; Hendarto, Kus; Suharjo, Radix; Admaja, Gede Kusuma
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.9853

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh Trichoderma sp. dan pupuk majemuk terhadap penyakit hawar daun dan produksi tanaman melon (Cucumis melo L). Penelitian ini dilaksanakan dilaksanakan di laboratorium Lapang Terpadu Fakultas Pertanian, Universitas Lampung, Provinsi Lampung. Penelitian ini dilaksanakan pada bulan Mei sampai dengan bulan Agustus 2021. Rancangan yang digunakan dalam percobaan ini adalah Rancangan Acak Kelompok (RAK) yang disusun secara faktorial. Faktor pertama adalah Trichoderma sp. yang terdiri dari 2 level yaitu tanpa Trichoderma sp (T0) dan dengan menggunakan Trichoderma sp. 20 g (T1). Faktor kedua dengan menggunkan pupuk majemuk yang terdiri dari 0 g (T0), 30 g (T1), 60 g (T2), dan 90 g (T3). Variabel yang diamati adalah keterjadian penyakit, keparahan penyakit, panjang tanaman, jumlah bunga betina, jumlah bungan jantan, diameter buah melon, dan bobot buah melon. Data pengamatan yang diperoleh dianalisis menggunakan sidik ragam dan selanjutnya diuji dengan uji BNT pada taraf 5%. Hasil penelitian bahwa Trichoderma sp. 20 g dapat menekan keparahan penyakit dan dapat meningkatkan bobot buah melon. Tidak ada interaksi antara perlakuan Trichoderma sp. 20 g dan pupuk majemuk terhadap panjang tanaman, keterjadian penyakit, jumlah bunga betina, jumlah bunga jantan dan diameter buah melon. Perlakuan pupuk majemuk 90 g dapat meningkatkan jumlah bunga betina dan meningkatkan bobot buah tanaman melon. Kemudian pada perlakuan pupuk majemuk 30 g dapat meningkatkan bobot buah tanaman melon. Terdapat interaksi antara perlakuan Trichoderma sp. 20 g dan pupuk majemuk 0 g yang dapat menekan keparahan penyakit tanaman melon. Kemudian Terdapat interaksi antara perlakuan Trichoderma sp. 20 g dan pupuk majemuk 90 g yang mampu meningkatkan bobot buah tanaman melon.
Endophytic Beauveria bassiana in root, seed, and foliar-treated maize affecting on Spodoptera frugiperda larvae and its parasitization eggs Herlinda, Siti; Saripudin, Saripudin; Thosin, Thosin; Madya, Sakha Prawira; Sari, Jelly Milinia Puspita; Suwandi, Suwandi; Anggraini, Erise; Suharjo, Radix
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 1 (2026): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12659-67

Abstract

Spodoptera frugiperda is a highly polyphagous pest capable of causing up to 100% yield loss in maize. This study aimed to evaluate the effects of endophytic Beauveria bassiana applied through root drench, seed treatment, and foliar spray on the severity of larval feeding damage and egg parasitization by S. frugiperda. The B. bassiana isolate (JgSPK) used in this study was molecularly identified and deposited in GenBank (acc. no. MZ356494). Results indicated that among the three inoculation methods, seed treatment resulted in the highest endophytic colonization, followed by soil drench and foliar spray. Feeding on leaves colonized by the fungus increased larval mortality and resulted in abnormal pupation. Seed treatment showed the greatest potential in reducing larval feeding severity compared with foliar spray and soil drench applications. However, none of the inoculation methods significantly affected egg parasitization rates. Overall, endophytic B. bassiana reduced larval attack severity but did not influence egg parasitization, indicating compatibility with egg parasitoids. Further research is recommended to evaluate potential effects on larval parasitoids.
Co-Authors Adhy ARIYANTO Adi Setiawan Admaja, Gede Kusuma Agus Muhammad Hariri, Agus Muhammad Ainin Niswati, Ainin Ali, Mufty AM, Shakila Larasati Anak Agung Gede Sugianthara Anggraini, Desma Anintyas, Dytri Ardika, Made Aristoteles, Aristoteles Aulia, Shifa Veronica Basuki, Muh. Cipta Ginting, Cipta Dad Resiworo Sembodo, Dad Resiworo David IRVANTO Dermiyati, Dermiyati Dewi, Ni Kadek Emi Sintha Efri, Efri Eka Kusumawati, Eka Erise Anggraini, Erise F.X. Susilo, F.X. Fakhrurreza, Moch Fakhmi Fiandani, Ambar Franciscus Xaverius Susilo, Franciscus Xaverius FX Susilo, FX Hartaman, Maman Hasriadi Mat Akin Helina, Selvi I GEDE SWIBAWA, I GEDE Ika Arthalia Wulandari Indriyati . Jayanti, Watini Hefri Joko Prasetyo Joko Prasetyo Kurniawan, Adam Fajar Kus Hendarto, Kus Lestari Wibowo Madya, Sakha Prawira Maria Viva Rini Marlinawati, Fransiska Dina Masoyogie, Kholfira Masyuda, Imam Maya Gustina Merdiana, Eryka Muhammad Nurdin Muhammad Nurdin Muhammad Nurdin Mu’arif, Imam Al Nabilah Nabilah Nova Adelina Lubis Pangaribuan, Darwin Hasibaran PUJI LESTARI Purnomo Purnomo Rani, Icha Deska Ratdiana, Ratdiana Rosma Hasibuan, Rosma Rudianto Butarbutar Ruruh Anjar Rwandini, Ruruh Anjar Safitri, Khofifah Nur Indah Sanjaya, Muhamad Sony Sari, Jelly Milinia Puspita Sari, Ni Nengah Yulyani Purnami Saripudin Saripudin Selviani, Zakiah Setyo Dwi Utomo SITI HERLINDA Solikhin ., Solikhin Solikhin Solikhin Suputa Suputa Suskandini Ratih Dirmawati Suwandi Suwandi Taha, Farhan Thosin, Thosin Titik Nur Aeny Titik Nur Aeny Tri Maryono Wahyudi Wahyudi Wardiyani, Wardiyani Wati, Tri Agus Setiya Yuyun Fitriana Zahra, Fatimah Az