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All Journal Journal of Tropical Soils JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Komputasi Jurnal Agrotek Tropika Martabe : Jurnal Pengabdian Kepada Masyarakat Menara Perkebunan Agrica: Journal of Sustainable Dryland Agriculture Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Jurnal Proteksi Agrikultura
Radix Suharjo
Jurusan Agroteknologi, Fakultas Pertanian Universitas Lampung
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Environmental Science Social Sciences

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PENGARUH APLIKASI BIONEMATISIDA PURPUREOCILLIUM LILACINUM TERHADAP KOMUNITAS NEMATODA PADA LAHAN JAMBU KRISTAL Dewi, Ni Kadek Emi Sintha; Zahra, Fatimah Az; Swibawa, I Gede; Suharjo, Radix; Fitriana, Yuyun; Maryono, Tri; Lestari, Puji; Helina, Selvi; Aulia, Shifa Veronica; Wardiyani, Wardiyani; Marlinawati, Fransiska Dina; Basuki, Muh.; Ratdiana, Ratdiana
AGRICA Vol. 18 No. 1 (2025): June
Publisher : Agriculture Faculty of Flores University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/agr.v18i1.5728

Abstract

Crystal guava (Psidium guajava var. crystal) is a high-value horticultural commodity, but its production in several regions has declined due to infestations by plant-parasitic nematodes. This study aimed to evaluate the effects of a bionematicide formulated with Purpureocillium lilacinum on the composition and diversity of soil nematode communities in crystal guava cultivation. Assessments were conducted on nematode abundance, absolute frequency, prominence value, and diversity metrics, including the Shannon index, evenness, dominance, and species richness, before and after the application of compost and bionematicide treatments. The results revealed the presence of 11 nematode genera, with Aphelenchus being the most dominant. Both compost and bionematicide treatments led to a decline in most genera, except Aphelenchoides, which increased in both abundance and prominence, and Rhabditis, which showed an increase in abundance but a reduction in ecological value. Post-treatment measurements indicated decreases in diversity, evenness, and richness indices, alongside an increase in dominance, particularly following compost application. These findings suggest that while P. Lilacinum is effective in targeting certain nematode groups; however, it may also reduce overall nematode community diversity, potentially leading to the dominance of the Aphelenchus genus. Thus, its application should be accompanied by ecological impact assessments to ensure the long-term sustainability of soil ecosystems.
Selection of four types arbuscular mycorrhizal fungi in oil palm seedling planted in histosol soil Maria Viva RINI; Radix SUHARJO; Lestari WIBOWO; David IRVANTO; Adhy ARIYANTO
Menara Perkebunan Vol. 89 No. 1 (2021): 89 (1), 2021
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v89i1.406

Abstract

Arbuscular mycorrhizal fungi (AMF) are beneficial microbes for plants, especially in the absorption of nutrients and water from the soil. Some factors that influence the effectiveness of this fungus are the suitability of the AMF species with their host plants and abiotic condition such as soil characteristics. Therefore, this study aims to determine the type of AMF that produced the best growth and nutrient uptake in oil palm seedlings planting in histosol soil. This study used a single factor treatment design consisting of 9 AMF treatments, namely control without AMF (T1), given Glomus sp. (T2), Gigaspora sp. (T3), Entrophospora sp. (T4), Acaulospora sp. (T5), a mixture of Glomus sp. and Gigaspora sp. (T6), a mixture of Glomus sp. and Entrophospora sp. (T7), a mixture of Gigaspora sp. and Entrophospora sp. (T8), and a mixture of Glomus sp., Gigaspora sp., Entrophospora sp., and Acaulospora sp. (T9). Each treatment was repeated five times. The experimental design used was a randomized block design. In the AMF treatment, 500 spores per seedling were given when the seedlings were transplanted from prenursery to the main nursery. The seedlings were kept in the prenursery for 3.5 months and in the main nursery for nine months. The results showed that the type of mycorrhizal that consistently produced better seedling growth and nutrients uptake compared to the control were Glomus sp. (T2) and a mixture of Gigaspora sp. and Entrophospora sp. (T8), which supported by data on plant height, number of leaves, shoot dry weight, and total nutrient uptake of N, P, K, Ca, Mg, and B.
Pengendalian Dan Perbanyakan Agensia Pengendali Hayati Hama Dan Penyakit Tanaman Padi Di Desa Karya Mulya Sari Candipuro Lampung Selatan Suharjo, Radix; Fitriana, Yuyun; Lestari, Puji; Helina, Selvi
Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol. 3 No. 1 (2024): Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 3 No 1, Maret 2024
Publisher : Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpfp.v3i1.8694

Abstract

Tanaman padi merupakan tanaman pangan utama yang banyak dibudidayakan petani. Namun, tanaman padi sering mengalami banyak permasalahan seperti serangan hama dan penyakit. Oleh karena itu, agar produksi padi dapat meningkat, perlu adanya tindakan khusus untuk mengendalikan hama dan penyakit salah satunya dengan menggunakan agensia pengendali hayati (APH). APH yang banyak dilaporkan mampu mengendalian hama dan penyakit adalah jamur Metarhizium, Beauveria dan Trichoderma. Ketiga jamur tersebut dirasa perlu dikenalkan kepada warga Desa Karya Mulya Sari Candipuro Lampung Selatan, agar tanaman padinya dapat memberikan hasil yang optimal. Tujuan dari pelatihan ini adalah 1) meningkatkan pengetahuan petani tentang jenis-jenis hama dan penyakit yang sering menyerang tanaman padi, 2) meningkatkan keterampilan petani dalam mengelola dan mengendalikan hama dan penyakit tanaman padi khususnya menggunakan agensia pengendali hayati (APH) serta 3) melatih petani untuk dapat membuat perbanyakan agensia pengendali hayati jamur untuk mengendalikan hama dan penyakit tanaman padi. Kegiatan pelatihan dilaksanakan pada tanggal 4 Maret 2023 yang dihadiri sebanyak 16 orang. Pada penyuluhan ini Tim penyuluh melakukan ceramah tentang pengendalian hama dan dan penyakit menggunakan APH berupa jamur Metarhizium, Beauveria dan Trichoderma dan praktek perbanyakan APH dalam media beras. Dari petani yang mengikuti telah kegiatan ini, selanjutnya diharapkan dapat menyebarluaskan pengetahuan mereka kepada petani lain di desa atau kelompok tani lain sehingga sasaran dari kegiatan ini akan semakin meluas.
SOSIALISASI PENGGUNAAN PERANGKAP BERFEROMON UNTUK MENGENDALIKAN SERANGAN HAMA INVASIF SPODOPTERA FRUGIPERDA DI LAMPUNG TIMUR Lestari, Puji; Wibowo, Lestari; Maryono, Tri; Suharjo, Radix; Fitriana, Yuyun
Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol. 3 No. 2 (2024): Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Vol 3 No 2, September
Publisher : Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpfp.v3i2.9710

Abstract

Jagung adalah komoditas unggulan Kabupaten Lampung Timur. Invasi hama Spodoptera frugiperda menjadi masalah serius dalam budidaya jagung. Sejak awal masuknya hama S. frugiperda pengendalian yang dilakukan oleh petani di Kecamatan Batanghari Nuban adalah dengan mengunakan insektisida berbahan aktif emamectin benzoat. Namun penggunaan bahan aktif yang sama dalam waktu yang cukup lama dapat mempercepat terjadinya resistensi. Berdasarkan laporan dari Penyuluh Pertanian Lapangan (PPL), beberapa desa di Kecamatan Batanghari Nuban telah mengalami resistensi emamectin benzoat. Berdasarkan hal tersebut dirasa sangat perlu dilakukan penyuluhan terkait pengendalian S. frugiperda yang efektif ramah lingkungan. Tujuan dari kegiatan ini adalah untuk memberikan solusi dalam pengendalian hama invasif S. frugiperda yang telah mengalami resistensi terhadap bahan aktif emamectin benzoate dengan menggunakan perangkap berferomon. Perangkap berferomon ini dapat mengendalikan populasi S. frugiperda dengan cara yang ramah lingkungan sehingga mengurangi kerusakan pada tanaman jagung dan juga mengurangi dampak negatif penggunaan insektisida. Dengan demikian dapat mengurangi kerugian secara ekonomi dan mendukung sistem pertanian yang berkelanjutan. Hasil dari kegiatan ini petani dapat menggunakan feromon untuk memerangkap imago jantan S. frugiperda, sehingga dapat menurunkan populasi hama pada pertanaman jagung. Dengan menggunakan petangkap berferomon, petani dapat mengurangi biaya pengendalian dengan insektisida. Selain itu, dampak negatif dari insektisida juga dapat dikurangi.
Soil Organisms Activities in Red Onion Cultivation with Application of Plant Extract Suspension and Compost Rani, Icha Deska; Dermiyati, Dermiyati; Suharjo, Radix; Niswati, Ainin; Pangaribuan, Darwin Hasibaran
JOURNAL OF TROPICAL SOILS Vol. 27 No. 2: May 2022
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2022.v27i2.89-98

Abstract

Agro-industrial waste such as banana weevil, pineapple rhizome, and empty fruit bunches of oil palm has not been utilized properly, even though the waste can be processed into liquid organic fertilizer after extracting the beneficial microorganisms contained in it. This research aimed to observe and study soil organisms’ activity after applying plant extract suspensions and compost types. The research was conducted at the Integrated Field Laboratory and Soil Science Laboratory, Faculty of Agriculture, University of Lampung. The treatments were designed in a 3 × 3 factorial design in a Randomized Block Design with three replications. The first factor was the plant suspension extracts derived from banana weevil, pineapple rhizome, and oil palm empty fruit bunches, while the second factor was the compost in the form of solid compost and liquid compost. The results showed the activity of soil organisms in the form of respiration, soil microbial carbon biomass, soil microbial population, and mesofauna population, which was applied to a suspension of plant extracts from pineapple rhizome, given the highest yield. Furthermore, the bacterial phosphate dissolution index and the diversity of soil mesofauna applied to liquid compost were higher than those of solid compost or without compost. There was no interaction between the application of plant extract suspension and the compost types on the soil organism’s activity.
Kajian taksonomi penyakit bulai pada tanaman jagung dan pengendaliannya wahyudi, wahyudi; Selviani, Zakiah; Wati, Tri Agus Setiya; Sari, Ni Nengah Yulyani Purnami; Jayanti, Watini Hefri; Dirmawati, Suskandini Ratih; Aeny, Titik Nur; Suharjo, Radix
Jurnal Proteksi Agrikultura Vol. 2 No. 1 (2025): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1213-21

Abstract

Downy mildew disease in maize plants (Zea mays) caused by the pathogen Peronosclerospora maydis is a serious threat to maize production in Indonesia, which is one of the main food commodities. The disease can cause significant crop yield losses, so a deep understanding of the taxonomy, epidemiology, and control strategies of the disease is important. This study aims to identify the taxonomy of downy mildew, analyze environmental factors that affect the progression of the disease, and evaluate various effective control methods, both chemically and biologically. Downy mildew control is carried out through an integrated approach which includes the use of resistant corn variations, the application of crop rotation systems, environmental sanitation, and seed treatment with metalaxic fungicides. In addition, the use of biocontrol agents such as Trichoderma spp. and Pseudomonas fluorescens showed great potential in reducing infections by up to 75% and significantly improving crop yields. This study also highlights the importance of the development and application of plant-based fungicides derived from natural ingredients such as turmeric, garlic, and soursop leaves, which have antimicrobial properties. Although the use of biocontrol agents has been implemented in some areas, the adoption of this technology is still limited, so more research is needed to explore the potential of other biocontrol agents. These findings are expected to contribute to the development of sustainable downy mildew control strategies, increase corn productivity, and support national food security.
Begomovirus: Potensi ancaman pertanian hortikultura Lampung Helina, Selvi; Akin, Hasriadi Mat; Dirmawati, Suskandini Ratih; Fitriana, Yuyun; Maryono, Tri; Suharjo, Radix; Ginting, Cipta
Jurnal Proteksi Agrikultura Vol. 2 No. 2 (2025): NOVEMBER, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.2242-55

Abstract

Begomoviruses are a group of plant viruses belonging to the Geminiviridae family, persistently transmitted by the whitefly Bemisia tabaci, and pose a serious threat to horticultural crops in Lampung Province, Indonesia. These viruses infection symptoms such as mosaic, chlorosis, leaf curling, and stunted growth, with yield losses reaching up to 90%. The spread is exacerbated by intensive cultivation practices, the use of uncertified seeds, and tropical climates that favor high vector populations. This study explores the biological characteristics of Begomoviruses, their transmission mechanisms via vectors, and their high genetic variability due to frequent mutation and recombination. Several begomovirus species have been identified infecting key horticultural crops in Lampung, including Ageratum yellow vein virus (AYVV), Tomato yellow leaf curl Kanchanaburi virus (TYLCKaV), and Pepper yellow leaf curl Indonesia virus (PepYLCIV). In addition to vector transmission, some begomovirus species are also known to be seed-transmitted, expanding their epidemic potential. Integrated management strategies discussed in this article include the use of resistant varieties, biological and agronomic control of vectors, and the development of innovative approaches such as mild strain cross-protection. This review emphasizes the need for continuous monitoring and science-based sustainable strategies to mitigate the impact of Begomoviruses on food security and horticultural productivity in tropical regions such as Lampung.
Karakterisasi penyebab penyakit kanker batang pada pepaya (Carica papayae L.) di Kecamatan Limau, Kabupaten Tanggamus, Lampung AM, Shakila Larasati; Suharjo, Radix; Maryono, Tri; Masyuda, Imam; Taha, Farhan
Jurnal Proteksi Agrikultura Vol. 1 No. 1 (2024): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.111-13

Abstract

This study aims to determine the pathogen characteristics of papaya stem cancer and identify alternative host plants other than papaya. The research was conducted from October 2020 to May 2021 in the Laboratory of Agricultural Biotechnology and the Laboratory of Plant Diseases, Faculty of Agriculture, University of Lampung. Four bacterial isolates suspected to be the cause of papaya stem cancer in Limau (Tang 2(1), Tang 2(2), Ptl 1(2), Ptl 2(2)) were used in this study. Bacterial characterization was determined based on the results of biochemical tests. The results showed that the bacterial isolates were gram-negative, fermentative, lecithinase-negative, soft rot-negative, and hypersensitivity-negative, and did not produce fluorescence on King's B media. All isolates were positive for the arginine dihydrolase test, except for Tang 2(2) and Ptl 1(2). All isolates showed a negative reaction in the casein test, except for Tang 2(2). All isolates were able to grow at 39 °C or 40 °C. All bacterial isolates were able to utilize mannitol, L-tartrate, and mannitol, but not ascorbic acid. The results of the host range test showed that the bacteria were able to infect and cause symptoms in eggplant, chickpeas, luffa, and long beans. A pathogenicity test was conducted on 2-month-old papaya seedlings (cv. Calina). The results showed that all four bacterial isolates from Limau caused stem cancer symptoms in the papaya seedlings.
Kemampuan produk bioinsektisida komersial berbahan aktif Metarhizium sp. dalam menyebabkan kematian larva Oryctes rhinoceros L. Fakhrurreza, Moch Fakhmi; Fitriana, Yuyun; Suharjo, Radix; Swibawa, I Gede
Jurnal Proteksi Agrikultura Vol. 1 No. 1 (2024): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1114-19

Abstract

The rhinoceros beetle (Oryctes rhinoceros L.) (Coleoptera: Scarabaeidae) is a significant pest reported to attack nearly all coconut and oil palm plantations in Indonesia. Metarhizium sp., a well-known biological control agent, has broad-spectrum insecticidal properties due to its production of destruxin toxins, which are particularly toxic to O. rhinoceros. However, the effectiveness of dry-formulation bioinsecticides containing Metarhizium for controlling this pest has not yet been fully assessed. Therefore, this study was conducted to evaluate the potential of a dry-formulated Metarhizium sp. bioinsecticide to induce mortality in O. rhinoceros larvae. The research objectives were: 1) to assess the ability of the dry formulation bioinsecticide with Metarhizium as the active ingredient to induce mortality in O. rhinoceros larvae; 2) to determine the most effective dose of the dry formulation bioinsecticide with Metarhizium sp. for causing high mortality rates in O. rhinoceros. The study employed a Completely Randomized Design (CRD) with five treatments: 37.5 g (P1), 28.13 g (P2), 18.75 g (P3), and 9.38 g (P4) of Metarhizium sp., along with a control (P5). Each experimental unit consisted of a 1000 ml jar containing 500 g of coconut stem powder and five O. rhinoceros larvae, replicated five times with a total of 125 larvae. Analysis was conducted using the LSD test at a 5% significance level. Results indicated that the bioinsecticide was effective, with the most effective dose being 18.75 g, which resulted in 76.00% mortality.
Pengaruh modifikasi media S terhadap produksi spora, viabilitas, dan patogenisitas jamur agensia hayati Mu’arif, Imam Al; Fitriana, Yuyun; Suharjo, Radix; Swibawa, I Gede
Jurnal Proteksi Agrikultura Vol. 1 No. 1 (2024): MAY, JURNAL PROTEKSI AGRIKULTURA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpa.1134-45

Abstract

Biological control fungi can be stored and produced in the form of spores on a specific medium. For mass production, a large quantity of spores is required in a short amount of time. This study aims to determine the effect of medium S on the sporulation, spore viability, and pathogenicity of the fungi Beauveria bassiana, Metarhizium flavoviride, Purpureocillium lilacinum, and Trichoderma asperellum. There were two sets of experiments in this study. The first experiment tested the growth ability of fungal isolates on modified medium S in vitro. The second experiment tested the viability of the fungi that had been grown on the medium used in the previous experiment. The results showed that the type of fungus and the type of medium affected growth and spore production but did not influence spore viability. Among the biological agents, there appears to be mutual inhibition, though this inhibition does not seem to affect their ability to cause the death of the test insects. The consortium of biological agents resulted in a higher mortality rate of the test insects compared to single-agent applications.
Co-Authors Adhy ARIYANTO Adi Setiawan Admaja, Gede Kusuma Agus Muhammad Hariri, Agus Muhammad Ainin Niswati, Ainin Ali, Mufty AM, Shakila Larasati Anak Agung Gede Sugianthara Anggraini, Desma Anintyas, Dytri Ardika, Made Aristoteles, Aristoteles Aulia, Shifa Veronica Basuki, Muh. Cipta Ginting, Cipta Dad Resiworo Sembodo, Dad Resiworo David IRVANTO Dermiyati, Dermiyati Dewi, Ni Kadek Emi Sintha Efri, Efri Eka Kusumawati, Eka Erise Anggraini, Erise F.X. Susilo, F.X. Fakhrurreza, Moch Fakhmi Fiandani, Ambar Franciscus Xaverius Susilo, Franciscus Xaverius FX Susilo, FX Hartaman, Maman Hasriadi Mat Akin Helina, Selvi I GEDE SWIBAWA, I GEDE Ika Arthalia Wulandari Indriyati . Jayanti, Watini Hefri Joko Prasetyo Joko Prasetyo Kurniawan, Adam Fajar Kus Hendarto, Kus Lestari Wibowo Madya, Sakha Prawira Maria Viva Rini Marlinawati, Fransiska Dina Masoyogie, Kholfira Masyuda, Imam Maya Gustina Merdiana, Eryka Muhammad Nurdin Muhammad Nurdin Muhammad Nurdin Mu’arif, Imam Al Nabilah Nabilah Nova Adelina Lubis Pangaribuan, Darwin Hasibaran PUJI LESTARI Purnomo Purnomo Rani, Icha Deska Ratdiana, Ratdiana Rosma Hasibuan, Rosma Rudianto Butarbutar Ruruh Anjar Rwandini, Ruruh Anjar Safitri, Khofifah Nur Indah Sanjaya, Muhamad Sony Sari, Jelly Milinia Puspita Sari, Ni Nengah Yulyani Purnami Saripudin Saripudin Selviani, Zakiah Setyo Dwi Utomo SITI HERLINDA Solikhin ., Solikhin Solikhin Solikhin Suputa Suputa Suskandini Ratih Dirmawati Suwandi Suwandi Taha, Farhan Thosin, Thosin Titik Nur Aeny Titik Nur Aeny Tri Maryono Wahyudi Wahyudi Wardiyani, Wardiyani Wati, Tri Agus Setiya Yuyun Fitriana Zahra, Fatimah Az