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All Journal Health Science Journal of Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Indonesian Journal of Urology The Indonesian Biomedical Journal Padjadjaran Journal of Dentistry Molecular and Cellular Biomedical Sciences (MCBS) INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Indonesian Journal of Cancer Indonesian Journal of Biomedicine and Clinical Sciences
Didik Setyo Heriyanto
Department Of Anatomical Pathology, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada/Dr. Sardjito General Hospital, Yogyakarta
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences

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Increasing quantitative estrogen receptor beta expression in meningioma after exogenous hormonal contraception exposure Sakti, Dhimas Hari; Sasongko, Muhammad Bayu; Pawiroranu, Suhardjo; Heriyanto, Didik Setyo; Mahayana, Indra Tri; Respatika, Datu; Widhasari, Idhayu Anggit; Nurlaila, Prima Sugesty; Supartoto, Agus
Indonesian Journal of Biomedicine and Clinical Sciences Vol 57 No 4 (2025)
Publisher : Published by Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/inajbcs.v57i4.19604

Abstract

Factors influencing estrogen receptor (ER) and progesterone receptor (PR) expression in meningioma are unclear. This study aimed to assess the expression of ER and PR in females with meningioma in relation to patient exogenous hormonal exposure. Cross-sectional study of females with meningioma in Yogyakarta, Indonesia, between 2010 to 2016 was conducted. Histories were obtained through interviews. Expressions of ERa, ERb, and PR were assessed from paraffin blocks containing patients’ meningioma tissue using real-time quantitative polymerase chain reaction (RT-qPCR). The study identified 28 female meningioma patients with a mean age of 45.6 ± 6.8 yr. Increased ERb expression was found in the ³10-yr group of hormonal contraception exposure duration (<10 yr vs ³10 yr = 7.63 (2.3-11.31) vs 12.56 (1.87-42.22), p=0.038). Positive correlation was found between ERb expression and the duration of hormonal contraception exposure (r=0.432; p=0.022), especially progesterone-only contraception (r=0.5; p=0.048), while no significant correlation was found between ERb expression and duration of progesterone-estrogen contraception exposure (r=0.382; p=0.22). No significant analyses were found in ERa and PR. In conclusion, expression of ERβ in meningioma increased in accordance with exogenous hormonal exposure duration, especially progesterone. This finding suggests the importance of ERβ in meningioma and warrants further study as a potential biomarker.
MiR-141-3p Relative Expression Level from FFPE Samples as Biomarker of Prostate Adenocarcinoma Carcinogenesis in Yogyakarta, Indonesia Sari Eka Pratiwi; Sri Nuryani Wahyuningrum; Rachmagreta Perdana Putri; Danarto Danarto; Didik Setyo Heriyanto; Nur Arfian; Sofia Mubarika Haryana; Indwiani Astuti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 1 (2022): The future of diagnostic laboratory testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i1.2355

Abstract

Globally, prostate cancer (PCA) is the second leading cause of male cancer-associated mortality. Micro-RNAs (miRNAs) are small non-coding RNAs considered promising biomarkers for diagnosis, prognosis, and treatment options. A miR-141 expression is frequently dysregulated and influences the development and progression of PCA. This study aimed to identify miR-141 expression level as a marker to differentiate PCA from another prostate anomaly, especially in Yogyakarta. Formalin-fixed paraffin-embedded (FFPE) tissues for each three groups: benign prostatic hyperplasia/BPH, high-grade prostatic intraepithelial neoplasia/HGPIN, and PCA (n=7/group) were stored in a commercial clinical laboratory in Yogyakarta. The total RNA was extracted from FFPE sections using miRNeasy FFPE kit, followed by the quantification of miR-141-3p expression level by RT-PCR. The result showed that miR-141 relative expression level on PCA was higher than other groups and significantly different (P<0.05, Kruskal Wallis test). The mean of the miR-141 relative expression level of BPH, HGPIN, and PCA were 1.04±0.87, 6.44±7.8, and 7.06±8.83, respectively. The relative expression level of miR-141 can potentially be a prognostic biomarker in PCA and could differentiate aggressiveness in prostate anomaly, especially BPH, HGPIN, and PCA.
Efficiency and challenges of exosome and microRNA extraction from clinical biofluids in Indonesia: A commercial kit-based approach for qPCR applications Aprilia Indra Kartika; Muchamad Dafip; Nastiti Wijayanti; Didik Setyo Heriyanto; Sofia Mubarika Haryana; Kartika Widayati Taroeno-Hariadi
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 8 No 2 (2026): In Progress
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v8i2.7350

Abstract

Exosome miRNA has significant potential as biomarker for minimal or non-invasive diagnosis and monitoring of disease progression. However, extraction of miRNA exosomes from biofluids is challenging, and there is a lack of comparative evaluation of commercially available extraction kits for qPCR applications. Therefore, this study aims to obtain information about miRNA exosome extraction using commercial kits and analyze its expression with the qRT-PCR method. The exosome extraction was carried out using 3 types of commercial kits, while the RNA extraction was performed with a spin column. The purity and concentration of exosomes were measured using NTA, while total RNA was measured with nanodrops. Subsequently, miRNA expression was measured using qRT-PCR. The results showed that the concentration of exosomes obtained using kit 1 for plasma isolation was 3.5 x 1011 with particle sizes ranging from 30-240 nm. Kit 2 yielded a value of 2.9 x 1010 with particle sizes ranging from 30-150 nm, while kit 3 had no measurable value. The average concentration of RNA extracted using the RNA Basic kit from HansaBioMed was 42.53 ng/μL, while the exoRNeasy Midi kit from Qiagen yielded 45.28 ng/μL. Template RNA of 100 ng was converted into cDNA, while the cDNA was diluted at a ratio of 1:20 using RNase free water during the quantification process of miRNA exosome expression with the qRT-PCR method. Based on the results, exosome miRNA quantification process requires the use of the correct method at each stage to obtain the right expression for clinical studies.
Co-Authors . Suharno Adeodatus Yuda Handaya Aditya Rifqi Fauzi Agus Supartoto Ahmad Hamim Sadewa Ahmad Zulfan Ahmad Zulfan Ajeng Viska Icanervilia Alberta Vania Handoko Alfasunu, Serafim Alharsya Franklyn Ruckle Alva Sinung Anindita Andreas Pramudito Andrew Nobiantoro Gunawan Anwar, Sumadi Lukman Aprilia Indra Kartika Aria Danurdoro Auliya Suluk Brilliant Sumpono Azriel Farrel Kresna Aditya Bening Rahimi Titisari Brigitta Natasya Halim Bustanul Ardianto Camelia Herdini Danarto Danarto Danarto Danarto Danarto Danarto Datu Respatika Dhimas Hari Sakti Diah Rumekti Hadiati Dibyo Pramono Endang Soetristi Ery Kus Dwianingsih Ferronika, Paranita Frannata, Lucky Hanafi, Arif Riswahyudi Hendra Susanto Ida Ayu Setyawati Sri Krisna Dewi idha safitri Imelda, Priscillia Indra Tri Mahayana Indrawarman Soeroharjo Indrawati Indwiani Astuti Indwiani Astuti Indwiani Astuti Irianiwati Widodo Isadora Gracia, Isadora Jayusman, Achmad Mulawarman Joshua Andrew Junaedy Yunus Juwita Raditya Ningsih Kamal Agung Yudayana Kartika Widayati Taroeno-Hariadi Kevin Radinal Lina Choridah Mauny, Muhammad Puteh Melita Suwan Djaja Muchamad Dafip Muhammad Bayu Sasongko Muhammad Javedh Iqbal Muhammad Puteh Mauny Muhammad Puteh Mauny Mustofa Mustofa Nanda Qoriansas Naomi Yoshuantari Nastiti Wijayanti Neneng Ratnasari Novan Adi Setyawan Nur Arfian Nur Arfian Nur Signa Gumilas Nurlaila, Prima Sugesty Pawiroranu, Suhardjo Puji Lestari Putri, Rachma Greta Perdana Rachma Greta Putri Raden Danarto Rengganis, Anggraeni Ayu Renovaldi, Dede Rita Cempaka Ryan Feraldy Haroen Sabirin, Rahmaningsih Mara Sagung Rai Indrasari Sakti Ronggowardhana Brodjonegoro Sangundo, Muhammad Faham Sari Eka Pratiwi Sasongko, Muhammad Bayu Setyo Purwono Siti Boedina Kresno Sofia Mubarika Haryana Sofia Mubarika Haryana Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Sumoro, William Supartoto, Agus Supriatno Totok Utoro Vincent Laiman Vincent Lau Wahyu Tri Widayati Widhasari, Idhayu Anggit Yudi Susanto Yuliani, Fara Silvia