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All Journal Jurnal Ilmu Dasar Jurnal Lahan Suboptimal Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Jurnal Penelitian Pertanian Terapan Panrita Abdi - Jurnal Pengabdian pada Masyarakat AGRISAINTIFIKA Jurnal Ilmu-ilmu Pertanian Agrin : Jurnal Penelitian Pertanian SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Journal of Tropical Crop Science Jurnal Pengabdian Magister Pendidikan IPA Jurnal Agroteknologi dan Pertanian (JURAGAN) BIOVALENTIA: Biological Research Journal PAPUMA: Journal of Comunnity Services Jurnal Agroteknologi dan Pertanian (JURAGAN)
Laily Ilman Widuri, Laily Ilman
Program Studi Agronomi, Fakultas Pertanian, Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : BIOVALENTIA: Biological Research Journal

 1 
A SIMPLE PROTOCOL FOR SOMATIC EMBRYOGENESIS INDUCTION OF IN VITRO SUGARCANE ( Saccharum officinarum. L) BY 2,4-D AND BAP Widuri, Laily Ilman; Dewanti, Parawita; Sugiharto, Bambang
BIOVALENTIA: Biological Research Journal Vol. 2 No. 1 (2016)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.079 KB) | DOI: 10.24233/BIOV.2.1.2016.32

Abstract

Induction of in vitro sugarcane through somatic embryogenesis technique influenced by addition of plant growth regulator. The objective of this research was to determine appropriate formulation medium for indirect somatic embryogenesis induction on two potential sugarcane SUT Event 02 and PS 881. This research carried out in three steps, callus induction, callus proliferation, and shoot regeneration. Explants taken from basal of in vitro plantlet one month SUT Event 02 and PS 881 resulted from shoot regeneration previously. Five different medium formulas applied for callus induction and one formula for proliferation and shoot regeneration. Research using completely randomized design (CRD) factorial with five different formulation induction mediums. The result showed that the best respond of indirect somatic embryogenesis on SUT Event 02 and PS 881 was medium containing  3 mgL-1of  2,4-D.
PERFORMANCE OF SOMATIC EMBRYOGENESIS DEVELOPMENT UNDER DIFFERENT 2,4-D AND COCONUT WATER CONCENTRATION IN SUGARCANE VAR. BULULAWANG Dewanti, Parawita; Maryam, Safira Arikha; Widuri, Laily Ilman; Okviandari, Purnama
BIOVALENTIA: Biological Research Journal Vol. 6 No. 1 (2020)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (603.025 KB) | DOI: 10.24233/BIOV.6.1.2020.155

Abstract

Mass propagation technology through somatic embryogenesis has become an alternative for producing sugarcane seedlings rapidly.Application of proper plant growth regulator and concentration contribute to support somatic embryogenesis development. This study applied the combination of liquid and solid culture during proliferation stage to promote cell dispersion of embryogenic callus, rapid  embryo somatic production, and improve regeneration potency of somatic embryo. Application of 2,4-D and coconut water during proliferation may expected as proper combination for accelerating somatic embryo development and regeneration.Development of somatic embryogenesis in sugarcane var. Bululawang during proliferation were described in this study. Embryogenic callusfrom induction media were transferred to proliferation media containing MS Basal + vitamin supplemented with sucrose different level of 2,4-D (1 mgl-1, 2 mgl-1, 3 mgl-1, 4 mgl-1 ) and coconut water (0% and 5%).Result showed that low concentration of 2,4-D induced optimum somatic embryogenesis development in proliferation and regeneration. Concentration of single 2,4-D 1 mgl-1 without coconut water  induced rapid development of scutelar and coleoptilarduring proliferation and resulted in better shoot regeneration. In other way, 4 mgl-1 of 2,4-D concentration  affected to inhibit scutelar and coloeptilar formed as the result of failure callus differentiation.
DIFFERENT NITRATE AND AMMONIUM LEVELS MEDIA ON CHANGES OF NITROGEN ASSIMILATION ENZYMES IN RICE Handoyo, Tri; Ilman Widuri, Laily; Pudji Restanto, Didik
BIOVALENTIA: Biological Research Journal Vol. 7 No. 1 (2021)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.901 KB) | DOI: 10.24233/biov.7.1.2021.204

Abstract

Nitrogen (N) is an important nutrient for the growth and development of rice plants, required in large quantity and often limiting factor of rice yields. The research was to understand the different sources and levels of nitrogen in rice plant on the activity of N assimilation enzymes, including nitrate reductase (NR), glutamine synthase (GS) content, glutamate synthase (Gogat) content, content, ammonium (NH4+) and nitrate (NO3-) content on the leaves. Paddy (Ciherang variety) was grown in sand media containing Hoagland solution with different sources (ammonium and nitrate) and levels (0.4, 0.8, 1.6, 3.2, 6.4, and 12.8 mM) of nitrogen. Nitrogen assimilation was observed from leaves at one month of age. The NR activity increased on both Nitrogen sources, it was a higher activity in media contained nitrate. Also, the activity of GS showed higher in media contains nitrate, but its activity was decreased after application 1.6 mM of nitrate and 3.2 mM of ammonium. Western blot analysis of GS1 and GS2 showed that the band pattern of protein was similar to these enzyme activities. Nitrate content in leaves gradually increased in both sources of nitrogen and higher than 3.2 mM ammonium application caused an increase in ammonium content in leaves, but the nitrate content decreased. This research resulted that the available source of N for rice was in nitrate form, easily by the rice plants during the growth stage.
Co-Authors . Usmadi Agustin, Vira Audita Alfarisy, Fariz Kustiawan Amalia, Dita ANDI WIJAYA Astuti Kurnianingsih Bambang Kuswandi Bambang Sugiharto Benyamin Lakitan Erizal Sodikin Erna Siaga, Erna Evita Soliha Hani Fifteen Aprila Fajrin Firdauzi, Sandy Al Firdha Narulita Alfian Hadi Paramu Harsanti, Restiani Sih Ibanah, Indah Indri Fariroh Julian Adam Ridjal Kadir, Sabaruddin Kartika Kartika Kartika Kartika Kartika Kartika Kartika Kartika Khozin, Mohammad Nur Lindiana Lindiana M. Umar Harun Magfiroh, Illia Seldon Maryam, Safira Arikha Mei Meihana Mei Meihana Meihana Meihana Meihana, Mei Mery Hasmeda Nurhasna, Rifqi Parawita Dewanti Pudji Restanto, Didik Purnama Okviandari, Purnama Raisah Bani Ratih Apri Utami Restanto, Didik Pudji Rofiqi, Ahmad Rusdiana, Riza Yuli Sanjaya, Nanda Sigit Soeparjono SITI HERLINDA Slameto Slameto Soliha Hani, Evita Susilawati, Susilawati Tri Agus Siswoyo TRI HANDOYO Tri Saputra, Tri Tri Wahyu Saputra Wildan Muhlison, Wildan Yuli Witono Yulianto, Roni