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St. Maryam, St.
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ANTIOXIDANT ACTIVITY TEST OF THE ETHANOL EXTRACT OF AVOCADO SEED (Persea americana Mill.) USING THE FRAP METHOD Ohorela, Indah Kartika; Maryam, St.; Suhaenah, Asriani
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.221

Abstract

Avocado seeds (Persea americana Mill.) are a plant that is useful as a traditional medicine. The ethanol extract of avocado seeds contains several secondary metabolite compounds, namely alkaloids, triterpenoids, tannins, flavonoids and saponins. The aim of this research was to determine the antioxidant activity contained in avocado seed extract (Persea americana Mill.). using the FRAP method which was measured with a UV-Vis spectrophotometer at a wavelength of 794 nm. Extraction of avocado seeds (Persea americana Mill.) using the maceration method using 96% ethanol solvent. The results show that the antioxidant activity of the ethanol extract of Avocado Seeds is 42.448 mgQE/g of extract, which means that every 1 gram of ethanol extract of avocado seeds (Persea americana Mill.) is equivalent to 42,488 mg of quercetin.
Analisis Kadar Senyawa Polifenol Dari Teh Daun Tanaman Cacao (Theobroma cacao L.) Secara Spektrofotometri UV-Vis Desi, Nilma Sri; Maryam, St.; Tahir, Masdiana
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.242

Abstract

. Cocoa leaves (Theobroma cacao L.) contain secondary metabolites such as polyphenol compounds, flavanoids, saponins and tannins which are the largest group of phenolic compounds that act as antioxidant compounds. This study aims to determine the levels of polyphenolic compounds produced from cocoa leaf tea preparations (Theobroma cacao L.) based on the comparison of growing places, namely the Wajo, Jeneponto and Malino regions. Quantitative analysis using UV-Vis spectrophotometric method. based on qualitative analysis by Thin Layer Chromatography method using mobile phase n- hexanes: ethyl acetate: methanol with a ratio (2: 7: 2). Based on the results showed that the preparation of cocoa leaf tea (Theobroma cacao L.). As well as quantitative analysis measured at a maximum wavelength of 768 nm with gallic acid comparator standard. Tea samples contain polyphenolic compounds based on the Rf value equivalent to gallic acid comparator of 0.6. based on the analysis of levels from the Wajo area of 16.238 mgGAE/g, Jeneponto area 18.102 mg/GAE/g, and Malino area 6, 752 mgGAE/g and rf value of gallic acid comparator 0.630; rf value of samples from Jeneponto area 0.6; Wajo 0.6 and Malino 0.615. From the above results, it can be concluded that there are polyphenol compounds in tea samples from each region and the largest polyphenol content is found in the Jeneponto area.
Analisis aktivitas perlindungan sinar UV ekstrak etanol daun kakao (Theobroma cacao L.) berdasarkan nilai Sun Protection Factor. Rustam, Julis Tina; Maryam, St.; Suhaenah, Asriani
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.243

Abstract

UV rays have a bad impact on the skin, but they can be minimized by using ingredients that are UV protective. Sunscreen can be obtained from natural ingredients, one of which is Cocoa Leaves (Theobroma cacao L.) which contains flavonoid and phenolic compounds. The aim of this research was to determine the UV protection activity of ethanol extract of cocoa leaves (Theobroma cacao L.) originating from the Malino, Jeneponto and Wajo areas based on the Sun Protection Factor value. Measurement of the Sun Protection Factor (SPF) value using the UV-Vis Spectrometry method was measured at wavelengths of 290, 295, 300, 305, 310, 315 and 320 nm. The results showed that the ethanol extract of cocoa leaves (Theobroma cacao L.) had UV protection activity for the Malino area at a concentration of 0.01% of 3.25 (minimum protection), a concentration of 0.03% of 10.34 (maximum protection). , 0.05% concentration of 17.10 (ultra protection). For the Jeneponto area, the 0.01% concentration is 3.88 (minimum protection), the 0.03% concentration is 12.3 (maximum protection), the 0.05% concentration is 20.18 (ultra protection) and the Wajo area has a value at a concentration of 0.01% it is 3.38 (minimum protection), a concentration of 0.03% is 10.77 (maximum protection), a concentration of 0.05% is 17.05 (ultra protection). Ethanol extract of cocoa leaves (Theobroma cacao L.) originating from Jeneponto has the greatest UV protection activity with a value of 20.18 with ultra protection ability.
AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL RIMPANG KUNYIT (Curcuma domestica Val.) SECARA IN VITRO RAHMAN, ANDI AMALIA RAMADHANI; Maryam, St.; Razak, Rais
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.247

Abstract

Kunyit merupakan salah satu tanaman rempah-rempah yang memiliki berbagai aktivitas farmakologis. Rimpang kunyit (Curcuma domestica Val.) mengandung kurkumin dan minyak atsiri yang bermanfaat sebagai antioksidan, antiinflamasi, dan antibakteri. Tujuan penelitian ini yaitu untuk mengetahui potensi rimpang kunyit sebagai antiinflamasi secara in vitro menggunakan metode penghambatan denaturasi protein. Penggunaan metode ini karena salah satu penyebab inflamasi adalah terjadinya denaturasi protein pada jaringan sel tubuh. Hasil uji berupa persentase inhibisi dengan nilai 38,38% pada konsentrasi 10 ppm hingga 46,51% pada konsentrasi 50 ppm. Persentase inhibisi >20% menunjukkan bahwa ekstrak dapat menghambat denaturasi protein. Perhitungan IC50 menunjukkan nilai sebesar 46,87 μg/mL. Sehingga dapat disimpulkan bahwa ekstrak etanol 96% rimpang kunyit memiliki potensi sebagai antiinflamasi.
UJI AKTIVITAS ANTIOKSIDAN DARI TEH DAUN TANAMAN KAKAO (Theobroma cacao L.) BERDASARKAN TEMPAT TUMBUH DENGAN METODE PEREDAMAN RADIKAL BEBAS DPPH Syam, Nurizzah Aulia; Maryam, St.; Baits, Muzakkir
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.267

Abstract

Tanaman kakao merupakan salah satu tanaman yang digunakan oleh masyarakat sebagai obat tradisional. Daun tanaman kakao mengandung metabolit sekunder yaitu flavonoid,saponin dan tanin serta mengandung senyawa fenolat, yang juga memiliki peran sebagai antioksidan. Tujuan dari penelitian ini untuk mengetahui aktivitas antioksidan dan menganalisa IC50 dari teh daun tanaman kakao (Theobroma cacao L.) dengan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil). Sampel yang digunakan berasal dari tiga daerah yaitu Jeneponto, Wajo, dan Malino. Berdasarkan hasil penelitian, menunjukkan bahwa sediaan teh daun kakao (Theobroma cacao L.) memiliki aktivitas sebagai antioksidan. Nilai IC50 sediaan teh daun kakao (Theobroma cacao L.) Jeneponto sebesar 187,946 µg/mL, Wajo 309,629 µg/mL, dan Malino sebesar 314,055 µg/mL dengan kategori klasifikasi sangat lemah. Sediaan teh daerah Jeneponto memiliki aktivitas antioksidan yang lebih baik dari dua daerah lainnya. Kata kunci: Antioksidan, DPPH (2,2-difenil-1-pikrilhidrazil), Theobroma cacao L., Kuarsetin.
UJI AKTIVITAS ANTIOKSIDAN PADA EKSTRAK ETANOL DAUN KAKAO (Theobroma cacao L.) DENGAN PERBANDINGAN BERDASARKAN TEMPAT TUMBUH DENGAN METODE PEREDAMAN RADIKAL BEBAS DPPH Salahuddin, Muthia Amaliah; Maryam, St.; Tahir, Masdiana
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 2 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i2.268

Abstract

Kakao (Theobroma cacao L.) merupakan salah satu tanaman yang mengandung senyawa metabolit sekunder yaitu flavonoid, saponin dan tanin. Secara empiris daun kakao digunakan sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan ekstrak etanol daun kakao (Theobroma cacao L.) dengan perbandingan berdasarkan tempat tumbuh menggunakan metode peredaman radikal bebas DPPH (2,2-Difenil-1-Pikrilhidrazil) dengan parameter nilai IC50. Berdasarkan hasil yang diperoleh, hasil uji kualitatif menunjukkan adanya metabolit sekunder yang terkandung dalam esktrak etanol daun kakao seperti alkaloid, fenolik, flavonoid, saponin, dan tanin serta diperoleh nilai IC50 ekstrak etanol daun kakao daerah Jeneponto sebesar 24,658 µg/mL kategori sangat kuat, daerah Malino 59,742 µg/mL kategori kuat, serta Wajo 56,898 µg /mL kategori kuat dengan perbandingan baku pembanding yaitu kuersetin dengan nilai IC50 sebesar 1,882 µg/mL kategori sangat kuat. keyword: Theobroma cacao L., antioksidan, IC50;
Antioxidant Activity Aktivitas Antioksidan Ekstrak Etanol Daun Lerak (Sapindus rarak DC) Dengan Metode Peredaman Radikal Bebas DPPH Asri, Fitriani; Suhaenah, Asriani; Maryam, St.
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 3 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i3.289

Abstract

FITRIANI ASRI. Antioxidant Activity Of Ethanol Extract Of Lerak eaves (Sapindus rarak) Using The DPPH Free Radical Reduction Method (Supervised by Asriani Suhaenah dan St. Maryam) Lerak leaves (Sapindus rarak) are a traditional plant used by the community to treat various diseases. The secondary metabolic compounds contained in the lerak plant (Sapindus rarak) which have potential as antioxidants are flavonoids, alkaloids, saponins, tannins, quinones, steroids and triterpenoids which are obtained through extraction using ethanol solvent. This research aims to determine the antioxidant activity of the ethanol extract of lerak leaves (Sapindus rarak) using the DPPH free radical reduction method (1,1-diphenyl-2-picrihydrazyl). The antioxidant value was measured using UV-Vis spectrophotometry at a wavelength of 516 nm. The calculation results show an IC50 value of 876,592 μg/mL (very weak category) while quercetin as a comparison has very strong antioxidant activity with an IC50 value of 1,882 μg/mL.
Analisis Kandungan Kimia Dan Penetapan Kadar Polifenol Daun Lerak (Sapindus rarak DC) Dengan Metode Spektrofotometri UV-Vis Salim, Medina; Maryam, St.; Muflihunna, A.
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 3 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i3.290

Abstract

MEDINA SALIM. Chemical Content Analysis and Determination of Lerak Leaf Polyphenol Content (Sapindus rarak DC) by UV-Vis Spectrophotometry Method (Guided by St. Maryam and A. Muflihunna). Lerak contains polyphenolic compounds that reduce blood sugar levels. This study aims to determine the chemical content contained in lerak leaf extract (Sapindus rarak DC) and find out how much polyphenol compounds in the sample. The samples were macerated for 3 days using 96% ethanol solvent, the extraction process was carried out until obtaining an extract yield of 9.323 grams, then two tests were carried out, namely qualitative and quantitative, The qualitative test uses a wet reagent while the quantitative test uses the UV-Vis spectrophotometry method. The maximum wavelength used was 768 nm with gallic acid as a comparison. Based on the study, the results of ethanol extract of lerak leaves (Sapindus rarak DC) contain secondary metabolites, namely saponins, steroids, polyphenols and tannins and the result of polyphenol content is 21,168 mgGAE/g.
analisis kadar analisis kadar senyawa tanin pada teh daun tanaman kakao (theobroma cacao L.) secara spektrofotometri UV-Vis arifin, nurrizky afiah; Suhaenah, Asriani; Maryam, St.
Makassar Pharmaceutical Science Journal (MPSJ) Vol 2 No 3 (2024)
Publisher : PHARMACY UMI JOURNAL SYSTEM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/mpsj.v2i3.291

Abstract

Cocoa leaves (Theobroma cacao L.) contain secondary metabolite compounds, namely flavonoids, saponins, tannins, theobromine, caffeine, anthocyanins, leucoanthocyanins and catechol. This research aims to determine the levels of tannin compounds contained in tea preparations from the leaves of the cocoa plant (Theobroma cacao L.) based on a comparison of growing places, namely Wajo, Jeneponto and Malino. The research was carried out qualitatively using wet reagents and quantitatively using the UV-Vis spectrophotometric method. Based on the qualitative test results, FeCl3 1% reagent produces a green solution blackish, the gelatin test produces precipitation, while the potassium ferricyanide and ammonia reagent produces a dark brown color, these results indicate that cocoa leaves contain tannin compounds, for the quantitative test it is measured using UVVis spectrophotometry at a maximum wavelength of 772 nm with a reference, namely gallic acid. . The results obtained for the Wajo area were 1,618mgGAE/g, Jeneponto 2,334mgGAE/g, and Malino 1,030mgGAE/g, based on these data it can be concluded that cocoa leaves contain tannin compounds and the highest levels are in the Jeneponto area.
Co-Authors A. Muflihunna, A. Agustiani, Eka Agustin, Verren Regina Akung Daeng Alodya, Baiq Putri amelia yolanda, amelia Aminah Aminah Annisa, Nurfiqah arifin, nurrizky afiah Arini, Gusti Ayu Asri, Fitriani Asriani Suhaenah Azzahra, Resqinah B, Moh Baktiar Desi, Nilma Sri EMI SALMAH Fajar, Suwandy Fitriana Fitriana Fuady, Helmi Hidayanti, Nurul Hidayat, M. Ali Akbar Indriyani , Maulida Irwan Irwan Irwan, M Irwan Iwan Harsono Khoirul Umam Lambang Basri Said M. Firmansyah M. Irwan M. Irwan, M. Irwan Masdiana Tahir Mayanah, Dewi Muhammad Awaludin Muhammad Firmansyah, Muhammad Mustika, Nurfadila Muzakkir Baits, Muzakkir Nur Awwalunnisa, Nur Ohorela, Indah Kartika p, irmawan jamal Parobi, Rizki Prima Astuti Handayani Putra, Iwan RAHMAN, ANDI AMALIA RAMADHANI Rahmawati, Rahmawati Rais Razak, Rais Ramdani, Ridho Rekhan. T, Muh Rafli Rusniyanti, Rusniyanti Rustam, Julis Tina Sahri Sahri Salahuddin, Muthia Amaliah Salim, Medina Sasliani, Sasliani Satarudin Siti Helmyati Syam, Nurizzah Aulia Sya’baniyah Herman, Nur Fajriah Wahidin Wahidin Wahyudi, M. Ilham Wiwik Handayani