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Effects of Lycopene Treatments on Development, Hatchability, and Heart Rate of Zebrafish Embryos under Heat Stress Exposure Tuska, Habib Syaiful Arif; Salsabila, Odifiannisa Ayu; Lonameo, Bonick Kartini; Hardian, Andreas Bandang; Lesmana, M. Arfan; Yesica, Reza
Veterinary Biomedical and Clinical Journal Vol. 6 No. 1 (2024)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2024.006.01.1

Abstract

Lycopene, a potent antioxidant predominantly found in red-hued fruits and vegetables such as tomatoes, has been incorporated into embryonic maturation media in vitro, demonstrating enhancements in embryo quality. Despite these advancements, the specific effects of lycopene on Zebrafish embryo quality remain unexplored. This study aims to investigate the impact of lycopene (0.65 ppm) on the development of Zebrafish embryos, focusing on hatchability (HA), and heart rate (HR) under conditions of heat stress (HS). Fertile Zebrafish embryos at the gastrula stage were induced HS (30oC; 1 hour). The embryos will be segregated into two groups: those exposed to HS and nonHS (28oC). Each group will receive one of three treatments (four replicates): control / without lycopene (P1), 2μL lycopene (P2), and 4μL lycopene (P3). Observations using an inverted microscope was done every three up to 48 hours post-fertilization (hpf), and subsequently every five hours up to 96 hpf. Quantitative data obtained for HA and HR were analyzed with SPSS, One-Way ANNOVA (p<0.05). The antioxidants contained in lycopene can influence the speed and success of Zebrafish embryo development and HA exposed to HS. In addition, the antioxidant in lycopene can also suppress the HR frequency of Zebrafish embryos exposed to HS. However, more studies about the optimum dose, purity, and stability of the lycopene extract should be done to make a definitive conclusion.
Lipoma Removal Surgery in White-Rumped Shama (Kittacincla malabarica macraoura) Rickyawan, Nofan; Hardian, Andreas Bandang; Cadiwirya, Pradhana Kurniawan
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.275-280

Abstract

Lipoma in birds is a benign tumor of adipose cells that often occurs in the subcutaneous area. White-rumped shama, more than three years old, was rushed to the puskesmas with symptoms of decreased activity and singing ability. The results of the examination were two round, multi-focal masses, firmly demarcated, yellowish in color with a soft, chewy consistency measuring ± 4 x 2 x 1 cm on the chest and ± 1 x 1 x 1 cm. in the abdominal area, firmly attached to the subcutaneous layer since six months ago, and getting bigger. The feathers around the mass are shed and vascularized on the surface of the mass. Surgery was performed because the mass suspected of being a tumor growing rapidly and causing pain. It was fasted two hours before surgery and the anesthetic used was a combination of ketamine HCl and xylazine. Postoperative therapy using the antibiotic enrofloxacin, ketoprofen analgesics, 0.9% NaCl fluid therapy and topical antibiotic gentamicin ointment. The tumor that has been successfully removed is yellowish in color with several areas of hyperemia and an oily surface. The results of histopathological examination characterized by the presence of adipose cells that appeared to proliferate well confirmed that the tumor was a lipoma.
The Association of Age and Number of Parturition with Mammary Gland Tumor Case Rate in Mice in Malang Raya Wukirani, Maulidi Robingi Mardiyani; Adha, Essly Hervianingsih; Dewi, Sang Ayu Putri Aristya; Setianingrum, Ani; Pratama, Dyah Ayu Oktavianie Ardhiana; Hardian, Andreas Bandang
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.9-15

Abstract

Mammary gland tumours are frequently reported in adult female mice. It broadly affects the productivity and breeding performance of mice in breeding centres. The tumours might occur spontaneously due to internal factors such as genetic mutation and hormonal disturbance, however, external causes such as Mouse Mammary Tumour Virus (MMTV) infection was also reported. This study aimed to prove that age and number of parturition associate with mammary gland tumour case rate in female mice in Malang Raya. Chi-square tests were employed to analyse the association between risk factors and mammary gland tumour case rate in this study. Histopathologic analysis described three subtypes of mammary gland tumours in mice which were adenoma, adenocarcinoma type A and adenocarcinoma type B. Chi-square tests results of tumour case rate toward age and number of parturitions were consecutively 0,004 and 0,0025 which indicated that age and number of parturitions were associated with mammary gland tumours case rate in mice in Malang Raya. The mapping of tumour case distribution visually showed a trend that higher disease proportions were spotted in higher altitude of sampling location. This might relate to the geographical breeding characteristics or temperature which needs further study to explore the association of spatial factors with the mammary tumour incidence in mice.
Efficacy of Oregano Extract Ointment on Fibroblast Cells and Epidermis in Albino Rats with Excisional Wound Model Lesmana, Muhammad Arfan; Amri, Indah Amalia; Cahyanissa, Ardilla Rachma; Geoputri, Kartika Myrnandari; Anisa, Agri Kaltaria; Hardian, Andreas Bandang
Jurnal Medik Veteriner Vol. 6 No. 2 (2023): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss2.2023.262-270

Abstract

This study aimed to determine the effect of oregano extract ointment therapy on the number of fibroblast cells and the epidermal thickness in excised wounds. The experimental animals used were 20 male Wistar strain albino rats, 12 weeks old, weighing 100–150 g, divided into 5 treatment groups i.e., (C-) not excised and without ointment, (C+) excised and without ointment, and the treatment group were excised using a 5 x 5 mm biopsy punch and treated with oregano extract ointment using concentrations of (T1) 3%, (T2) 6% and (T3) 9% twice daily for 14 days in an excised wound. The variables observed in this study were the number of fibroblast cells and the epidermal thickness by the HE staining method then measured using ImageJ. Data analysis used the One-Way ANOVA test followed by the Tukey test (p < 0,05). The results showed a decrease in the number of fibroblast cells and an increase in the epidermal thickness in the 6% concentration ointment. This study concluded that the administration of oregano extract ointment with a concentration of 6% was the optimal concentration in accelerating the final proliferative phase, characterized by a decrease in fibroblast cells and an increase in epidermal thickness.
Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction Yesica, Reza; Hardian, Andreas Bandang; Oktanella, Yudit; Kusumarini, Shelly; Prasetyo, Gabriela Jeanne Giacynta
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.335-348

Abstract

Murine Trypanosomiasis is a disease caused by the blood protozoan Trypanosoma lewisi in rats, with the transmission process mediated by the flea species Xenopsylla cheopis and Nosopsyllus fasciatus. Cases of trypanosomiasis have been documented due to Trypanosoma lewisi infecting rats and humans in various countries. Diagnosis of T. lewisi is typically conducted using polymerase chain reaction (PCR), which amplifies target DNA using specific primers. One such target gene for detection is the Internal Transcribed Spacer-1 (ITS1). Subsequent sequencing of PCR products enables analysis of genetic variation employing parameters such as nucleotide composition, genetic distance, and phylogenetic analysis with MEGA software. Test results based on percent identity values indicated a 98.51% homology of blood samples with the Chinese strain of T. lewisi (FJ011094.1), demonstrating genetic variation. Phylogram reconstruction revealed that samples 18, 19, and 37 of T. lewisi exhibit very close intraspecies relationships with T. lewisi from NCBI genebank with genetic distance ranging from 0.007 to 0.01. While the closest interspecies relationship was found with T. cruzi (KT305857.1) with a genetic distance of (d = 0.61).
Distinct Island Lineages of Binturong (Arctictis binturong) from Indonesia and Its Conservation Implications Hardian, Andreas Bandang; Rahmawati, Irhamna Putri; Widayanti, Rini; Aninta, Sabhrina Gita
HAYATI Journal of Biosciences Vol. 31 No. 5 (2024): September 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.5.916-928

Abstract

Binturong (Arctictis binturong) is a threatened carnivore that inhabits the forests of South and Southeast Asia. Despite its wide range, binturong is relatively scarce across its habitat distribution and is currently under the threat of poaching and illegal trade. Captive breeding has unfortunately been conducted rather haphazardly with a lack of origin record maintained, implicating potential risks to the management such as inbreeding or genetic swamping. This study thus aims to characterise the phylogenetic relationship of Indonesian binturong within the context of Southeast Asian binturong and further probe the distinctness of lineages originating from Java, Sumatra, Indonesian Borneo, and Bangka using Cytochrome B (CytB) and Cytochrome C Oxidase Subunit 1 (CO1). Genetic distance, phylogram topology, and haplotype analysis of both encoding genes further corroborate the distinctness of Java, Borneo, and Bangka binturong from other binturong from Indochinese regions such as India, Laos, and Myanmar. Search for prospective single nucleotide polymorphism markers to discriminate island lineages consistently found that each Java, Bangka, and Bornean binturong be distinct from each other and other lineages, especially when assessed using haplotype-based clustering. Assigning binturong originated from Sumatra is nonetheless more complicated, suggesting the possibility. Our findings substantiated the much-needed systematic research of Southeast Asian binturong as ex-situ insurance population management grows in Indonesia and other parts of the world to protect the diversity of binturong lineages and their corresponding unique evolutionary history.
Penanganan Kasus Cystitis Hemoragik pada Kucing Mix Persian di Klinik Hewan Awal Care Ramadani, Amanda Haristia Ramadani; Mirjawal; Tiara Widyaputri; Mira Fatmawati; Andreas Bandang Hardian
Jurnal Veteriner dan Biomedis Vol. 3 No. 1 (2025): Maret
Publisher : Sekolah Kedokteran Hewan dan Biomedis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jvetbiomed.3.1.30-35.

Abstract

Hemorrhagic Cystitis is an inflammation accompanied by bleeding that occurs in the urinary bladder characterized by thickening of the urinary bladder wall and has clinical symptoms of hematuria or urine with blood, dysuria, abdominal distension and pain when palpated in the abdominal area. Patients with symptoms of weakness, loss of appetite and vomiting. An examination was carried out by a doctor and a pain response was found in the abdomen, after further observation by palpation, an enlarged VU was felt and hematuria was present. The results of the Ultrasonography examination found a thickening of the urinary bladder wall, then a microscopic examination of native urine found red blood cells. Hematology results showed an increase in parameters in leukocytes, and granulocytes and a decrease in platelets indicating acute infection and also bleeding or acute inflammation. A blood chemistry examination showed an increase in BUN and Creatinine levels caused by post-renal Azotemia. The results of the signaling and anamnesis were associated with the results of the supporting examination of the chika cat diagnosed with Hemorrhagic Cystitis and cystotomy treatment was performed. Treatment and handling are done after cystotomy surgery with treatment in the form of antibiotics as causative therapy, musculorelaxants and anti-fibrinolytics as symptomatic therapy, and multivitamins as supportive therapy. Handling is done by giving special diet feed, catheterization, and laser therapy on post-operative wounds.
Literature Study : Histopathology Analysis of Rabies Disease (2014 - 2022) Andadari, Afifah Yudining Dwi; Pratama, Dyah Ayu Oktavianie A.; Hardian, Andreas Bandang; Untari, Handayu
Journal of Applied Veterinary Science And Technology Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V5.I1.2024.93-98

Abstract

Background: Rabies is a contagious and zoonotic disease caused by Lyssavirus, a neurotropic, negative-sense, single-stranded RNA virus. Rabies infects most mammals through the transmission of saliva from a rabid animal that enters the body of a healthy animal through an open wound, bite, scratch, or direct contact. A post-mortem diagnosis and evaluation are crucial for establishing regional statistical data in order to design a robust strategy for combating rabies. Histopathology has long been known to be an invaluable source of information regarding the destructive impact of rabies in nervous tissue. In addition to other ante-mortem evaluations, a correlation between clinical signs, gross pathological findings, and histopathological findings must be established to achieve the maximum target of combating rabies. Purpose: This study aims to analyze and compare gross pathological findings and histopathological findings in the literature published between 2014 and 2022. Methods: A comparison between gross pathological findings and histopathological findings was conducted on case studies from 2014 to 2022. Anamnesis and clinical signs were obtained as supporting data. The findings were presented as percentage to identify the predominant pathological signs. Results: A review of four case studies revealed that paralysis, hyperesthesia, gait abnormality, and muscle tremor were the most commonly observed signs (50%). Only two case studies revealed gross pathological findings, including meningeal congestion and cerebellar hemorrhage. Eight studies identified the intracytoplasmic negri body in Purkinje cells as the most commonly observed histopathological finding (100%). Conclusion: The primary clinical signs and gross pathological findings associated with rabies are typically regarded as non-specific. Histopathology revealed the intracytoplasmic Negri body in Purkinje cells as the most commonly observed finding (100%).