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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology AGRIVITA, Journal of Agricultural Science Jurnal Pengabdian kepada Masyarakat Nusantara Sewagati Journal of Bio-molecule Research And Engineering
Almando Geraldi
Laboratory Of Microbiology, Department Of Biology, Faculty Of Science And Technology, Universitas Airlangga, Surabaya, Indonesia 60115 2. Research Group For Applied Microbiology And Bioresource Technology, Universitas Airlangga, Surabaya, Indonesia 6
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Immunology & microbiology Languange, Linguistic, Communication & Media Public Health Social Sciences Veterinary Other

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Journal : Journal of Bio-molecule Research And Engineering

 1 
Isolation and Characterization of Thermophilic Bacillus subtilis subsp. inaquosorum CGR-1 from Cangar Hot Springs Geraldi, Almando; Aulia Azzahra; Dimas Aryq Ijlal Wafi; Febriani Sukma Maghfirotul Chasanah; Lillah Asritafriha; Rizki Amaliah Zain; Us Watun Nurul Khasanah
Journal of Bio-Molecule Research and Engineering Vol 1 No 1 (2022)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v1i1.35860

Abstract

Bioindustries often involve biochemical processes that occur at higher temperatures. However, most proteins, including enzymes, lose their structural integrity and functionality at higher temperatures. Thus, thermostable enzymes from thermophilic microorganisms are best suited candidates for successful bioprocessing under such conditions. Indonesia is one of the best study sites for performing bioprospecting of thermostable enzyme-producing thermophilic microorganisms due to the numerous hot springs. To explore the biodiversity of thermophilic microorganisms with potential industrial applications, we isolated and characterized thermophilic bacteria from the Cangar hot spring, Batu, East Java, Indonesia. One isolate (CGR-1) showed growth at 60°C and was identified as Bacillus subtilis subsp. inaquosorum based on 16s rRNA gene sequencing followed by bioinformatic analysis. This is the first report on the isolation of Bacillus subtilis subsp. inaquosorum CGR-1 from Indonesia, especially from a hot spring environment. This isolate showed cellulolytic and amylolytic activity at 50°C, which would encourage further exploration on the industrial and environmental applications.
Potential of Microbial Isolates from Indonesia in Producing Antimicrobial Compounds: A Review Geraldi, Almando; Wijaya, Nabilla Hapsari; Clement, Christopher; Hajar, Versa Rachmania
Journal of Bio-Molecule Research and Engineering Vol 2 No 1 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i1.52001

Abstract

Indonesia, as a megabiodiverse country, possesses a wealth of beneficial microorganisms, including bacteria and fungi capable of producing antimicrobial compounds. Over the past decade, extensive research has been conducted in Indonesia to explore and screen the antimicrobial activities of bacteria and fungi. These microorganisms have been isolated from various ecosystems, such as terrestrial, maritime, extreme environments like hot springs and sand dunes, as well as from animals and plants hosts. Most studies have focused on the antimicrobial activity of crude metabolite extracts, which demonstrate inhibitory effects against clinically significant pathogens, including Methicillin-resistant Staphylococcus aureus, multidrug-resistant Escherichia coli, and Salmonella typhi. This review systematically synthesizes findings from peer-reviewed literature, detailing isolation strategies, antimicrobial screening techniques, and the bioactivities reported. By consolidating current knowledge, it aims to elucidate the potential of Indonesia’s microbial resources for developing novel antimicrobial agents and promoting their sustainable exploitation within the context of global health challenges.
Pycnoscelus surinamensis Endosymbiont Bacteria: Identification and its Amylase Characteristic Ni'matuzahroh; Fatimah; Sholikah; Ayu Tri Wahyuni; Tri Nurhariyati; Mochammad Affandi; Agus Supriyanto; Almando Geraldi; Tini Surtiningsih; Brigita Nur Diyan Agustiana; Dela Dwi Alawiyah; Ana Mariatul Khiftiyah; Silvia Kurnia Sari
Journal of Bio-Molecule Research and Engineering Vol 2 No 2 (2023)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbiome.v2i2.67571

Abstract

The amylase is an enzyme that widely used in industry. In this research, amylase was isolated from endosymbiont bacteria (EKA 4) of cockroach Pycnoscelus surinamensis from household garbage dump Surabaya. This research aims to identify bacteria EKA 4, produce and characterize amylase from it at various temperature, pH and effect of metal ions. Amylase screening assay was done by imoculating the isolate into medium contain 2% of starch then incubated for 24 hours. Detection of amylase was done qualitatively by measured presence of halozone and quantitatively using DNS method. 16S rRNA analysis found that bacteria EKA 4 has similarity to Bacillus subtilis with 100% of percent identity. Result showed that optimum activity of amylase from B. subtilis were at 40°C, pH 6 and could use ion CaCl2 as a cofactor in a reaction. Thus, utilization of amylase from endosymbiont P. surinamensis could be as an alternative source in big industries.
Co-Authors Affandi, Moch Agus Supriyanto Alfiah Hayati Ana Mariatul Khiftiyah Anjar Tri Wibowo Arneni, Wanda Dya Aulia Azzahra Ayu Tri Wahyuni Brigita Nur Diyan Agustiana Clement, Christopher Dela Dwi Alawiyah Dimas Aryq Ijlal Wafi Dwi Kusuma Wahyuni Dwi Winarni Farah Aisyah Nafidiastri Fatimah Fatimah fatimah Fatimah Febriani Sukma Maghfirotul Chasanah Febrionny, Princessa Nandita Firli Rahmah Primula Dewi Hajar, Versa Rachmania Hakimatul Husniyah Intan Ayu Pratiwi, Intan Ayu Junairiah Junairiah Lillah Asritafriha Manikya Pramudya Mochammad Affandi Muhammad Fath Alhaqqi Sanis Salamy Muhammad Hilman Fu'adil Amin Nabilatun Nisa’ Ni'matuzahroh Nimatuzahroh, Nimatuzahroh Ni’matuzahroh Putri, Elsha Nadya Marchelia Rahayuningtyas, Nazil Dwi Rizki Amaliah Zain Rizky Danang Susetyo Salamun Salamun Salamun Salamun Salsabila, Syarifah Sholikah Silvia Kurnia Sari Soelistyo, Anastasya Nabila Putri Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Tini Surtiningsih Tri Nurhariyati Tri Nurhariyati Us Watun Nurul Khasanah Wijaya, Nabilla Hapsari