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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Cancer Chemoprevention Indonesian Journal of Biotechnology Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Indonesian Journal of Pure and Applied Chemistry Pharmacoscript Jurnal Insan Farmasi Indonesia Indonesian Journal of Biological Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian Journal of Pharmaceuticals and Natural Sciences Medical Sains : Jurnal Ilmiah Kefarmasian Indonesian Journal of Chemical Science Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)
Dhania Novitasari
Departemen Analisis Farmasi Dan Kimia Medisinal, Fakultas Farmasi, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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Ethanolic extract of sappan wood (Caesalpinia sappan L.) inhibits MCF-7 and MCF-7/HER2 mammospheres' formation: an in vitro and bioinformatic study Dhania Novitasari; Laeli Muntafiah; Nur Fitra Sari; Edy Meiyanto; Adam Hermawan
Indonesian Journal of Biotechnology Vol 26, No 3 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.63510

Abstract

One of the mechanisms of cancer cell resistance toward chemotherapy is through cancer stem cells (CSCs), which are characterized by excessive activation of regulator proteins such as human epidermal receptor 2 (HER2). Sappan wood (Caesalpinia sappan L.) contains brazilin and brazilein that exhibit cytotoxic effects on several cancer cell lines. We aimed to explore the potency of the ethanolic extract of sappan (EES) in CSCs through bioinformatic analyses and by using a three-dimensional (3D) breast cancer stem cells (BCSCs) for in vitro assay with two different models (i.e., BCSCs and HER2-BCSCs) in order to identify the potential therapeutic targets of genes (PTTGs). Bioinformatic analyses identified PTTGs, which were further analyzed by gene ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, protein-protein interaction (PPI) networks, and hub protein selection. Mammospheres were cultured under conditioned media. The cytotoxic effects of EES were then measured by direct counting and based on the mammosphere-forming potential (MFP). Bioinformatic analysis disclosed PIK3CA and TP53 as PTTGs in BCSCs and HER2-BCSCs, respectively. In addition, the KEGG pathway analyses also demonstrated that PTTGs could regulate the ERBB pathway. EES thus demonstrated cytotoxicity and inhibited the formation of mammospheres. Collectively, EES exhibited excellent potential for further development as an inhibitor of cancer stem cells in breast cancer.
SMEDDS of Citrus hystrix ethanolic extract improves cardiac and hepar histopathology profile on doxorubicin-induced rats Dhania Novitasari; Prisnu Tirtanirmala; Nindi Wulandari; Layung Sekar Sih Wikanthi; Ade Safitri; Ediati Sasmito
Indonesian Journal of Cancer Chemoprevention Vol 6, No 3 (2015)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev6iss3pp97-104

Abstract

Citrus hystrix D.C. (kaffir lime) peel contains several flavonoids including rutin, naringenin, hesperidin. C. hystrix peel ethanolic extract (ChEE) has shown its potency as cardioprotector agent in chemotherapy. However, there  are  limitations  to  the  utilization  of  ChEE due to its  poor  water  solubility  and  low  oral bioavailability.  Accordingly,  self-microemulsifying drug delivery system (SMEDDS) formulations were developed to improve the oral absorption of flavonoids. Tween 80, Corn oil, and propylene glicol (5:1:1ml) were combined to form ChEE-SMEDDS. The present study is to evaluated ChEE-SMEDDS for their physicochemical  properties and in vivo using combination with doxorubicin to see blood serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), nitrit oxide (NO) activity and also cardio-hepato-histopathology of female Sprague Dawley rats. The results showed that ChEE-SMEDDS repaired cardio-hepato-histopathology profile of doxorubicin -induced rats, but did not reduce serum activity of NO, ALT and AST. These  results indicated that ChEE-SMEDDS has potency to be developed and improved as cardio-hepato-protector agent in chemotherapy.Keywords: Citrus hystrix D.C., SMEDDS, Cardio-hepatoprotector, Histopathology, Chemoterapy
Estrogenic Activity of Ethanolic Extract of Papaya Peels (Carica Papaya L.) on Uterine Weight and Mammae Gland Proliferation on Ovariectomy Rats Dhania Novitasari; Devyanto Hadi Triutomo; Fitriana Hayyu Arifah; Anselma Ivanawati; Zahrotul Ulum; Retno Murwanti
Indonesian Journal of Cancer Chemoprevention Vol 9, No 2 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev9iss2pp86-91

Abstract

Papaya bark is one of Indonesia's natural wealth that contains flavonoid compounds such as myricetin and kaempferol that included in the phytoestrogen compounds. The aim of this study is to examine the estrogenic effects of ethanolic extract of papaya peels (EEPP) on the development of mammae gland and the increasing of uterine weight. The in vivo test was performed in ovariectomized Sprague Dawley female rats. After 30 days of treatment, animals were sacrificed to take the uterus and mammae glands. Measurement of uterine weight and mammae gland was observed by hematoxylin-eosin staining method to know the lobulus development and AgNOR staining to determine the proliferation level of mammae gland epithelial cells. The results showed that EEPP at the concentrations of 500 and 1000 mg/kgBW were able to increase uterine weight and proliferation of mammae gland. In conclusion, papaya bark has the potential as phytoestrogen compound to maintain female reproductive health and woman beauty.Keywords : Ethanolic extract of papaya peels (EEPP), phytoestrogen, ovariectomized rats, uterine weight, mammae proliferation
Evaluation of The Genotoxicity of Three Food Additives using CHO-K1 Cells under in vitro Micronucleus Flow Cytometry Assay Beni Lestari; Dhania Novitasari; Herwandhani Putri; Sari Haryanti; Ediati Sasmito; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 8, No 2 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss2pp74-80

Abstract

Exposure of genotoxic substances come from various sources such as food additives. The aim of this study is to evaluate the genotoxicity of food additives in CHO-K1 cells by micronucleus test flow cytometry. The food additives: sodium saccharine (SS), monosodium glutamate (MSG), and sodium benzoate (SB) were assessed by in vitro cytotoxicity and genotoxicity using Chinese Hamster Ovary-K1 (CHO-K1) cells. The cytotoxic effect of those compounds was evaluated by MTT Assay on CHO-K1 Cells. The genotoxic evaluation was observed by in vitro micronucleus test by flowcytometry with double staining method. The results showed that the three compounds did not perform cytotoxic effect, increased the frequency of micronucleus, and changed the cell cycle profiles. In general, these studies obtained that none of three food additives showed cytotoxic and genotoxic effect on CHO-K1 cells. Micronucleus test using flow cytometry is suitable for this purpose study.Key words : food additives, genotoxic, cytotoxic, micronucleus
Ethanolic Extract of Hedyotis corymbosa L. Inhibits Migration and MMP-9 Activity on Metastatic Breast Cancer Cells Dhania Novitasari; Sri Handayani; Riris Istighfari Jenie
Indonesian Journal of Cancer Chemoprevention Vol 9, No 1 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev9iss1pp16-22

Abstract

Many natural products have been widely explored for their pharmacological activities, including anticancer activity. Hedyotis corymbosa L. is known for its anticancer properties toward several cancer cell lines. The study aims to investigate whether ethanolic extract of Hedyotis corymbosa L. (EEH) performs anticancer properties by inhibiting migration and metastasis on breast cancer cells. Cytotoxic evaluation by using MTT assay was carried out to determine EEH effect on 4T1 breast cancer cells, meanwhile to investigate the treatment of EEH in migration and metastasis inhibitory effect, scratch wound healing assay and gelatin zymography were conducted in this study. The data showed that EEH possessed cytotoxic activity with IC50 value of 400 μg/mL.  Interestingly, migration inhibitory effect was shown up to 42 hours and the activity of MMP-9 was also decreased after the treatment with EEH. According to these findings, we suggest that Hedyotis corymbosa L. promotes another anticancer properties by inhibiting migration and metastasis towards breast cancer cells.Keywords : Hedyotis corymbosa L., cytotoxicity, migration, metastatic, 4T1 breast cancer cells
Curcumin Analogs PGV-1 and CCA-1.1 Induce Cell Cycle Arrest in Human Hepatocellular Carcinoma Cells with Overexpressed MYCN Moordiani Moordiani; Dhania Novitasari; Ratna Asmah Susidarti; Muthi' Ikawati; Jun-ya Kato; Edy Meiyanto
The Indonesian Biomedical Journal Vol 15, No 2 (2023)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v15i2.2147

Abstract

BACKGROUND: Liver cancer is the third leading mortality in cancer. Curcumin shows effective anticancer potency against various cancer including liver cancer. The synthesized curcumin analog compounds Pentagamavunone-1 (PGV-1) and Chemoprevention Curcumin Analog-1.1 (CCA-1.1) have been well studied in breast, leukemia, and colon cancer cells with better potency than curcumin itself, yet their cytotoxic activities were not known in liver cancer cells. Thus, this study was conducted to elevate the anticancer effect of these curcumin analogs against hepatocellular carcinoma (HCC) cells in vitro, specifically in MYCN-expressing cells, based on its cellular physiology.METHODS: JHH-7 cells were used as the HCC cell model with high expression of MYCN. The viability of the cells was observed using trypan blue exclusion method while cell cycle profile and intracellular reactive oxygen species (ROS) levels were quantified by means of flow cytometry. Chromosomal staining with Hoechst was applied to determine the cell cycle arrest phase, whilst X-gal staining was used to assess the cellular senescence activity.RESULTS: The result of current study presented that the growth inhibitory activity of PGV-1 as well as CCA-1.1 in JHH-7 cells was associated with the cell cycle arrest and cellular senescence. Both curcumin analogs PGV-1 and CCA-1.1 ultimately induced mitotic arrest (p<0.001) better than curcumin. Moreover, PGV-1 and CCA-1.1 similarly increased the senescent cells that partly mediated through ROS elevation. The transcription level of MYCN was not altered upon treatment with curcumin and its analogs in JHH-7 cells, suggesting that molecular mechanism of the inhibitory effect was independent from MYCN signaling.CONCLUSION: Taken together, these observations revealed that both PGV-1 and CCA-1.1 potentially serve as multi-targeted curcumin-based compounds and lead to promising anti-hepatocellular cancer agents.KEYWORDS: Curcumin analogs, hepatocellular carcinoma, mitotic arrest, MYCN
ANALYTICAL METHOD VALIDATION OF CYCLAMATE IN RED SYRUP IN NORTH BANJARMASIN USING UV-VIS SPECTROPHOTOMETER Rahmawati; Andika; Rusmina Aulia Hasanah; Dhania Novitasari
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 8 No 2 (2023)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v8i2.794

Abstract

Cyclamate is an artificial sweetener that is not allowed for public consumption, because it isspecifically permitted for diabetics and consumers with low-calorie diets. Syrup usuallyadded as a flavor enhancer (sweetener), color, and aroma. The purpose of adding artificialsweeteners to syrup is to reduce production costs because cyclamates have a highersweetness and lower price than sugar. The purpose of this study was to determine the contentand levels of cyclamate in syrup sold in various stalls and street sellers in the NorthBanjarmasin area, and to determine the data validation method with linearity, precision,accuracy, LOD, and LOQ. The results of the qualitative test showed that 4 of the 9 positivesamples contained cyclamate (samples A, C, E, and I). The results of the method validationparameter test for linearity at 20, 40, 60, 80, 100, and 120 ppm gave a value of thecorrelation coefficient (r ) of 0.9987 with an LOD value of 6.4307 ppm, LOQ of 21.4359ppm,d precision value of 0.74%, and an accuracy value of 89.5126%. The results of thequantitative tests were carried out on four samples, namely samples A, C, E, and I, withcyclamate levels of 12.9423, 31.9833, 23.4166, and 115.6469 mg/kg, respectively. Keywords: Cyclamate, Syrup, Precipitation, Validation Method, UV-Vis Spectrophotometry
ANALYTICAL METHOD VALIDATION OF CYCLAMATE IN RED SYRUP IN NORTH BANJARMASIN USING UV-VIS SPECTROPHOTOMETER Rahmawati; Andika; Rusmina Aulia Hasanah; Dhania Novitasari
Medical Sains : Jurnal Ilmiah Kefarmasian Vol 8 No 2 (2023)
Publisher : Sekolah Tinggi Farmasi Muhammadiyah Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37874/ms.v8i2.794

Abstract

Cyclamate is an artificial sweetener that is not allowed for public consumption, because it isspecifically permitted for diabetics and consumers with low-calorie diets. Syrup usuallyadded as a flavor enhancer (sweetener), color, and aroma. The purpose of adding artificialsweeteners to syrup is to reduce production costs because cyclamates have a highersweetness and lower price than sugar. The purpose of this study was to determine the contentand levels of cyclamate in syrup sold in various stalls and street sellers in the NorthBanjarmasin area, and to determine the data validation method with linearity, precision,accuracy, LOD, and LOQ. The results of the qualitative test showed that 4 of the 9 positivesamples contained cyclamate (samples A, C, E, and I). The results of the method validationparameter test for linearity at 20, 40, 60, 80, 100, and 120 ppm gave a value of thecorrelation coefficient (r ) of 0.9987 with an LOD value of 6.4307 ppm, LOQ of 21.4359ppm,d precision value of 0.74%, and an accuracy value of 89.5126%. The results of thequantitative tests were carried out on four samples, namely samples A, C, E, and I, withcyclamate levels of 12.9423, 31.9833, 23.4166, and 115.6469 mg/kg, respectively. Keywords: Cyclamate, Syrup, Precipitation, Validation Method, UV-Vis Spectrophotometry
STUDI IN SILICO METABOLIT SEKUNDER DALAM TANAMAN TAHONGAI (Kleinhovia hospita L.) SEBAGAI KANDIDAT AGEN TERAPI KARSINOMA HEPATOSELULER TERTARGET RESEPTOR c-MET (IN SILICO STUDY OF SECONDARY METABOLITES IN TAHONGAI PLANT(Kleinhovia hospita L.) AS A CANDIDATEFOR HEPATOCELLULER CARCINOMA THERAPEUTIC AGENT TARGETING c-MET RECEPTOR) Cahyaningrum, Lydia; Rubianti, Retno; Mahira, Tsania; Gabriel, Kevin; Rusdin, Agus; Novitasari, Dhania
Indonesian Journal of Pure and Applied Chemistry Vol 7, No 2 (2024)
Publisher : Tanjungpura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/indonesian.v7i2.82567

Abstract

Hepatocellular carcinoma (HCC) is the fourth leading cause of cancer-related mortality in the world, mainly caused by chronic disease or virus. Prior studies have documented that the upregulation of c-MET can trigger the cancer progression, hence c-MET has been widely explored as target therapy for HCC. Tahongai plant (Kleinhovia hospita L.) has known to possess several biological effects, including anticancer activity. However, the molecular mechanism in this plant has not been studied yet. In this study, the bioactive constituents from Tahongai were evaluated based on the physicochemical features and molecular interaction in c-MET through in silico approaches. The druglikeness of each compound was checked through SwissADME, while the pharmacokinetic profile was predicted through preADMET webtool. The pharmacophore screening and molecular docking against c-MET were assessed using LigandScout and Autodock, respectively. Out of 14 selected compounds, only one (astragalin) did not pass the Lipinski rule, and most of the compounds demonstrated good ADMET profile. Eleutherol was choosen as the hit compound based on pharmacophore studies, and stibostemin G was potential to inhibit c-MET based on similar molecular interaction compared to its native ligand through molecular docking analysis. Further confirmation is urged to prove its anticancer effect from Tahongai against HCC, particulary targeting on c-MET
In Silico Study of Active Compounds in Guava Leaves (Psidium guajava L.) toward Angiotensin Converting Enzyme (ACE) as target for hypertension Hess, Aurelina Yunita; Ramadhani, Siti Zhahira; Andhryanti, Rifa Nurfadila; Zhafirah, Noor; Muljono, Fajar Oktavian; Fardhan, Firghi Muhammad; Novitasari, Dhania
Indonesian Journal of Chemical Science Vol. 13 No. 3 (2024): Indonesian Journal of Chemical Science
Publisher : Prodi Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijcs.v13i3.8648

Abstract

Hipertensi adalah suatu kondisi medis kronis yang terjadi ketika tekanan darah melebihi batas normal sehingga dapat meningkatkan risiko komplikasi penyakit lainnya seperti penyakit jantung. Pengobatan hipertensi saat ini sebagian besar menggunakan obat golongan Angiotensin Converting Enzyme (ACE) inhibitors. Salah satu bahan alam yang memiliki potensi menurunkan tekanan darah ialah daun jambu biji. Tujuan penelitian ini adalah mengevaluasi senyawa aktif yang terdapat pada daun jambu biji terhadap interaksi secara molekuler pada protein ACE dengan pendekatan studi in silico. Metode yang digunakan dalam pengujian ini meliputi karakteristik drug likeness berdasarkan kaidah Lipinski, prediksi profil ADMET, penapisan farmakofor, dan penambatan molekul. Hasil pengujian menunjukkan senyawa asam klorogenat dan luteolin yang terkandung pada daun jambu biji memiliki interaksi baik dengan protein target ACE berdasarkan energi ikatannya. Oleh karena itu, daun biji dapat dikembangkan lebih lanjut sebagai kandidat berbasis bahan alam untuk membantu dalam terapi hipertensi.
Co-Authors Adam Hermawan Ade Safitri Aisyah Tasza Noor Alifa, Zahra Andhryanti, Rifa Nurfadila Andika Andika Anselma Ivanawati Ardhita Marshella Dhanu Ashriany, Raissa Rerey Aulia Rahman, Faaza Aulia, Martiza Azzahra, Iqlima Banun Kusumawardani Beni Lestari Cahyaningrum, Lydia CINDY CINDY Devyanto Hadi Triutomo Dwi Merry Christmarini Robin Ediati Sasmito Ediati Sasmito Edy Meiyanto Edy Meiyanto Edy Meiyanto Edy Meiyanto Fadhilah, Naya Fardhan, Firghi Muhammad Fathin Nayla Maymuna Fathin, Nayla Maymuna Fa’aizah Masayu Kyla Fitriana Hayyu Arifah Gabriel, Kevin Herwandhani Putri Hess, Aurelina Yunita Ismail, Dzava Prawinsyah Fairus Jasimah, Jasimah Jun-ya Kato Kato, Jun-Ya Kinanti, Lintang Gusti Laeli Muntafiah Lathifah, Salma Sri Layung Sekar Sih Wikanthi Lestari, Mila Ayu Maharani, Anisa Mahardika, Bintang Satrio Mahira, Tsania Masyithah Sitti Moordiani Moordiani Moulana, Mohammad Zaeni Muhammad Syahid Muljono, Fajar Oktavian Muthi Ikawati Nindi Wulandari Nunuk Purwanti Nur Fitra Sari Nurhaliza, Muthiah Oktaviana, Lina Pramudita, Fransisca Widi Prisnu Tirtanirmala Putri Salma Chiara Putri, Anindya Calista Nabila Putri, Nazwa Septiriana Putri, Viona Algesia Fiola Putri, Yolla Adellia Qurrotaayun, Ghina Alya Putri Rahmadhani Nabilah Rahmaharva, Naila Dwi Rahmawati Rahmawati Ramadhani, Siti Zhahira Ratna Asmah Susidarti Retno Ardhani Retno Murwanti Riris Istighfari Jenie Riris Istighfari Jenie Rizal Alya Izzati Zhafira Romadhona, Tarisa Nurafni Rosani, Fahrana Rubianti, Retno Rusdin, Agus Rusmina Aulia Hasanah Salsabila, Sitti Kesya Sari Fiona Puspita Sari Haryanti Shafa, Nafis Sitompul, Joy Elizabeth Nauli Sri Handayani Zahrotul Ulum Zhafirah, Noor