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All Journal Jurnal Medik Veteriner Jurnal Sylva Lestari
Pratama, Bima Putra
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Materials Science & Nanotechnology Veterinary

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Utilization of Tannins with Various Polymers for Green-Based Active Packaging: A Review Agrippina, Fidela Devina; Ismayati, Maya; Hidayati, Sri; Pratama, Bima Putra
Jurnal Sylva Lestari Vol. 12 No. 3 (2024): September
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v12i3.893

Abstract

Tannin is a major polyphenolic secondary metabolite widely distributed in the forestry sector and can be added to polymer matrices to manufacture bioactive films for food packaging. Tannins have functional properties as antioxidants, antibacterial, and anti-inflammatories. Tannins are classified into two types, namely condensed tannins and hydrolyzable tannins. Tannins have been used primarily in food, wood, leather, pharmaceutical, and other industries. In the food industry, tannins are used to develop food packaging, preservation, and the function of the food industry. Tannin molecules have the advantage of combining with polymers or polymer surfaces, for example, synthetic polymers, biopolymers, and micro- and nano-sized fibers, which will form new products whose physical and chemical properties increase in functional properties because of the presence of hydrophilic and nucleophilic groups. Combining tannins with a polymer can be done by one-step reactive extrusion, layer by layer, dynamic vulcanization, acetylation, and in situ extraction methods. This paper describes general information about tannins, followed by applications using tannins, tannin-based hybrid materials, and methods of combining tannins with a polymer for food packaging purposes. Keywords: active packaging, bioactive film, polymer, secondary metabolite, tannins
Morphometric and Molecular Identification of Eimeria Bovis and Eimeria Zuernii on Beef Cattle in Lamongan, East Java, Indonesia Kurniawan, Muhammad 'Ahdi; Suwanti, Lucia Tri; Mufasirin, Mufasirin; Suprihati, Endang; Hastutiek, Poedji; Kusnoto, Kusnoto; Ansori, Arif Nur Muhammad; Puspitasari, Yulianna; Khairullah, Aswin Rafif; Moses, Ikechukwu Benjamin; Pratama, Bima Putra; Riwu, Katty Hendriana Priscilia
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.153-166

Abstract

Lamongan Regency, located in East Java, Indonesia, is a significant center for beef cattle production. Despite its prominence, studies on the identification and differentiation of Eimeria spp. parasites in this region are notably lacking. This research aims to address this gap by evaluating the prevalence of Eimeria spp. and identifying two pathogenic species: Eimeria bovis and Eimeria zuernii. The study involved the collection of 250 fecal samples from beef cattle raised on smallholder farms across Lamongan. Sampling was conducted during the rainy season to optimize the detection of Eimeria infections. Using the sugar flotation method, Eimeria oocysts were isolated from 5–10 g of fecal matter per sample. Molecular identification employed polymerase chain reaction (PCR) assays targeting ribosomal RNA’s internal transcribed spacer 1 (ITS-1) region to detect the pathogenic species. Fecal examination using the Whitlock test revealed a prevalence of Eimeria spp. at 44.45% (111/250). PCR analysis further identified E. bovis with a 238 bp amplicon in Solokuro District and E. zuernii with a 344 bp amplicon in Tikung District, highlighting the presence of these two pathogenic species in distinct geographic areas. The findings underline the need for further research that includes expanded sampling from different regions and cattle breeds and the application of varied diagnostic methods. Such studies will contribute to a more comprehensive understanding of the genetic diversity, distribution, and epidemiology of E. bovis and E. zuernii in Indonesia, supporting improved cattle health and management practices in the region.
Detection of the iroN gene in strains of avian pathogenic Escherichia coli in ducks at the Surabaya live market Kendek, Irfan Alias; Effendi, Mustofa Helmi; Wibisono, Freshinta Jellia; Putri, Mariana Febrilianti Resilinda; Abidin, Zainul; Naseh, Naseh; Rehman, Saifur; Khairullah, Aswin Rafif; Yanestria, Sheila Marty; Pratama, Bima Putra; Kurniasih, Dea Anita Ariani; Ahmad, Riza Zainuddin; Tang, John Yew Huat; Kurniawan, Muhammad ‘Ahdi; Dameanti, Fidi Nur Aini Eka Puji
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.242-258

Abstract

Escherichia coli normally inhabits the intestinal tract of poultry as a commensal organism; however, specific strains such as avian pathogenic E. coli (APEC) may become opportunistic pathogens responsible for colibacillosis in birds. The inappropriate use of antibiotics can increase antimicrobial resistance, which may impact public health. This study aimed to detect the presence of the iroN virulence gene specifically in multidrug-resistant (MDR) E. coli isolates associated with APEC obtained from duck cloacal swabs collected at live markets in Surabaya. This study was designed as a descriptive cross-sectional survey, and the results are presented as prevalence proportions without inferential or correlation statistical testing, as iroN detection was performed exclusively on MDR isolates. Samples were collected from five live markets in Surabaya and cultured on MacConkey agar (MCA). E. coli was identified morphologically through Gram staining and biochemically using Triple Sugar Iron Agar (TSIA), Sulfide Indole Motility (SIM), Simmons Citrate Agar (SCA), indole, and methyl red–Voges–Proskauer (MR–VP) tests. Antimicrobial susceptibility testing was performed on all confirmed E. coli isolates (n = 120), and PCR analysis of the iroN gene was subsequently conducted only on MDR isolates (n = 14). The identification rate of E. coli in this study was 83.3% (120/144). Resistance to erythromycin was 81.7% (98/120), which likely reflects the intrinsic resistance of Gram-negative bacteria to macrolides rather than acquired antimicrobial resistance. This was followed by resistance to gentamicin at 19.2% (23/120), ciprofloxacin at 14.2% (17/120), aztreonam at 3.3% (4/120), and chloramphenicol at 2.5% (3/120). The prevalence of multidrug resistance (MDR) was 11.6% (14/120). Among these MDR isolates, 42.8% (6/14) were positive for the iroN virulence gene. No statistical association between MDR status and iroN carriage was assessed because non-MDR isolates were not screened for iroN. These findings provide baseline descriptive data on MDR and iroN-positive E. coli in traditional markets in Surabaya based on a limited panel of representative antimicrobial classes, without inferring specific resistance mechanisms such as extended-spectrum β-lactamase (ESBL) production.
Co-Authors Adi Sofyan Ansori, Muhammad Agrippina, Fidela Devina Dameanti, Fidi Nur Aini Eka Puji Endang Suprihati Ismayati, Maya Kendek, Irfan Alias Khairullah, Aswin Rafif Kurniasih, Dea Anita Ariani Kurniawan, Muhammad 'Ahdi Kurniawan, Muhammad ‘Ahdi Kusnoto Kusnoto, Kusnoto Lucia Tri Suwanti, Lucia Tri Moses, Ikechukwu Benjamin Mufasirin Mustofa Helmi Effendi Naseh, Naseh Poedji Hastutiek Putri, Mariana Febrilianti Resilinda Rehman, Saifur Riwu, Katty Hendriana Priscilia Riza Zainuddin Ahmad Sheila Marty Yanestria Sri Hidayati Tang, John Yew Huat Wibisono, Freshinta Jellia Yulianna Puspitasari Zainul Abidin