Tyagita Hartady
Department Of Basic Biomedical Science, Division Of Anatomy, Physiology And Cellular Biolog , Faculty Of Medicine, Padjadjaran University, Indonesia

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Demam tifoid sistemik yang disebabkan oleh Salmonella gallinarum pada ayam broiler: Bukti patologis dan mikrobiologis Azzahra, Tiara; Maharani, Devina Aisyah; Tsevaot, Amar Adonay; Hartady, Tyagita; Prayugo, Armanda Dwi; Windria, Sarasati; Krissanti, Ita; Satrio, Faisal Amri
ARSHI Veterinary Letters Vol. 9 No. 4 (2025): ARSHI Veterinary Letters - November 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.4.99-100

Abstract

Salmonella gallinarum, the causative agent of fowl typhoid, is a highly pathogenic bacterial disease associated with substantial mortality in poultry, predominantly affecting adult chickens and turkeys. This study aimed to describe the pathological lesions and microbiological characteristics of S. gallinarum infection in chickens with fowl typhoid. S. gallinarum is a gram-negative, rod-shaped, facultative anaerobic bacterium with an optimal growth temperature of 37 °C and the ability to survive across a broad pH range (4.0–9.0). Necropsy findings in affected chickens revealed interstitial pneumonia of the lungs, intestinal hyperemia with petechial hemorrhages, hepatomegaly, splenomegaly, and fatty degeneration of the cardiac tissue. Bacterial culture of intestinal swab samples on MacConkey agar demonstrated the growth of pale-yellow colonies consistent with S. gallinarum. Microscopic examination confirmed Gram-negative bacilli, while biochemical testing showed an alkaline/acid reaction, absence of gas and H₂S production, non-motility, and negative indole and urease reactions
Necropsy Findings, Isolation and Identification of Salmonella pullorum in Layer Chickens in Sumedang, Indonesia:  A Case Report Maharani, Briliant; Cyntia, Annisa Permata; Azra, Putri Zivana; Hartady, Tyagita; Windria, Sarasati; Prayugo, Armanda Dwi
Jurnal Veteriner Vol. 27 No. 1 (2026)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/

Abstract

Pullorum disease or Chalky feces, caused by Salmonella pullorum bacteria, is a highly contagious bacterial infection that significantly affects commercial poultry, particularly in developing countries. This study was aimed to report a case of pullorum disease in commercial layer chickens in Sumedang, West Java, Indonesia. The diagnostic approach included clinical examination based on clinical signs, followed by pathological and microbiological identification. Clinical signs observed were lethargy, white diarrhea (chalky feces) and decreased egg production. Necropsy findings revealed pathological changes including hepatomegaly, splenomegaly, caseous masses in the caecum and joints, oophoritis and white nodules in the heart. Additional anatomy pathological findings included tracheal hyperemia and malacia in the brain. Bacterial isolation and identification were conducted from internal organs using MacConkey Agar, followed by Gram staining and biochemical tests including Kligler’s Iron Agar (KIA), Motility Indole Urea (MIU) and Simmons’s Citrate Agar. The results confirmed the presence of S. pullorum bacteria, characterized by non-motile, Gram-negative bacilli. These findings demonstrate that S. pullorum can cause systemic infection affecting multiple organs. Based on pathological and microbiological examinations, it was concluded that the disease case in the layer chicken samples was caused by chronic infection of S. pullorum. This report highlights the importance of early diagnosis and improved biosecurity measures to prevent outbreaks, reduce mortality rates and minimize economic losses in Chicken farming.
Studi Kasus: Mikroskopis dan Makroskopis Penyakit Coryza pada Ayam Petelur di Peternakan Tanjungsari, Sumedang Setiady, Ashley Christa Darliani; Putri, Risti Yupiesta; Sofia, Syifa Rahma; Hartady, Tyagita; Windria, Sarasati; Prayugo, Armanda Dwi; Krissanti, Ita
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.106823

Abstract

Ayam petelur memainkan peran penting dalam industri perunggasan di Indonesia, seiring dengan meningkatnya permintaan konsumen. Namun, produktivitas mereka seringkali terancam oleh penyakit infeksius, seperti Infeksi Coryza, yaitu penyakit pernapasan akut yang disebabkan oleh Avibacterium paragallinarum. Studi ini meneliti temuan patologis mikroskopis dan makroskopis dari penyakit Coryza pada ayam petelur di Peternakan Tanjungsari, Sumedang, guna meningkatkan pemahaman terhadap ciri khas penyakit ini dan menentukan langkah pengendalian yang efektif. Hasil nekropsi pada ayam yang terinfeksi menunjukkan lesi khas, termasuk pembengkakan wajah, eksudasi sinus infraorbital, konjungtivitis, peradangan trakea dan paru-paru, serta atresia folikel. Secara mikroskopis, ditemukan adanya kondisi hemoragi dan kongesti pada trakea dan paru-paru. Metode diagnostik yang digunakan merupakan isolasi kultur bakteri dan uji biokimia. Isolasi bakteri pada media agar coklat mengkonfirmasi keberadaan A. paragallinarum, yang semakin memperkuat diagnosis.