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All Journal Jurnal Teknik Kimia USU AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biotropika Indonesian Journal of Pharmaceutical Science and Technology Prosiding Seminar Biologi Biota Biology, Medicine, & Natural Product Chemistry BIOMA International Journal of Applied Biology JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education Journal of Health Science and Prevention KLOROFIL: Jurnal Ilmu Biologi dan Terapan Al-Ard: Jurnal Teknik Lingkungan JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI PROFESI (Profesional Islam): Media Publikasi Penelitian KLOROFIL: Jurnal Ilmu Biologi dan Terapan Jurnal Jeumpa JOURNAL OF COMMUNITY ENGAGEMENT AND EMPOWERMENT Jurnal Teknologi Kesehatan Jurnal Teknik Kimia USU Engagement: Jurnal Pengabdian Kepada Masyarakat BIOTROPIC
Irul Hidayati
Universitas Islam Negeri Sunan Ampel Surabaya
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Potential of Black Garlic Nanoparticles as Skin Care Andiarna, Funsu; Hidayati, Irul; Agustina, Eva
Journal of Health Science and Prevention Vol. 8 No. 1 (2024): JHSP Vol 8 No 1 – 2024
Publisher : State Islamic University of Sunan Ampel

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/jhsp.v8i1.1188

Abstract

Mask is a beauty product that serves to treat and maintain the skin of the body and face. Black garlic contains antioxidant compounds so that it can counteract free radicals that cause premature aging, wrinkled and dull skin. The purpose of this study was to determine the potential of black garlic nanoparticles as skin care. The aim of this research is to develop a face mask product based on black garlic nanoparticles. The research method is by extracting black garlic by maceration and making nanoparticles of black garlic extract with a silver reducing agent. Black garlic extract nanoparticles were substituted for the manufacture of peel-off masks and tested for their characteristic. The results showed that the black garlic nanoparticle mask had a strong antioxidant activity of 21.168 ppm, the particle size is 59.47 nm and had good characteristics as a peel-off mask based on the viscosity, spreadability, drying test. Black garlic masks with nano-sized particles are able to penetrate the skin's pores more effectively, thus aiding in the repair of damaged skin cells.
Analisis Total Bakteri Coliform dan Identifikasi Escherichia coli pada Makanan dan Minuman di Kantin X Hidayati, Irul; Reni Ida Wati; Hanik Faizah
Al-Ard: Jurnal Teknik Lingkungan Vol. 8 No. 1 (2022): September
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/alard.v8i1.1488

Abstract

Food is one of the basic necessities of humans. It is important to pay attention to the quality, safety, hygiene and sanitation of food. Healthy food should contain nutrients, vitamins, and the other essential substances for our body need. The food should be safe for consumption, and free from contamination. Food contamination generally occurs due to coliform bacteria contamination that can cause foodborne disease. This study aimed to analyze the total coliform bacteria and identify Escherichia coli bacteria in food and beverage samples in the canteen X. The design of this study was descriptive research. Samples were obtained from four sellers in canteen X. From each seller, one sample of mixed rice and one sample of iced tea were obtained. Samples were analyzed using the MPN (Most Probable Number) method to determine the total coliform bacteria and EMB (Eosin Methylene Blue Agar) media to identify E. coli. The results showed that all food and beverage samples were tested positive for coliform and E. coli with MPN values ​​exceeding the threshold value. The lowest contamination in the food sample was found in the MA1 and MA2 (460 MPN/gram) and the highest contamination was found in the MA3 and MA4 (>1100 MPN/gram), while the lowest contamination in the beverage sample was found in the MI4 (1100 colonies/100 mL), and the highest contamination was found in the MI1, MI2, and MI3 (>2400 colonies/100 mL).
Effectiveness and Role of Symbiotic Culture of Bacteria and Yeast (SCOBY) Kombucha Gel on Incisional Wound Healing in Mice (Mus musculus) Nafisah, Raihana Frika; Agustina, Eva; Purnamasari, Risa; Andiarna, Funsu; Hidayati, Irul
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2025): October 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.11282

Abstract

Incision wounds are common occurrences in daily life, and appropriate wound management is essential to promote and accelerate the healing process. Wound care can be performed using topical preparations such as gels containing bioactive compounds. SCOBY kombucha is believed to contain bioactive compounds, such as compounds from green tea (especially polyphenols) and metabolites produced by microbes (organic acids, alcohols, vitamins, etc.) The aim of this research is to determine the effectiveness of SCOBY kombucha gel on the healing of incision wounds in mice (Mus musculus). The method used in this research is a Completely Randomized Design (CRD) with 8 treatment groups, including 2 control groups and 6 test groups. The concentrations of SCOBY kombucha gel used are 0%, 2%, 4%, 8%, 10%, and 12%. The SCOBY kombucha gel is applied twice daily until the wound closes. Observation of the incision wound is carried out by measuring the length of the wound that has not yet closed. Based on the conducted research, it was found that SCOBY kombucha gel is effective in healing incision wounds as evidenced by the reduction in wound size due to the bioactive compounds in SCOBY. The most significant wound healing effect was observed with the 12% SCOBY kombucha gel, as there was a significant difference compared to the negative control group, 0%, 2%, 4%, and 8% SCOBY kombucha gels.
The Effect of IBA and NAA on Root Induction of Tithonia diversifolia (Hemsl.) Ramadhani, Dwi Nova; Hidayati, Irul; Faizah, Hanik
Biotropika: Journal of Tropical Biology Vol. 13 No. 3 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.03.06

Abstract

Tithonia diversifolia (Hemsl.) is a plant that has a lot of potential for use in several sectors, so that large-scale and short-time propagation efforts are needed. Tissue culture is an appropriate technique for cultivating Tithonia diversifolia plants. The success of root induction in tissue culture is largely determined by the auxin growth regulator. This study aimed to determine the effect of IBA and NAA on root induction of T. diversifolia plants. The method was an experimental study with a Completely Randomised Design (CRD). Tithonia diversifolia plant node explants were planted into Murashige and Skoog (MS) media supplemented with growth regulators IBA (1 and 2 mg/L), NAA (1 and 2 mg/L), and a combination of IBA and NAA (1 mg/L + 1 mg/L; 1 mg/L + 2 mg/L; 2 mg/L + 1 mg/L; and 2 mg/L + 2 mg/L) and control group, consisting of explants cultured on a hormone-free medium which were then incubated for 4 weeks. The observation data were analysed statistically using the non-parametric Kruskal-Wallis test, followed by the Mann-Whitney test. The results showed that all parameters significantly affected auxins IBA and NAA in the root induction of T. diversifolia plants. The NAA 2 ppm treatment was the most effective in terms of root emergence time (3.67 DAP). Additionally, the combination of IBA and NAA was the optimal treatment in terms of root growth percentage (1.00%). The IBA 2 ppm + NAA 2 ppm treatment yielded the best results in terms of root number (10.17 strands). The NAA 1 ppm treatment yielded the best results in terms of root length (11.3 cm), root fresh weight (0.0967 g), and root dry weight (0.0144 g).
Application of Symbiotic Culture of Bacteria and Yeast Kombucha Gel on Incision and Burn Wounds in Mice (Mus musculus): Aplikasi Gel Symbiotic Culture of Bacteria dan Yeast Kombucha pada Luka Sayat dan Bakar Mencit (Mus musculus) Andiarna, Funsu; Hidayati, Irul; Agustina, Eva; Purnamasari, Risa
JRST (Jurnal Riset Sains dan Teknologi) Volume 10 No. 1, March 2026: JRST
Publisher : Universitas Muhammadiyah Purwokerto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/jrst.v10i1.24079

Abstract

SCOBY (Symbiotic Culture of Bacteria and Yeast) is a result of kombucha tea fermentation that contains active compounds, such as phenolic acids and flavonoids, which possess high antioxidant activity. However, the use of SCOBY is currently limited to serving as a starter culture for new batches of kombucha tea, necessitating innovation to enhance its other potential benefits. This study aims to evaluate the effectiveness of kombucha SCOBY gel in the healing of incised and burn wounds in mice (Mus musculus). The research methods included the preparation of kombucha tea, harvesting of SCOBY, formulation of gel, application of the gel to incised and burn wounds in mice, and observation of wound healing until closure. The results showed that kombucha SCOBY gel significantly accelerated the healing of both incised and burn wounds compared to the control group. Incised wounds fully healed by day 6, while burn wounds healed by day 7. This effectiveness is associated with the high total phenolic content (TPC) of the SCOBY gel, measured at 790.4 mg GAE/g extract, along with strong antioxidant activity of 12.42% (DPPH assay). Statistical analysis revealed a significant difference (p<0.05) in wound healing between the untreated and SCOBY gel-treated groups for both types of wounds. Therefore, kombucha SCOBY gel has potential as an effective natural topical wound-healing agent, particularly for mild to moderate wounds.   ABSTRAK (Bahasa Indonesia) SCOBY (Symbiotic Culture of Bacteria and Yeast) merupakan hasil fermentasi teh kombucha yang mengandung senyawa aktif, seperti asam fenolat dan flavonoid, yang memiliki aktivitas antioksidan tinggi. Namun penggunaan SCOBY hanya terbatas sebagai starter dalam pembuatan teh kombucha baru, sehingga diperlukan inovasi untuk meningkatkan manfaat lain dari SCOBY. Tujuan penelitian ini adalah untuk mengevaluasi efektivitas gel SCOBY kombucha dalam penyembuhan luka sayat dan luka bakar pada mencit (Mus musculus). Metode penelitian ini dimulai dari pembuatan teh kombucha, pemanenan SCOBY, pembuatan gel pengaplikasian pada luka sayat dan bakar pada mencit dan pengamatan penyembuhan luka sampai penutupan luka. Hasil penelitian menunjukkan bahwa gel SCOBY kombucha mempercepat penyembuhan luka sayat dan bakar secara signifikan dibandingkan kelompok kontrol. Luka sayat sembuh sempurna pada hari ke‑6, sedangkan luka bakar sembuh pada hari ke‑7. Efektivitas ini dikaitkan dengan tingginya kadar total fenolik (TPC) gel SCOBY, yaitu sebesar 790,4 mg GAE/g ekstrak, serta aktivitas antioksidan kuat sebesar 12,42% (uji DPPH). Hasil analisis statistik menyatakan pada luka sayat dan bakar terdapat perbedaan signifikan(p<0,05) antara kelompok tanpa perlakuan dan gel SCOBY. Dengan demikian, gel SCOBY kombucha berpotensi sebagai agen penyembuh luka topikal alami yang efektif, khususnya untuk luka ringan hingga sedang.
Analisis Kadar Glukosa, Hemoglobin, dan Kolesterol Mencit (Mus Musculus) Setelah Diinduksi Aspartam Esti Tyastirin; Risa Purnamasari; Irul Hidayati; Eva Agustina; Moch. Irfan Hadi; Nova Lusiana; Mei Lina Fitri Kumalasari
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.119-125

Abstract

Aspartame has a lower calorie rating than other sweeteners. for example, cyclamate, lactose, saccharin, fructose and maltosa. Although it has low calorie, aspartame have 60 times sweeter than sugar (sucrose). Induction of aspartame can cause elevated levels of blood glucose and damage to the pancreatic β cells of mice. The purpose of this study was to analyze the dosing of aspartame levels; 13 mg/Kg body weight, 39 mg/Kg and 78 mg/Kg and those effect to the levels of glucose, cholesterol and haemoglobin of female mice (Mus musculus). The methode of research laboratory experiments using a complete randomized design (RAL). This study was using 24 mice with 4 treatment groups. Mice were given aspartame during surgical treatment, mice were made to perform measurement of Hb levels, glucose levels and cholesterol levels of neonatal mice. Data analysis using the Kruskal Wallis because Gaussian data but not homogeneous. The results showed an increase in the levels of glucose and cholesterol levels, but statistically there is no difference in glucose levels (p = 0,087), Hb (p = 0,899) and cholesterol (p = 0,168) in mice (Mus musculus) who were given variations of the dose of aspartame.
Co-Authors AGUSTINA, EVA ANDYARINI, ESTI NOVI Arifin, Siti Hakimah Aprilia Garini Atiqoh Zummah Ayudya Fitri Arifa Azzahra, Fadila Kamilia Dedy Suprayogi Esti Tyastirin Eva Agustina Eva Agustina, Eva Faizah, Hanik Fiddaroini, Achmad Farchan Funsu Andiarna Gita Ika Safitri Hanik Faizah Hanik Faizah Iit Lusif Tsania Ika Mustika Irssa Intan Fatiha Ita Ainun Jariyah Linda Prasetyaning Widayanti Mei Lina Fitri Kumalasari Misbakhul Munir Moch. Irfan Hadi Muhammad Iqbal Pratama Nafisah, Raihana Frika Nirmala Fitria Firdhausi Nova Lusiana Rahmania Ramadhani, Dwi Nova Reni Ida Wati Reni Ida Wati RIA SAFITRI Risa Purnamasari Seftianingrum, Bella Shofiyya, Ainina Neihayatus Tania, Clarista Eka Vindi Fristy Kartika Wuri Handayani Yuanita Rachmawati