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T Allele of FOXO3 rs2802292 Increases CCL2 Concentration and Slightly Decreases TGF-β Concentration in Indonesian Elderly Nurfiyana, Wahyu; Iswanti, Febriana Catur; Hardiany, Novi Silvia
Molecular and Cellular Biomedical Sciences Vol 8, No 3 (2024)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v8i3.484

Background: Cellular senescence and the senescence-associated secretory phenotype (SASP) are pivotal factors influencing aging and age-related diseases. SASP secretes cytokines, chemokines, metalloproteinases, and growth factors that cause chronic inflammation. C-C ligand 2 (CCL2) and transforming growth factor-beta (TGF-β) are SASP markers secreted by senescent cells. This study investigated the relationship between the FOXO3 variant rs2802292 and SASP markers, focusing on CCL2 and TGF-β.Materials and methods: A cross-sectional study involving 72 elderly individuals from Jakarta was conducted. A sandwich enzyme-linked immunosorbent assay (ELISA)was used to quantify CCL2 and TGF-β concentrations. Random blood glucose, blood pressure, and FOXO3 rs2802292 genotyping data were obtained from a previous study. Differences in CCL2 and TGF-β concentrations between genotype groups were analyzed using one-way ANOVA and the Kruskal-Wallis test. Meanwhile, differences in CCL2 and TGF-β concentrations between allele groups were analyzed using the Mann-Whitney test.Results: The CCL2 and TGF-β concentrations of the subjects were 66.5 (10.58-190.9) pg/mL and 6,319 (2,379-13,846) pg/mL, respectively. There were significant differences in CCL2 concentrations among the FOXO3 rs2802292 genotypes (p=0.041). However, there were no significant differences in TGF-β concentrations among FOXO3 rs2802292 genotypes (p=0.955). Subjects with the G allele had significantly lower CCL2 concentrations compared with those with the T allele (p=0.033). TGF-β concentrations did not significantly differ between G and T alleles (p=0.771).Conclusion: CCL2 concentrations are associated with the FOXO3 variant rs2802292 in the elderly population. The T allele of FOXO3 rs2802292 increased CCL2 concentration and slightly decreased TGF-β concentration in elderly individuals.Keywords: aging, SASP, CCL2, TGF-β, SNP, FOXO3, rs2802292

Expression and correlation of endoglin, sEndoglin, and MMP-14 on preeclampsia placenta Iswanti, Febriana Catur; Mudjihartini, Ninik; Paramita, Reni; Purwosunu, Yuditiya; Prijanti, Ani Retno
Acta Biochimica Indonesiana Vol. 7 No. 2 (2024): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.179

Background: Hypertensive disorders, particularly preeclampsia, are major contributors to maternal mortality and neonatal morbidity. Angiogenic imbalance plays a critical role in placental ischemia, a hallmark of preeclampsia. Purpose: This study aimed to investigate the roles of endoglin, soluble endoglin (sEndoglin), and matrix metalloproteinase-14 (MMP-14) in the angiogenic imbalance observed in preeclampsia placentas compared to normal-term placentas. Method: A cross-sectional study was conducted using 68 placental samples: 34 from normal-term pregnancies and 34 from preeclampsia cases. Concentrations of endoglin, sEndoglin, and MMP-14 were measured using the sandwich ELISA method, and protein levels were determined using the Christian Warburg method. Data were analyzed using SPSS version 20. Results: The concentration of endoglin in preeclampsia placentas was slightly higher (1.37 [0.2–2.2] ng/μg protein) compared to normal placentas (1.12 [0.6–14.1] ng/μg protein), although the difference was not statistically significant. In contrast, sEndoglin (0.05 [0.0–0.01] ng/μg protein vs. 0.02 [0.0–0.3] ng/μg protein) and MMP-14 (0.14 [0.1–0.6] ng/μg protein vs. 0.11 [0.1–1.3] ng/μg protein) concentrations were significantly higher in preeclampsia placentas compared to normal placentas. All parameters showed a gradual decrease with advancing gestational age. sEndoglin and MMP-14 demonstrated a strong positive correlation (r = 0.658, p < 0.001), while endoglin and MMP-14 exhibited a moderate positive correlation (r = 0.554, p < 0.001). Conclusion: Endoglin, sEndoglin, and MMP-14 were differentially expressed in preeclampsia placentas, with sEndoglin and MMP-14 significantly elevated. These findings highlight their potential role in angiogenic imbalance and may provide insight into the pathophysiology of preeclampsia.

Innate and Adaptive Immune Components in Human Breast Milk and Their Role in Early-Life Immunity Salsabila, Hanum; Sukmawati, Dewi; Iswanti, Febriana Catur
Biomedical Journal of Indonesia Vol. 11 No. 3 (2025): Vol 11, No 3, 2025
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v11i3.272

Human breast milk is a uniquely complex biological fluid renowned for delivering essential nutrients to newborns. Beyond its nutritional value, it also contains important immunological components that play a fundamental role in infant health and development. This review explores the immunological characteristics of breast milk, highlighting its diverse array of immune cells and bioactive molecules, including secretory IgA, cytokines, and chemokines. These components actively contribute to the maturation of the infant’s immune system, strengthen defenses against infections, and facilitate the development of a balanced gut microbiome. This discussion also explores how the immune components in breast milk function to benefit infants, examining their protective mechanisms and developmental impacts. Additionally, it addresses how storage conditions—such as freezing, refrigeration, or pasteurization—may alter the integrity and effectiveness of these vital immune factors. Furthermore, the influence of external environmental factors, including maternal diet, stress, and exposure to pollutants, is considered for their potential effects on the immunological quality of breast milk. For this literature review, relevant studies were systematically searched across multiple academic databases, including PubMed, ScienceDirect, Google Scholar, ResearchGate, and Elsevier. The search was limited to publications from the past ten years. Emerging research underscores the adaptive nature of breast milk and its profound impact on early-life immunity, offering valuable insights for optimizing infant feeding practices and potential clinical applications.

Evasion of the Immune System by Mycobacterium tuberculosis: A Special Review on Macrophages Handayani, Kurnia Maidarmi; Iswanti, Febriana Catur
Health and Medical Journal Vol 6, No 2 (2024): HEME May 2024
Publisher : Universitas Baiturrahmah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33854/heme.v6i2.1452

Mycobacterium tuberculosis, the bacterium that caused tuberculosis, is estimated to affect 10 million people worldwide in 2019. This bacterium is an intracellular pathogen that is spread through the inhalation of bacterial aerosol particles. The innate immune system in the lungs is prepared to phagocytize these bacteria, particularly macrophages, dendritic cells, monocytes, and neutrophils. M. tuberculosis can evade attacks by the host immune system and has developed strategies to infect successfully, especially macrophages. This intracellular bacterium can inhibit phagolysosome fusion, which is associated with lipoarabinomannan (LAM) in the bacterial cell wall. M. tuberculosis also can persist in phagolysosomes by inhibiting acidification and also inhibiting the action of NOX2 from producing ROS. This ability also allows these bacteria to avoid autophagy within macrophages. Knowledge of the power of these bacteria to manipulate and evade the immune system, especially macrophages, is beneficial in developing medicines and vaccines in the future.

Senescence-Induced Atherosclerosis: The Potency of Senolytic Therapy Ratih, Udani Sari; Iswanti, Febriana Catur
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 8 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i8.1036

The aging process is an inevitable occurrence that involves physiological changes at the cellular level. The presence of intrinsic and extrinsic stressors can cause cellular damage, leading to senescence and premature aging. Senescent cells undergo activation of the p53/p21 and p16INK4a pathways, induce cell cycle arrest, increased expression of senescence-associated beta-galactosidase (SA-β-Gal), and secretion of SASP (senescence-associated secretory phenotype), leading to "inflamm-aging" or chronic inflammation associated with senescence. These premature aging and “inflamm-aging” accelerates the occurrence of age-related diseases, one of which is atherosclerosis. The relationship between premature aging, senescence, and atherosclerosis has been a focus of research on pathogenesis, prevention, and therapy. Recent research has emphasized the crucial role of senolytics, compounds or agents capable of eliminating senescent cells, in inhibiting the progression of atherosclerosis and slowing down premature aging. Obtaining a more comprehensive understanding of the processes and effectiveness of senolytics in premature aging and atherosclerosis should facilitate the development of more potent medicines to mitigate side effects in the management of cardiovascular disease and extend longevity.

The Role of High Sensitivity C-Reactive Protein as an Inflammation Predictor in Cardiovascular Diseases Cici Nuriah; Iswanti, Febriana Catur; Ariel Pradipta
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 9 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i9.1075

Inflammation can be measured by analyzing the levels of inflammatory markers, such as High Sensitivity C-reactive Protein (hs-CRP). This protein plays a crucial role as a strong independent predictor indicating the risk of cardiovascular. The hs-CRP can measure the levels of C-reactive protein with high sensitivity, making it a highly responsive marker to acute-phase inflammation. The presence of hs-CRP serves as an inflammation indicator that can predict the potential occurrence of heart attacks, strokes, peripheral artery diseases, and sudden death due to Acute Coronary Syndrome (ACS). Produced by the liver in response to inflammation, hs-CRP is nonspecific and can elevate various types of inflammation, including infections and chronic inflammatory conditions in cardiovascular. This study is a literature review using secondary data from related research. The hs-CRP has a very high sensitivity and specificity level compared to CRP as an inflammation marker. Therefore, hs-CRP serves not only as a diagnostic tool but also as a strong predictor of inflammation, capable of predicting cardiovascular risk.

Macrophage modulation in activation process induced immune thrombocytopenia Mahdaleny, Mahdaleny; Bustami, Arleni; Iswanti, Febriana Catur
Universa Medicina Vol. 43 No. 1 (2024)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2024.v43.76-87

The immune system operates like an orchestra that harmoniously maintains the homeostasis balance while protecting from external or internal pathogens attack. Inflammation is one of the key critical immune defenses to eradicate pathogens and encourage tissue repair and recovery by activating the host’s immune and non-immune cells. As a part of the immune response during inflammation, blood platelets serve various functions; however, their activation and involvement in inflammation can also contribute to pathological conditions, such as thrombosis, which results in myocardial infarction, stroke, and venous thromboembolism. Activated platelets can mobilize and release intracellular granules (alpha and dense granules), which include secondary mediators like chemokine PF4/CXCL4. In contrast to most other chemokines, PF4 participates in several long-term regulatory processes, such as cell differentiation, survival, and proliferation. However, recent findings suggest that PF4 is also responsible for modulating macrophage polarization, which can substantially impact the development of induced immune thrombotic thrombocytopenia. This review aims to explain how PF4 induced vascular problems by modulation of macrophage development during immunological thrombocytopenia. A literature search using the keywords PF4, CXCL4, macrophage M4, platelet macrophage M4, and induced immune thrombocytopenia was done using the following databases: Google Scholar, ProQuest, ScienceDirect, and Scopus for articles published from 2000 to 2023. The literature study was done to find the connection between platelet activation, macrophage modulation, and vascular problems such as atherosclerosis and thromboembolism in induced immune thrombotic thrombocytopenia. Several recent studies on PF4, macrophage modulation, and vaccine-induced thrombotic thrombocytopenia were carefully reviewed. This review concludes that macrophage polarization modulation is promising in managing vascular problems in patients with induced immune thrombotic thrombocytopenia.

Freediving, Hypoxia, and Inflammation: Physiological Adaptations and Interactions between HIF and NF-κB Amalina Fakhriah; Novi Silvia Hardiany; Iswanti, Febriana Catur
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 7 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i7.1026

Freediving presents a unique set of physiological challenges and adaptations, making it a subject of interest for researchers studying the effects of extreme environmental conditions on the human body. There is a complex interplay between freediving, hypoxia, immune responses, and inflammation, shedding light on the physiological effects of freediving on the human body. This article describes how HIF and NF-κB interact during hypoxia and inflammation, including their synergistic effects and signaling pathways. The regulatory loop involving these transcription factors is highlighted, providing insight into their linked roles in modulating the cellular response to hypoxia and inflammation.

Senescence-Induced Atherosclerosis: The Potency of Senolytic Therapy Ratih, Udani Sari; Iswanti, Febriana Catur
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 8 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i8.1036

The aging process is an inevitable occurrence that involves physiological changes at the cellular level. The presence of intrinsic and extrinsic stressors can cause cellular damage, leading to senescence and premature aging. Senescent cells undergo activation of the p53/p21 and p16INK4a pathways, induce cell cycle arrest, increased expression of senescence-associated beta-galactosidase (SA-β-Gal), and secretion of SASP (senescence-associated secretory phenotype), leading to "inflamm-aging" or chronic inflammation associated with senescence. These premature aging and “inflamm-aging” accelerates the occurrence of age-related diseases, one of which is atherosclerosis. The relationship between premature aging, senescence, and atherosclerosis has been a focus of research on pathogenesis, prevention, and therapy. Recent research has emphasized the crucial role of senolytics, compounds or agents capable of eliminating senescent cells, in inhibiting the progression of atherosclerosis and slowing down premature aging. Obtaining a more comprehensive understanding of the processes and effectiveness of senolytics in premature aging and atherosclerosis should facilitate the development of more potent medicines to mitigate side effects in the management of cardiovascular disease and extend longevity.

The Role of High Sensitivity C-Reactive Protein as an Inflammation Predictor in Cardiovascular Diseases Cici Nuriah; Iswanti, Febriana Catur; Ariel Pradipta
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 8 No. 9 (2024): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v8i9.1075

Inflammation can be measured by analyzing the levels of inflammatory markers, such as High Sensitivity C-reactive Protein (hs-CRP). This protein plays a crucial role as a strong independent predictor indicating the risk of cardiovascular. The hs-CRP can measure the levels of C-reactive protein with high sensitivity, making it a highly responsive marker to acute-phase inflammation. The presence of hs-CRP serves as an inflammation indicator that can predict the potential occurrence of heart attacks, strokes, peripheral artery diseases, and sudden death due to Acute Coronary Syndrome (ACS). Produced by the liver in response to inflammation, hs-CRP is nonspecific and can elevate various types of inflammation, including infections and chronic inflammatory conditions in cardiovascular. This study is a literature review using secondary data from related research. The hs-CRP has a very high sensitivity and specificity level compared to CRP as an inflammation marker. Therefore, hs-CRP serves not only as a diagnostic tool but also as a strong predictor of inflammation, capable of predicting cardiovascular risk.

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