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INDONESIA
BIOTROPIC The Journal of Tropical Biology
Published by Universitas Islam Negeri Sunan Ampel
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 94 Documents
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Identifikasi Bakteri Toleran terhadap Logam Berat Pb yang Diisolasi dari Air dan Sedimen di Sungai Porong, Sidoarjo Nur Rokhmatul Lailiya; Misbakhul Munir; Esti Tyastirin; Eko Teguh Pribadi; Hanik Faizah
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Porong River is an active river located in the Sidoarjo region, East Java which has been polluted by the Lapindo mudflow containing various chemical compounds, one of which is lead (Pb) heavy metal. Isolation of bacteria in the environment contaminated with Pb allows bacteria that have the potential as bioremediation agents to grow. The purpose of this study was to determine the content of heavy metal Pb and isolate and identify lead (Pb) tolerant bacteria in water and sediment at Porong river, Sidoarjo. Sampling of water and sediment was carried out at 2 sampling points based on the presence of Lapindo mud waste flow. Analysis of the heavy metal content of Pb was carried out using the Atomic Absorption Spectrophotometer (AAS). Bacterial isolation was carried out on NA media containing 10 ppm Pb of heavy metal and incubated for 24 hours at 300C. Bacterial isolates were identified based on the macroscopic and microscopic properties and biochemical tests. The results of the heavy metal content of Pb at sampling point 1 in water was 0.81 ppm, and in sediment was 0.98 ppm while at sampling point 2 in water was 2.93 ppm, and in sediment was 3.88 ppm. The results of bacterial identification obtained as many as 6 bacterial isolates belonging to 3 genera of bacteria including Bacillus, Acinetobacter and Pseudomonas.
Analisis Kuantitatif Kandungan Antioksidan dan Aktivitas Sitotoksik dari Ekstrak Anastatica hierochuntica L Nova Lusiana; Risa Purnamasari; Eva Agustina; Nurul Ilmi Faidah; Azlinda Mitha Agustin; Muhammad Falikhul M
Biotropic : The Journal of Tropical Biology Vol. 6 No. 2 (2022): Biotropic, Volume 6 Nomor 2, 2022
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

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Abstract

Antioxidants are compounds that can counteract free radicals in the body due to the presence of active compounds such as flavonoids and phenolics. These active compounds can be cytotoxic, ie, compounds capable of inhibiting and stopping the growth of cancer cells by using toxicity test by BSLT (Brine Shrimp Lethality Test) method. This study aims to determine the antioxidant activity by DPPH (Dipheny Pycryl Hydrazil) method and toxicity test by BSLT method of Anastatica hierochuntica L extract. A. hierochuntica L extraction were done by maceration method with methanol solvent. The content of A. hierochuntica L extract compounds was done by phytochemical test to know flavonoid compounds, triterpenoid and sterol. Meanwhile, alkaloid and saponin and liquid chromatography to see flavonoid compounds of quersetin. Furthermore, the antioxidant activity test was carry out by the DPPH method and by BSLT method to observed the mortality of shrimp larvae. A. hierochuntica extract has antioxidant activity with IC50 value 77,23 μg / ml and toxicity value of LC50 44,97. A.hierochuntica L extracts potentially have antioxidant and cytotoxic properties.
Isolasi dan Karakterisasi Mikroorganisme Endofit Akar Ginseng Jawa (Talinum paniculatum Gaertn.) Yang Diberi Perlakuan Perbedaan Ketersedian Air Visi Tinta Manik; Intan Nurcahya; Suhardjadinata; Susi Setiaramdani
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1694

Abstract

A medicinal plant known as Java ginseng (Talinum paniculatum Gaertn.) may thrive in a variety of water availability situations. The quantity of endophytic bacteria a plant contains affects its capacity to survive in stressful environments. The objective of this study was to identify and separate endophytic bacteria from Javanese ginseng roots that had undergone various water availability treatments. A description method was utilized, in which isolated isolates were given morphological descriptions, assessed for similarity indexes, and then categorized. Endophytic microbe abundance and diversity varied according to this study, but plants treated with water scarcity displayed higher abundance and diversity. While endophytes of plant origin treated with 60% field capacity (FC) had the maximum diversity, root samples treated with 80% (FC) had the highest microbial abundance. Ten species groupings were created from the 42 isolated isolates based on a coefficient value of > 70%.
Pengaruh Pemberian Infusa Albedo Semangka terhadap Perubahan Ukuran Diameter Pulau Langerhans Pankreas Tikus Jantan (Rattus norvegicus) Diabetes Mellitus Widi Pangestu Wilujeng; Nurul Mahmudati; Ladiya Fitri Ma’rufah; Ninik Fadhillah; Fatmi Indah Hati; Gebby Agnessya Esa Oktavia; Aurira Thrisna Dwi Aprianti; Berlian Rustantina; Nazil Dwi Rahayuningtyas; Azizaturrohmah; Farah Annisa Nurbani; Hanik Faizah; Alfiah Hayati
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1715

Abstract

Diabetes Mellitus condition (DM) correlates with the damage that occur in pancreas especially in Langerhans islets, one of the damage indicators is diameter changes of Langerhans islets that getting smaller compare with the normal sizes. The use of natural ingredients to control diabetes mellitus can be done by infusing sweet beauty watermelon albedo which can be found abundant in traditional market. Albedo layer of watermelon contain antioxidant compounds that can help to regenerate Langerhans islets of pancreas. This study purpose was to know the effect of giving watermelon’s infusion albedo to diameter of Langerhans islets in diabetes mellitus rats (Rattus norvegicus), and analyze the potential process occurred based on the result as information source for other research. This study used 25 male rats (Rattus norvegicus) as animal model with 5 groups treatment which were normal group, positive group as control (DM without treatment), A group (DM+1000 mg/kgBB watermelon’s infusions albedo), B group (DM+1500 mg/kgBB watermelon’s infusions albedo), and C group (DM+2000 mg/kgBB watermelon’s infusions albedo). After all the treatments, the rats were anesthetized and dissected and then their pancreas was taken. The pancreas was processed into histological slides by fixing the pancreas in a fixative solution (10% formalin) then dehydrated in a series of graded ethanol and embedded in paraffin wax, cut at a thickness of 4 µm, and finally stained with hematoxylin-eosin dye for microscopic observation, according to the stages Histopathological analysis of the gonads was examined using standard methods. Data was collected by measuring the size of Langerhans islets with microscope Olympus CX33  and connected to the raster image application.  Data results from this study indicate that watermelon’s infusion albedo had effect to increase the diameter of Langerhans islets of rat with diabetes mellitus
Karakteristik Bakteri Pendegradasi Bahan Pangan Asal Saluran Pencernaan Kecoa Amerika (Periplaneta americana) dari Pasar Tradisional Dwiana Muflihah Yulianti; Arif Rahman Hikam; Trisnowati Budi Ambarningrum; Taruna Dwi Satwika; Dyah Fitri Kusharyati; Hilmiyah Al'alawiyah
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1662

Abstract

American cockroach (Periplaneta americana) is one of the most common pests found in houses, apartments, and public facilities such as markets. As vector insects, cockroaches have symbiosis with microorganisms. Some of these microorganisms can live in cockroaches' cuticles and digestive tracts. Besides being able to pose a danger to human health, the microbes in cockroach bodies also have the potential to cause food damage. This study aimed to determine the diversity of microorganisms originating from the digestive tract of the American cockroach and their potential to cause food spoilage. The research was conducted in several stages: sampling cockroaches at a traditional market, isolating bacteria from the digestive tract of cockroaches, qualitatively testing amylolytic, proteolytic, and lipolytic potentials, and characterizing bacterial isolate by morphologically and biochemically character. The results showed that there were 26 isolates of bacteria isolated from the digestive tract of American cockroaches from the market, 62.96% of the isolates were found to have the ability to degrade protein, 37.04% of the isolates had the ability to degrade starch, and 40.74% of the total isolates can degrade fat. These isolates had various morphological and biochemical characteristics. Bacterial isolates derived from American cockroaches from traditional markets have the potential to degrade food.
Keragaman Morfologi Bakteri Nitrifikasi Asal Kompos Kotoran Domba pada Peternakan Domba dengan Sistem Bedding Sri Martina Wiraswati; Hendro Pramono; Oedjijono; Dini Ryandini; Dyah Fitri Kusharyati; Meyta Pratiwi; Taruna Dwi Satwika; Dwiana Muflihah Yulianti; Saefuddin Aziz; Daniel Joko Wahyono; Rizal Khoirun Alfisah; Yuriza Eshananda; Budi Rustomo; Afifah Mariana
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1695

Abstract

The application of compost bedding system on sheep farm is a technology to minimize the negative impact of sheep manure waste toward environment. The microorganisms which inhabit the compost bedding, especially nitrifying bacteria are expected to have significant role in odor emission reduction which become environmental problem around the sheep farm. This study aims to analyze the abundance and diversity of nitrifying bacteria of compost bedding obtained from sheep farm which have important role in reducing odor emission of ammonia. The 12 days, 1 and 1,5 months old of compost bedding samples were obtained from sheepfold, while 3 and 4 months old of compost samples were obtained from compost pile. Furthermore, nitrifying bacteria were isolated from compost samples by pour plate method using specific media for Nitrosomonas and Nitrobacter. The results indicated that compost bedding samples with different composting time duration displayed different abundance and diversity of nitrifying bacteria. The current study was successfully isolated 39 and 47 nitrifying bacterial isolates using specific media for Nitrobacter and Nitrosomonas, respectively. The highest diversity of nitrifying bacteria was gained from 12 days old compost bedding sample. Nitrifying bacterial isolates from compost bedding samples have significant role in odor emission declining as well as manure composting at sheep farm. The obtained bacterial isolates are also potentially to develop as bio-activator for compost bedding.
Gambaran Kualitas Lingkungan Sungai Cimandiri Akibat Cemaran Merkuri di Wilayah Penambangan Emas Skala Kecil (PESK) Kabupaten Sukabumi Izza Hananingtyas
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1707

Abstract

In Indonesia, ASGM has increased. This results in an increased risk of mercury pollution in the environment (Minamata Initial Assessment Report, 2019).  The amalgamation process for ASGM in Sukabumi, it used mercury as basic material for amalgamation process. It can increase to negative impacts on the environment and humans. This study aims to describe the impact of mercury contamination on the environmental quality of the Cimandiri River around ASGM in Sukabumi District. This research was conducted using a laboratory test method with reference to PP No. 22 at 2021 and SNI 7387 at   2009. The results of the tests found that the highest mercury exposure occurred in aquatic biota (catfish, snakehead and eel) which exceeded the quality standard, although the media water and sediment does not exceed the quality standard. This indicates that the quality of the Cimandiri River is unsafe for biota due to mercury contamination in local biota around ASGM. Therefore, there is a need for further studies on the safety of local food products from the Cimandiri River.
Optimization of RNA Extraction from Aedes aegypti and Aedes albopictus Saiku Rokhim; Ninik Fadhillah; Radinal Kautsar; Humayra Qurrata Aini; Yuanita Rachmawati
Biotropic : The Journal of Tropical Biology Vol. 7 No. 1 (2023): Biotropic, Volume 7 Nomor 1, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i1.1781

Abstract

RNA extraction is the critical initial stage in analyzing certain gene expressions, further analysis using Real Time PCR technology, and performing virus detection. However, the process of extracting RNA is often hampered by the risk of contamination, resulting in low concentrations of RNA and low purity of RNA. This is often an obstacle in extracting mosquito RNA especially detecting Dengue Virus (Den-V). Dengue virus (Den-V) can cause dangerous diseases in humans such as Dengue Fever (DHF) which is transmitted through the bites of Aedes aegypti and Aedes albopictus mosquitoes. This study aims to find out the effective steps for extracting RNA from Aedes aegypti and Aedes albopictus mosquitoes. The method being compared is a commercial RNA extraction kit with modification (addition of β-mercaptoethanol) and without modification. The results showed that the best DNA concentration and purity were obtained in mosquito samples from modified process. The purity ratio of RNA extracted without modification was 1.971 (0.021 ± 0.800) while with modification it was 2.003 (0.011 ± 0.112). Aedes aegypti had a better average concentration of 7.146 µg/ml for unmodified RNA and 7.613 µg/ml for modified RNA. This research is expected to be a reference for further studies on viruses in Aedes aegypti and Aedes albopictus.
Analisis Mikrobiologi Dengke Naniura Ikan Mas (Cyprinus carpio L) Terhadap Pengaruh Konsentrasi Andaliman (Zanthoxylum acanthopodium DC) fadhliani; Yesika Rumondang Sitorus; Zidni Ilman Navia
Biotropic : The Journal of Tropical Biology Vol. 7 No. 2 (2023): Biotropic, Volume 7 Nomor 2, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i2.1869

Abstract

Dengke naniura is a traditional fermented food made from carp which is often consumed in the traditional ceremonies of the Batak people. This study aims to determine the effect of the concentration of andaliman (Zanthoxylum acanthopodium DC) the best concentration of andaliman (Zanthoxylum acanthopodium DC) on microbes in dengke naniura. The method used in this research is an experimental method. The experimental design that will be used is a non-factorial Completely Randomized Design (CRD), with 4 variations of andaliman concentrations of 0 g (A0), 25 g (A1), 27.5 g (A2), 30 ( A3). Dengke naniura’s microbial testing was carried out at the Samudra University Laboratory. The best concentration after addition andaliman to the microbial colonies that appeared after incubation was at the  A3  (30 g) concentration. The number of microbes that can be counted on the addition 30 g andaliman is 3.66 x 104 colonies/g had met the standardization of SNI for food maximum 5.0 x 105 colonies/g.
Pemanfaatan Limbah Organik Tulang Ayam dan Sisa Nasi Sebagai Pakan Larva Lalat Tentara Hitam (Hermetia illucens L.) Eunike Dian Swastoko; Kukuh Madyaningrana; Krismono Krismono
Biotropic : The Journal of Tropical Biology Vol. 7 No. 2 (2023): Biotropic, Volume 7 Nomor 2, 2023
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v7i2.1876

Abstract

Organic waste originating from restaurants brings problems to the environment because it can cause bad smells and environmental pollution. To reduce this pollution, organic waste can be decomposed and used as feed for black soldier fly (BSF) larvae can be useful as fish or poultry feed with high nutrition. Therefore, this research aimed to determine and study the feeding of organic waste, namely leftover rice and chicken bones, on the growth of BSF larvae. The resesearch was conducted using a completely randomized design (CRD) with 3 feeding treatments consist of leftover rice, chicken bones, and combination of leftover rice and chicken bones; and control treatment used T-51 pig feed. All teratments used triplicates. Data obtained from this study consist of BSF larvae growth parameter for 12 days, and proximate analysis of the feed and BSF larvae. Results showed that larvae fed with a combination of leftover rice and chicken bones was the best treatment to support the groeth of BSF larvae as shown by values of waste reduction index (WRI) 0,034 g/day, substrate reduction 51%, and 98 % of BSF larvae survival rate 98% in 12 days.

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