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Jurnal Biologi Tropis
Published by Universitas Mataram
ISSN : 14119587     EISSN : 25497863     DOI : -
Jurnal Biologi Tropis (ISSN Cetak 1411-9587 dan ISSN Online 2549-7863) diterbitkan mulai tahun 2000 dengan frekuensi 2 kali setahun oleh Program Studi Pendidikan Biologi PMIPA FKIP Universitas Mataram, berisi hasil penelitian dan ulasan Ilmiah dalam bidang Biologi Sains.
Arjuna Subject : -
Articles 2,695 Documents
Overview of MYH9 Gene Polymorphism SNP RS3752462 in Chronic Kidney Disease (CKD) Patients with Diabetic Nephropathy Maulana, Ahmad Akmal; Qurrohman, Muhammad Taufiq
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11712

Abstract

Diabetic nephropathy is a major cause of chronic kidney disease and is influenced by both environmental and genetic factors. One of the genes implicated in the pathogenesis of diabetic nephropathy is MYH9, particularly the Single Nucleotide Polymorphism (SNP) rs3752462. This study aimed to describe the polymorphism of the MYH9 gene SNP rs3752462 in patients with chronic kidney disease associated with diabetic nephropathy. A descriptive study design was applied, involving five patients who met the inclusion criteria. Blood samples were analyzed using the Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP) method with the RsaI restriction enzyme. The Results showed that the DNA band patterns observed in all samples did not form consistent combinations and did not correspond to the expected genotype classification standards. In addition, extra bands outside the target fragment sizes and variations in band intensity among samples were observed. Based on these findings, the resulting band patterns could not be reliably used to accurately determine genotypes, and therefore did not adequately represent the polymorphism of the MYH9 gene SNP rs3752462 in the studied samples. Accordingly, further optimization of the PCR-RFLP method is required, along with validation using more accurate approaches, such as DNA sequencing, and a larger sample size to obtain more reliable results.
Fermentation of Banana Stem and Indigofera sp. Leaves Using MA-11 with Different Fermentation Durations on Nutritional Quality Herlina, Febriana Puja; Mulyono, Ali Mursyid Wahyu; Husein, Muhammad
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11726

Abstract

Banana stems are an abundant agricultural by-product; however, their utilization as animal feed remains limited due to their high fiber and low crude protein contents. Improving their nutritional quality can be achieved through fermentation combined with the addition of protein sources and bioactivators. This study aimed to evaluate the effect of fermentation duration on the nutritional quality of a mixture of banana stems and Indigofera sp. leaves fermented using Microbacter Alfaafa (MA-11). The experiment was conducted for 12 days using a Completely Randomized Design (CRD) with three fermentation periods: 0 days (P0), 6 days (P1), and 12 days (P2), each with four replications. The observed parameters included crude protein, crude fiber, and dry matter biomass weight. Data were analyzed using analysis of variance (ANOVA), followed by Duncan’s Multiple Range Test at a 5% significance level. The results indicated that fermentation duration had a significant effect (P<0.05) on crude protein content, with the 12-day treatment producing the highest value (15.14%). In contrast, fermentation duration had no significant effect (P>0.05) on crude fiber content and dry matter biomass weight. In conclusion, 12 days of fermentation with MA-11 increased crude protein content but was not effective in significantly reducing crude fiber and dry matter biomass weight.
Nutritional Quality of a Mixture of Indigofera sp. Leaves and Banana Steams Fermented Using Ma-11 Inoculant at Different Doses Purnamasari, Yunita; Mulyono, Ali Mursyid Wahyu; Husein, Muhammad
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11727

Abstract

Feed availability is one of the main constraints in livestock production, especially in tropical regions where supply is highly influenced by seasonal changes. Banana stems have potential as livestock feed due to their abundant availability; however, they contain low protein and high crude fiber, which requires processing to improve their nutritional quality. One of the methods that can be applied is fermentation using a bioactivator. This study aimed to evaluate the effect of different doses of Microbacter Alfaafa-11 (MA-11) inoculant on the nutritional quality of fermented banana stem and Indigofera sp. leaves. The study utilized a Completely Randomized Design (CRD) featuring three dose levels (0%; 0.05%; and 0.1%) and included four replicates over a period of 12 days of anaerobic fermentation. The measured variables were crude protein, crude fiber, and the weight of dry matter biomass. The data were examined using analysis of variance (ANOVA), followed by Duncan’s Multiple Range Test at a significance threshold of 5%. The findings indicated that the addition of MA-11 notably raised the crude protein level (P<0.05), with the highest value recorded at the 0.1% dose; however, it did not significantly affect (P>0.05) the crude fiber or the dry matter biomass weight. These findings indicate that MA-11 is more effective in improving protein content than in reducing crude fiber or modifying dry matter biomass weight.
Zinc (Zn) and Iron (Fe) Residues in Cattle Liver: A Comparative Study in Mining and Non-Mining Zones Wulandari, Sitti Aisyah May; Jannah, Raodatul
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11728

Abstract

Laterite nickel mining activities in Southeast Sulawesi significantly contribute to environmental contamination, potentially leading to the bioaccumulation of heavy metals in free-grazing livestock. The liver, functioning as a primary metabolic organ, serves as a sensitive bioindicator for heavy metal exposure. This study aimed to analyze and compare the concentrations of essential heavy metals, Zinc (Zn) and Iron (Fe), in the liver of cattle reared in mining circum-areas (Sopura and Oko-oko) versus non-mining control areas (Toari and Watubangga). Liver samples were collected from 12 cattle (n = 3 per location). The samples were prepared using wet digestion and analyzed using Atomic Absorption Spectrophotometry (AAS). Data were analyzed using Independent Sample T-test and One-Way ANOVA followed by Tukey HSD post-hoc test. The results showed a significant difference (p < 0.05) in metal concentrations between mining and non-mining areas. The mining area of Oko-oko exhibited an extreme accumulation of Iron (Fe), reaching 1,803.40 ± 48.64 mg/kg, which is significantly higher than the control area of Watubangga (47.69 ± 1.94 mg/kg). Zinc (Zn) levels were also significantly higher in mining areas (21.88–24.22 mg/kg) compared to non-mining areas (2.97–8.30 mg/kg). There is a strong correlation between mining activities and the bioaccumulation of Zn and Fe in bovine liver. The extreme iron levels detected in the mining zone suggest a potential health risk for consumption, necessitating the implementation of depuration strategies and stricter food safety monitoring in the affected regions.
Carbon Stock Potential of Mangrove Species in South Lombok Coastal Ecotourism Area Luthfiyannisa, Luthfiyannisa; Al Idrus, Agil; Syukur, Abdul
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11729

Abstract

Data on carbon stocks in  mangroves within coastal ecotourism areas remain limited, despite their high potential as tropical blue carbon sinks. This study evaluates the carbon stock potential of mangrove species at Bale Mangrove Ecotourism Site using line transect-quadrat plot methods and IPCC guidelines, incorporating allometric biomass analysis (V = π(d/2)²th with genus-specific wood density). Key findings reveal total aboveground biomass of 22.95 ton/ha and carbon stock potential of 11.48 ton C/ha (equivalent to 42.12 ton CO₂/ha), dominated by Rhizophora mucronata (3.96 ton C/ha) due to larger stand size and high wood density (0.92 g/cm³), while Avicennia marina was lowest (0.06 ton C/ha) owing to minimal abundance. These inter-species differences reflect early  dynamics where pioneer Rhizophora excels in initial biomass accumulation. Overall, results confirm mangrove restoration's role in IPCC-based carbon inventories, with implications for sustainable ecotourism management to maximize long-term blue carbon capacity
Pathogenic Bacterial Genes Resistant to Antibiotics in Chickens: Systematic Literature Review Rosyunita, Rosyunita; Wardoyo, Eustachius Hagni; Hasbi, Nurmi; Rahim, Adelia Riezka
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11732

Abstract

Chicken meat and eggs are widely consumed sources of protein, increasing consumer demand and leading to the use of antibiotics as prophylaxis and therapeutics. The purpose of this narrative review is to describe the colonization of chicken parts and organs and summarize the resistance genes in chickens reported from 2015 to 2026. The method used a systematic literature review with a narrative review. This review focuses on four main bacteria: Escherichia coli, Salmonella spp., Campylobacter spp., and Enterococcus spp. The results showed that almost all chicken organs were colonized by bacteria carrying various antibiotic resistance genes. These resistance genes included fluoroquinolones (gyrA, gyrB), tetracyclines (tetA, tetB, tetO, tetM, tetL), β-lactams (blaTEM, blaSHV, blaCTX-M, blaCMY, blaOXA-61), aminoglycosides (aac, aad, aph, strA/B), and sulfonamides (sul1, sul2, sul3). The presence of multidrug efflux pump genes (cmeB, cmeG) in Campylobacter spp. further highlights the increasing prevalence of multidrug resistance. Colonization of antibiotic-resistant pathogenic bacteria with resistance genes has been found in vital organs of chickens. These findings highlight the importance of molecular surveillance and integrated One Health strategies to reduce the spread of AMR.
Genetic Research Trends in Caffeine Biosynthesis of Robusta Coffee (Coffea canephora) Permatasari, Nindy; Sari, Sismita; Nurmayanti, Sri; Fauziah, Lu'lu' Kholidah; Sari, Resti Puspa Kartika; Hardani, Maisuri; Aliyah, Siti Hamidatul; Priyambodo, Priyambodo
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11738

Abstract

Research on caffeine biosynthesis in Coffea canephora has expanded across multiple disciplines, yet its global genetic research trends remain insufficiently mapped. This study aimed to analyze the development and thematic structure of genetic research on caffeine biosynthesis in C. canephora using a bibliometric approach. Data were retrieved from the Scopus database through keyword-based sampling and filtered based on relevance to genetic and biosynthetic aspects. Bibliometric analyses were conducted to examine publication trends, authorship patterns, country contributions, and keyword co-occurrence networks. The results show a significant increase in publications since the early 2000s, with dominant contributions from the United States, Brazil, and China. Keyword mapping reveals four major research clusters, including plant metabolism, clinical and nutritional studies, experimental models, and genetic variability. However, studies explicitly focusing on the genetic regulation of caffeine biosynthesis, particularly involving N-methyltransferase genes, remain relatively limited compared to broader multidisciplinary themes.These findings indicate that caffeine research in C. canephora is highly interdisciplinary but still lacks a strong emphasis on genetic mechanisms, highlighting the need for more targeted molecular and genomic studies to support coffee improvement and functional trait development.
Association Between BRCA1 Expression Status and Lymph Node Status in Breast Cancer Patients in NTB Wikananda, Ida Bagus Wisnu; Lestari, Rizka Vidya; Djannah, Fathul
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11744

Abstract

Breast cancer is the most common type of cancer among women in Indonesia, with incidence and mortality rates continuing to rise. One important factor in the development of this disease is the involvement of BRCA1 gene expression. Disruption of BRCA1 gene function plays a role in tumorigenesis. In addition, axillary lymph node status is an important prognostic factor that determines disease stage and progression. However, the relationship between BRCA1 expression and lymph node involvement is still inconsistent, requiring further research to clarify its association. This study aimed to determine the relationship between BRCA1 expression and lymph node status in breast cancer. This research was an analytical observational study with a cross-sectional design. BRCA1 expression was assessed using the H-Score method and classified into high expression (H-Score >100) and low expression (H-Score ≤100). Lymph node status was categorized as negative (N−) or positive (N+). Data from 42 breast cancer samples were analyzed using the Chi-Square test. The results showed that 19 patients (45.2%) had low BRCA1 expression, while 23 patients (54.8%) had high BRCA1 expression. Based on lymph node status, 12 patients (28.6%) were classified as N− and 30 patients (71.4%) as N+. Statistical analysis demonstrated no significant association between BRCA1 expression and lymph node status (p=0.096). In conclusion, BRCA1 expression cannot yet be used as a strong indicator in determining lymph node status in breast cancer patients in NTB.
Multilocus Analysis of DNA Barcodes of matK, rbcL, and ITS in the Asteraceae Family Rosa, Vita; Roslim, Dewi Indriyani; Herman, Herman
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11745

Abstract

Asteraceae is known as compositae which is a cosmopolitan plant group consisting of approximately 1,250 genera and 25,000 species distributed in various regions. Similar morphological characters among species pose challenges in taxonomy and phylogenetics, making the identification process difficult. These limitations of morphological identification can be overcome through a molecular approach using DNA barcoding to reduce errors in species grouping and in determining phylogenetic relationships among plant species in the Asteraceae family through multilocus DNA barcode analysis of matK, rbcL and ITS. This study aims to analyze DNA barcodes matK, rbcL and ITS in the Asteraceae family. DNA sequence exploration were obtained from GenBank through the NCBI website. Analysis of DNA sequence was performed using MEGA11, and phylogenetic tree reconstruction was carried out using the Neighbor Joining (NJ) method in MEGA11 with the p-distance evolutionary model and rapid bootstrap analysis conducted with 1000 replicates. The rbcL sequence had the lowest number of variable sites (13.6%) compared to single sequences and other combinations, while ITS showed the highest number of variable sites (59.3%), and the matK+rbcL+ITS combination produced the most informative phylogenetic grouping because it provided more consistent bootstrap support at the main branches. The multilocus approach increases the resolution in describing phylogenetic relationships among genera within the family Asteraceae.
Analysis of Three DNA Barcoding (matK, rbcL, ITS) in the Anacardiaceae Family Ningrum, Olivi Aruanda; Roslim, Dewi Indriyani; Herman, Herman
Jurnal Biologi Tropis Vol. 26 No. 2 (2026): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i2.11746

Abstract

The Anacardiaceae family is a group of tropical plants that have important economic value, but the identification of its species is often hampered by morphological similarities. Molecular approaches through DNA barcoding are an alternative to improve the accuracy of identification and kinship analysis. The purpose of this study was to analyze the combination of three DNA barcodes, namely (matK, rbcL and ITS) in the Anacardiaceae family. The materials used in this study were DNA sequence databases (rbcL, matK, ITS) from the Anacardiaceae family consisting of eight genera and one outgroup used, namely Pometia pinnata, downloaded from GenBank through the NCBI website. Sequences were aligned using MAFFT v.7, edited using BioEdit v.7.2, and analyzed using MEGA 11 to calculate nucleotide frequencies, nucleotide base pairs, diversity, and phylogenetic tree reconstruction using the Neighbor-joining method with 1000 bootstrapping times. The results showed that the matK sequence, a combination of matK+rbcL sequences, and a combination of matK+rbcL+ITS sequences were the most optimal barcodes for grouping species of the same genus in the Anacardiaceae family and separating out-group species. The ITS sequence, a combination of matK+ITS sequences, and a combination of rbcL+ITS sequences were able to group species of the same genus well but could not separate out-group species. The rbcL sequence was not optimal for use as a single DNA barcode.

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