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Meditory : The Journal of Medical Laboratory
Published by Poltekkes Kemenkes Denpasar
ISSN : 23381159     EISSN : 25491520     DOI : https://doi.org/10.33992/meditory
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
Meditory : The Journal of Medical Laboratory secara khusus berfokus pada masalah utama dalam pengembangan ilmu di bidang laboratorium medis yang mencakup patologi klinik, mikrobiologi, kimia kesehatan dan patologi anatomi.
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Articles 165 Documents
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PREVALENSI INFEKSI SOIL TRANSMITTED HELMINTH PADA PENGRAJIN GENTENG DI DESA WIRUN MOJOLABAN SUKOHARJO MENGGUNAKAN METODE DIRECT DAN INDIRECT Fitria Diniah Janah Sayekti; Muhammad Taufiq Qurrohman; Dwi Haryami
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2428

Abstract

Background : Soil Transmitted Helminths (STH) is an intestinal nematode which in its life cycle requires soil for the egg maturation process. Included in this group are Ascaris lumbricoides, Trichuris trichiura, and Hookworm. Lack of attention to clean living behavior can make a person easily infected with helminthiasis. One profession that is closely related to STH infection is work related to soil, including tile craftsmen.Wirun Village in Mojolaban District, Sukoharjo Regency has residents who partly work as roof tile craftsmen. In carrying out their profession, tile craftsmen sometimes do not use personal protective equipment such as gloves and footwear so that they are in direct contact with the ground and have a potential risk of STH infection. Aims:This study aims to determine the prevalence of Soil Transmitted Helminths (STH) using two methods, direct and indirect.Methode :The direct method is carried out to see the presence of eggs or STH larvae with a faster time. The indirect method is carried out by adding special ingredients that can catch worm eggs or larvae. The indirect technique used in this study was to use saturated NaCl flotation. The results:Based on the research results, it can be seen that 6.67% of the respondents were positively infected with STH using either direct or indirect methods. The STH species found was Ascaris lumbricoides. Based on this, it can be seen that the use of the 2 methods does not affect the different results. Conclusion : The prevalence of soil-transmitted helminth infection among tile craftsmen in Wirun village is low because only 6.67% of respondents were found to be positively infected
IDENTIFIKASI BORAKS DAN FORMALIN PADA MIE BASAH DALAM SOTO MIE Angki Purwanti; Diah Lestari; Salbiah Salbiah
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2454

Abstract

Background: Dozen of soto mie culinary traders in the sub-district of Pondok Melati Bekasi always full of buyers. Preliminary test result using 3 samples of wet noodles which is obtained from culinary seller of soto mie in that area positively identified borax and formalin. Aims: The aim of thisresearch is to identify borax and formalin on wet noodles found in culinary soto mie in the sub-district of Pondok Melati Bekasi. Methods: The research sample was 22 packs of wet noodles obtained from 22 soto mie culinary traders in Pondok Melati sub-district, Bekasi. The borax test used the flame test methode and the curcumin paper test, while the formalin test uses chromotropic acid reagent and Schryvers reagent. Results: Identification of borax on wet noodles in culinary soto mie, by flame test and curcumin paper test resulted in 22 samples (100%) positive for borax. Identification of formalin in the same sample, using chromotropic acid and Schryvers reagent resulted in 22 samples (100%) positive for formalin. Conclusion: Wet noodles in culinary soto mie sold in the region Pondok Melati is all positive for borax and formalin
UJI KUALITATIF ALKOHOL URINE PEMINUM TUAK DALAM MENGETAHUI WAKTU SAMPLING YANG TEPAT I Made Suardika; Lale Budi Kusuma Dewi; Thomas Tandi Manu; I Wayan Getas; Ida Bagus Rai Wiadnya
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2421

Abstract

Background:Accidents and criminal acts can be caused by alcohol use. Alcohol use by a person can be known by analyzing a urine sample. In the analysis of urine samples it is necessary to take the right sampling time. In addition, another factor to consider when detecting alcohol in urine is the time of last consumption. Aims:This study was conducted to determine the appropriate urine sampling time for alcohol consumption. Methode:The method used in this urine alcohol qualitative test uses the modified 2.5% potassium bichromate method in 50% H2SO4. The sample consisted of 10 respondents who drank palm wine after 1 hour, 2 hours and 3 hours of consumption. The results:The qualitative test results of urine drinkers of palm wine drinkers after 1 hour, 2 hours and 3 hours of consuming. 10 respondents obtained the results of 4 positive urine samples 1 hour after consuming and 2 positive urine samples 3 hours after consuming palm wine, as well as sampling in the 2nd hour the test results were negative. Conclusion:The positive test results depended on the habit of consuming, the volume consumed, and the age of the wine drinker
AKTIVITAS ANTI BAKTERI FORMULASI LOTION EKSTRAK ETANOL RIMPANG JERINGAU PUTIH Emilda Sari; Linda Triana; Sugito Sugito; Edy Suwandi
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2292

Abstract

Background: The skin is the most important and largest organ of the human body. The skin needs so much attention, both in terms of disease and health,because it turns out that the skin itself is quite susceptible to bacterial and fungal attacks. Therefore, the formulation of lotions for the skin, namely ingredients that can kill bacteria or fungi and are safe for the skin can be justified. Natural ingredients that have properties as antibacterial ingredients for the skin are White Jeringau. Aims:The purpose of this study was to explain the differences in the inhibition of the lotion formulation of the ethanol extract of the White Jeringau Rhizome against the growth of Staphylococcus aureus bacteria using the diffusion method. Methods:The research method used is a quasi-experimental with purposive sampling technique. The sample in this study was the lotion of the ethanol extract of the Jeringau Putih rhizome with concentrations of 40%, 50% and 60% which were replicated 9 times each so that the number of samples in this study were 27 samples. Furthermore, the sample was tested for inhibition of Staphylococcus aureus bacteria by diffusion method. Results:Based on the results of this study in 2022, antibacterial analysis of the white jeringau extract lotion sample formula I, formula II and formula III against Staphylococcus aureus bacteria using the Kirby-bauer diffusion method showed the results of the inhibition zone diameter, namely in formula I the average inhibition zone formed was 11 .50 mm, in formula II it is 13.61 mm and in formula III it is 14.50 mm. According to Davis and Stout (1971) the inhibitory response by active ingredients is grouped into 4categories, namely weak activity (? 5 mm), moderate (5-10 mm), strong (10-20 mm), and very strong (? 20 mm). Conclusion:Based on this classification, white jeringau extract lotion formula I, formula II and formula III are categorized as strong. Formula Ihad the best inhibitory response because the concentration of the extract was the smallest and had a strong category of inhibition compared to formula II and formula III which had a higher concentration and had the same inhibitory power. the results of statistical test analysis using the Kruskal Walis test obtained p value = 0.000 0.05 and it was concluded that there were differences in the white jeringau rhizome lotion formula I, II, and III in inhibiting the growth of Staphylococcus aureus bacteria.
PENGARUH WAKTU INKUBASI TERHADAP DIAMETER ZONA HAMBAT ANTIBIOTIK PADA UJI SENSITIVITAS BAKTERI KLEBSIELLA PNEUMONIA Atik Martsiningsih; Suyana Suyana; Aisyiah Noviani; Ullya Rahmawati; Sujono Sujono; Fardhiasih Dwi Astuti
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2361

Abstract

Background: Several analytical factors can affect the diameter of the inhibition zone; This factor must beinvestigated toguarantee thevalidityofthe sensitivitytestresults. Aims: This study was to determine the pattern of influence of variations in incubation time on thediameterofthe antibiotic inhibition zone in the sensitivity test of Klebsiella pneumoniae. Methode: this research is a pre experimental design with a Static Group Comparison design.The diameter of the antibiotic inhibition zone with variations in incubation time was measured and analyzeddescriptively.The results: The diameter of the inhibition zone on the variation of the incubation time showed a patternof the influence of the incubation time on the diameter of the antibiotic inhibition zone in the bacterialsensitivity test, namely at 6-18 hours, an increase in the diameter of the inhibition zone and 18-48 hours a decrease in the diameter of the zone. Inhibite ach antibiotic. Conclucion:This study concludes that the reisasignificant effect with the pattern of theinitial incubation time(6-18hours) affecting the broader or larger diameter of the inhibition zone and the subsequent incubation time of 18-48 hours decreasing the diameter of the inhibition.Keywords: Inhibition zone diameter, incubation time, sensitivity test
AKTIVITAS ANTIOKSIDAN DAN KADAR TOTAL FENOL MAKROALGA PERAIRAN TANJUNG BENOA, BALI Jannah Sofi Yanty; I Wayan Karta
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2368

Abstract

Purpose : The purpose of this study was to determine antioxidant activity of macroalgae in Tanjung Benoa, Bali, Method : In this research, the antioxidant activity of macroalgae in Tanjung Benoa, Bali, will be tested using the DPPH method. Total phenolic test / TPC (Total Phenolic Content) test was also carried out on macroalgae samples using the Folin-Ciocalteau method. TPC value is expressed as the equivalent of mg gallic acid (GAE)/g dry weight of the sample. Result: Samples obtained at the time of sampling were 10 macroalgae samples consisting of 5 red macroalgae (rhodophyta), 4 green macroalgae (chlorophyta) and 1 brown macroalgae (phaeophyta). The results of the antioxidant analysis showed that the 4 macroalgae that had the greatest percentage of inhibition were the macroalgae Chondrus crispus, Sargassum sp., Gracilaria sp and Acrosorium sp.1. The results of total phenol analysis showed that the highest levels of total phenol were found in macroalgae Sargassum sp., Gracilaria sp. and Acrosorium sp. 1. Conclucion : Total phenolic content of most of the macroalgae samples had a positive correlation with antioxidant potential.Keywords: macroalgae, antioxidants, total phenols, DPPH, Tanjung Benoa
UJI EFEKTIVITAS EKSTRAK RIMPANG LENGKUAS MERAH (Alpinia Purpurata K. Schum) TERHADAP Candida albicans Siti Juariah; Tri Setia Ningrum; Eli Yusrita
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2303

Abstract

Background: Infectious diseases are diseases caused by the entry and breeding of microorganisms, namely bacteria, viruses, fungi, prions and protozoa into the body, causing organ damage. Candidiasis is a disease caused by the fungus Candida albicans, commonly found in the skin, mucous membranes and digestive tract. Infection control usually uses antibiotics, but continuous use can lead to resistance to microbes, the use of alternative materials is needed. Red galangal is one type of spice that has been widely used as a phytopharmaceutical product or a product that utilizes vegetable resources as a source of medicinal ingredients. Aim:The purpose of the research was to determine the effect of red galangalrhizome extract on the growth of Candida albicans. Methods:The methods used are experimental and disc diffusion method. Based on the results of the study, it can be seen that there is an inhibitory response seen from the difference in the distance of theinhibition zone between the negative control and several other concentrations. Resulsts:The results of the measurement of the inhibition of Candida albicans using concentrations of 10%, 20%, 40%, and 60% respectively 11.3 mm, 13 mm, 13.3 mm, 14.3 mm. positive control (ketoconazole) formed an inhibition zone diameter of 8.6 mm while negative control did not form an inhibition zone. Conclusion:Based on the results of the study, it can be concluded that the red galangal rhizome extract (Alpinia purpurata K.Schum) has the ability as an antifungal against the growth of Candida albicans.
PERANAN SISTEM IMUNITAS MELAWAN INFEKSI TUBERKULOSIS PARU-PARU Widaninggar Rahma Putri
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2373

Abstract

Tuberculosis, an infectious disease caused by Mycobacterium tuberculosis evoked more than 1 million mortality every year and infected more than a quarter population worldwide. Host immunity systems against tuberculosis provided by innate immunity and adaptive immunity. Molecules and cells in both of immunity systems undertaking this mechanism and initiate each other facing infection from very first bacterial exposure inside the host. Immunity systems against bacteria development from inhalation step through granuloma initiation and antibody anti-tuberculosis. Host’s mechanism against tuberculosis is urge to presumed in order to develop strategies based on immunology to decreasing tuberculosis incidence worldwide
OPTIMASI FORMULASI SABUN CAIR ANTIBAKTERI VARIASI KOMBINASI EKSTRAK DAUN LEGUNDI DAN SIRIH Ida Bagus Oka Suyasa; Putu Ayu Suryaningsih; Trisna Bagus Wibawa; Putu Diah Wahyuni; Ni Made Deliabudi Apriliapatni; I Made Krisna Paramartha; Pande Putu Wahyu Pradiptha Nugraha
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2456

Abstract

Background: Legundi leaves and betel leaves are widely known as plants that contain medicinal properties. The extract of these two leaves is useful as an antibacterial agent. Aims: This research was conducted with the aim of knowing the antibacterial activity of liquid soap containing the active ingredients of the two leaves. Method:The variables studied included: organoleptic properties, pH value, foam height, free alkali content, specific gravity and antibacterial activity. The type of research used istrue experimental research. The research design is Postes only control design. Data collection was carried out by means of measurement, while data analysis was in the form of descriptive and analytic. Conclucion:The conclusions obtained include liquid soap preparations in various combinations of legundi leaf and betel leaf extracts that meet SNI requirements, the bacterial inhibition zone is classified as strong but the most optimal is F3, there are differences in the diameter of the inhibition zone for Staphylococcus aureus bacteria growth in liquid soap with various combinations of extracts. The recommendations given for the research to continue up to the irritation test, it is necessary to add fragrances to make it more fragrant.
KADAR GLUKOSA PADA SERUM LIPEMIK DENGAN PENGGUNAAN POLYETHYLENE GLYCOL 6000 8% DAN HIGH SPEED SENTRIFUGASI Martha Atik Martsiningsih; Sujono Sujono; Bambang Supriyanta; Menik Kasiyati; Catherine Surya; Budi Martono; Budi Setiawan
Meditory : The Journal of Medical Laboratory Vol 11, No 1 (2023): Meditory, Volume 11 No. 1 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i1.2425

Abstract

Bacground: Lipemic serum is a problem in many laboratories because it can cause the results of glucose levels in the serum to be falsely high. The examination uses a photometric method, which can cause interference with wave lengths and light scattering. As an alternative to high-speed centrifugation to remove lipemic serum, another method for handling lipemic serum is precipitation, which is carried out by processing polyethylene glycol 6000 8%.Aims: This research wants to know the differences in the handling of lipemic serum before and after being processed using polyethlyene glycol 6000 8% and high-speed centrifugation.Method: This type of research is an experiment with Static Group Comparison. Research conducted at the Clinical Pathology Laboratory Installation at the Gadjah Mada University Academic Hospital in November 2022. The sample of this study was lipemic serum with a total of twenty samples obtained from the rest of the examination in two Clinical Laboratory Installations Hospitals in Yogyakarta. Research data were analyzed using the Paired T-Test and Independent T-Test. Result: The statistical analysis (p = 0.000) 0.05 revealed that there is a difference in lipemic serum glucose levels before and after PEG 6000 8% processing and high-speed centrifugation. Before processing with polyethylene glycol 6000 8% and high-speed centrifugation, the average yield of glucose levels in lipemic serum was 116.3 mg/dl. The average glucose level before and after PEG 6000 8% processing was 108.65 mg/dl. Before and after high-speed centrifugation, the average glucose level was 114.1 mg/dl.Conclusion: Overall, both the PEG 6000 8% processing and the high-speed centrifugation resulted in a significant decrease in lipemic serum glucose levels. These methods appear to be effective in reducing the interference caused by lipemia and improving the accuracy of glucose measurements in the serum.

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