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Meditory : The Journal of Medical Laboratory
Published by Poltekkes Kemenkes Denpasar
ISSN : 23381159     EISSN : 25491520     DOI : https://doi.org/10.33992/meditory
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
Meditory : The Journal of Medical Laboratory secara khusus berfokus pada masalah utama dalam pengembangan ilmu di bidang laboratorium medis yang mencakup patologi klinik, mikrobiologi, kimia kesehatan dan patologi anatomi.
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Articles 165 Documents
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IRRITATION TEST OF LIQUID ANTIBACTERIAL SOAP COMBINING LEGUNDI AND GREEN BETEL LEAVE EXTRACTS ON WHITE RATS (Rattus norvegicus) Suyasa, Ida Bagus Oka; Dewi, Ni Nyoman Astika; Apriliapatni, Made Delia Budi; Bryan, Surya Natallia
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4067

Abstract

Background: Soap is a cleanser that is made with a chemical reaction. Soap preparations with active ingredients derived from nature are also widely circulated. Natural ingredients that have many active substances are legundi leaves and green betel leaves. Two antimicrobial agents from these ingredients working simultaneously on a homogeneous microbial population will have a synergistic effect.Objective: This study aims to determine the safety of using an antibacterial liquid soap formula with a combination of legundi and betel leaf extracts.Methods: This study is an experimental research that includes physicochemical testing of the soap formula, organoleptic evaluation, and irritation testing on white rats (Rattus norvegicus) using the Draize method with a posttest-only control group design. This research was analyzed descriptively. This study adheres to the ethical principle of beneficence, prioritizing the well-being and safety of all participants.Results:  Organoleptic test, pH test, homogeneity test, and water content test meet Indonesian National Standard requirements, and there is no irritation in experimental animals.Conclusions: An antibacterial liquid soap formula with a combination of legundi and betel leaf extracts is safe.
FOOD SAFETY STUDY IN TERMS OF MICROBIOLOGY IN PORK SATAY IN SANGEH TOURISM VILLAGE Ditiarini, Ni Putu Vriska Putri; Sudita Puryana, I Gusti Putu; Tamam, Badrut
Meditory : The Journal of Medical Laboratory Vol 11, No 2 (2023): Meditory Volume 11 No. 2 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i2.3066

Abstract

The application of tourism health, such as food safety, is an important matter to pay attention to. Based on the annual report (BPOM, 2019), there are 373 cases of food poisoning in Bali, so Bali ranks fourth with the highest cases of food poisoning in Indonesia. The type of research used is observational. This research was conducted by testing samples to determine microbial contamination on pork satay as well as observing and recording food safety scores where pork satay is sold. The design used was cross-sectional because all research subjects were observed at one time. Food safety scores were tested, and TPC, MPN, and E. coli were tested so that 9 samples (56.25%) were categorized as vulnerable to safe consumption. 16 samples of pork satay met the minimum requirements for microbial contamination, ranging from 20/g to 60,000/g. 1 sample of pork satay (6.25%) with the amount of coliform contamination exceeding the standard, namely 438/gr. There were 6 samples of pork satay (37.5%) positive for E. coli. Based on the results of the Kruskal-Wallis test for total microbial contamination based on the food safety score, the results showed that there was a difference in the results of the microbial contamination test and E. coli with a food safety score. Coliform bacteria contamination based on the food safety score showed that there was no difference from the results of the MPN test with the food safety score based on the Asymp.sig value 0.05. Even so, there are still many traders who have not implemented personal hygiene, so it is necessary to carry out food safety training, monitoring, and developing food quality on a regular basis.
ANTIBACTERIAL ACTIVITY SCREENING OF BACTERIAL ISOLATES ASSOCIATED WITH SEAWEEDS Caulerpa lentillifera AR, Sunthio Barran Cia; Indraningrat, Anak Agung Gede; Widhidewi, Ni Wayan; Wijaya, Made Dharmesti; Antara, Kadek Lila; Choe, Daegeun
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4051

Abstract

Background: Caulerpa lentillifera is a species of green algae that has been cultivated widely in Indonesia for its nutritional contents and health benefit. This seaweed builds a strong association with bacterial community which play an important role in synthesizing various secondary metabolites including antibacterial activity. Aims:The research aim to explore the symbiotic relationship between C. lentillifera and its associated bacterial community, focusing on the potential of these bacteria to produce antibacterial metabolites.Methods:Bacterial cultivation was performed on three different media namely nutrient agar, plate count agar, and zobell marine agar. The obtained bacterial isolates were identified morphologically and subsequently were screened for their antibacterial activity based on perpendicular cross streak method against Staphylococcus aureus ATCC 25923, Streptococcus mutans FNCC 0405, Escherichia coli ATCC 25922, and Klebsiella pneumoniae ATCC 700603. Results: 20 out of 28 bacterial isolates inhibited against at least one of the bacterial targets. Bacterial isolate encoded as NAR9 showed as the most promising bacterial isolates with length inhibition of 19 mm against S. mutans FNCC 0405. Amplification of 16S rRNA gene fragment of the NAR9 bacterial isolate followed by Sanger sequencing showed that the isolate NAR9 has been phylogenetically related to Marinobacter hydrocarbonoclasticus. Conclusion: Overall, this research provides a preliminary result of the untapped potential of bacteria associated with C. lentillifera as the source of antibacterial activity. 
THE EFFECT OF SAMPLE TEMPERATURE VARIATIONS ON MEASURED URINE ALCOHOL CONCENTRATION: A SYSTEMATIC REVIEW Kurniawan, Salsabila Yunita
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3756

Abstract

Background: If an alcohol-containing urine sample cannot be analyzed immediately or requires shipping, it should be stored at an appropriate temperature. Pre-analytic is a stage that needs serious attention where at this stage the risk of errors often occurs. Error in storage samples can affect the results of alcohol concentration, so a laboratory technician needs to understand urine sample storage to measure alcohol concentration. Aims: Knowing the best storage of urine temperature in measuring alcohol concentration. Methods: This study used a systematic literature review of urine samples to measure alcohol concentration. The results: The storage temperature on measured urine alcohol concentration was carried out from 1977 – 2023. It can be seen that in some studies regarding storage temperatures in urine using at least 5 temperatures: -20oC, 4oC, 6oC, 14oC, ±20oC. The temperature storage at 4oC can prevent the production of ethanol due to microorganism activity, it will maintain the stability of alcohol concentration in urine. Conclusions: A temperature of 4oC is highly recommended for measuring alcohol concentration in urine samples.
THE RELATIONSHIP OF BREAKFAST HABITS WITH HEMOGLOBIN LEVELS IN CLASS XI STUDENTS AT SMA NEGERI 7 DENPASAR Satwikha Dewi, I Gusti Agung Ayu; Dwijastuti, Ni Made Sri; Septiasari, Ni Putu Senshi; Widiantari, Ni Putu; Eka Putri, Ni Putu Puniari
Meditory : The Journal of Medical Laboratory Vol 12, No 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i1.3215

Abstract

Introduction: Hemoglobin levels are a clinical parameter in routine hematology examinations which are used to determine whether there are health problems. Adolescents need to pay attention to hemoglobin levels because hemoglobin levels are crucial for supporting the physical, mental and academic well-being of adolescents during this important phase of their growth and development. Method: This research method is observational research with a cross sectional approach. This research was conducted at SMA Negeri 7 Denpasar, Wednesday 24 May 2023. The sampling technique used was purposive sampling with a sample size of 42 students in class XI of SMA Negeri 7 Denpasar. Results: There were 8 students in class Students who did not have breakfast had normal and high hemoglobin levels, while students who had breakfast had low, normal and high hemoglobin levels. The results of the analysis using the Somers'D test showed that there was no relationship between breakfast habits and hemoglobin levels with p value = 0.188 or p 0.05. Conclusion: Breakfast habits do not directly affect hemoglobin levels.Keywords : breakfast, hemoglobin, teenager
THE EFFECT OF SUBTOTAL NEPHRECTOMY PROCEDURE ON THE GLOMERULOSCLEROSIS INDEX Peranawa, I Wayan Yoga Diva; Witari, Ni Putu Diah; Cahyawati, Putu Nita
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4039

Abstract

Background:  Chronic kidney disease is one of the kidney disorders with a steadily increasing incidence rate.Objective: This study aims to determine the effect of subtotal nephrectomy on the renal glomerulosclerosis in Mus musculus L. at 7, 14, and 28 days post-operation. Methods: This true-experimental study with a randomized post-test-only control group design used 16 white mice divided into four treatment groups (SO, SN7, SN14, and SN28). Kidney specimens were stained using Hematoxylin-Eosin and imaged to assess the glomerulosclerosis index. The results were analyzed using one-way ANOVA.Results:  The findings showed that glomerulosclerosis could be observed as early as 7 days post-procedure. The highest score was found in the SN28 group (3.55 ± 0.129). A significant difference was observed between the SO and SN7, SN14, and SN28 groups (p 0.05). Significant differences were also found between the SN7 and SN14 and SN28 groups (p 0.05), while no significant difference was observed between the SN14 and SN28 groups.Conclusions: These results indicate that subtotal nephrectomy can induce glomerulosclerosis as early as day seven post-operation. The highest glomerulosclerosis index was observed on day 28, although statistically, it did not differ significantly from the index on day 14.
PHYTOCHEMICAL COMPOUND LEVELS IN BAMA LEAF EXTRACT AND ITS INHIBITORY ACTIVITY AGAINST Salmonella Thypimurium ATCC 19430 Suryaningsih, Putu Ayu; Oka Suyasa, Ida Bagus; Fridayanti, Komang Dewi
Meditory : The Journal of Medical Laboratory Vol 11, No 2 (2023): Meditory Volume 11 No. 2 Tahun 2023
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v11i2.2925

Abstract

Diarrhea is one of the intestinal infection symptoms that can cause poor absorption to loss of nutrition if not treated immediately. Salmonella spp. is 1 of the 4 fundamental global causes of diarrhoeal diseases and antibiotics have been widely used to treat infections caused by this group of bacteria. WHO reported that the antibiotic resistance of Salmonella spp. has increased in past years. For those reasons, efforts have been made to discover new antimicrobial compounds from various sources, including the ethanol extracts of Bama leaves. Therefore, this study aimed to determine the phytochemical compounds (flavonoids, tannins, and phenols) in the ethanol extract of Bama leaves and their inhibitory effect against the bacteria Salmonella typhimurium which causes diarrhea. Design method used is RAL 5 x 4, 5 treatments were concentration of Bama leaf extract 6,25%, 12,5%, 25%, 50% and 75% and 4 replicates for each test. The phytochemical quantitative analysis showed bama leaf extract had 520,99 mgQE flavonoid / 100 gram, 1465,19 mgTAE tannin/100 g and 16531,78 phenol/100 g extract. All contrentration of bama leaf extract could not inhibit the growth of Salmonella thypimurium ATCC 19430. 
THE EFFECT OF TEMPERATURE AND STORAGE DURATION OF WASHED RED CELLS ON THE NUMBER OF ERYTHOCYTES Andina, Chika Alivia; Noviar, Ganjar; Marliana, Nina; Hayati, Eem
Meditory : The Journal of Medical Laboratory Vol 12, No 2 (2024): Meditory, Volume 12 No. 2 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i2.3428

Abstract

Background: Blood services in Indonesia, including processing and storing donor blood, aim to ensure blood quality remains optimal. Washed Red Cell blood components are used for patients with severe allergies due to repeated transfusions, have a limited shelf life and are susceptible to secondary infections. Aims: Blood services in Indonesia, including processing and storing donor blood, aim to ensure blood quality remains optimal. Washed Red Cell blood components are used for patients with severe allergies due to repeated transfusions, have a limited shelf life and are susceptible to secondary infections. Methods: This research design uses a quasi-experiment with serial time measurements. Samples were taken from one bag of WRC donor blood from the Bandung City PMI Blood Transfusion Unit. Specimens were stored at room temperature (20-25°C) and refrigerator temperature (4-6°C) for 0, 3, 6, and 9 hours, and the number of erythrocytes was measured using a Haematology Analyzer. The results of the study showed that the average number of erythrocytes stored at room temperature was 7.54×10⁶/µL at the 0 hour examination, 6.37×10⁶/µL at the 3 hour examination, 5.40×10⁶/µL at the 6 hour examination. and at the 9 hour examination 4.56×10⁶/µL. Meanwhile, the average number of erythrocytes stored at refrigerator temperature was at the 0 hour examination 7.65×10⁶/µL, at the 3 hour examination 7.43×10⁶/µL, at the 6 hour examination 7.25×10⁶/µL, and at 9 hour examination 7.02×10⁶/µL. The results of the General Linear Model (GLM) statistical test show a sig value = 0.000 0.05. Conclusions: temperature and storage time have a significant influence on the number of erythrocytes.
CORRELATION BETWEEN ERITHROCYTES SEDIMENTATION RATE AND HIGH SENSITIVITY C-REACTIVE PROTEIN LEVELS IN PATIENTS WITH CORONARY HEART DISEASE Sanaky, Marisca Jenice; Grace Orno, Theosobia
Meditory : The Journal of Medical Laboratory Vol 12, No 1 (2024): Meditory, Volume 12 No. 1 Tahun 2024
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v12i1.2469

Abstract

Coronary Heart Disease (CHD) is one of the diseases that currently threatens public health. One of the biggest triggers for CHD is atherosclerosis or narrowing of the arteries which begins with an inflammatory process. Erythrocyte Sedimentation Rate (ESR) and High Sensitivity C-reactive Protein (Hs-CRP) are acute phase proteins that will appear when an inflammatory process occurs. This study aims to assess the correlation between ESR and Hs-CRP as a marker of inflammation in CHD patients. The research method used was laboratory observation with a cross sectional design. The results of the normality test using shapiro-wilk show a value of p0.05, which means that the data is normally distributed. The average ESR value was 14.25 mm/hour in the male gender category and 26.45 mm/hour in the female gender category. The results of the Spearman-rho correlation test showed that there was a moderate correlation between LED and Hs-CRP (r=0.564). The conclusion of this study is that there is a correlation between ESR and Hs-CRP values in CHD patients. The conclusion of this study is that there is a correlation between ESR and Hs-CRP values in CHD patients.
PHYTOCHEMICAL PROFILING AND ANTIOXIDANT PROPERTIES OF ETHANOL EXTRACT OF TAKOKAK EGGPLANT LEAVES (Solanum torvum) Mita Efianti, Ni Wayan; Ratih, Gusti Ayu Made; Astika Dewi, Ni Nyoman
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.3980

Abstract

Background: Takokak eggplant (Solanum torvum) has been traditionally used in medicine, and its secondary metabolites possess antioxidant potential.Objective: This study aimed to determine the phytochemical profiling and analyze the antioxidant properties of Takokak eggplant leaf extract (Solanum torvum) using ethanol solvent.Methods: An experimental research design was used. Antioxidant activity was tested using the DPPH method.Results:  Phytochemical screening test of the 70% ethanol extract revealed the presence of flavonoids, terpenoids, saponins, and tannins, while the 96% ethanol extract of Takokak eggplant leaves (Solanum torvum) contained only flavonoids, terpenoids, and tannins. Antioxidant activity tests showed that the IC50 (Inhibition Concentration) value of 70% ethanol extract was 49.9 ppm (very active), and 96% ethanol extract was 65.7 ppm (active). The AAI (Antioxidant Activity Index) value of the 70% ethanol extract was 0.80, while the 96% ethanol extract was 0.60.Conclusions: Takokak eggplant leaf extract (Solanum torvum) with 70% ethanol had higher phytochemical content and antioxidant activity than 96% ethanol.

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