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Biota
ISSN : 20863586     EISSN : 24608483     DOI : -
Focus and Scope of Biota on Bioscience, includes: cell biology and genetics, biodiversity, ecology, physiology and biotechnology. Biota covers all life forms, including microbes, fungi, plants, virus, animal and human. Focus and Scope of Biota on Biology Education, includes: Experimental Research, Class Action Research, Qualitative Research, Quantitative Research, Model Learning Development, Media Learning Development, and Learning Evaluation.
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Articles 137 Documents
Curcumin Effectivity on Hepar and Reproductive Organ Recovery Male Mice (Mus musculus L) after Methoxychlor Exposure Mahriani Mahriani; Susantin Fajariyah; Eva Tyas Utami
Biota Vol 13 No 1 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i1.196

Abstract

Methoxychlor (MXC) is an insecticide (DDT derivates) that has the potential for bioaccumulation in mammal and causes a disruptive effect on the hepar and reproductive system. This study was done to find out the benefits of curcumin as a natural ingredient to overcome the negative impact of Methoxychlor (MXC) on hepar and male reproductive organ of Balb’C mice (Mus musculus L). The study was carried out in a Completely Randomized Design (CRD) Posttest Only Control Group Design used four treatments and six replications. The curcumin treatment after administration of MXC was carried out by gavage with curcumin doses: 0.05; 0,1; and 0.2 mg/g body weight, every day for two weeks, respectively. Histological observations of the liver, and testis was performed using the paraffin method and Hematoxylin Eosin stained. The results showed that MXC exposure caused liver disruption by increasing the number of pycnotic necrotic hepatocytes and hydrophic degeneration hepatocytes. On the male reproductive organ, MXC caused testis impairment by reducing the number of Sertoli cells and Leydig cells, spermatogenic cell counts, and the diameter of seminiferous tubules. The administration of curcumin at doses of 0.1 mg/g bw in mice exposed to methoxychlor can reduce the number of hydrophic degeneration hepatocytes and tend to reduce the number of pycnotic hepatocytes; and also increase the number of Sertoli cells, the number of spermatogenic cells, and the diameter of the seminiferous tubules, and tend to reduce the amount of Leydig cells. Curcumin treatment tends to recover hepar dan testis disruption of mice that were exposed by MXC.
In-silico Approach for The Prediction of Chlorogenic Acid as PPAR-γ Activator Yohanes Bare; Mansur S; Sukarman Hadi Jaya Putra; Margaretha Rika W G L; Dewi Ratih Tirto Sari
Biota Vol 13 No 1 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i1.197

Abstract

Coffee is one of the essential crops commonly cultivated in Indonesia. Coffee contains diverse bioactive compounds, which are associated with human health benefits. One of the compounds is Chlorogenic acid, which able to decrease the risk of type 2 diabetes mellitus (T2DM). However, the mechanism of chlorogenic acid toward anti-diabetes still unclear. This study aimed to analyze and investigate the potential role of chlorogenic acid as anti-diabetes through their interaction with Peroxisome proliferator-activated receptor gamma (PPAR-γ) as an enzyme to phosphorylate and regulate the mechanism of T2DM. The physicochemical properties of chlorogenic acid also performed in this study. The PPAR-γ was downloaded from the PDB database, and the chlorogenic acid was retrieved from the PubChem database. The protein and ligand were prepared using the PyRx program and were docked using Hex 8.0.0 software. Discovery Studio client 4.1 software was used to analyze the interaction between chlorogenic acid and PPAR-γ protein. Based on the physicochemical properties, chlorogenic acid is highly permeable to the cell and easily absorbed. Thirteen amino acid residues of PPAR-γ (GLN410, SER394, ASP396, GLY395, GLU407, LEUA401, LEU400, VAL403, LYS373, LYS438, LEU377, LYS434, and GLY437) were identified on the chlorogenic acid-PPAR-γ interaction. Interestingly, the kind of interactions, including hydrophobic interaction, hydrogen bond, and van der Waals, which are supported by the tight interaction. Our study indicated that chlorogenic acid might have anti-diabetes activity through PPAR-γ interaction.
Biolarvacide of Culex quinquefasciatus Rian Oktiansyah; Anggun Wicaksono; Fahmy Armanda; Amin Nurokhman; Ummi Hiras Habisukan; Kurratul ‘Aini; Yustina Hapida
Biota Vol 13 No 1 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i1.234

Abstract

Biolarvicide is the larvicide derived from plants and relatively safe. The objective of this study was to determine the effect of pletekan leaf powder (Ruellia tuberosa), mimosa (Mimosa pudica), papaya (Carica papaya), and guava (Psidium guajava) on Culex quiquefasciatus larval mortality. The method was an experimental with a completely randomized design consisted of 5 treatments (0 gr, 0,25 gr, 0,5 gr, 0,75 gr, and 1 gr) and 5 repetitions for each plant. The data were analyzed by analysis of variance. Based on the results, leaf powder used in the study, namely guava leaves powder (1 gr), mimosa (1 gr), leaves of pletekan (1 gr), and leaves of papaya (1 gr) showed a very significant effect (P < 0,05) on Culex quiquefasciatus larval mortality. It can be concluded that the pletekan, mimosa, papaya, and guava had the potential as biolarvicide of Culex quinquefasciatus.
Cytotoxicity Assay of 2,4-Dihydroxide-4’-Methoxychalcone Against Cervical (HeLa) Cancer Cell by MTT Assay Novia Suryani; Sabirin Matsjeh; Mutmainah Mutmainah; Daratu Eviana Kusuma Putri Eviana Kusuma Putri; Damayanti Iskandar; Syarifah Asyura
Biota Vol 13 No 1 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i1.235

Abstract

Chalcone is one of the phenolic group secondary metabolic with numerous biological activity. Many studies have shown that chalcone derivatives compound has anticancer, anti-inflammatory, antimalarial, and antibacterial activities. The purpose of this research was to study the prediction potency unsaturated carbonyl system of chalcone derivative against the HeLa cell by MTT assay. Those activities assumed can inhibit the mechanism action of NF-kB that caused cervical cancer. The 2,4-dihydroxide-4’-methoxychalcone has done synthesis as a target compound by a sonochemical for 7 hours. The results showed that chalcone derivative most active against the HeLa cell.
Foot Print of Macro Fungi in The Coastal Forest of Bama, Baluran National Park, East Java Sri Rahayu; Annisa Wulan Agus Utami; Cahyo Nugroho; Endah Yuliawati Permata Sari; Kusuma Wardani Lydia Puspita Sari; Maghfirah Idzati Aulia; Noor Adryan Ilsan
Biota Vol 13 No 1 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i1.242

Abstract

Baluran National Park, West Java, as one of the conservation sites in Indonesia, has the attraction of the varied types of ecosystems, including fungi. This study aimed to analyze the diversity of fungi in Bama Coastal Forest, Baluran National Park. The method was explorative with plot purposive sampling technique. Parameters in this study include abundance, dominance, and diversity of fungi enriched with physical parameters of humidity and temperature. The fungi were documented and macroscopically observed. Data were analyzed using the abundance index, dominance index, and diversity index. This research identified 18 types of macrofungi in Bama Coastal forest, Baluran National Park East Java including Ganoderma, sp, Hexagonia tenuis, Trametes hirsute, Phellinus sp.1 and sp.2, Ganoderma applanatum, Phellinus igniarius, Pycnoporus cinnabarinus, Daedalea quercina, Tyromyces chioneus, Microporus xanthopus, Calvatia sp., Irpex lacteus, Trichaptum sp., Lentinus sp. Poria corticola, Tyromyces sp., and Lichemomphalia sp. One fungi species (Ganoderma sp.) has the highest abundance index (27.62). The Medium abundance index was found in three species (Hexagonia tenuis, Tyromyces chioneus, Tyromyces sp.) while the rest fungi were in low abundance index. Dominance index of all fungi species identified in Bama was in low category (D<0.5) with medium diversity (2.30). It can be concluded that the diversity of microfungi in the coastal forest of Bama, Baluran National park was a distribution medium of individual of each species and medium community stability. Environmental conditions in the Bama Coast Forest with 93% humidity and an average air temperature of 26-27oC could support for the fungi to grow quite well.
Identification of Edible Macrofungi at Kerandangan Protected Forest & Natural Park, West Lombok Regency, Indonesia Ahmad Hapiz; Sukiman Sukiman; Faturrahman Faturrahman; Bagus Priambodo; Facthur Rohman; Hendra Susanto; Wira Eka Putra
Biota Vol 13 No 1 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i1.250

Abstract

Indonesia is considered as a mega-biodiversity country that has a massive amount of vascular and non-vascular plants. The tropical environment condition of Indonesia could support the growth of macrofungi. Information about edible macrofungi from the Forest of Lombok Island is based on limited data. This research aims to characterize the edible macrofungi at Kerandangan Protected Forest & Natural Park, West Lombok Regency, Indonesia. This research was a descriptive and explorative study. The edible mushrooms were observed through the Cruise method by following the particular track inside the forest. The sample found in the forest then documented and evaluated. A morphological analysis procedure was performed to assess the profile and similarity between the microscopic evaluations with the mushrooms identification book. In this study, we also offered a phylogeny analysis based on morphological characters similarity. The Dendogram tree was reconstructed using PAST 3.0. software. The result showed that there are eight species of edible mushrooms found that were group into Basidiomycota, namely, Termitomyces clypeatus, Termitomyces umkowaan, Termitomyces sp.1, Pleorotus flabelatus, Pleurotus ostreatus, Coprinus desimenatus, Tremella fuciformis, and Polyporus sp. All of them involved in Basidiomycota. Based on characterization and identification, the species are grouped into three Ordos and five Families. Most of the edible macrofungi found at Kerandangan Protected Forest & Natural Park growth at soil and deadwood.
The Ability of Soil Candida albicans Secreted Potential Protease and Lipase Ririn Puspadewi; Putranti Adirestuti; Mira Andam Dewi; Wakilatul Hasanah
Biota Vol 13 No 2 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i2.245

Abstract

Candida spp secreted a different kind of extracellular enzymes. Protease and lipase are the enzymes that are commonly used in the food and pharmaceutical industries. This study aimed to examine protease and lipase activity of Candida tropicalis isolated from the soil of the Medicinal Plant Garden of the Faculty of Pharmacy, Universitas Jenderal Achmad Yani, Indonesia. Candida isolate was subjected to the fermentation process to obtain bioactive metabolites. The result was analyzed using ANOVA within a 5% interval of confidence, continuing with PostHoc. The result showed the Candida tropicalis metabolite giving the best proteolytic index value (0,6556 ± 0,0090) U/mL. The metabolite isolate of Candida tropicalis had the highest activity, amounting to 5,776 ± 0,495 U/mL. The best results of lipolytic index value (0,394 ± 0,053) U/mL. The Candida tropicalis metabolites produced the highest lipase enzyme after nine days of fermentation in 5.2917±0,0167 U/mL.
Rheotaxis Response Based on Sexual Dimorphism in the Green Swordtail Fish, Xiphophorus hellerii Muhammad Irsyad Abiyusfi Ghafari; Vita Fitrianti
Biota Vol 13 No 2 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i2.298

Abstract

Morphological distinctions between males and females of a species are referred to by sexual dimorphism. It may result from various selection pressures affecting either sex or both and may occur in any dioecious species, including Green Swordtail fish, which are sexually reproductive. This study examined the different rheotaxis responses of Xiphophorus hellerii based on different sexes and morphological features. We analyzed ten adult males, ten gravid females, and ten non-gravid females of Xiphophorus helleri collected down the river and transferred into the column. We counted the number of the individual that performed positive rheotaxis (+), negative rheotaxis (-), and indifference response (0). The result showed different rheotaxis responses shown by male, non-gravid female, and gravid female X. hellerii. The highest percentage of positive rheotaxis response (movement against the current) was shown by non-gravid female X. hellerii, reaching up to 89%. Morphological differences between male, non-gravid female, and gravid female X. hellerii appear to affect the orientation and ability of X. hellerii in giving response against current and certainly has an impact on their survival in nature.
Diversity, Abundance, and Distribution Patterns of Natural Enemy Insects on Chili (Capsicum annum L.) in Bincau Village, Banjar Regency, South Kalimantan Manap Trianto; Fajri Marisa; Moh Dahri Kisman
Biota Vol 13 No 2 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i2.304

Abstract

Chili is a widely cultivated plant by the community because of its profit. Currently, in maintaining the quality of chili, people commonly use a lot of pesticides. In contrast, naturally, there are biological agents in the shape of natural enemy insects, more effective, and environmentally friendly. This study aims to determine the diversity, abundance, and distribution patterns of natural enemy insects in chili plantation (Capsicum annum L.) at Bincau Village, Martapura District, Banjar Regency, South Kalimantan. The sample is collected by pitfall trap, yellow pan trap, and sweep net method. Obtained data are analyzed by Shannon Wienner index (H'), Meyer abundance index, Morisita index (Id), and PCA method for environmental parameter analysis. The results showed the diversity of natural enemy insects on chili plants was moderate (H' = 2.396). Furthermore, the highest abundance in research is demonstrated on Hymenoptera order, which consisted of 9 families, 16 species, and 1,009 individuals. The distribution pattern of natural enemy insects in chili plantations is clustered (Id > 1). Based on the PCA analysis method, the temperature has the most impact on natural enemy insects' presence in this study.
Deteksi Awal Aktivitas Antibakteri dari bakteri air tambak terhadap bakteri patogen akuakultur Stella Magdalena; Sindy Sindy; Yogiara Yogiara
Biota Vol 13 No 2 (2020)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v13i2.305

Abstract

Aquaculture is currently experiencing massive loss due to the outbreak of pathogenic bacteria. One of the outbreak causes is the development of pathogenic bacterial resistance to the antibacterial. The problem can be solved using microorganisms that can produce new antibacterial compounds. The purpose of this research was to obtain bacteria from fishpond water that could produce antibacterial compounds. About two out of 81 isolates could produce antibacterial compounds. Those two isolates were obtained from saltwater fishponds in North Jakarta (TS2) and Harapan Island (PHY). All fishpond water was grown in marine broth or Luria broth. Extraction of antibacterial compounds was performed using four types of solvents: chloroform, dichloromethane, ethyl acetate, and methanol. Each of the solvents showed a different result. The extraction can only be successfully performed using chloroform and dichloromethane. Extraction using dichloromethane showed a larger inhibitory clear zone than chloroform. Based on 16S rRNA gene sequencing, PHY isolate was identified as Bacillus sp. and TS2 as Acinetobacter sp. In conclusion, isolate TS2 and PHY, which produced antibacterial compounds, showed potential use as aquaculture probiotics.

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