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INDONESIA
JFIOnline
Published by Ikatan Apoteker Indonesia
ISSN : 14121107     EISSN : 2355696X     DOI : -
Core Subject : Health,
Health Professions Medicine & Pharmacology
Jurnal Farmasi Indonesia yang diterbitkan oleh Pengurus Pusat Ikatan Apoteker Indonesia. Isi website memuat seluruh jurnal yang telah diterbitkan mencakup semua aspek dalam ilmu pengetahuan dan teknologi kefarmasian antara lain farmakologi, farmakognosi, fitokimia,farmasetika, kimia farmasi, biologi molekuler, bioteknologi, farmasi klinik,farmasi komunitas, farmasi pendidikan, dan lain-lain.
Arjuna Subject : -
Articles 443 Documents
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KERASIONALAN PENGGUNAAN ASIKLOVIR PADA SALAH SATU POLIKLINIK KULIT DAN KELAMIN DI SUMATERA SELATAN Suardi, Muslim; Erjon, .; Khodijah, .
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 3, No 4 (2007)
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The aim of this study was to observe the rationality of acyclovir use and to provide information in order to improve quality of medical services at a hospital in Sumatera Selatan. A descriptive survey studies was conducted on 77 patients Herpes simplex Virus (HSV), and Varicella zoster Virus (VZV) infections at Ambulatory Clinic of Dermato-Venereology. Data were collected from status cards in medical record installation using systematic sampling method. Data were analyzed quantitatively and qualitatively as well. Results showed that the score of rationalities in drug therapy regarding the appropriateness of indication, drug, and patients, were 100, 89.6, and 100% respectively. While rationalities regarding the appropriateness of: dose, route, time, duration, frequency, and interval of administrations were 49.4, 88.3, 0, 0, 72.7, and 72.7% respectively. As a conclusion, the usage of acyclovir at Ambulatory Clinic of Dermato-Venereology at a hospital in Sumatera Selatan Province hospital were irrational. ABSTRAK Penelitian ini bertujuan untuk mengetahui kerasionalan penggunaan ACV dan diharapkan sebagai bahan masukan untuk meningkatkan mutu pelayanan kesehatan di salah satu Rumah Sakit di Provinsi Sumatera Selatan. Satu rancangan penelitian survei deskriptif dilakukan terhadap 77 pasien infeksi Virus Herpes simpleks (VHS), dan Virus Varisela zoster (VVZ) yang berobat di Poliklinik Kulit dan Kelamin. Data diperoleh dari kartu status pasien pada instalasi rekam medik selama tahun 2005. Selanjutnya data ditabulasikan dalam tabel, dianalisis secara kuantitatif maupun kualitiatif dan dipaparkan dalam bentuk grafik. Hasil penelitian menunjukkan bahwa penilaian kerasionalan yang mencakup tepat indikasi, tepat obat, dan tepat penderita, masing-masing adalah, 100; 89,6; dan 100%. Sedangkan penilaian kerasionalan yang mencakup tepat dosis, tepat rute, tepat saat, tepat lama, tepat frekuensi, dan tepat interval pemberian masing-masing adalah: 49,4 ; 88,3; 0; 0; 72,7; dan 72,7%. Sebagai kesimpulan, penggunaan ACV pada Poliklinik Kulit dan Kelamin salah satu Rumah Sakit di Provinsi Sumatera Selatan adalah tidak rasional.
ISOLASI SENYAWA ALKALOID DARI TUMBUHAN Cryptocarya bracteolata (LAURACEAE) Saidi, Nurdin; Morita, Hiroshi; Litaudon, Marc; Mukhtar, Mat Ropi; Awang, Khalijah; Hadi, A. Hamid A.
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 1 (2012)
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Isolation of alkaloids from the bark of C. bracteolata Gamb yielded two compounds ofaporphines alkaloid, (+)-Lirioferine and (+)-Bracteoline. The extraction of the bark wascarried out by maceration, and isolation was carried out by column chromatography.Structural elucidation was established through several spectroscopic methods, such as 1DNMR(1H, 13C, DEPT), 2D-NMR (COSY, HMQC, HMBC), UV, IR, MS and comparison withthe published data.ABSTRAKDua senyawa alkaloid jenis aporfin, yaitu (+)-Lirioferin dan (+)-Bracteolin telah diisolasi darikulit batang C. Bracteolata. Ekstraksi senyawa dilakukan menggunakan cara maserasi danisolasi dengan kromatografi kolom. Elusidasi struktur dilakukan dengan berbagai jenisspektroskopi, meliputi 1D-NMR (1H, 13C, DEPT), 2D-NMR (COSY, HMQC, HMBC), UV, IR,EIMS dan perbandingan data senyawa yang sudah dipublikasi sebelumnya.
PENGEMBANGAN POTENSI RHODOMYRTONE SEBAGAI BAHAN AKTIF SEDIAAN TOPIKAL Fahmi, Rizal; Rullah, Kamal; Rahmat, Rosita Dewi; Lucida, Henny; Manjang, Yunazar; Hj. Lajisc, Nordin; Dachriyanus, .
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 1 (2012)
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Rhodomyrtone, an antibacterial constituent was isolated from the active fraction of theleaves extract of Karamuntiang, Rhodomyrtus tomentosa (Aiton) Hassk. In this presentstudy, the topical cream formula of rhodomyrtone 2% in vanishing cream was evaluated byin vitro and in vivo preclinical test. The in vitro test was peformed against Staphylococcusaureus ATTC 6538 and Staphylococcus epidermidis ATTC 12228 by using disk agardiffusion method, while the in vivo test was evaluated on the surface of skin infection area ofthe rabbit caused by Staphylococcus aureus. Both these tests were guided bychloromphenicol 2% cream as a comparison. The topical cream of rhodomyrtone 2% invanishing cream showed diameters of inhibition zones of 15 mm and 26 mm towardStaphylococcus aureus and Staphylococcus epidermidis respectively. These diffusions werelower than inhibition zones of chloromphenicol 2% cream which has 30 mm and 32 mm forthe same bacterias in the same condition. The in vivo test showed that rhodomyrtone 2%topical cream can reduced that skin infection and did not caused irritation on the testedrabbits.ABSTRAKRhodomyrtone adalah suatu konstituen antibakteri fraksi aktif ekstrak daun karamuntiang,Rhodomyrtus tomentosa (Ait) Hassk. Pada penelitian ini telah dilakukan uji preklinisterhadap krim sediaan topikal Rhodomyrtone 2% dalam vanishing cream. Uji preklinissecara in vitro menggunakan media agar dan Staphylococcus aureus ATTC 6538 danStaphylococcus epidermidis 12228 sebagai bakteri uji, sedangkan secara in-vivo dilakukanterhadap kulit pada kelinci yang di infeksi dengan Staphylococcus aureus. Kedua uji inimenggunakan krim kloromfenikol 2% sebagai pembanding. Krim sediaan topikalrhodomyrtone 2% dengan basis vanishing cream memberikan diameter hambatan berturutturut15 mm dan 26 mm terhadap Staphylococcus aureus dan Staphylococcus epidermidis.Difusi ini lebih rendah dibanding sediaan krim kloromfenikol 2% yang memberikan diameterhambatan 30 mm dan 32 mm, masing-masing untuk Staphylococcus aureus danStaphylococcus epidermidis pada kondisi yang sama. Selanjutnya uji secara in vivomenunjukkan bahwa krim sediaan topikal rhodomyrtone 2% dapat mengurangi infeksi kulitdan tidak menimbulkan iritasi pada kelinci percobaan.
OPTIMASI DAN VALIDASI METODE ANALISIS ASAM NIKOTINAT SERTA STABILITAS INOSITOL HEKSANIKOTINAT Harahap, Yahdiana; Suryadi, Herman; Wardatun, Sri
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 1 (2012)
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Nicotinic acid is a therapeutic agent for treatment atherosclerosis. Inositol hexanicotinate isan agent that can be hydrolyzed with release nicotinic acid. The low level of free nicotinicacid from inositol hexanicotinate in blood, it’s the reason why it needs method analysis withhigh sensitive and selective. The aims of this research were to optimize and validationmethod analysis nicotinic acid and stability study of inositol hexanicotinate by highperformance liquid chromatography. The method was optimated with variation compositionmobile phase, variation flow rate and optimation process exctraction. Condition analysiswere optimum with use a Kromasil column (250 mm x 4,6 mm) RP, mobile phase mixeddipotassium hydrogen phosphate and potassium dihydrogen phosphate 10 mM containingtetrabuthylammonium bromide 5 mM pH 7 with acetonitril (100:9), flow rate 0,8 ml/minute,with internal standard coffein in 263 nm wave lenght. The standard curve was linear over aconcentration range 124,84 to 5000 ng/ml of nicotinic acid in plasma. The HPLC method wasvalidated with accuracy -6,8779 to 3,09 %, precision 0,3 to 3,71 % and recovery 93,12 -103,09 %. The results of a stability study indicated that inositol hexanicotinate was unstablein plasma samples, but was stable in 0,6 M perchloric acid for to 24 hour at 40C. ABSTRAKAsam nikotinat merupakan obat yang dapat digunakan untuk ateroskerosis dan inositolheksanikotinat merupakan senyawa yang dapat terhidrolisis melepaskan asam nikotinat.Penelitian ini bertujuan untuk optimasi dan validasi metode analisis asam nikotinat, sertamenentukan stabilitas inositol heksanikotinat menggunakan kromatografi cair kinerja tinggi.Kondisi analisis yang optimum diperoleh dengan menggunakan kolom Kromasil (250 mm x4,6 mm) RP, fase gerak campuran kalium dihidrogen fosfat dan dikalium hidrogen fosfat 10mM yang mengandung tetrabutilammonium bromida 5 mM pH 7 dengan asetonitril (100:9),kecepatan alir 0,8 ml/menit, dengan baku dalam kafein, yang dideteksi pada panjanggelombang 263 nm. Kurva kalibrasi linier dari 124,84 sampai 5000 ng/ml. Hasil validasimetode menunjukkan akurasi -6,87 hingga 3,09 %, presisi 0,3 hingga 3,71 % dan perolehankembali 93,12 hingga 103,09%. Hasil uji stabilitas menunjukkan bahwa inositolheksanikotinat tidak stabil dalam plasma tetapi stabil dalam asam perklorat 0,6 M padapenyimpanan 40C selama 24 jam.
OPTIMASI ASAM OLEAT, PROPILEN GLIKOL DAN IONTOFORESIS TERHADAP TRANSPOR TRANSDERMAL PROPRANOLOL HCL Hendriati, Lucia; Nugroho, Akhmad Kharis
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 1 (2012)
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The purposes of this study were to evaluate the influences of oleic acid, propylene glycol andiontophoretic on the transport of propranolol HCl and to know optimum formula. Eightconditions were prepared according to 23 factorial design emplyoing oleic acid, propyleneglycol, and iontophoretic. Enhancement effect on transport of propranolol HCl wasinvestigated across hairless rat skin. The study was carried out by using vertical diffusioncell. Transport of propranolol HCl across membrane was determinedspectrophotometrically. Data were analysed based on compartment modeling to determinetransport kinetics and then the optimum formula was proposed. The transport kinetics ofpropranolol HCl followed first order. Oleic acid and propylene glycol increased the potentialof the drug to difuse (AD) but had no effect on drug release rate from donor compartement tomembrane (Ka) and from membrane to acceptor compartment (KR). Iontophoretic increasedKa and decreased KR. Based on values of Ka, AD, KR, optimum formula containing oleicacid, propylene glycol and iontophoretic which have the percentage and current density of4,8%, 20% and 0,11 mA/cm2, respectively.ABSTRAKPenelitian ini bertujuan mengetahui pengaruh asam oleat, propilen glikol dan iontoforesisterhadap parameter transpor transdermal propranolol HCl dan mengetahui komposisiformula optimum. Delapan formula disusun berdasarkan desain faktorial 23 denganmenggunakan pemacu transpor asam oleat, propilen glikol dan iontoforesis. Pengujianpenetrasi dilakukan secara in vitro menggunakan sel difusi. Kulit tikus sebagai membranmengalami praperlakuan dengan asam oleat dan propilen glikol, diikuti dengan iontoforesis.Jumlah propranolol HCl yang tertranspor ditetapkan dengan metode spektrofotometri.Analisis data menggunakan modeling berbasis kompartemen dilanjutkan dengan pemilihanformula optimal. Transpor propranolol HCl dijelaskan melalui model tiga kompartemen.Asam oleat dan propilen glikol meningkatkan potensi obat tertranspor (AD), namun tidakmempengaruhi kecepatan absorbsi dari kompartemen donor ke kulit (Ka) maupunkecepatan pelepasan dari kulit ke kompartemen reseptor (KR). Arus iontoforesismeningkatkan harga Ka dan menurunkan KR. Berdasarkan harga parameter Ka, AD dan KR,komposisi formula optimum adalah asam oleat 4,8%, propilen glikol 20% dan iontoforesis0,11% mA/cm2.
PERBANDINGAN PENGGUNAAN OBAT ANTARA PASIEN PNEUMONIA RAWAT INAP DI RUMAH SAKIT Rais, Yulia; Trisna, Yulia; Andrajati, Retnosari
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 1 (2012)
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Pneumonia is the third major disease suffered by inpatient class III in RSUPN Dr. CiptoMangunkusumo Jakarta, among other are patient of Jamkesmas, Askessos and Gakin.Treatment expense for patient Jamkesmas based on the tariff packet of INA-DRG.Thepurposes of this study were to compare of drug use by ATC/DDD methode and comparedthe portion of the cost for drugs and health supplies to INA-DRG Tariff, among the thirdpatient group. Design of this study was cross sectional. Data were collected retrospectivelyfrom medical record and prescriptions for inpatient of pneumonia with Jamkesmas,Askessos, and Gakin Program at Cipto Mangunkusumo Hospital Jakarta, over period ofMarch 2008 – March 2009. The result of this study showed that quantity of drug use atJamkesmas, Askessos and Gakin successively were differed, successively 391,500;380,211; and 741,895 DDD/hbd. Generic name in DU90% at Jamkesmas, Askessos andGakin were also significantly differed, i.e. 22 (31%), 19 (24%), 37 items (40%). There wasalso a significant difference among the three health insurances in regards with the portion ofthe cost for drugs and health supplies to INA-DRG Tariff.ABSTRAKPneumonia adalah penyakit ketiga terbanyak yang diderita oleh pasien di ruang rawat inapkelas III di RSUPN Dr. Cipto Mangunkusumo Jakarta, diantaranya adalah pasienJamkesmas, Askessos dan Gakin. Biaya perawatan bagi pasien Jamkesmas dilakukanberdasarkan Tarif paket INA-DRG. Penelitian bertujuan untuk membandingkan penggunaanobat dengan Metode ATC/DDD dan membandingkan persentase biaya obat dan alatkesehatan terhadap Tarif INA-DRG antara ketiga kelompok pasien. Penelitian dilakukandengan rancangan studi potong lintang. Data diambil secara retrospektif dari rekam medikdan resep dokter untuk pasien pneumonia program Jamkesmas, Askessos dan Gakin,periode Maret 2008 – Maret 2009. Hasil penelitian menunjukkan kuantitas penggunaan obatpada pasien Jamkesmas, Askessos dan Gakin berbeda bermakna, berturut-turut adalah391,500; 380,211; 741,895 DDD/shr. Obat dengan nama generik dalam segmen DU90%pada pasien Jamkesmas, Askessos dan Gakin berturut-turut juga brbeda bermakna,berturut-turut 22 item (31%), 19 item (24%), 37 item (40%). Juga terdapat terdapatperbedaan yang bermakna dalam persentase biaya obat dan alat kesehatan terhadap TarifINA-DRG antara ketiga kelompok yang dibandingkan.
PENGETAHUAN PASIEN TENTANG DIABETES DAN OBAT ANTIDIABETES ORAL Nita, Yunita; Yuda, Ana; Nugraheni, Gesnita
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 1 (2012)
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The objective of this work was to determine patient knowledge regarding diabetes mellitusand oral antidiabetics drugs (OAD). Six pharmacies in Surabaya were chosen purposively inAugust 2009. The sample was DM patients who obtained OAD with prescription. Interviewswere conducted using validated questionnaires. Result showed that 95.8% (69/72) ofpatients knew the aim of DM therapy. More than 90% of patients knew that medicine,exercise and diet are the therapy for DM. The correct time of taking the medication wasknown by 57.9% (22), 43.3% (13) and 0% of patients who received 1, 2 and 3 OADrespectively. A total of 64 patients received insulin secretagogues or sulfonylureas whichhave side effects of hypoglycemia. Only 9.5% (6) of patients knew the definition ofhypoglycemia, and less than 21% of patients knew the signs of hypoglycemia. If forget totake medication, 95.8% (69/72) of patients knew that the OAD should not be taken double.To conclude, patients’ knowledge about diabetes and OAD must be improved. ABSTRAKTujuan penelitian ini adalah untuk mengetahui pengetahuan pasien Diabetes Mellitustentang obat antidiabetes oral (OAD). Dilakukan di 7 apotek di Surabaya secara purposivesampling pada bulan Agustus 2009. Sampel adalah pasien DM yang menebus OAD denganresep di apotek terpilih. Data diperoleh dari interview menggunakan daftar pertanyaanterstruktur yang telah divalidasi. Diperoleh 72 pasien sebagai responden dari penelitian ini.Dari hasil penelitian diperoleh 95,8% (69) responden mengetahui tujuan terapi DM. Lebihdari 90% responden mengetahui bahwa OAD, olah raga dan pengaturan diet adalah terapiuntuk DM. Waktu yang benar dalam menggunakan obat diketahui oleh 57.9% (22), 43.3%(13) dan 0% responden yang mendapat 1, 2 dan 3 OAD. Sejumlah 64 respondenmemperoleh golongan insulin secretagogue atau sulfonylurea yang memiliki efek sampinghipoglikemia. Hanya 9.5% (6) responden yang mengetahui definisi hipoglikemia, dan kurangdari 21% mengetahui tanda-tanda hipoglikemia. Sementara 70,8 % (51/72) mengetahuibahwa apabila mereka mengalami lemas, berkeringat dan akan pingsan sebaiknyamengkonsumsi gula. Dapat disimpulkan bahwa pengetahuan pasien tentang DM dan OADmasih harus ditingkatkan.
IMPLEMENTASI CARA DISTRIBUSI OBAT YANG BAIK PADA PEDAGANG BESAR FARMASI DI YOGYAKARTA Putra, Anthonius Ade Purnama; Hartini, Yustina Sri
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 1 (2012)
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Government Regulation of The Republic of Indonesia number 51/ 2009 stated thatPharmacist must be the person that responsible for pharmaceutical wholesaler activity.Pharmaceutical wholesaler must implement Good Distribution Practices (GDP). There are79.045 types of registered pharmaceuticals that distributed by 2.821 pharmaceuticalwholesalers in 33 Indonesia’s provinces. This research aimed to evaluate theimplementation of GDP on pharmaceutical wholesalers in Daerah Istimewa Yogyakarta(DIY) Province. This research conducted in July 2010 using questionnaire and interview to29 pharmaceutical wholesaler are willing to become respondents from 49 pharmaceuticalwholesaler listed in DIY. The products distributed by pharmaceutical wholesaler in DIYProvince which is the raw material of pharmaceuticals, vaccines, psychotropic, prescriptiondrugs, over the counter drugs, cosmetics, food, milk, and medical equipment. Theresponsible person in 83% pharmaceutical wholesaler is a woman, 38% chargepharmaceutical wholesaler 23-30 years old, pharmacist being the responsible person on31% pharmaceutical wholesaler, 52% had never been in charge GDP training. There are 3%pharmaceutical wholesaler which doesn’t have a Standard Operating Procedure, 21% don’thave the organizational structure, 59% didn’t have temperature control equipment, 34% don’thave humidity control equipment, 3% don’t carry out the documentation, and 10% didn’tconduct self inspections. ABSTRAKTerdapat 79.045 jenis sediaan farmasi yang berizin edar yang didistribusikan oleh 2.821PBF yang tersebar di 33 provinsi di Indonesia. Penelitian ini mengevaluasi implementasiCDOB pada PBF di Provinsi Daerah Istimewa Yogyakarta (DIY). Survei dilakukan bulan Juli2010 menggunakan kuesioner dan interview kepada 29 PBF yang bersedia menjadiresponden dari 49 PBF yang tercatat di Propinsi DIY. Hasil penelitian menunjukkan bahwaproduk yang disalurkan oleh PBF di Provinsi DIY yakni bahan baku farmasi, vaksin,psikotropik, obat keras, obat bebas, obat bebas terbatas, kosmetik, makanan, susu, danalat kesehatan. Sebanyak 83% PBF, penanggung jawabnya wanita, 38% penanggung jawabPBF berumur 23-30 tahun, 31% penanggung jawab PBF adalah apoteker, 52% penanggungjawab PBF belum pernah mengikuti pelatihan CDOB. Terdapat 3% PBF yang tidak memilikiStandar Operasional Prosedur, 21% tidak memiliki struktur organisasi, 59% tidak memilikialat pengontrol suhu, 34% tidak memiliki alat pengontrol kelembaban, 3% tidakmelaksanakan dokumentasi, dan 10% tidak melakukan inspeksi diri.
Pengaruh Vanadil Sulfat Terhadap Ekspresi Protein GLUT-4 pada Mencit yang Menderita Diabetes Mellitus Holidah, Diana; Khotib, Junaidi
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 2 (2012)
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The Present study was designet to investigate the influence of vanadyl sulphate towards GLUT-4 protein activities in skeletal muscle tissue in streptozotocin induced diabetic mice. Twenty five mice divided into five groups i.e. placebo group, diabetic group, and three treatment groups based on vanadyl's doses (5,30 or 100 mg/kg BW, respectively). Diabetic mice model was induced by twice intraperitoneal administration of streptozotocin. The first dose of streptozotocin is 100 mg/kg that were inject in the first day and then dose 50 mg/kg, in the day 14th. Diabetes occured on 21st day after streptozotocin injection and it was shown by increasing blood glucose level from 151.4 ± 25.1 mg/dL to 237.1 ± 33.0 mg/dL. Administration of vanadyl sulphate at the dose of 5,30 or 100 mg/kg BW was significantly reduces blood glucose concentration (p<0,001). The muscular tissue were harvested on the day 28th and stained using routine histology staining, hematoxylin-eosin for morphological qualitative analysis and immunohistochemical examination to observe the activities of GLUT-4 protein in skeletal muscle. The result showed that vanadyl sulphate restore atrophic condition of muscular cells and inhibit cell necrosis in muscular tissue. On immunohistochemical examination, vanadyl sulphate might increased the GLUT-4 protein activities in skeletal muscle in streptozotocin-induced diabetic mice Keywords : vanadyl sulphate, streptozotocin, diabetes mellitus, GLUT-4 Abstrak Penelitian ini bertujuan untuk mengetahui pengaruh vanadil sulfat terhadap aktivitas protein GLUT-4 pada otot skelet mencit yang menderita diabetes mellitus akibat induksi streptozotocin. Sebanyak 25 ekor mencit dibagi menjadi 5 kelompok, yaitu kelompok kontrol, diabtes dan perlakuan yang diberi vanadil sulfat dengan dosis 5,30 dan 100 mg/kgBB. Keadaan diabetes diinduksi dengan pemberian streptozotocin dosis 100 mg/kgBB pada hari pertama dan 50 mg/kgBB pada hari ke -14. Pada hari ke-21 terjadi peningkatan kadar glukosa dari 151,4 ± 25,1 mg/dL menjadi 237,1 ± 33,0 mg/dL. Pemberian vanadil sulfat selama 7 hari akan menurunkan kadar glukosa darah secara signifikan (p<0,001). Otot skelet diambil pada hari ke-28 dan dipreparasi dengan hematoksilin-eosin untuk diamati histologinya dan secara imunohistokimia untuk pengamatan aktivitas protein GLUT-4. Hasil pengamatan menunjukkan pemberian vanadil sulfat akan memperbaiki keadaan atropi dan menghambat nekrosis pada sel otot skelet. Pengamatan secara imonohistokimia menunjukkan bahwa vanadil sulfat dapat meningkatkan aktivitas GLUT-4. Kata Kunci: vanadil sulfat, streptozotocin, diabetes mellitus, GLUT-4
Antiproliferative Activities of Dianella nemorosa Lam. Leaves Methanol Extract Against HCT-116, C2C12 and 293A Cell lines Karim, Aditya Krishar; Asmara, Widya; Sismindari, .; Istriyati, .; Nohno, Tsutomu
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 2 (2012)
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Dianella nemorosa Lam. (Liliaceae) is a medicinal plant traditionally used in Papua for treatment of cancer, injury, fracture, inflammation and also for antiseptic. The aim of this study was to evaluate antipriliferative activities of methanol extract of D. nemorosa leaves against HCT+116 (colorectal cell line), C2C12 (adherent mouse myoblast cell line) and 293A (Primary embryonal human kidney transformed with human adenovirus type 5 DNA). Powder of leaves was extracted using methanol. Antiproliverative activities were determined by cell proliferation reagents WST-1 assay for 1 h, 2h and 4h after 72h incubation. The result showed that methanol extract of D.nemorosa leaves showed remarkable antiproliferatiove activities against HTC-116 cell line with IC50 values of 199.31 μg/ml (1h), 197.87 μg/ml (2h) and 161.12 μg/ml (4h). The activities against C2C12 cell line resulted in the IC50 values of 405.51 μg/ml (1h), 435.12 μg/ml (2h) and 394.38 μg/ml (4h), while the IC50 values for 293A cell line were 580.81 μg/ml (1h), 442.21 μg/ml (2h) and 366.74 μg/ml (4h), respectively. Those results indicated that methanol extract of D.nemorosa leaves posses potential antiproliferative activities against HCT-116, C2C12 and 293A cell line. Further study is necessary to investigate the inhibitory mechanism of methanol extract of D. nemorosa leaves on HCT-116, C2C12 and 293A cell lines.Keywords : Dianella nemorosa Lam, cancer cell, HCT-116, C2C12, 293A

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