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Achmad Arifiyanto
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Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Published by Universitas Lampung
ISSN : 23384344     EISSN : 2686200X     DOI : https://doi.org/10.23960/jbekh.vxxx.xxx
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
This journal focuses on the fields of Agricultural, Animal Sciences, Bioconservation, Biopharmacology, Biotechnology, Biomedical, Biological control, Behavioural ecology, Plant Sciences, Ecology, Fishery, Marine Biology, Fresh Water Biology, Microbiology, Immunobiology, Ecotoxicology, and Parasitology. This journal utilizes both the LOCKSS and CLOCKSS systems to create a distributed archiving system among participating libraries and permits those libraries to create permanent archives of the journal for purposes of preservation and restoration
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 10 Documents
 1 
Search results for , issue "Vol. 11 No. 2 (2024)" : 10 Documents clear
The Effect of The Tree Rats (Rattus Tiomanicus (Miller, 1900)) Attack on The Quality of The Crude Palm Oil (CPO) Palm Oil (Elaeis Guineensis Jacq.) Plantations of Palangkaraya Central Kalimantan Dasuki, Diva Rosa Nirwana; Nurcahyani, Nuning; Mumtazah, Dzul Fithria; Master, Jani
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.369

Abstract

Indonesia is the world’s largest producer of Crude Palm Oil (CPO). Tree rats (Rattus tiomanicus (Miller, 1900)) pose a significant problem in palm oil plantations by eating and damaging the mesocarp, reducing fruit weight, and affecting the Oil Extraction Rate (OER) and Free Fatty Acid (FFA) levels in CPO. This study investigates the impact of rat bites on CPO quality, focusing on OER and FFA levels. Using an experimental method, palm fruit groups with varying rat attack levels were compared to rested (3 days delayed in transport) and other pest attacked fruit groups. Results from a one-way ANOVA and Post Hoc LSD test showed that severe rat attacks (>50%) significantly affected OER and FFA compared to other conditions.
Activity of Cellulolytic Bacteria Origin From Sediment of Mangrove Forest Ecotourism Pesawaran Lampung Chusniasih, Dewi; Handayani, Catie; Istiadi, Khaerunissa Anbar
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.371

Abstract

Cellulolytic bacteria can hydrolyze substrates containing cellulose such as mangrove litter trapped in mangrove sediment by producing cellulase enzymes to break down cellulose into simpler compounds. The purpose of this study is to obtain bacterial isolates from mangrove sediment that have the ability to produce cellulase enzymes and to determine the activity of the cellulase enzymes they produce in degrading cellulose substrates. The methods used in this study include isolation of cellulolytic bacteria from mangrove sediments, qualitative enzyme activity test, standard curve of bacterial growth, bacterial growth curve for 24 hours, standard glucose curve, and quantitative enzyme activity test using a spectrophotometer. From the isolation results obtained 18 candidate cellulolytic bacteria with different macroscopic and microscopic characteristics. Based on the results of the qualitative enzyme test for 4 days on Zobell media added with 0.5% CMC, enzyme activity index was obtained from 5 isolates. The highest activity index of isolate M2 was on day 3 that was 1.96, isolate M3 had the highest activity index on day 4 that was 4.29, isolate M4 was 2.02 on day 4, isolate M6 was 4.39 on day 4, and isolate M13 was 3.87 on day 4. Furthermore, 3 potential iso-lates were selected based on the highest average index of enzyme activity for quantitative tests. The quantitative test results from the crude extract of the cellulase enzyme for 7 days on 0.5% CMC substrate showed that the highest hydrolysis activity occurred on day 5 by isolate M6, that was 0.151 U/mL. The other two cellulolytic isolates, isolate M3 and isolate M13, had the highest cellulose hydrolysis activity, 0.129 U/mL and 0.137 U/mL.
Increase in Neutrophil Lymphocyte Ratio in COVID-19 Patients Based on Symptom Severity Prasetya, Hieronymus Rayi; Muhajir, Nurlaili Farida; Wati, Mey Rena; Murtiningrum, Sri
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.374

Abstract

The neutrophil lymphocyte ratio (NLR) is an indicator of the presence of a systemic inflammatory response. The neutrophil lymphocyte ratio parameter was calculated by comparing the number of neutrophils and the number of lymphocytes. Patients with severe COVID-19 who did not survive were found to have a higher neutrophil lymphocyte ratio compared to patients with a mild prognosis. Thisstudy uses a descriptive method, which aims to describe the value of the neutrophil lymphocyte ratio in COVID-19 patients based on the severity of symptoms. The average value of high neutrophil lymphocyte ratio (NLR) in COVID-19 patients occurred in the severe (6.12%) and critical (10.78%) categories. The average value of high neutrophils (Neutrophilia) in COVID-19 patients occurred in the severe (76%) and critical (82%) categories. The average value of low lymphocytes (lymphocytopenia) in COVID-19 patients occurred in the severe 17% and critical 12% category. The NLR value in patients with the severe category had an increase in the NLR value twice thenormal value, while in the critical category had an increase in the NLR value three times the normal value. 
Analysis of Microplastic at Sea Water and Sediment in the Pasaran Island Bay Using FT-IR Pamungkas, Galuh Rara; Widiastuti, Endang Linirin; Pratami, Gina Dania; Tugiyono, Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.378

Abstract

Marine pollution from ocean garbage, particularly plastic waste, has significant ecological impacts globally. Plastic debris dominates marine pollution and graadually breaks down into microplastics. This study analyzes microplastic particle count, shapes, and polymer types in water and sediiment around Pasaran Island. Research was conducted across four stations (Station I, II, III, and IV).  Microplastics in seawater samples were extracted using solutions of 70% ethanol, 30% H2O2, and 30% NaCl. Sediments were treated with FeSO4 (0.05 M), NaCl, and 30% H2O2. Particle counts and shapes were identified with a digital microscope and polymer types were determined via Fourier Transform Infrared Spectroscopy (FT-IR). Microplastic forms observed included fibers, fragments, film, and pellets, with fibers being the most abundant and pellets the least. Detected polymers included polyamide (PA), polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polystyrene (PS), and polytetrafluoroethylene (PTFE), originating from both local plasstic use and seawater flow around Pasaran Island.
Meta Analysis: The Soil Transmitted Helminth Infection and It’s Correlation with Anemia in Children Nuryati, Arum
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.381

Abstract

Soil-transmitted helminth (STH) infections are common in regions with high rates of anemia, particularly in low-income countries where poor nutrition is prevalent. Children attending school in these areas are most vulnerable to anemia. Both anemia and STH infections can negatively affect children's physical development, school attendance, and learning abilities. This study aims to examine the relationship between STH infections and anemia in children. A systematic review and meta-analysis following PRISMA guidelines were conducted. Articles were selected based on eligibility criteria defined using the PICO model: Population = Primary School Children, Intervention = STH infections, Comparison = No STH infections, Outcome = Anemia. The article search covered publications from 2002 to 2024 across databases such as PubMed, Science Direct, Google Scholar, Springer Link, Hindawi, and PLOS One. Keywords used included "STH," "Anaemia," "STH AND Children," and "STH AND Anaemia." Inclusion criteria consisted of full-text, cross-sectional studies published in English that discussed the impact of STH on anemia in school-aged children. The final results were expressed as adjusted odds ratios (aOR) from multivariate analysis. Data were analyzed using RevMan 5.4, and meta-analysis was performed on 12 articles from Africa and Asia. The results indicated that children infected with STH had significantly lower hemoglobin levels, leading to anemia (aOR = 2.23, 95% CI = 1.07 to 4.65, p = 0.03). This study demonstrates that STH infections contribute to anemia in school-aged children.
The Effect of Black Pepper (Piper nigrum L.) Extract on Morphology and number of Mice (Mus musculus L.) Spermatozoa Induced by Progestin Hormone Indriyani; Sutyarso; Busman, Hendri; Nurcahyani, Nuning; Setyaningrum, Endah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.384

Abstract

The progestin hormone is used as male hormonal contraception. Giving progestin to normal men can  suppress libido and testicular function effectively, and reduce testosterone levels so that it can interferer with the spermatogenesis process. This study is about the effectiveness of black pepper (Piper nigrum L.) extract on morphology and number of spermatozoa in mice (Mus musculus L.)  induced by progestin. This study used a completely randomized design (CRD) which has divided into four treatment groups, each group contained 5 mice and treated according to the experimental design. Group P0 as a control (given H2O), P1 (induced by 1,25 mg progestin), P2 (induced by 1,25 mg progestin and 3.33mg/Kg BW ethanol extract of black pepper), and P3 (induced by 1,25 mg progestin and 3.33mg/Kg BW distilled water extract of black pepper). The Progestin hormone is given twice (week 1 and week 2). Black pepper extract was given daily for 35 days. Data were analyzed by one-way ANOVA and continued with the LSD test with a level of 5%. The result showed that giving of ethanol extract and distilled water extract from black pepper could improve the normal morphology and number of spermatozoa in mice after induced by progestin hormone so that fertility in men increases.
Insect Diversity in Organic Rice Field Areas in Sumberjambe: Keanekaragaman Serangga Pada Area Sawah Organik Di Sumberjambe Awaluddin, Ahmad Rusdy; Priantari, Ika; Afrida, Indah Rachmawati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.385

Abstract

This study aimed to analyze insect diversity in organic rice fields planted with white and black rice varieties. Yellow pan traps were used to collect insects, with six traps placed in each field. The traps, consisting of 12 cm yellow bowls filled with a 70% alcohol-soap solution, were mounted on 30 cm bamboo sticks and secured with double-sided tape. Sampling was conducted over nine weeks at three intervals (weeks 3, 6, and 9), with three replicates per week. Collected insects were carefully removed using a brush, preserved in bottles containing 70% alcohol, and labeled for plot identification. In the first week, insects in white rice fields included Asilidae, Tachinidae, Chrysomelidae, Syrphidae, and Tipulidae, while black rice fields contained Asilidae, Tachinidae, Sphecidae, Ichneumonidae, Chrysomelidae, and Coccinellidae. By the second week, white rice fields had Tachinidae, Asilidae, Sphecidae, Chrysomelidae, and Hesperiidae, while black rice fields had Tachinidae, Hesperiidae, Ichneumonidae, and Chrysomelidae. In the third week, white rice fields hosted Tachinidae, Ichneumonidae, Hesperiidae, Sphecidae, Coccinellidae, and Chrysomelidae, whereas black rice fields included Tachinidae, Halictidae, and Sphecidae.
Exploration and Identification of Trichoderma sp. with Baiting Method as a Biological Agent in Horticultural Lands Firdaus, Emilda Zahrotul; Indahsari, Wiwin Misnati Nur Indahsari; Bahri, Saiful; Tyastirin, Esti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.386

Abstract

The use of biological agents is an effective way to prevent the negative impacts of excessive synthetic chemical pesticide use. Trichoderma sp., a saprophytic fungus, acts as a biocontrol agent against various phytopathogenic molds and is more commonly found in rooted soil and leaves than in stems or fruits. This study aims to identify the characteristics and presence of Trichoderma sp. in horticultural land using the baiting method. Isolation was carried out by collecting fungi grown on rice, corn, and coconut bait using an inoculation needle. Three Trichoderma sp. isolates were obtained from chili and bamboo root soil using rice and corn bait. The results showed that the three isolates had similar characteristics: colonies were dark green, circular in shape, powdery in texture, and had entire margins. Microscopically, the isolates featured branched and upright conidiophores arranged vertically, short and thick phialides, round greenish conidia, and septate hyphae resembling threads.
Eco-enzyme from Orange Peel as a Key Ingredient in Anti Acne Peel-off Gel Mask Zummah, Atiqoh; Bahri, Saiful; Violando, Wiga Alif; Tyastirin, Esti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.387

Abstract

This study aims to develop a peel-off gel mask formulated from orange peel eco-enzyme solution and its physical characteristics and antibacterial activity against Staphylococcus epidermidis. The resulting gel mask has organoleptic characteristics of transparent brownish color, thick gel consistency, and fresh sour-sweet aroma. Physical characteristic tests showed that the gel mask meets pharmaceutical requirements, namely: pH value 4.5-6.5, spreadability 5-7 cm, and drying time 15-30 minutes. The mask showed good homogeneity without aggregates, although it was a little difficult to peel off, but it was not easily torn, and had stable thermodynamic stability. Antibacterial tests revealed that the peel-off gel mask made from orange peel eco-enzyme was effective in inhibiting the growth of Staphylococcus epidermidis. The diameters of the inhibition zones of the P1, P2, and P3 masks were 2.5 ± 0.5 mm, 2.1 ± 0.35 mm, and 1.5 ± 0.5 mm, respectively. These results indicate the potential of eco-enzymes from orange peel as active ingredients for peel-off gel mask formulation for skin care, especially in inhibiting acne-causing bacteria.
Biological Analysis of Mackerel Tuna (Euthynnus affinis) in Banda Sea Waters Ahmad, Sitti Wirdhana; Amirullah, Amirullah; Nur, Andi Irwan; Amalia, Kartika; Nasaruddin, Nasaruddin; Izal, Izal; Rudia, Adi Parman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.388

Abstract

Mackerel tuna (Euthynnus affinis) is a significant fishery commodity in the Banda Sea, part of Fisheries Management Area 714 (WPP 714). This study focused on analyzing the biological characteristics of mackerel tuna, including length-frequency distribution, recruitment patterns, growth parameters, and mortality rates. Research was conducted at the Kendari Ocean Fisheries Port (PPS), with catch data from the Banda Sea provided by PPS Kendari observers. Data were analyzed using Microsoft Excel and software applying Von Bertalanffy's growth model. Fish length increased throughout the year, with the highest frequency observed from April to May and an average length of 50 cm. Recruitment patterns revealed two annual peaks in March and August. The growth constant (K) was 0.632, with a growth index of 1.0. Mortality analysis showed total mortality (Z) at 6.70, natural mortality (M) at 1.24, fishing mortality (F) at 5.46, and an exploitation rate (E) of 0.82. These results suggest that while growth rates remain favorable, mackerel tuna is experiencing overexploitation, highlighting the need for sustainable fisheries management.

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