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Achmad Arifiyanto
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Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)
Published by Universitas Lampung
ISSN : 23384344     EISSN : 2686200X     DOI : https://doi.org/10.23960/jbekh.vxxx.xxx
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
This journal focuses on the fields of Agricultural, Animal Sciences, Bioconservation, Biopharmacology, Biotechnology, Biomedical, Biological control, Behavioural ecology, Plant Sciences, Ecology, Fishery, Marine Biology, Fresh Water Biology, Microbiology, Immunobiology, Ecotoxicology, and Parasitology. This journal utilizes both the LOCKSS and CLOCKSS systems to create a distributed archiving system among participating libraries and permits those libraries to create permanent archives of the journal for purposes of preservation and restoration
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 223 Documents
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Plankton Community Structure as a Bioindicator of Water Quality in the Way Sekampung River- the Segment of Rulung Helok Tugiyono, Tugiyono; Ayuni, Ubaid Jan; Priyambodo, Priyambodo; Susanto, Gregorius Nugroho
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 2 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i2.293

Abstract

Planktons are microorganisms that live in aquatic habitats. Plankton is used as a quality bioindicator of a water body. Way Sekampung flows to several areas in Lampung Province. The existence of activities around the watershed such as settlements, agriculture, and industrial waste disposal is thought to affect the water quality of the Way Sekampung River. The purpose of this study was to determine the water quality of Way Sekampung River based on plankton community structure consisting of abundance index, diversity index, uniformity index, and dominance index. Water sample data were analyzed using chemical parameters namely pH, BOD, DO, Phosphate, Nitrate, and physical parameters namely temperature and Total Suspended Solid (TSS) which are used to determine the status of water quality standards. This research was conducted at the beginning of the dry season (April) and the beginning of the rainy season (October). Sampling used a survey method based on the Way Sekampung River segment. Sampling points were carried out before and after the Argoguruh Dam. The results showed that the water quality of Way Sekampung River at the beginning of the dry season (April) and the beginning of the rainy season (October) were moderately polluted based on the analysis of plankton community structure such as abundance index, diversity, uniformity, and dominance.
Size Shortening of Body Parts of Fetus Mice (Mus musculus L.) After Giving Bitter Melon Fruit Extract (Momordica charantia L.) Andriani, Silvia; Nurcahyani, Nuning; Busman, Hendri; Sutyarso; Kurniawaty, Evi; Sahroni, Mizan; Puspa, Egita Windrianatama
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 2 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i2.298

Abstract

Bitter melon (Momordica charantia L.) is a plant that has traditional medicinal properties. In bitter melon fruit, there are several toxic compounds, namely momordicoside K and L, which are thought to have cytotoxic properties. This study aims to determine the teratogenic effect of bitter melon fruit extract given to pregnant females on the length of the fetus' cranium and sternum. Bitter melon fruit extraction was carried out using the maceration method using 95% ethanol. By using a completely randomized design 20 male mice and 20 female mice were divided into 4 groups. Each group consisting of 5 pregnant female mice was given bitter melon extract orally starting from day 6 to day 17 of pregnancy once a day in the morning with a treatment dose of aquabidest as a control [A], [B] 22.5 mg/30 gr BW, [C] 30 mg/30 gr BW, and [D] 37.5 mg/30 gr BW. Next, the female mice were dissected, the fetus was removed and the length of the cranium and the length of the sternum were measured. The results showed that in general, the administration of bitter melon fruit extract caused a decrease in the average length of the cranium and sternum. The results of the further analysis with the Least Significant Difference (LSD) at the 5% level showed that administering doses of [B], [C], and [D] had a significant effect on reducing the length of the cranium and sternum. The most effective dose to reduce the length of the cranium and sternum of fetal mice is 37.5 mg/30 g BW
Analysis of Taurine Content in Microalgae Tetraselmis sp. Cultured at Different Salinities Nurafiah, Eva; Widiastuti, Endang Linirin; Maharani, Henni Wijayanti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 2 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i2.304

Abstract

Taurine is a non-essential sulfur-containing amino acid with the chemical formula C2H7NO3S. These compounds play an important role in maintaining the smooth running of various processes in the body. Tetraselmis sp. is one of the microalgae known to have a taurine biosynthetic pathway via the serine/sulfate pathway. The purpose of this study was to determine the effect of culture salinity on taurine content produced by microalgae Tetraselmis sp. which was dissolved using 96% ethanol. The samples obtained were then tested using UV-Vis spectrophotometry using standard synthetic taurine as a comparison. Based on the research results, the maximum absorption was obtained at a wavelength of 630 nm, and the regression equation y = 0.001x + 0.033 was obtained and had a correlation coefficient of r = 1. Based on the analysis results showed that all samples contained taurine. Samples at a salinity of 25 ppt contained an average of 2.82 ml/100g of taurine, while a culture salinity of 35 ppt contained an average of 4.03 ml/100g of taurine. From these data it is known that the highest taurine level was found at a culture salinity of 35 ppt, while the lowest taurine level was at a culture salinity of 25 ppt. This indicates that the higher the culture salinity, the higher the taurine content produced by the microalgae Tetraselmis sp. The phenomenon of increased taurine due to salinity stress is thought to be due to an increase in free amino acids in microalgae cells to produce metabolites that are used to defend themselves due to salinity stress. The function of taurine in this microalgae is thought to be an organic osmolyte in the cells of the Tetraselmis sp. microalgae.
Differences in Soaking Time of Sea Urchin Gonad Extract (Diadema setosum) on Nile Tilapia (Oreochromis niloticus) Masculinization with a Dose of 4 mg/L : Perbedaan Lama Perendaman Ekstrak Gonad Bulu Babi (Diadema Setosum) Terhadap Maskulinisasi Ikan Nila (Oreochromis Niloticus) Dengan Dosis 4 Mg/L Sari, Mutiara Pradita; Susanto, Gregorius Nugroho; Widiastuti, Endang Linirin; Tugiyono, Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 2 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i2.310

Abstract

Nile tilapia (Oreochromis niloticus) is a fish that is in demand because of its high economic value and thick meat. Tilapia is easy to breed, uncontrolled spawning can occur so that growth is inhibited. The growth of male tilapia is faster than females at the same age. To increase the growth rate, sex reversal is carried out towards males through immersion in steroid hormones. Bioactive substances contained in sea urchins are steroid compounds. The purpose of this study is to determine the effect of the length of immersion in sea urchin gonad extract (Diadema setosum) on the formation of male phenotypes of tilapia larvae and tilapia survival. The method used is a completely randomized design with 4 treatments and each treatment with 3 replicates at a dose of 4 mg/L with soaking times of 0, 12, 18, and 24 hours. Data are analyzed using SPSS 16 software with analysis of variance (One-way ANOVA). The results show that different lengths of immersion in sea urchin steroid extract at a dose of 4 mg/L affect the formation of male individuals. A soaking time of 18 hours is quite effective in directing the sex of fish to males by 66%. Immersion time also affects the survival of tilapia larvae.
Stomata Diversity Profile of Several Coffee Species at the Indonesian Coffee and Cocoa Research Center Hapsari, Tasya; Munandar, Kukuh; Wibowo, Ari
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 2 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i2.331

Abstract

Stomata are a special part of plants, namely a number of leaf epidermal cells, both the upper and lower surface epidermis of the leaves. Stomata function as a place for CO2 to enter from the air in the process of photosynthesis, where respiration and transpiration take place so that stomata are an important tissue in plant metabolic processes. Observing stomata using a microscope is an effective and easy way to determine the type of stomata in angiosperm plants. This research was conducted on November 19 2022 with the aim of determining the type and diversity of stomata on several types of coffee plants at the Indonesian Coffee and Cocoa Research Center. The method used in this research is an exploratory survey method, while the replica method is used to look at leaf stomata, namely applying nail polish to the leaf surface and identifying the type of stomata using a qualitative descriptive method. The survey found that there were 9 types of coffee plants at the Indonesian Coffee and Cocoa Research Center. The results of the research show that the type of stomata on coffee plants based on the number and arrangement of neighboring cells is classified as a parasitic type. This can be seen from the presence of two neighboring cells surrounding the guard cell and the location of these cells parallel to the guard cell.
Effect of Sugar Type Variation on Nata de Coco Growth Attaqy, Fiella; Kurniasih, Surti; Munarti, Munarti
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 2 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i2.333

Abstract

The utilization of old coconut still needs to be improved. Old coconut water that is wasted can pollute the soil because of the acetic acid content produced by the fermentation process. Waste of old coconut water can be used as a food product in the form of nata, which has economic value and reduces environmental pollution. Sucrose is a carbon source for making nata de coco because it is an energy source for Acetobacter xylinum bacteria. Varied carbon sources can have different effects on nata de coco growth. The study results are used as learning media in class X high school. This study aimed to determine the effect of coconut sugar, corn sugar, and rock sugar on the quality of nata de coco, the type of sugar that is best for nata de coco growth, and the feasibility of e-booklet learning media. This research used an experimental and one-factor, complete, randomized design. Data analysis used was organoleptic testing juxtaposed with hedonic test, wet weight calculation, thickness, and learning media validation. The results showed that sugar cubes had the highest average value with a wet weight of 292 gr and a thickness of 1.4 cm. The lowest average value is corn sugar, with an average wet weight of 172 grams and a thickness of 0.9 cm. Organoleptic and hedonic testing of corn sugar has the highest value of 14.09. The validated e-booklet has a percentage score of 88.18% with very feasible criteria. Based on the study results, the varied types of carbon sources significantly affect the quality of nata de coco assessed in terms of appearance and organoleptic aspects.
Insect Species Diversity in Bangek River Area Lubuk Minturun Padang Laselvy, Khairunnisa; Zulyusri, Zulyusri
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.355

Abstract

The study employed the insect net method to sample insects from the Bangek River. Primary and secondary data were collected for analysis. The research was conducted at Bangek River, Lubuk Minturun District, Padang City, West Sumatra Province. Insect sampling involved a single event using an insect net within the administrative area of Lubuk Minturun. A total of 35 insects were captured from the Bangek River, comprising 5 species from the Lepidoptera order, 28 from the Odonata order, and 1 species each from the Hemiptera and Orthoptera orders.
Isolation and Identification of Fungi from Bactrocera dorsalis as Candidate Entomopathogenic Fungi Damayanti, Rizka Dwi; Emantis Rosa; Setiawan, Wawan Abdullah; Handayani, Tundjung Tripeni
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.359

Abstract

Cultivation of horticultural crops especially fruits, has long been practiced in Indonesia. Citrus plants are one of the main fruit commodities developed in Indonesia. Increased citrus production is inseparable from challenges, especially in controlling harmful pest attacks. Bactrocera fruit fly has attracted worldwide attention because of its ability to attack more than a hundred horticultural crops and cause crop failure. Various ways have been done to control Bactrocera, such as protecting the fruit with wrapping and even the use of synthetic insecticides that cause many losses. To overcome this, innovations are needed using biological control. Entomopathogenic fungi are potential candidates for bioinsecticides. This study aims to isolate and identify entomopathogenic fungi from Bactrocera dorsalis from citrus plants. The entomopathogenic fungus isolation method uses the moist chamber method. Then the fungal isolates will be identified macroscopically and microscopically. The isolation results show that five types of fungi are identified from the body of Bactrocera dorsalis. The five fungi can be used as candidates for entomopathogenic fungi.
The Effectiveness of Nigrospora sp. and Penicillium sp. As Entomopathogenic Fungi Against in Bactrocera sp. Oktariana, Putri; Rosa, Emantis; Setiawan, Wawan Abdullah; Nurcahyani, Nuning
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.361

Abstract

Pest attacks caused by fruit flies (Bactrocera sp.) can cause damage to fruit quality. Damage can become more severe due to secondary infection by bacteria or fungi which can cause fruit to rot. This research was conducted to determine the effectiveness and morphological changes caused by the fungus Nigrospora sp. and Penicillium sp. on the mortality of Bactrocera sp. This research was conducted using a Randomized Block Design (RBD) with two factors: the type of entomopathogenic fungi and the density of spores. Entomopathogenic fungi isolates were obtained from the isolation of entomopathogenic fungi from fruit flies (Bactrocera sp.) from previous studies. The spore density used for each fungus was 105, 106, and 107 spores/mL. Each treatment unit used 5 Bactrocera sp. adults with 3 repetitions. In the isolates of Nigrospora sp. they have resulted in mortality at a spore density of 105 spores/mL of 86%, a density of 106 spores/mL of 93%, and a density of 107 spores/mL of 100%. In the isolates of Penicillium sp. all treatments resulted in 100% mortality.
Helminthiasis and Factors Affecting Nutritional Status of Pregnant Women at Karang Anyar Health Center Jati Agung, South Lampung: Helminthiasis dan Faktor yang Mempengaruhi Status Gizi Ibu Hamil di Puskesmas Karang Anyar Jati Agung Lampung Selatan Hardianto, Devina; Suwandi, Jhons Fatriyadi; Zuraida, Reni; Setyaningrum, Endah
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.363

Abstract

Nutrition in pregnancy is needed for fetal growth and development. Insufficient nutrition has the risk of causing Chronic Energy Deficiency (CED). The CED rate among pregnant women in Indonesia is 17.3% and in Lampung Province it is 13.6%. Nutritional status is influenced by nutritional intake and helminthiasis infection. Apart from that, there are other factors such as social determinants of health, namely economics, education, employment and age. This research uses a cross-sectional research design. The research sample was 86 pregnant women. Data collection was carried out using a questionnaire, measurement of Upper Arm Circumference (MUAC), and examination of feces using the formol ether sedimentation method. Bivariate analysis used chi square, Fisher, and Mann Whitney. The results of the study showed that there was a significant relationship between helminthiasis (p = 0.009), education (p = 0.023), and per capita income (p < 0.001) with the nutritional status of pregnant women. Meanwhile, for other variables (knowledge about helminthiasis, preventive behavior, age, and occupation) there was no significant relationship (p > 0.05). Helminthiasis is related to the nutritional status of pregnant women, so active screening is needed for the incidence of helminthiasis in pregnant women in the working area of the Karang Anyar Community Health Center, Jati Agung District, South Lampung Regency.

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