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Jurnal Biogenerasi
Published by Universitas Cokroaminoto Palopo
ISSN : 25795163     EISSN : 25797085     DOI : -
Core Subject : Education,
Education Languange, Linguistic, Communication & Media Other
Biogenerasi focuses on the publication of the results of biology education,Biology learning model, Biology learning media, Biology Education Development, The article published on the internal and external academic community Universitas Cokroaminoto Palopo especially in Biology Education.
Arjuna Subject : Umum - Umum
Articles 939 Documents
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ANALISIS FINGERPRINT DNA GENOTIPE BELIMBING DENGAN GENUS Averrhoa MENGGUNAKAN PRIMER RANDOM AMPLIFIED POLYMORPHIC Hikmah, Nur; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/5d69v436

Abstract

Fingerprinting is a technique in DNA technology that can be used to see individual diversity and can also differentiate individuals from each other even though they are very closely related. This study aims to see the results of DNA fingerprinting of starfruit genotypes with the genus Averrhoa using Random Amplified Polymorphic DNA (RAPD) primers and to see the polymorphism value of each Random Amplified Polymorphic DNA (RAPD) primer. The research method used is Fingerprint DNA with Random Amplified Polymorphic DNA (RAPD) primers, namely OPA-2, OPA-3, OPA-5, OPA-7, OPD-11 and OPD-13. The results of the research show that the fingerprint results can be seen clearly. using electrophoresis. The best results were the primers OPA 2, OPA3 with 6 band patterns, followed by the second highest number of OPA5, OPD 11 with 5 band patterns, and the third highest number of OPA7, OPD 13 with 4 DNA fingerprint band patterns, the results of the kinship relationship between each Averrhoa have The polymorphic value shows that sweet starfruit (Averrhoa carambola L.) has a relationship of around 0.62% with starfruit. wuluh ( Averrhoa bilimbi L.). The Demak starfruit (Averrhoa carambola Cultivar Demak) has a relationship of around 0.71% with the Sembiring starfruit (Averrhoa carambola).  
ANALISIS KERAGAMANGENETIK DNA UBI JALAR (Ipomea batatas L.)DENGAN MARKA RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)   Wulan Dari, Tri; Idami, Zahratul; Manalu, Kartika
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/2dg8y347

Abstract

Sweet potato (Ipomea batatas L.) is a national food security supported by one of the potential plant resources. After rice, corn, and cassava, sweet potato (Ipomea batatas L.) contains the fourth highest carbohydrate. Sweet potato is a good source of thiamine (vitamin B1), riboflavin, vitamin A, vitamin B, and vitamin C. The purpose of this study was to determine the genetics of the analysis results presented in sweet potato (Ipomea batatas L.) using dendograms and DNA banding patterns in sweet potatoes based on RAPD markers. The research method used is an experimental method to collect data by conducting direct experiments, isolating DNA, and conducting PCR using the RAPD technique; Six RAPD primers were used: OPA 2, OPA 3, OPA5, OPA7, OPD 11, and OPD 13. The study resulted in genetic diversity seen from the DNA banding pattern that forms polymorphism. RAPD produces bands measuring 100-900 bp. The genetic diversity of 4 types of Ipomea batatas L. plants analyzed using the NTSYS pc-2.02i application on the kinship relationship displayed with a dendogram shows the same diversity, namely orange sweet potatoes with yellow sweet potatoes, which have the highest level of similarity because they join at a coefficient value of around 0.91. While in purple sweet potatoes joining the group (yellow sweet potatoes and orange sweet potatoes) at a coefficient value of 0.86 indicates that purple sweet potatoes are slightly more different but still in the group. Then in white sweet potatoes have a connection coefficient value of around 0.81 which indicates that this sample is the most different compared to the others)
IDENTIFIKASI JENIS-JENIS KEPITING (BRACHYURA) YANG DIPERJUAL-BELIKAN DI PASAR KECAMATAN PADANG BARAT, KOTA PADANG Rahmadana; Satria, Rijal
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/w1fps817

Abstract

Crab trade in traditional markets is an important part of the fisheries distribution chain that reflects the utilization of marine resources by coastal communities. This study aims to identify the types of crabs (Brachyura) traded in traditional markets in Padang Barat District, Padang City, and to provide basic information for sustainable fisheries resource management. The research was conducted in February 2026 through direct observation at three traditional markets, namely Purus Fish Market, Raden Saleh Market, and Padang City Central Market. Data were collected through specimen documentation, species recording, and brief interviews with traders. The results of the study show that there are four species of crabs traded, all of which belong to the Portunidae family, namely Scylla serrata, Charybdis feriata, Portunus sanguinolentus, and Portunus pelagicus. The dominance of the Portunidae family indicates that swimming crabs are the main commodity in the marine product trade in the coastal area of West Sumatra. This study provides basic data on crab utilization in traditional markets and can support fisheries management, conservation of biological resources, and coastal economic development based on biodiversity.
VARIASI MORFOLOGI SISIK PADA IKAN FAMILI CYNOGLOSSIDAE: SEBUAH TINJAUAN LITERATUR Rahmadhani, Suchy; Razak, Abdul
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/pw76kf76

Abstract

The tonguefish (family Cynoglossidae) is characterized by its flattened body and is widely distributed across the Indo-Pacific, India, Pakistan, and the Red Sea. This study aims to analyze the variation of scale types in the family Cynoglossidae based on a literature review. The literature search was conducted using electronic databases, namely Google Scholar, PubMed, and ScienceDirect. The keywords used in the search were "Cynoglossidae", "scale morphology", "tonguefish", and "scales". A total of 9 articles met the inclusion criteria and were extracted for data. Fifteen species of tonguefish were successfully identified in terms of their scale types. Three patterns of scale variation were found: ctenoid scales on both sides, ctenoid scales on the eyed side and cycloid scales on the blind side, and cycloid scales on both sides. The conclusion of this review is that the scale types in the family Cynoglossidae vary among species and show consistent patterns, thus they can serve as supporting characters in taxonomic identification and classification.
EXPLORATION AND IDENTIFICATION OF MICROBES AND FUNGI FROM SOIL SAMPLES OF GUNGGUNG VILLAGE, BATUAN DISTRICT, SUMENEP REGENCY AS BIOLOGICAL CONTROL AGENTS AT THE EAST JAVA FOOD CROPS AND HORTICULTURE PLANT PROTECTION LABORATORY   Anindita Darma, Diva; Misnati Nur Indahsari, Wiwin
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/b4ev2f20

Abstract

Soil microorganisms play an essential role in maintaining soil fertility and suppressing plant pathogens, making them potential biological control agents in sustainable agriculture. This study aimed to explore and identify microbes and fungi from soil samples collected from yellow Kepok banana (Musa spp.) plantations in Gunggung Village, Batuan District, Sumenep Regency, as potential biological agents. The novelty of this research lies in the exploration of untreated Fusarium-infected banana plantation soil to obtain indigenous microbial isolates with biocontrol potential. A descriptive exploratory research design was employed. Bacterial isolation was conducted using the serial dilution method on Potato Dextrose Agar (PDA), followed by purification, macroscopic and microscopic characterization, 5% KOH test, soft rot test, and hypersensitivity test. Fungal isolation was performed using the baiting method with coconut, rice, and mealworms as substrates, followed by morphological identification. The results revealed the presence of Bacillus sp. as the dominant bacterial isolate, characterized by circular colonies, yellowish-white color, Gram-positive reaction, and rod-shaped cells. Soft rot and hypersensitivity assays indicated varying pathogenic responses among isolates. Fungal identification showed the presence of Aspergillus sp. (brownish-green, powdery colonies) and Penicillium sp. (yellowish-green, granular colonies). These findings indicate that soil from the study site contains microbial populations with potential applications as biological control agents. The study highlights the importance of exploring indigenous soil microorganisms to support environmentally friendly plant disease management and sustainable agricultural practices.
GENOME EDITING BERBASIS CRISPR-CAS9 UNTUK MENINGKATKAN TOLERANSI STRES ABIOTIK PADA TANAMAN PADI (ORYZA SATIVA): A SYSTEMATIC LITERATURE REVIEW Putriwan, Maya; Sumarni; Rachmawaty
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/ydmgry18

Abstract

Abiotic stresses such as drought, salinity, extreme temperatures, and oxidative stress are major factors limiting the growth and productivity of rice plants. The development of CRISPR-Cas9-based genome editing technology provides a new, more precise approach to modifying genes that play a role in plants' adaptive responses to environmental stresses. This study aims to synthesize the development of genome editing research in rice plants, focusing on the identification of target genes, genome editing strategies, and their impact on increasing abiotic stress tolerance. The method used is a systematic literature review of research articles published in international scientific journals. A total of 31 articles were analyzed in the literature review process, and 22 articles that explicitly reported target genes and genome editing strategies were used in the main synthesis. The results of the analysis showed that most studies targeted regulatory genes that play a role in regulating stress responses, especially groups of transcription factors such as NAC, DREB, bZIP, MYB, and WRKY. In addition, several studies also targeted genes related to ion homeostasis, hormonal signaling pathways, secondary metabolism, and antioxidant systems. The most dominant genome editing strategy was gene knock-out through CRISPR-Cas9-based mutations, while several recent studies have begun to apply multiplex genome editing to target more than one gene simultaneously. Genome-edited rice plants generally show increased tolerance to various abiotic stresses through improved ion homeostasis, increased water use efficiency, cell membrane stability, and strengthened antioxidant systems. These findings indicate that CRISPR-Cas9 is a precision genome engineering approach with the potential to support the development of rice varieties that are more adaptive to environmental changes.  
NARRATIVE REVIEW: POTENSI EKSTRAK BAWANG PUTIH (Allium sativum) DALAM MENINGKATKAN PERTUMBUHAN LACTOBACILLUS DAN MENGHAMBAT BAKTERI PENYEBAB DIARE Amanda, Fadillah; Azzahra Putri Hartono, Olyvia; Atifah, Yusni
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/khm94k57

Abstract

Diarrhea is a global health challenge exacerbated by increasing antibiotic resistance. This narrative review aims to analyze the potential of garlic extract (Allium sativum) as a natural synbiotic agent in supporting Lactobacillus growth while suppressing enteric pathogens. The method used was a narrative review with systematic literature searches through the Google Scholar and PubMed databases. The synthesis results show that garlic contains allicin and fructooligosaccharide (FOS) compounds at 3.34% w/w, which are effective in inhibiting pathogenic bacteria such as Escherichia coli and Salmonella spp., and are able to stimulate the growth of Lactobacillus acidophilus optimally at a concentration of 4%. In conclusion, Allium sativum has dual potential as an antibacterial agent and prebiotic, whose effectiveness is influenced by concentration and extraction method. Further research is needed to standardize the dosage for clinical application.
EVALUASI PENERAPAN PRINSIP BIOETIKA DALAM KESEJAHTERAAN HEWAN (ANIMAL WELFARE) BERDASARKAN FIVE FREEDOMS PADA SATWA DI PUSAT PENYELAMATAN SATWA TAMAN WISATA ALAM SIBOLANGIT Nabila Syahfitri; Nazwa Syafitri; Muhammad Yusuf; Khairiza Lubis; Widya Arwita
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/8hq46e10

Abstract

Penelitian ini bertujuan untuk mengevaluasi penerapan prinsip bioetika dalam kesejahteraan hewan (animal welfare) berdasarkan konsep Five Freedoms pada satwa di Pusat Penyelamatan Satwa (PPS) Taman Wisata Alam Sibolangit. Metode yang digunakan adalah deskriptif dengan pendekatan kuantitatif melalui observasi langsung dan dokumentasi. Penilaian dilakukan terhadap beberapa indikator, yaitu ketersediaan pakan, kebersihan kandang, kondisi kesehatan satwa, ukuran kandang, dan pengamatan perilaku satwa. Hasil penelitian menunjukkan bahwa pengelolaan satwa di PPS TWA Sibolangit telah memenuhi sebagian besar prinsip Five Freedoms, meliputi bebas dari lapar dan haus, bebas dari ketidaknyamanan, bebas dari penyakit, bebas mengekspresikan perilaku alami, serta bebas dari stres. Nilai kesejahteraan satwa diperoleh sebesar 92% yang termasuk dalam kategori sangat baik. Secara bioetika, pengelolaan satwa telah mencerminkan prinsip beneficence dan non-maleficence. Namun demikian, masih diperlukan peningkatan pada aspek ukuran kandang, pengayaan lingkungan, dan frekuensi pemberian pakan agar kesejahteraan satwa dapat lebih optimal.
HUBUNGAN SIKAP IBU DENGAN KEJADIAN UNDERWEIGHT PADA BALITA DI WILAYAH KERJA PUSKESMAS COT SEUMEURENG   Muriza Rahmatul Husna; Sri Wahyuni Muhsin; Ravi Masitah; Cukri Rahma
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/x4d0es65

Abstract

Good nutritional status in toddlers is essential for child health and development. Nationally, the prevalence of underweight among children under five in Indonesia is 15.9%, consisting of 3.0% severely underweight and 12.9% underweight, while 84.1% of toddlers have normal nutritional status. At the provincial level, Aceh shows a higher prevalence of underweight at 22.6%, including 4.9% severely underweight, 17.7% underweight, and 77.4% with normal nutritional status. At the district level, data from the Aceh Barat Health Office in 2024 recorded 530 cases of underweight, with the highest distribution in the working areas of Cot Seumeureung Health Center (85 cases), Kuala Bhee Health Center (84 cases), and Johan Pahlawan Health Center (83 cases).This condition highlights the importance of the mother’s role, particularly maternal attitudes toward fulfilling toddlers’ nutritional needs. This study aimed to analyze the relationship between maternal attitudes and the incidence of underweight among toddlers in the working area of Cot Seumeureung Health Center. A quantitative cross-sectional design was used, involving 63 mothers of children aged 0–59 months from three villages through total sampling. Data were collected from November to December 2025 using questionnaires, interviews, and anthropometric measurements based on WHO weight-for-age (WFA) z-score standards, and analyzed using the chi-square test (α = 0.05). The results showed that most mothers had positive attitudes toward toddler nutrition, and there was a significant relationship between maternal attitudes and the incidence of underweight (p = 0.040). In conclusion, maternal attitudes play an important role in determining toddlers’ nutritional status; therefore, underweight prevention efforts should focus on fostering positive attitudes, appropriate feeding practices, and strengthening continuous nutrition education and assistance through integrated health posts (posyandu) and health workers.
KAJIAN LITERATUR MIKROSTRUKTUR SISIK IKAN DALAM STUDI TAKSONOMI IKAN Raesya Cholida; Abdul Razak
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/str2bq15

Abstract

Taksonomi ikan umumnya menggunakan pendekatan morfometri dan meristik dalam proses identifikasi spesies, namun metode tersebut memiliki keterbatasan pada spesies yang berkerabat dekat dan memiliki kemiripan morfologi eksternal. Kajian ini bertujuan menelaah peran mikrostruktur sisik sebagai karakter taksonomi pendukung dalam studi identifikasi ikan. Penelitian dilakukan melalui metode systematic literature review terhadap berbagai publikasi ilmiah yang membahas analisis mikrostruktur sisik menggunakan mikroskop cahaya dan scanning electron microscope (SEM). Hasil kajian menunjukkan bahwa komponen mikrostruktur seperti fokus, circuli, radii, lepidont, tubercle, ctenii, serta struktur canal lateral memiliki variasi yang konsisten antarspesies maupun antar lokasi sisik pada tubuh ikan. Perbedaan karakter tersebut terbukti mampu membedakan spesies yang secara morfologi makroskopis sulit dipisahkan, serta sejalan dengan data kekerabatan molekuler. Selain pada ikan modern, mikrostruktur sisik fosil juga dapat digunakan untuk interpretasi paleoekologi dan estimasi umur. Dengan demikian, analisis mikrostruktur sisik berpotensi menjadi karakter taksonomi pelengkap yang penting dalam memperkuat sistem identifikasi dan klasifikasi ikan.

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