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Contact Name
Prof. Dr. Imam Mustofa, drh., M.Kes
Contact Email
imam.mustofa@fkh.unair.ac.id
Phone
+62315992377
Journal Mail Official
ovozoa@journal.unair.ac.id
Editorial Address
Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlaangga, Kampus C Mulyorejo – 60115, Surabaya 3015
Location
Kota surabaya,
Jawa timur
INDONESIA
Ovozoa: Journal of Animal Reproduction
Published by Universitas Airlangga
Core Subject : Health, Agriculture,
Ovozoa Journal of Animal Reproduction (Ovozoa J Anim Reprod) published original articles, review articles, and case reports in Indonesian or English, in the scope of veterinary reproduction and animal reproductive biotechnology from researchers, lecturers, students, and practitioners around Indonesia and worldwide. The main objective of the Ovozoa J Anim Reprod is to disseminate the results of scientific researches in the field of animal reproduction. Ovozoa J Anim Reprod invites manuscripts in the areas:  Development of reproductive diagnostic techniques  Development of reproductive technologies  Infectious and non-infectious reproductive diseases  Comparative pathology of reproductive diseases  Reproductive endocrinology  Infertility  Environmental and stress effects on reproduction  Better understanding of the in vivo fertilization process  Better understanding of embryonic development  Artificial insemination and embryo transfer  In vitro fertilisation and embryo culture  Sexual selection of sperm and embryo  Semen and embryo preservation  Reproductive cloning  Stem cell in reproduction  Development of assisted reproductive technologies  Molecular biology, genetics, proteomics, metabolomics application to reproductive biology
Articles 161 Documents
Anti-androgenic and antifertility activity of Azadirachta indica seed oil in male albino rats Onwuka, Ositadinma Sunday; Oguejiofor, Chike Fidelis; Njoga, Ugochinyere Juliet; Ochiogu, Izuchukwu Shedrack; Eke, Ifeanyi Gabriel
Ovozoa: Journal of Animal Reproduction Vol. 15 No. 1 (2026): Ovozoa: Journal of Animal Reproduction (IN PRESS)
Publisher : Universitas Airlangga

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Abstract

Two experimental phases (I and II) were conducted to investigate the reproductive effects of Azadirachta indica methanol seed extract (AIMSE) in adult male albino rats. In Phase I, male rats were randomly assigned (fourteen per group) to four groups: a control group (A) and three groups treated orally with graded doses of AIMSE at 50 mg/kg (B), 100 mg/kg (C) and 200 mg/kg (D) for 28 days. Animals were evaluated on day 0 (pre-treatment baseline), day 14 and day 28 for haematology, serum biochemistry and hormone concentrations, relative organ weights, sperm analysis and histopathology of the testis and epididymis. In Phase II, untreated fertile female rats were bred (four per group) with four male rats from each group (A-D) to assess post-treatment male fertility. Mated females were evaluated for several reproductive indices. In Phase I, AIMSE-treated males showed reduced serum testosterone concentrations, decreased vitality and motility of epididymal spermatozoa, and histopathological evidence of degeneration in the testis and epididymis. In Phase II, treatment with AIMSE for 28 days did not prevent mating by males and pregnancy in bred females; however, it induced significant antifertility effects, including decreased gestation index, reduced litter size and litter weight, increased pre-implantation loss, and decreased post-implantation embryo survival. These findings provide evidence of a dose-dependent in vivo anti-reproductive effects of AIMSE in male rats and, more importantly, a lowered fertility in females mated with treated males.