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Prof. Dr. Imam Mustofa, drh., M.Kes
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Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlaangga, Kampus C Mulyorejo – 60115, Surabaya 3015
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Ovozoa: Journal of Animal Reproduction
Published by Universitas Airlangga
ISSN : 23026464     EISSN : 2722967X     DOI : http://dx.doi.org/10.20473/ovz.v9i1.2020.1-6
Core Subject : Health, Agriculture,
Agriculture, Biological Sciences & Forestry Veterinary
Ovozoa Journal of Animal Reproduction (Ovozoa J Anim Reprod) published original articles, review articles, and case reports in Indonesian or English, in the scope of veterinary reproduction and animal reproductive biotechnology from researchers, lecturers, students, and practitioners around Indonesia and worldwide. The main objective of the Ovozoa J Anim Reprod is to disseminate the results of scientific researches in the field of animal reproduction. Ovozoa J Anim Reprod invites manuscripts in the areas:  Development of reproductive diagnostic techniques  Development of reproductive technologies  Infectious and non-infectious reproductive diseases  Comparative pathology of reproductive diseases  Reproductive endocrinology  Infertility  Environmental and stress effects on reproduction  Better understanding of the in vivo fertilization process  Better understanding of embryonic development  Artificial insemination and embryo transfer  In vitro fertilisation and embryo culture  Sexual selection of sperm and embryo  Semen and embryo preservation  Reproductive cloning  Stem cell in reproduction  Development of assisted reproductive technologies  Molecular biology, genetics, proteomics, metabolomics application to reproductive biology
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 161 Documents
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White guava (Psidium guajava L.) fruit juice ameliorated the number of spermatogenic cells in rats (Rattus Novergicus) exposed to lead acetate Muhammad Faizal Adiutomo; Suzanita Utama; Herry Agoes Hermadi; Wurlina Wurlina; Hardany Primarizky; Rochmah Kurnijasanti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.39-46

Abstract

This study aims to determine the effect of oral administration of white guava fruit juice (Psidium guajava L.) on the spermatogenic cells of rats (Rattus norvegicus) exposed orally to lead acetate. Twenty-five male rats (Rattus norvegicus) of the Wistar strain were randomly divided into five groups. Placebo group rats (P0) were given 0.5 mL distilled water twice every day orally at four-hour intervals. Groups T0, T1, T2, and T3 were given lead acetate 50 mg/kg bw orally, then respectively given distilled water, 100, 50 and 25% (v/v) white guava (Psidium guajava L.) fruit juice orally four hours later for 14 days. On day-15, the rats were sacrificed and their testicles were taken for histological preparations. Data were analyzed using Analysis of Variance followed by Duncan's Multiple Range Test. The results showed that the number of spermatogonia, spermatocytes and spermatids cells of rats in T0 group was lower (p <0.05) than in P0 group. Higher spermatogonia, spermatocytes and spermatids cells (p <0.05) were found in the T1 group compared to the T0 group. However, the number of spermatogonia and spermatocytes in the T1 group was still lower than in the P0 group. The number of spermatids in the T3 group was almost the same (p >0.05) as in the P0 group. This study found that white guava pure juice without dilution was the best for maintaining the number of spermatids, but was unable to restore the number of spermatogonia and spermatocyte cells in rats exposed to lead acetate.
Addition of dextrose and caffeine to the intravenous treatment of milk fever in Holstein Friesian cattle Ady Kurnianto; Novianto Meska Pratama
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.172-180

Abstract

A farmer reported a cow unable to stand 48 hours postpartum. The cow was a 2.5 years old dairy cow with a body weight of around 350 kg. The cow was fed elephant grass, standard concentrate (16–18%) crude protein, and drinking water ad libitum without mineral supplementation during the dry season. The cow's position and posture when lying down, ear temperature, and eye pupils were included in the physical examination. Availability of food and drink was examined to predict appetite and thirst. The results of the history and physical examination showed that the cow was unable to stand 48 hours after calving, had no appetite and eye reflexes, had cold ears, and trembling hind legs. The cow was laid on its right side, with its head turned to flank. Based on the anamnesis and physical examination, the cow was diagnosed as suffering from stage 2 puerperal hypocalcemia. The cow was infused via the jugular vein with a solution containing 270 mg calcium borogluconate, 70 mg magnesium borogluconate, 300 mg dextrose, 5 mg phosphorus element, 2 mg potassium chloride, and 5 mg caffeine per 1 mL of solution. Forty-five minutes after the infusion, the cow was able to stand, although it still looked weak. It could be concluded that the addition of dextrose and caffeine to the standard treatment of milk fever was beneficial for recovery.
The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat Annisa Nur Fitria Rahma; Sherina Lashita Candrakirana; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Budi Utomo; Tatik Hernawati; Anwar Ma'ruf; Nove Hidajati; Kadek Rachmawati; Eka Pramyrtha Hestianah; Hani Plumeriastuti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.30-38

Abstract

This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.
Reproductive disorders of cows in several villages of Kedamean district, Gresik regency, East Java, Indonesia in 2023 Utomo, Budi; Rimayanti, Rimayanti; Restiadi, Tjuk Imam; Amrullah, Muhammad Fajar
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.18-29

Abstract

Reproductive disorders are the main cause of economic losses in beef herds. This study aimed to determine the type of reproductive disorder in beef cows in Kedamean district, Gresik regency, related to feeding, age, and parity. The method used for this study was a survey. Primary data was obtained from field surveys by interviewing farmers, animal health officials, inseminators, and direct inspection of cows and pens. Secondary data was obtained from the Gresik Animal Husbandry and Animal Health Service. The data obtained was then compiled and performed descriptively. The results showed that the number of productive cows in this study area was 935, of which 340 (36.36%) had reproductive disorders. The highest type of reproductive disorder was ovarian hypofunction (OHF) (14.55%, 136/935) and the smallest was cystic ovaries (0.11%, 1/935). Cows aged two and three years (heifers and first parity) have a percentage of reproductive disorders of more than 60% compared to cows of other ages, most of them (13.82% and 12.65%) were OHF. The cows fed straw have a greater percentage of reproductive disorders than cows fed field grass. Cases of OHF engaged the first position followed by cases of silent estrus and persistent corpus luteum. It could be concluded that reproductive disorders in the form of OHF, persistent corpus luteum, and silent estrus in several villages in Kedamean district, Gresik regency, East Java, Indonesia in 2023 mostly occured at the age of two to three years on heifers or first parity, fed straw.
Managing retained placenta in first-parity doe and administering vitamin A, D, and E as supportive treatment Dhea Salsabila; Viski Fitri Hendrawan
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 1 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i1.2024.58-66

Abstract

This study aims to report the treatment of retained placenta in a doe by administration of intrauterine antibiotics, accompanied by intramuscular injection of antibiotics, anti-inflammatory, and injection of vitamins A, D, and E as supportive treatment. The Saanen doe was brown, approximately two years old with a BCS of 3/5 and kidding for the first time on January 11, 2023. The following day, it was reported that the doe's placenta had not been expelled until 24 hours after kidding. Physically the doe was weak, unable to stand, and reddish-brown discharge was seen came out of the vulva. Based on these conditions the doe was diagnosed as having retained placenta with a fausta prognosis. The doe was treated with a bolus of antibiotics contained 250 mg sulphadiazine and 50 mg trimethoprim which was diluted with 5 mL of 0.9% NaCl for uterine lavage. Systemic treatment consisted of intramuscular injection of ceftiofur at 1.1 mg/kg bw, flunixin meglumine at 1.1 mg/kg bw, as well as a combination of 300,000 IU of vitamin A palmitate, 100,000 IU of vitamin D3, and 50 mg of vitamin E acetate as supportive treatment. The treatment was successful, the doe was able to stand and eat when examined the next day after treatment. The doe returned to estrus 42 days after treatment. It could be concluded that treatment of retained placenta in a doe with intrauterine broad-spectrum antibiotics, beta-lactamase antibiotics and intramuscular anti-inflammatory, with vitamins A, D and E as supportive therapy was effective and the doe returned to estrus 42 days after treatment.
Coconut water combined with purebred chicken egg yolk as an alternative semen extender for Madura Pote bucks semen preservation Tatik Hernawati; Susilowati, Suherni; Tri Wahyu Suprayogi; Widya Paramita Lokapirnasari; Zulfi Nur Amrina Rosyada
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.96-104

Abstract

One of the many efforts to increase the quality of livestock genetics is through artificial insemination (AI). Other than increasing it, AI can be conducted to preserve semen. A successful AI is determined by seminal quality, therefore, a method to preserve semen for a longer storage time is needed. The method used is adding an extender that fulfills prerequisites for a semen extender such as coconut water combined with egg yolk citrate extender. Coconut water is rich in carbohydrates, protein, vitamins, and antioxidants while egg yolk contains lecithin. This study aims to find out the Pote buck spermatozoa quality stored in coconut water and egg yolk extender. This study uses three groups of treatments (T0: 0.1 ml semen + 0.9 ml egg yolk citrate, T1: 0.1 ml semen + 0.9 ml coconut water, and T2: 0.1 ml semen + egg yolk citrate (20%) + coconut water). All three of these are stored at 5oC and evaluated every day until day 5 of their motility, viability, intact plasma membrane, abnormality, and MDA level. Data analysis used is ANOVA and a further test called BNT is conducted if a significant difference is determined. No significant difference was found between T0 and T1 (p>0.05). The highest progressive motility, viability, and intact plasma membrane (%) among the three groups of treatments happened to be from T2. Meanwhile, a low percentage of spermatozoa abnormality and MDA level were also found in T2 with its extender being coconut water combined with egg yolk citrate. To conclude, the best extender for storing Pote buck semen is stored at 5oC is coconut water combined with egg yolk citrate extender.
Effect of PG-600 dose in cows with persistent corpus luteum treated with PGF2α and hCG Sofia Brenieta Brilianti; Sri Mulyati; Wurlina Wurlina; Budi Utomo; Oky Setyo Widodo
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.90-95

Abstract

The purpose of this study was to determine the effect of administering a dose of gonadotropin (PG-600) in cows with persistent corpus luteum (PCL) which were treated with prostaglandin F2 α (PGF2α) and human chorionic gonadotropin (hCG). This study used 20, three-year-old cows diagnosed with PCL, had recently given birth, and were not pregnant. Cows were randomly allocated into groups T0, T1, T2 and T3.  Cows in all groups were injected with PGF2α (25 mg/cow). Simultaneously, cows in groups T1, T2 and T3 were also injected with PG-600 at doses of 100, 150 and 200 IU/cow respectively. PGF2α and PG-600 were injected when PCL was identified. After that, cows showing signs of estrus were injected with hCG (100 IU/cow) and artificially inseminated 12-18 hours after the appearance of signs of estrus. AI was conducted using frozen thawed semen. Sixty days following AI, a pregnancy examination was conducted using USG. Estrus was detected in all treated cows, while the pregnancy rates varied. Compared with other groups, the groups given 100 and 150 IU PG-600 produced higher pregnancy rates. It could be concluded that the combination of 25 mg PGF2α, 150 IU PG-600, and 100 IU hCG was the most effective treatment to produce high pregnancy rates in cows with persistent corpus luteum.
Mulberry (Morus alba L.) leaf extract enhanced spermatozoa motility, viability, and plasma membrane integrity of rats (Rattus norvegicus) exposed to e-cigarette smoke Ramadhani, Fifi Fauziah; Yulianna Puspitasari; Hermin Ratnani; Budi Utomo; Rochmah Kurnijasanti; Kadek Rachmawati
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.120-128

Abstract

This study investigates the impact of mulberry leaf extract on the viability, plasma membrane integrity, and motility of spermatozoa from male white rats (Rattus norvegicus) exposed to e-cigarette smoke. A total of twenty-five male rats were divided into five groups: negative control (NC), positive control (PC), T1, T2, and T3. All groups, except for the NC group, were exposed to e-cigarette smoke. Rats in the T1, T2, and T3 groups received mulberry leaf extract in doses of 100, 200, and 400 mg/kg bw, respectively, while the NC and PC groups were given a placebo of 1% Na-CMC. Both the mulberry leaf extract and the placebo were administered daily, beginning three days prior to the start of e-cigarette smoke exposure, which lasted for 28 days. Results showed that spermatozoa motility, plasma membrane integrity, and viability in the experimental groups were significantly lower than those in the NC group (p <0.05). Conversely, rats in the T1, T2, and T3 groups that received mulberry leaf extract demonstrated significantly greater spermatozoa viability, plasma membrane integrity, and motility compared to the PC group (p <0.05). The T3 group exhibited the most pronounced improvements, with significantly enhanced spermatozoa viability, membrane integrity, and motility (p <0.05) relative to the PC group. These results indicate that mulberry (Morus alba L.) leaf extract enhanced spermatozoa viability, plasma membrane integrity, and motility in white rats (Rattus norvegicus) subjected to e-cigarette smoke.
Sex difference, growth pattern, and cephalic index of Turi ducks at different ages based on head morphometry Bangun Nur Wijaya; Yeni Dhamayanti; Sri Mulyati; Soeharsono Soeharsono; Sunaryo Hadi Warsito; Sri Hidanah
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.105-114

Abstract

This study aims to determine the morphometric differences in the head of male and female Turi ducks. Ten male and ten female day-old ducks (DOD) were randomly selected by a lottery system from the population, raised, and had their head morphology measured using a digital caliper with an accuracy of 0.01 mm on the first day, and at the age of 2, 4, 6, and 8weeks. The result showed that male Turi duck had wider (p <0.05) head at the ages of 1 day, 2 and 6 weeks, head length and head height at the ages of 1 day, 2, 4, 6, and 8 weeks, rostrum width at the ages of 1 day, 4, 6, and 8 weeks, and rostrum length at the age of 2, 4, 6 and 8 weeks. There were no significant differences (p >0.05) in the head width at the ages of 4 and 8 weeks, rostrum width at 2 weeks, and rostrum length at 1day old male and female Turi ducks. Morphometric growth pattern showed that older Turi ducks (at a range of 1 day to 8 weeks) had larger (p <0.05) head morphometry. However, the cephalic index in male and female Turi ducks was not significantly different (p > 0.05) at all ages. It could be concluded that in general head morphometry was useful for determining male and female Turi ducks at the ages of 1 day, 2, 4, 6, and 8 weeks
Risk factors of pyometra in cat at several clinics in Kediri, East Java, Indonesia Rosyta, Pegy; Rimayanti Rimayanti; Budi Utomo; Jola Rahmahani; Yulianna Puspitasari; Wiwik Misaco Yuniarti
Ovozoa: Journal of Animal Reproduction Vol. 13 No. 2 (2024): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v13i2.2024.115-119

Abstract

Pyometra is a reproductive disease that most often affects cats which is caused by bacterial infection of the uterus. This study aims to determine the risk factors that could influence the occurrence of pyometra in cats. The study was conducted at the Four Leg Pet Care, Asa Animal Clinic, and K-5 Animal Care in Kediri regency in December 2021 to February 2022. This retrospective cohort study took secondary data for the period 2020 to 2021 as research material and found 35 cats diagnosed with pyometra. The odds ratio was calculated to analyze the associations to some factors including age, body weight, use of hormonal contraception, frequency of administration of hormonal contraception, hormonal contraceptive drugs used, dose of hormonal contraception given.  Analysis of the relationship between risk factors for pyometra showed that the factors age, use of hormonal contraception, and frequency of giving hormonal contraceptives each have p values ​​of 2.17, 2.13, and 2.13, respectively. It could be concluded that age, use of hormonal contraception, and frequency of administration of hormonal contraception influenced or had an association with the risk of pyometra in cats.

Page 13 of 17 | Total Record : 161

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