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The Potentials of Robusta Coffee Seed Extract as Antioxidant on Kidney Histopathology in Mice Exposed to Monosodium Glutamate: Potensi Pemberian Ekstrak Biji Kopi Robusta sebagai Antioksidan Terhadap Gambaran Histopatologi Ginjal Mencit yang Dipapar Monosodium Glutamat Wisudawati, Feby Dwi; Hidajati, Nove; Fikri, Faisal; Santoso, Kuncoro Puguh; Saputro, Amung Logam; Purnama, Muhammad Thohawi Elziyad
Veterinary Biomedical and Clinical Journal Vol. 5 No. 1 (2023)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2023.005.01.3

Abstract

The purpose of this study was to determine the potential of administration robusta coffee extract as an antioxidant on the renal histopathology in mice exposed to monosodium glutamate (MSG) orally. This study used 20 adult male rats with Balb C strain divided into 5 experimental groups, namely K + (MSG 0.12 mg / head and Vitamin C 6 mg/mice), K- (MSG 0.12 mg/mice and Sodium carboxymethyl cellulose (CMC Na) 0,1 ml/mice), P1 (MSG 0.12 mg/mice and Robusta coffee extract 0.1 mg/mice), P2 (MSG 0.12 mg/mice and Robusta coffee extract 0.2 mg/mice), and P3 (MSG 0.12 mg/mice and 0.4 mg Robusta coffee extract mg/mice). The experimental group was treated every day at intervals between MSG and Vitamin C, CMC Na, and Robusta coffee extract for 60 min, the treatment was carried out for 42 days. Kidney histopathology scores were analyzed using the Saphiro-Wilk and Levene test to evaluate homogeneity, followed by the Kruskal Wallis test. The results of the Saphiro Wilk and Levene statistical analysis obtained p<0.05, thus the data continued with the Kruskal Wallis test scores of kidney cell damage obtained 0.043 which showed that the data were significant. The mean score of kidney cell damage in the treatment groups was K+ 1.60; K- 1.63; P1 1.70; P2 1.63; and P3 1.73. The conclusion is that Robusta coffee bean extract did not show a decrease in kidney cell damage that was different between treatment groups. Further research is needed to find out about the effect of different dosages of Robusta Coffee Extract to different organs
Necrosis Description of Mice Liver Induced with Monosodium Glutamate and Methanol Robusta Coffee Bean Extract (Coffea canephora) Al-Zuhroh, Tisya Yumn; Santoso, Kuncoro Puguh; Yunita, Maya Nurwartanti; Hidajati, Nove; Praja, Ratih Novita
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.213-220

Abstract

This study aimed to analyze the effect of methanol robusta coffee bean extract (Coffea canephora) on liver histopathological description in mice induced with monosodium glutamate (MSG). This study was used 20 male mice for 5 treatment groups: K + (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) per orally for 42 days. Liver histological scores were analyzed using Kruskal Wallis test. The results of Kruskal Wallis test scored p=0,391 which indicates that the data is not significant (p>0.05). In the control and treatment groups there were no significant. In conclusion, the giving methanol robusta coffee bean extract did not show a decrease in hepatosite damage that was different from the negative control treatment group.
Essential Oil of Patchouli Inhalation Exhibits An Anxiolytic-Like Effect in Mice: Elevated Plus-Maze Test Johansyah, Fadilla Anita Kinksky; Hestianah, Eka Pramyrtha; Rimayanti, Rimayanti; Hidajati, Nove; Santoso, Kuncoro Puguh; Sudjarwo, Sri Agus; Hidayatik, Nanik
Jurnal Medik Veteriner Vol. 6 No. 3 (2023): Special Issue: International Conference on Veterinary Medicine and Health Scien
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss3.2023.31-36

Abstract

Anxiety disorder is one of the important public health problems. This study aimed to find out whether patchouli (Pogostemon cablin (Blanco) Benth.) essential oil (PEO) has an anxiolytic-like effect in mice. A total of 20 mice were divided into five treatment groups i.e. (C1) was treated with 1 mg/kg of saline intraperitoneally; (C2) was treated with 1 mg/kg of diazepam intraperitoneally; (T1) 1% PEO inhalation, (T2) 2.5% PEO inhalation, and (T3) 5% PEO inhalation. All experimental animals were put in the Elevated Plus-Maze (EPM), observed for five minutes, and investigated the following parameters i.e. the total number of squares that are transverse in the open arms (STO), the number of visits to the open arms (VOA), the number of visits to the closed arms (VCA), the time spent in the open arms (TOA), and the time spent in the closed arms (TCA). In results, T1 and T2 groups had a minor impact on the VOA. T1 group also had little effect on TOA. Closed-arm activities (VCA and TCA) were unaffected by any of the treatments. The conclusion was revealed that T1 or less of PEO inhalation can potentially provide anxiolytic-like effects in mice.
DESCRIPTION OF HEMATOLOGICAL VALUE IN LEOPARD GECKO (Eublepharis macularius) Wiranata, Try Ardhi; Widjiati, Widjiati; Wahyuni, Retno Sri; Hidajati, Nove; Poetranto, Emmanuel Djoko; Yuliani, M. Gandul Atik
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19990

Abstract

The aim of this research was to explore the hematological value of leopard gecko (Eublepharis macularius). Total of 20 samples were examined, its consists of 10 males and 10 females, blood samples were taken from vena ventral abdominal and deposited into EDTA tubes, fresh blood leaves little to make blood smear, total blood examination conducted with automatic hematological analyzer ABX MICROS 60 and for total leukocyte and differential leukocyte count used standard manual method. The mean result of hematological value of leopard gecko in this research are WBC (x103/mm3) 7,12 male, 8,09 female, Lymphocyte (%) 46,2 male, 38,4 female, Monocyte (%) 17 male, 17,3 female, Heterophil (%) 18,3 male, 21,8 female, Eosinophil (%) 10,8 male, 13,9 female, Basofil (%) 7,7 male, 8,6 female, RBC (106/mm3) 0,64 male, 0,65 female, HGB (g/dl) 8,95 male, 9,46 female, Hematocrit (%) 10,55 male and 9,26 female. In all parameters showed that hematology value of male gecko leopard was lower than female gecko except on two parameters which are hematocrit and lymphocyte which showed male gecko has the higher value than females, but the hematology value difference of both sexes leopard gecko is not significant except in heterophil value. 
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST HISTOPATHOLOGICAL CHANGES OF SMALL INTESTINE ON MALE WHITE RAT (Rattus norvegicus) WISTAR STRAIN Amaris, Aulia Puspa; Yustinasari, Lita Rakhma; Legowo, Djoko; Plumeriastuti, Hani; Anwar, Chairul; Hidajati, Nove
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20409

Abstract

This study was conducted to investigate the effect of polypropylene residue on heated palm oil against histopathological changes of small intestine on male white rat (Rattus norvegicus) wistar strain. Eighteen male white rats divided into three groups and were administered via peroral gavage with different treatments for five weeks. The treatment were Control (84 grams palm oil), P1 (84 grams palm oil with 2.3 grams plastic), and P2 (84 grams palm oil with 6.81 grams plastic). The histopathological changes of small intestine were examined by using Barthel Scoring method was analysed using Kruskal Wallis and continued with Mann-Whitney test. The result showed that were significant difference (p<0.05) between treatment groups. The worse damage of polypropylene plastic residue was 6.81 grams.
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST THE HISTOPATHOLOGICAL CHANGES OF KIDNEY ON MALE RATS (Rattus norvegicus) Cahyono, Shahani Azpriyanne; Eliyani, Hana; Widiyatno, Thomas Valentinus; Plumeriastuti, Hani; Hidajati, Nove; Santoso, Kuncoro Puguh
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20415

Abstract

This research was conducted to observe the effect of polypropylene plastic residue on heated palm oil against the histopathological changes on rat's kidney. There were 18 male rats (Rattus norvegicus) that used as the examination animal and were divided into three groups and were given the mixture of heated palm oil and polypropylene plastic per oral with intubation needle. The treatment was done for five weeks. The treatment for P0 group was 84 g of palm oil, for P1 was 84 g of palm oil with 2.3 g of plastic, and for the P2 was 84 g of palm oil with 6.8 g of plastic. The histopathological changes of the kidney were observed using microscope with 400x magnification and the data was analysed using Kruskal-Wallis and continued with Mann Whitney U test. The result showed that there were significant differences between three groups of treatment (P<0.05). There were effects of polypropylene plastic residue on heated palm oil against the histopathological changes of kidney in this research such as cast, epithelial sloughing and necrosis in the mild scale.
Identification of Cat (Felis catus) Blood Splatter on Cotton Fabric After Periods of Drying Using Leucomalachite Green and Takayama Reagent Charles, Vanessa Ann; Lestari, Tita Damayanti; Legowo, Djoko; Ismudiono, Ismudiono; Hidajati, Nove; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28593

Abstract

Blood-stain or blood splatter analysis when used properly can assist in establishing a chain of events linked to violent crimes (Bevel and Gardner, 2008). The methods used in detecting blood splatters in the field are chemical methods. Leucomalachite green is a colorimetric test which is used to test the presence of blood (Castro and Coyle, 2008). Takayama reagent is a confirmatory test for blood (Strassman, 1922). The aim of this research is to detect the blood splatter on cotton fabric after it has been dried for 1 day, 3 days and 5 days using Leucomalachite green and Takayama reagent. Cotton fabric was specifically chosen for this experiment with 3 different periods of drying. The unstained cotton fabric was cut into squares, and a blood sample was splattered on each piece. The fabrics splattered with blood were then dried for 1 day, 3 days and 5 days. The blood splatter was then tested using Leucomalachite green and Takayama reagent, and the results were noted afterwards. For the control, red food dye was dried for 1 day then tested with Leucomalachite green and Takayama reagent. The image results of the Leucomalachite green test are analyzed using ImageJ software 1.8.0_112 where the red, green and blue pixels are converted to grayscale. The image results of the Takayama test are graded based on the number and pattern of crystal. In conclusion, Leucomalachite green and Takayama reagent are able to detect cat blood splatter on the cotton fabric. Leucomalachite green produced a higher intensity/ darker colour as a result of an older sample, and the lower intensity/ lighter colour as a result of a fresher sample of the Leucomalachite green test. Takayama reagent produced a densely packed pattern of crystals as a result of an older sample, and the loosely packed pattern of crystals as a result of a fresher sample of the Takayama test.
Toxicity Testing Of White Pomegranate (Punica granatum L.) Fruit Extracts Using Brine Shrimp Lethality Test Method As A Candidate Of Anti-Cancer Drug Firnanda, Ferian; Herupradoto, Eduardus Bimo Aksono; Rahmawati, Kadek; Kurnijasanti, Rochmah; Sukmanadi, Mohammad; Hidajati, Nove
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31810

Abstract

This research aimed to determine the effects of toxic white pomegranate fruit extract (Punica granatum L) against larvae of brine shrimp (Artemia salina Leach) indicated LC50 values below 1000 µg/ml. This study is purely experimental by using Brine Shrimp Lethality Test (BSLT). The study was divided into seven groups, namely ethanol extract of white pomegranate fruit (Punica granatum L) with a concentration of 31,25; 62,5; 125; 250; 500; 1000 µg/ml and negative control (seawater). Mortality data percentage of Artemia salina Leach analyzed by probit analysis. Results showed that the extract of white pomegranate fruit extract (Punica Granatum L) has a toxic effect with LC50 values of 248,6 µg/ml calculate from probit analysis. From these results, it can conclude that white pomegranate extract is toxic to larval shrimp (Artemia salina Leach) with Brine Shrimp Lethality Test (BSLT) method which means white pomegranate extract has the potential to be an anticancer drug.
The Effect of Kemangi Leaf (Ocimum Basilicum) Extract in The Egg Yolk Skim Milk Diluent on The Plasma Membrane and Motility of Boerawa Goats' Sperm Farah Ghifara Hafidha; Utomo, Budi; Soeharsono; Lestari, Tita Damayanti; Hidajati, Nove; Hermadi, Herry Agoes
Media Kedokteran Hewan Vol. 36 No. 3 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i3.2025.273-281

Abstract

The purpose of this research was to determine the effect of kemangi leaf (Ocimum basilicum) extract in the egg yolk skim milk diluent on the plasma membrane and motility of Boerawa goats' sperm at room temperature. The research used fresh samples of Boerawa goat semen collected by artificial vagina. The experimental design used was a completely randomized design, divided into four treatments with six replications. The treatments were the addition of kemangi leaf extract at the levels of 0% (P0), 1% (P1), 2% (P2), and 3% (P3). Diluted semen was stored at room temperature and observed at hours 0, 1, 2, and 3. Analysis of the data using Multivariate Analysis of Variance (MANOVA), then proceed to the Duncan Test to determine significant differences between treatments. The results showed that there was no significant difference (p > 0.05) in sperm plasma membrane, but a significant difference (p < 0.05) in sperm motility was observed when kemangi leaf extract was added. However, the addition of kemangi leaf (Ocimum basilicum) extract tends to provide a better value than without it. The addition of kemangi leaf extract at a concentration of 2% yields the highest results in sperm plasma membrane integrity and sperm motility of Boerawa semen at room temperature.
PENGARUH PEMBERIAN EKSTRAK KULIT SEMANGKA (Citrullus lanatus) TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA TIKUS (Rattus norvegicus) DENGAN PAPARAN SUHU PANAS Rohmah, Lailatul; Triana, Indah Norma; Sunarso, Agus; Susilowati, Suherni; Hidajati, Nove; Kurnijasanti, Rochmah
Ovozoa: Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.302 KB) | DOI: 10.20473/ovz.v7i2.2018.131-136

Abstract

High temperatures lead to oxidative stress which can decrease the quality of spermatozoa. Watermelon's rind have an ability for antioxidants effect that can reduce sperm's damages that results by role explanation of free radicals. This study aimed to determine the effect of extract of watermelon's rind on the motility and viability of sperm on male rat exposed to heat. This experiments use rat as animals experiment with five treatments and four repetitions. Five treatments are: P0: without exposured to 40ËšC of temperature and without treatment by extract watermelon's rind, P1: was exposured to 40ËšC of temperature and without treatment by extract watermelon's rind, P2: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 20 mg/rat/day, P3: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 40 mg/rat/day, and P4: was exposured to 40ËšC of temperature and treatments by extract watermelon's rind dosage 80 mg/rat/day. The treatment was done for 52 days. The results, P4 have the best effect that can be increase the motility and viability of rat's sperm (Rattus norvegicus) exposed to heat.