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Bioscientist : Jurnal Ilmiah Biologi
Published by Universitas Pendidikan Mandalika
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Core Subject : Science, Education, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Social Sciences
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,452 Documents
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Isolation and Characterization of Protease-Producing Thermophilic Bacteria from Padang Dama Hot Spring, Solok, Indonesia Putri, Mellanie Alia; Agustien, Anthoni; Marlida, Yetti; Alamsjah, Feskaharny
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20118

Abstract

This study aimed to isolate, screen, and partially characterize protease-producing thermophilic bacteria from the Padang Dama hot spring, Solok, Indonesia. The main issue addressed in this study is the limited information regarding thermophilic bacteria capable of producing protease enzymes from this geothermal environment, despite their potential use in industrial processes requiring stable enzymes. Isolation was carried out using Nutrient Agar followed by incubation at 50 °C for 24 hours. Screening of protease activity was performed using Skim Milk Agar based on clear zone formation, and the proteolytic index (PI) was calculated to evaluate enzyme activity. Selected isolates were further characterized through macroscopic, microscopic, and biochemical analyses. The results showed that three isolates exhibited proteolytic activity, with isolate TUA-109 showing the highest proteolytic index, indicating superior enzymatic potential compared to other isolates. Partial characterization revealed that isolate TUA-109 is a Gram-negative, rod-shaped bacterium with positive catalase activity and motility, which are important traits supporting its adaptability and potential for enzyme production under extreme conditions. These findings highlight the potential of thermophilic bacteria from the Padang Dama hot spring as sources of protease enzymes, with isolate TUA-109 identified as a promising candidate for further investigation, particularly for industrial enzyme production. Future studies are recommended to optimize enzyme production and evaluate its application in industrial processes.
Effect of Dietary Supplementation with Green Spirulina on the Survival Rate and Color Intensity of Goldfish (Carassius auratus) Marpaung, Muhd. Rizky; Afriani, Dwi Tika; Siswoyo, Bambang Hendra
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20158

Abstract

This study aimed to analyze the effect of adding natural carotenoid sources to feed on the enhancement of color intensity and survival rate of fish. The study employed a Completely Randomized Design (CRD) with four treatments and three replications, namely P1 (control), P2 (6% spirulina), P3 (8% spirulina), and P4 (10% spirulina). The experiment was conducted for 30 days using 60 experimental fish. The observed parameters included color intensity and survival rate. Data were analyzed using a one-way ANOVA. The results showed a significant difference among treatments on fish color intensity (P < 0.05). Treatment P3 (8% spirulina) produced the highest color intensity value (21.12 ± 0.27), followed by P4 (10%) (20.76 ± 0.19) and P2 (6%) (20.66 ± 0.07), while the lowest value was observed in P1 (control) (20.55 ± 0.14). However, no significant difference was found in the survival rate (P > 0.05), with the highest value in P1 (93%) and the other treatments showing similar values (87%). The addition of spirulina significantly improved fish color intensity, with the 8% dosage being the most effective treatment, but it did not significantly affect the survival rate.
Analysis of Geographic Environmental Risk Factors Associated with Dengue Infection in Palembang City Randana, Muhammad Prima Cakra; Irfannuddin; Damiri, Nurhayati; Anwar, Chairil
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20161

Abstract

This study aimed to identify the relationship between environmental factors temperature, humidity, and rainfall, and dengue infection incidence in Palembang City, with emphasis on temporal lag effects and their implications for disease surveillance and control. A quantitative descriptive study was conducted using monthly secondary data from 2021 to 2023. Time-series descriptive analysis and lag correlation methods were applied to examine both concurrent and delayed associations between the environmental variables and dengue case counts. While temperature and humidity showed no consistent concurrent association with dengue incidence, rainfall demonstrated a notable lag-1 effect, whereby increased rainfall in a given month was followed by a rise in dengue cases one month later. This temporal pattern suggests that post-rainfall accumulation of standing water may facilitate mosquito breeding and subsequently elevate transmission risk. These findings highlight the importance of incorporating temporal environmental indicators into dengue early warning systems. Integrating rainfall lag patterns into public health monitoring could support more timely and targeted prevention efforts in Palembang and comparable urban settings across Indonesia.
Metagenomic Analysis of Bacterial Communities in Batik Wastewater from Zie Semarang Safitri, Diah Rahma; Susanti, R.
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20180

Abstract

This study aims to analyze the abundance profile, diversity of bacterial communities, and their potential as bioremediation agents in batik wastewater. The research method includes analysis of heavy metal levels (Cr, Pb, Cd, and Cu) using the Atomic Absorption Spectrophotometer (AAS) method and metagenomic analysis based on Next Generation Sequencing (NGS) in the target region V3-V4 of the 16S rRNA gene. The AAS test results showed a chromium (Cr) concentration of 0.035 ppm. The metagenomic analysis results produced alpha diversity, namely the Shannon index of 2.749 and Simpson of 0.865, which means that bacterial diversity is in the moderate category with the dominance of certain groups. The bacterial community was dominated by the phyla Pseudomonadota (62.38%) and Actinomycetota (28.61%), which are known to have high adaptation and tolerance to Cr in degrading organic compounds. At the genus level, the highest percentages were found in Rickettsia (30.2%) and Xanthobacter (24.58%). Xanthobacter has potential as a bioremediation agent, but the presence of Rickettsia needs further study because the sample was mixed with other sources containing Rickettsia vectors. This study provides a basis for optimizing bioremediation by utilizing bacteria that have potential as pollutant degraders based on analysis of previous studies.
Medicinal Plant Species in Lakuan Buol Village, Lakea District, Buol Regency, as Learning Media Septiani, Wahyu Indah; Shamdas, Gamar B. N.; Febriani, Vita Indri; Alibasyah, Lestari M. P.; Febriawan, Aan; Nurdin, Musdalifah
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20183

Abstract

This study aims to identify medicinal plant species, describe the plant parts used and their processing methods, and develop learning media in the form of a pocket book and flipbook in Lakuan Village, Lakea Sub-district, Buol Regency. This research used a descriptive qualitative approach through field observations, interviews with key informants (traditional healers) and supporting informants (medicinal plant users), as well as documentation. Data were analyzed descriptively by grouping plant species, used parts, and processing techniques, supported by literature review. The results showed 32 species from 24 families, with specific plant parts used, namely leaves for fever and wounds due to their ease of processing, roots for internal diseases, rhizomes as anti-inflammatory and antibacterial agents, stems for relaxation, fruits for digestion and immunity, and flowers for cough treatment. The pocket book and flipbook media were chosen because they are practical, concise, and interactive, making it easier for students to understand the material. The feasibility test results indicated that the media was categorized as highly feasible as a context-based learning resource.
Evaluation of the Effectiveness of Turmeric (Curcuma longa) Extract as a Feed Additive on the Growth and Survival of Common Carp (Cyprinus carpio) Kusuma, Argi; Siswoyo, Bambang H.; Manullang, Helentina M.
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20191

Abstract

This study aimed to evaluate the effect of dietary turmeric extract supplementation on the growth performance and survival rate of common carp. The study was conducted using an experimental method with a completely randomized design (CRD) consisting of four treatments and three replicates: P1 (control), P2 (5% turmeric extract), P3 (10% turmeric extract), and P4 (15% turmeric extract). The observed parameters included weight gain, length gain, survival rate, and water quality variables, namely temperature and pH. The results showed that turmeric extract supplementation had no significant effect (p > 0.05) on the weight or length growth of common carp. However, descriptively, a positive growth trend was observed at the 5% supplementation level, whereas increasing the dose to 15% tended to reduce fish survival. Throughout the study, water quality parameters remained within the tolerance range for common carp and were not considered limiting factors. These findings indicate that turmeric extract was not yet effective as a primary growth promoter; however, it may have potential as a supportive feed additive when applied at low doses. Further studies are recommended to determine a more specific optimal dosage and to include physiological parameters and feed efficiency indices to obtain more comprehensive results.
Implementation of Culturally Responsive Teaching-Based Problem-Based Learning on Students’ Analytical Thinking Skills in the Digestive System Topic in Grade XI at SMAIT Nur Hidayah Nurlaili, Ni'matun; Astuti, Dwi Setyo
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20264

Abstract

This study aimed to examine the effectiveness of a Problem-Based Learning (PBL) model integrated with Culturally Responsive Teaching (CRT) in enhancing students’ analytical thinking skills in learning the digestive system. A quantitative approach was employed using a quasi-experimental research design. Specifically, the study adopted a non-equivalent control group design with purposive sampling, involving 28 students in the experimental class and 28 students in the control class. Data were collected through pretest and posttest assessments and were analyzed using an independent samples t-test. The results showed that the mean posttest score of the experimental class (84.82) was higher than that of the control class (71.43), with a significance value of p < 0.001, indicating a statistically significant difference between the two groups. These findings suggest that the integration of Problem-Based Learning (PBL) and Culturally Responsive Teaching (CRT) is effective in improving students’ analytical thinking skills through culturally contextualized learning. This study contributes to the development of biology instruction grounded in local contexts, an area that remains relatively underexplored.
Comparative Analysis of Insect Pest Diversity in Cocoa (Theobroma cacao L.) Agroecosystems under Different Shade Tree Canopies: Papaya vs. Banana Mubarok, Basroh Saepul; Kusuma, Ramadhani Mahendra; Rahmadhini, Noni
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20289

Abstract

This study compared the pest insect community structure in cocoa plantations shaded by papaya and banana trees. The study was conducted from January to March 2025 using systematic sampling in six cocoa fields, consisting of three papaya-shaded and three banana-shaded plantations. In each field, 19 traps were installed, comprising nine yellow traps, nine pitfall traps, and one light trap, supported by visual observations. Pest insects were identified to the genus level, and community structure was assessed using diversity (H′), evenness (E′), richness (R′), and dominance (C′) indices. Banana-shaded cocoa fields supported slightly more pest insect genera than papaya-shaded fields, with 22 and 21 genera recorded, respectively. Banana-shaded plots also showed higher diversity, evenness, and richness, accompanied by lower dominance. These patterns indicate that the denser banana canopy may create more heterogeneous microhabitat conditions that support a broader range of pest insects. Shade tree type influences pest insect community structure in cocoa plantations. Banana shade was associated with a more diverse and balanced pest insect community, whereas papaya shade showed relatively higher dominance, suggesting a greater risk of specific pest outbreaks. These findings highlight the importance of shade tree selection in integrated pest management, particularly for designing site-specific monitoring and targeted control strategies in cocoa plantations.
Processing of Sugarcane Bagasse (Saccharum officinarum L.) for Bioplastic Production with the Addition of Glycerol and Chitosan Lubis, Annisa Nurul Sakinah; Hutasuhut, Melfa Aisyah; Widiarti, Leni
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20291

Abstract

This study aimed to analyze the effects of varying bagasse mass, as well as the roles of glycerol as a plasticizer and chitosan as a reinforcing agent, on the physical, mechanical, thermal, and morphological properties of bioplastics. The research was conducted through laboratory experiments using a completely randomized design (CRD). The mass of bagasse was varied at 2, 4, 6, 8, and 10 g, while the concentrations of glycerol and chitosan were kept constant across all treatments. The results showed that increasing the mass of bagasse tended to produce bioplastics with greater stiffness and brittleness, while also reducing surface homogeneity, as observed through SEM analysis. The addition of glycerol improved material flexibility, as indicated by an increase in elongation from 11.8% to 27.9%. Meanwhile, chitosan contributed to enhanced tensile strength, which increased from 0.4199 MPa to 1.4221 MPa. In addition, higher bagasse content improved thermal resistance, as demonstrated by DSC analysis. The highest biodegradation rate reached 73.95% in a sample with a specific composition during the testing period. FTIR analysis confirmed the presence of functional group interactions among cellulose, glycerol, and chitosan within the bioplastic matrix. These findings indicate that the combination of bagasse with glycerol and chitosan can produce bioplastics with a balanced combination of flexibility, mechanical strength, and biodegradability. This study contributes to the utilization of agricultural waste as a value-added raw material for bioplastic production and supports the development of sustainable, environmentally friendly materials.
The Implementation of the Assemblr Edu AR: Biodigest Application on Student Learning Outcomes in Digestive System Material at SMA Batik 2 Surakarta Mawarni, Nur Fatikhah; Aryani, Ima
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2026): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i2.20293

Abstract

This study aimed to examine the effect of implementing the Assemblr Edu AR: BioDigest application on students’ cognitive learning outcomes and to evaluate student engagement in the affective and psychomotor domains during instruction on the digestive system in Grade XI at SMA Batik 2 Surakarta. This study employed a quasi-experimental method using a nonequivalent control group design. The research sample consisted of two classes: XI EIPA 1 as the control class, which used PowerPoint media, and XI EIPA 2 as the experimental class, which used the Assemblr Edu AR: BioDigest application. Data were collected through learning achievement tests and validated observation sheets, and were analyzed using an independent samples t-test and N-gain analysis. The results showed a significant difference between the experimental and control classes in cognitive learning outcomes (p < 0.05). The effectiveness of the instructional media in the experimental class was indicated by an N-gain score of 0.5944, which falls within the moderate category. In the affective and psychomotor domains, the experimental class also demonstrated higher levels of engagement than the control class. These findings indicate that three-dimensional visualization and interactive features in augmented reality can enhance conceptual understanding, learning motivation, and student skills by providing a more meaningful learning experience. Theoretically, this study contributes to strengthening research on augmented reality-based learning. Practically, it offers an innovative alternative instructional medium for biology education, particularly for abstract and complex topics.

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