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INDONESIA
Bioscientist : Jurnal Ilmiah Biologi
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Articles 1,428 Documents
Tren Kejadian Dengue (Incidence Rate) dan Kematian Akibat Dengue (Case Fatality Rate) di Indonesia Meyrita, Meyrita; Suwarno, Suwarno; Saidi, Nurdin; Razi, Nanda Muhammad
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9500

Abstract

Dengue Hemorrhagic Fever (DHF) is a disease that currently has significant morbidity and mortality rates. In Indonesia, the trend of DHF cases has increased and decreased every year. This study aims to analyze the development of dengue cases by considering two indicators, namely dengue incidence rate and dengue case fatality rate (CFR) during the period 2012-2022, to know the pattern of dengue disease in Indonesia, and evaluate the government's target in handling dengue cases based on IR and CFR rates. The data used is secondary data obtained from the 2021 Indonesian Health Profile and the 2022 Dengue Fever Annual Report. IR and CFR data were analyzed using Microsoft Excel which will be interpreted in the form of graphs to illustrate the trends and patterns of DHF cases in Indonesia, IR maps in 2021 and 2022 were analyzed using ArcGIS software. Based on the results of the analysis, the development of dengue cases during the period 2012-2022 experienced a misalignment between the increasing trend of IR and CFR. The IR value experienced significant fluctuations from 37.3 per 100,000 population in 2012 to 52.07 per 100,000 population in 2022, while the CFR value during the period 2012-2022 tended to decrease from 0.90% (2012) to 0.86% (2022). This pattern reflects the complex dynamics of DHF epidemiology, where an increase in cases is not always followed by an increase in fatalities. The IR and CFR achievements for 2022 have not met the government's expectations, with only 16% of districts/cities having IR values ≤ 10/100,000, while the CFR value reached 0.86%, slightly above the set target of 0.7%.
Analisis Molekuler Tilapia Lake Virus (TiLV) di Sentra Budidaya Benih Ikan Gurami (Osphronemus goramy) Jawa Timur Jumria, Andi; Amin, Muhamad; Mufasirin, Mufasirin
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9515

Abstract

Tilapia Lake Virus (TiLV) is a New Emerging Disease that has been defined and included in the WOAH disease list (WOAH, 2022). This virus can infect both freshwater and marine fish, causing high mortality in Ttilapia up to 90%. Reverse transcyptase method - Semi Nested Polymerase Chain Reaction (RT-snPCR) for detection of TiLV in Osphronemus goramy. TiLV was discovered for the first time in Thailand and was also found in Jogjakarta in 2021. Further research is needed to see the damage and genetic variation caused by TiLV in Osphronemus goramy. This study aims to study the genetic variability and homology of TiLV isolates in Osphronemus goramy as definitive hosts in East Java. The results showed clinical symptoms of lethargic fish, decreased appetite, abnormal behavior, reduced reflexes, sluggish movements, darker body color, congestion, thinning fins and exopthalmia. The PCR results showed that the Osphronemus goramy showing clinical symptoms of TiLV infection were all positive. The sequencing results were further identified using the BLAST program, showing the highest similarity of the genetic identity of the sample at 96.8% with TiLV from Israel (Genebank Accession Number NC 029927.1).
Meta-Analisis : Studi Pentingnya Mengukur Keterampilan Metakognitif Siswa Hajiriah, Titi Laily; Sudiatmika, AAIA Rai; Suma, I Ketut; Suardana, I Nyoman
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9567

Abstract

This study aims to find the effect size of several journals that contain articles with uniform topics. The purpose of finding effect size is to measure the extent of differences or relationships between two or more variables or groups in scientific research. Effect size provides information regarding the strength and size of the observed effect, regardless of the sample size used. By evaluating effect size, researchers can determine the practical significance of their findings and compare the results of studies conducted by other researchers. The key word of this meta-analysis study is metacognitive skills. This meta-analysis is important to get an idea of studies that report the results of measuring metacognitive skills at various levels. The research method used in this study is a meta-analysis method with an experimental approach to journal tracking using OpenMEE software with keywords used, namely metacognitive skills, metacognition, metacognition awareness. Based on meta-analysis calculations that there is a relationship between all metacognitive skill measurement articles, a p value of < 0.001 means that the analysis is significant with sig. 0.05 and I^2 above 80% indicate that heterogeneity of learning measures metacognitive skills of 93.985 and the sample effect size in this study is very high heterogeneity.
Aktivitas Antioksidan dan Karakterisasi Nanopartikel Ekstrak Kulit Salak Wedi sebagai Kosmetik Antiaging Saputri, Romadhiyana Kisno; Al-Bari, Akhmad; Nisak, Siti Khoirun; Anti, Tika Roro; Amelia, Rika
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9590

Abstract

Wedi snakefruit is one of the local species snakefruit produced in Bojonegoro Regency. Antioxidant activity in salak fruit may be influenced by its cultivar. The peel of snakefruit is reported to have antioxidant activity as in salak fruit flesh. Antioxidants can prevent free radical damage to human skin and prevent aging so can be developed in cosmetic preparations. The current trend of antiaging cosmetic preparations leads to preparations with nanoparticle size. The objectives of this study is to determine the antioxidant activity and to characterisation of nanoparticles Wedi snakefruit peel extract that will be developed as anti-aging cosmetics. Wedi snakefruit peel extract was conducted using maceration method with 96% ethanol solvent, antioxidant activity test was conducted by DPPH method and total phenol content determination method using Folin-Ciocalteu method. Nanoparticles preparation of extract by ionic gelation method with characterisation through particle measurement using PSA and zeta potential measurement. Potential antiaging activity was measured through tyrosinase inhibition testing. The results showed the IC50 value measured by DPPH method of 56.10 ppm, total phenolic content of 13.41 mg GAE/g. PSA test results showed particle size of Wedi snakefruit peel extract 884 nm and zeta sizer value of 2 mv. Tyrosine inhibitory activity of Wedi snakefruit peel extract was 63.8%. The antioxidant activity of Wedi snakefruit peel extract is in the strong category, has not met the nanoparticle requirements and has tyrosinase inhibitory activity so that it has the potential to be developed as an antiaging cosmetic.
Analisis In Silico : Aktivitas Antiinflamasi dan Prediksi Toksisitas Senyawa Metabolit Sekunder Daun Ciplukan (Physalis peruviana L.) Fadzillah, Sania Nur; Hernawati, Diana; Putra, Rinaldi Rizal
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9592

Abstract

The way of life back to nature, including in the field of health, forms the habit of using plants as traditional medicine. Traditional medicine is indicated to be able to cure a variety of diseases. As with the leaves of Ciplukan (Physalis peruviana L.) by the people of Desa Wanasuka Kecamatan Pangalengan Kabupaten Bandung is considered to be used as a traditional medicine for inflammatory diseases such as rheumatism, asthma, and gastritis (maag) due to side effects and toxicity produced relatively small when compared with modern medicine. The aim of this study is to identify the potential of the secondary metabolite compound found in the leaves of the Ciplulan (Physalis peruviana L.) as a candidate for anti-inflammatory drugs. These research methods are molecular methods of docking through physical chemical properties testing, pharmacokinetic profiles of test compounds through site Pre-ADMET (Absorption, distribution, metabolism, excretion, and toxicity) and Lipinski Rule of Five. The results of this study showed that the binding affinity of caffeic acid compounds with PTGS2 receptors (PDB ID: 5F19) showed a more negative result compared to the comparative drugs Aspirin and Ibuprofen -7.4. There was a molecular interaction similar to Aspirin, in which the addition of amino acid residues from ASN 382 and TYR 385 produces a strong irreversible inhibition of PTGS2 (PDB ID: 5F19) and is predicted to inhibit prostaglandin H synthase and indicate that caffeic acid is capable of producing irreverible inactivation of PTGS2 (PDB ID:5F19). So potentially a candidate for a safer anti inflammatory drug for the body. Because it has almost the same or close potential as Aspirin against inflammatory diseases.
Perbandingan Pola Rasio Morfometrik dan Karakteristik Habitat Dua Spesies Ikan Cupang Alam (Betta spp.) Endemik Pulau Bangka Ramadhanu, Destra; Prananda, Mustobi; Wulandari, Ufi Ayu; Hidayat, Rahmad; Syarif, Ahmad Fahrul
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9598

Abstract

Bangka Island has extraordinary biodiversity. Bangka Island has various types of freshwater fish, including local and endemic fish. The natural betta fish Betta burdigala and Betta chloropharynx are two species of fish endemic to Bangka Island which are considered endangered. The aim of this study was to analyze the differences in morphology and habitat characteristics between these two species. This research uses a quantitative descriptive approach. The fish samples used were 10 Betta burdigala and Betta chloropharynx fish taken from Bikang Village, South Bangka Regency and Jade Bahrin Village, Bangka Regency from August to September 2023. The data obtained were analyzed descriptively and presented as graphs or tables. There are eight main characteristics based on morphometric ratios, namely Total Length (TL), Body Length (BL), Tail Length (TL), Head Length (HL), Head Height (HH), Body Width-I (BW-I), Body Width-II (BW-II), and Body Width-III (BW-III). Based on morphometric ratios, there are differences in all morphometric characters. Betta burdigala and Betta chloropharynx live in peat swamp waters, the pH of Betta burdigala water is lower than the pH of Betta chloropharynx water, the color of Betta burdigala water is more black brown, while the color of Betta chloropharynx water is clearer.
Dampak Pengolahan Air Lindi terhadap Kualitas Air Sungai dan Sumur di Sekitar TPA Regional Piyungan Yogyakarta Dari, Harnung Wulan; Suhartini, Suhartini
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9601

Abstract

Water is the main need for life, so the quality of the water used in life needs to be known. The objectives of this research are to determine the leachate management system at the IPL, determine efficiency of leachate water treatment performance at IPL, and determine impact of leachate water quality at Piyungan Regional Landfill on the river and wells water quality around the landfill. This research is quantitative descriptive research conducted at Piyungan Regional Landfill and its surroundings in April-July 2023. The data collected were results of physical, chemical, and biological parameters of the IPL Inlet and Outlet leachate water quality, Opak River after the IPL Outlet, and 3 samples of well water from the surrounding community representing distance from the landfill. The data collection methods used are observation, documentation, and analysis of water quality in the laboratory. The data obtained were analyzed using descriptive methods and compared with quality standards set by the Government. The results showed that: The leachate water management system at the IPL uses 8 water treatment tanks include: a bar screen, inlet, coagulation tank, equalization tank, sedimentation tank, SDB, ABR, aeration tank, maturation tank, chlorination tank, and Outlet. The efficiency of leachate water treatment performance at the IPL is not effective and efficient in processing leachate water. The quality of leachate water affects the water quality of the Opak River and wells around the landfill, on chemical parameters TDS, BOD, COD, DO, and microbiological parameters Total Coliform, therefore, the Regional Government need to evaluate and improve the IPL. Communities around the Landfill that use well water as their main need must be filtered and boiled.
Validation of Genetic Markers for AmpC, AAC, and RNR3 Genes in Potentially Present Pathogens Organisms in Fecal Sludge Musyahadah, Ummu Syauqah Al; Hamid, Radhiyah Mardhiyah; Nasir, Alricha
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9623

Abstract

The issue of sanitation and water pollution due to fecal waste has become a serious concern in Indonesia, particularly after it was revealed that approximately 70% of the community's drinking water sources are contaminated with human feces. Determining safe waste management requirements necessitates indicators confirming the contamination-free nature of the waste management methods. Pathogen testing indicators for fecal waste are highly diverse. The current standard testing is the culture-based method for microorganisms and microscopy observation for helminths. However, molecular detection tools for these pathogens can give higher sensitivity and specificity in a relatively shorter time of technical processing. In this research, we designed primers for molecular-based diagnostics on three pathogens exhibiting extreme survival capabilities under various environmental conditions. The species-coupled genes are AmpC for Escherichia coli, AAC for Mycobacterium tuberculosis, and RNR3 for Ascaris lumbricoides. The design of molecular-based biomarkers and their specificity tests were conducted using web-based bioinformatics programs to prevent unintended dimers. Ultimately, primers were validated by conventional gradient PCR on available positive controls and a sample from processed fecal sludge. The PCR validation test showed that the designed primers were validated to amplify the intended target with the right size without unspecific bands on the positive controls. Meanwhile, the AAC gene for E. coli was detected in the sample from the fecal sludge.
Pengembangan Asessment Higher Order Thinking Skills (HOTS) Berbasis Computer Based Test (CBT) pada Mata Kuliah Prinsip Pengajaran dan Asesmen yang Efektif Tibrani, Mgs; Madang, Kodri; Anggraini, Nike
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9639

Abstract

The aim of this research is to produce a Higher Order Thinking Skills (HOTS) assessment product based on a Computer Based Test (CBT) with three types of HOTS questions (true or false, multiple choice, and fill in the blank and matching) based on the factual knowledge dimension, conceptual, procedural, and metacognitive with illustrations in the form of discourse, images, graphics, videos, animations, and interactive stimulation, as well as providing feedback after going through the logical validation, empirical validation, and respondent questionnaire stages. The CBT-based HOTS assessment product was implemented for PPG students, Faculty of Teacher Training and Education, Sriwijaya University in the Principles of Effective Teaching and Assessment course with R&D research steps. The product trial subjects in this research were limited to a small scale, namely 8 PPG Pre-Service Biology Education Study Program students, FKIP, Sriwijaya University. The technique for selecting product trial subjects in this research is the cluster sampling technique, namely a sampling method based on certain clusters. The data collection technique uses expert validation sheets and respondent questionnaires, then the data collected is analyzed quantitatively descriptively. Data obtained from questionnaires and scientific communication skills were analyzed using a rating scale. To analyze each statement or indicator, the frequency of answers for each category (answer choices) is calculated and added up. The development of HOTS questions based on the Computer Based Test in the Principles of Effective Teaching and Assessment course is based on two validation values from the validator, namely 81%, meaning that the material and language that has been designed by the researcher is valid and suitable for use. Apart from that, HOTS test questions are said to be practical if the score on the practicality criteria is at least 75%. Based on the results of the student response questionnaire, a score of 91.81% was obtained.
Identifikasi Teripang Laut (Holothuroidea) dari Perairan Laut Pulau Kabaruan Berdasarkan Identifikasi Morfologi dan DNA Barcoding Rattu, Ferencia Esananda; Mege, Revolson; Manampiring, Nonny; Roring, Verawati Ida Yani; Gedoan, Sukmarayu; Mokosuli, Yermia
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9649

Abstract

Indonesia is known with its high biodiversity. One of them is sea cucumber. Various species of sea cucumbers are found in Indonesian sea in Talaud Regency at Kabaruan Island. Identification of sea cucumbers to reveal the biodiversity of sea cucumbers in Kabaruan Island, which is almost unknown. This research was to identify six samples of sea cucumber (Holothuroidea) in Kabaruan Island based on morphology and DNA barcoding using the COI gene. The differences between each species can be seen from the morphological characteristics, the length and width of sea cucumbers, papillae, pattern and color. The results showed that there were 4 species from 6 samples, which is Holothuria leucospilota, Holothuria whitmaei, Eleutherozoa sp., and Holothuria atra. That six samples with the code A1, A2, B1, C1, and C2 have a homology level of 99%-100% with the target nucleotide sequence from GenBank. Meanwhile, the sample’s code A3 has a homology level of 90% with the Eleutheroza sp. so there is a possibility that sample A3 is a new taxa that has the highest similarity to the Eleutheroza sp. From the six samples, it was found that samples A2 and B1 had the highest level of similarity which is 100%. Meanwhile, the samples with the lowest percentage values are samples A3 and B1 and samples A2 and A3, with a percentage value of 82.75%. There were 4 species from six samples found in Kabaruan Island. There is a possibility that Sample A3 is a new species that is similar to Eleutheroza sp.

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